1. Binding of human milk to pathogen receptor DC-SIGN varies with bile salt-stimulated lipase (BSSL) gene polymorphism.
- Author
-
Stax MJ, Naarding MA, Tanck MW, Lindquist S, Hernell O, Lyle R, Brandtzaeg P, Eggesbø M, Pollakis G, and Paxton WA
- Subjects
- Breast Feeding adverse effects, CD4-Positive T-Lymphocytes immunology, CD4-Positive T-Lymphocytes metabolism, CD4-Positive T-Lymphocytes virology, DNA Repeat Expansion genetics, DNA Repeat Expansion physiology, Egypt, Female, Genetic Predisposition to Disease, Genotype, HIV Infections immunology, HIV Infections metabolism, HIV-1 metabolism, HIV-1 physiology, Humans, Infant, Newborn, Lipase metabolism, Maternal Exposure adverse effects, Milk, Human physiology, Milk, Human virology, Netherlands, Norway, Protein Binding genetics, Sweden, Cell Adhesion Molecules metabolism, HIV Infections genetics, HIV Infections transmission, Lectins, C-Type metabolism, Lipase genetics, Milk, Human metabolism, Polymorphism, Genetic physiology, Receptors, Cell Surface metabolism
- Abstract
Objective: Dendritic cells bind an array of antigens and DC-SIGN has been postulated to act as a receptor for mucosal pathogen transmission. Bile salt-stimulated lipase (BSSL) from human milk potently binds DC-SIGN and blocks DC-SIGN mediated trans-infection of CD4(+) T-lymphocytes with HIV-1. Objective was to study variation in DC-SIGN binding properties and the relation between DC-SIGN binding capacity of milk and BSSL gene polymorphisms., Study Design: ELISA and PCR were used to study DC-SIGN binding properties and BSSL exon 11 size variation for human milk derived from 269 different mothers distributed over 4 geographical regions., Results: DC-SIGN binding properties were highly variable for milks derived from different mothers and between samplings from different geographical regions. Differences in DC-SIGN binding were correlated with a genetic polymorphism in BSSL which is related to the number of 11 amino acid repeats at the C-terminus of the protein., Conclusion: The observed variation in DC-SIGN binding properties among milk samples may have implications for the risk of mucosal transmission of pathogens during breastfeeding.
- Published
- 2011
- Full Text
- View/download PDF