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Simple Summary: Maize late wilt disease (LWD) is considered the most severe corn disease in Israel and Egypt and a significant threat in other countries. The utilization of disease-resistant maize cultivars is currently our best LWD control method. Here we evaluated the predictive ability of a rapid test to examine the susceptibility/tolerance of corn varieties to the disease. The fastest assay is based on the sensitivity of corn seeds to metabolites secreted by the pathogen. However, it is only reliable in detecting highly resistant or extremely susceptible maize varieties. To identify LWD immunity of mildly tolerant hybrid plants, a full-season pot assay in the open air, under field conditions, is needed. New detection methods, including aerial visible dehydration symptom monitoring and real-time PCR-based tracking of M. maydis DNA inside the plants' tissues, were evaluated here and proved to provide a great advantage. No complete overlap exists between the fungal DNA amount and the severity of symptoms. Such a correlation exists in high sensitivity or resistance cases but not in intermediate situations. Still, the valuation of the pathogen's establishment in asymptomatic corn hybrids is a powerful means to predict each maize variety's LWD immunity. Magnaporthiopsis maydis late wilt disease (LWD) in corn is considered to be the most severe in Israel and Egypt and poses a significant threat in other countries. Research efforts extending over a period of five decades led to the development of chemical, biological, agrotechnical, physical (solar disinfection) and other means for controlling late wilt disease. Today, some applications can reduce damage even in severe cases. However, cultivating disease-resistant maize varieties is the primary means for reducing the disease's impact. The current work uses a rapid (six days) laboratory seedling pathogenicity test and a full-season open encloser semi-field conditioned pots assay (101 days) to classify maize varieties according to their LWD resistance. To better evaluate differences between the cultivars, a real-time based molecular assay was applied to track the pathogen's presence in the plants' tissues, and visible light aerial imaging was used in parallel. The findings show that in cases of extreme sensitivity or tolerance (for example, in the highly susceptible Megaton cultivar (cv.) or the resistant Hatai cv.), a similarity in the results exists between the different methods. Thus, a reliable estimate of the varieties' sensitivity can be obtained in a seed assay without the need for a test carried out throughout an entire growing season. At the same time, in most situations of partial or reduced LWD sensitivity/resistance, there is no match between the various tests, and only the entire growing season can provide the most reliable results. Tracking the amount of M. maydis DNA in the plants' bodies is a precise, sensitive scientific tool of great importance for studying the development of the disease and the factors affecting it. Yet, no complete overlap exists between the fungal DNA amount and symptom severity. Such a correlation exists in high sensitivity or resistance cases but not in intermediate situations. Still, the valuation of the pathogen's establishment in asymptomatic corn hybrids can indicate the degree of LWD immunity and the chance of susceptibility development. [ABSTRACT FROM AUTHOR]

6-pentyl-α-pyrone (6-PP) is a powerful Magnaporthiopsis maydis antifungal compound, recently discovered when the potent growth medium of Trichoderma asperellum was analyzed. Despite its high potential in plate assay, it was not inspected for plant treatment prevention. Late wilt disease, caused by the fungus M. maydis, threatens commercial maize production in high-risk areas. Thus, the search for control options against the pathogen is one of the top priorities in Israel, Egypt, and other countries. Disease-resistant maize genotypes can reduce the damages. Yet, aggressive variants of the fungus can overcome host resistance. The current study aimed at inspecting T. asperellum and its secreted metabolite, pure 6-PP, against the pathogen in plants over a full growth period. First, adding T. asperellum directly to seeds with sowing provides significant protection to sprouts (up to 42 days) in a growth room, with more than two-fold growth promotion and reduced pathogen root infection (detected by real-time PCR). The same procedure applied in a commercial field was less beneficial in rescuing the plants' growth and yield. Still, it reduced the cobs' symptoms by 11% and resulted in nine-fold lower levels of the pathogen's DNA in the stem tissue. Second, the T. asperellum purified 6-PP compound (30 µg/seed) was used in seed coating and tested against the T. asperellum secretory metabolites' crude (diluted to 50%). At the season's end, these treatments improved plant biomass by 90–120% and cob weight by 60%. Moreover, the treatments significantly (p < 0.05) reduced the symptoms (up to 20%) and pathogen infection (94–98%). The current study's results reveal the potential of 6-PP as a new fungicide against M. maydis. Such a treatment may protect maize plants from other soil diseases. [ABSTRACT FROM AUTHOR]

In recent years, worldwide scientific efforts towards controlling maize late wilt disease (LWD) have focused on eco-friendly approaches that minimize the environmental impact and health risks. This disease is considered to be the most severe threat to maize fields in Israel and Egypt, and a major growth restraint in India, Spain, and Portugal. Today's most commonly used method for LWD control involving resistant maize genotypes is under constant risk from aggressive pathogen lines. Thus, this study's objectives were to evaluate the effect of crop rotation and avoiding tillage on restraining the disease. Such an agrotechnical approach will support the continuity of soil mycorrhiza networks, which antagonize the disease's causal agent, Magnaporthiopsis maydis. The method gained positive results in previous studies, but many knowledge gaps still need to be addressed. To this end, a dual-season study was conducted using the LWD hyper-susceptible maize hybrid, Megaton cv. The trials were performed in a greenhouse and in the field over full dual-growth seasons (wheat or clover as the winter crop followed by maize as the summer crop). In the greenhouse under LWD stress, the results clearly demonstrate the beneficial effect of maize crop rotation with clover and wheat on plant weight (1.4-fold), height (1.1–1.2-fold) and cob yield (1.8–2.4-fold), especially in the no-till soil. The clover-maize growth sequence excels in reducing disease impact (1.7-fold) and pathogen spread in the host tissues (3-fold). Even though the wheat-maize crop cycle was less effective, it still had better results than the commercial mycorrhizal preparation treatment and the uncultivated non-infected soil. The results were slightly different in the field. The clover-maize rotation also achieved the best growth promotion and disease restraint results (2.6-fold increase in healthy plants), but the maize rotation with wheat showed only minor efficiency. Interestingly, pre-cultivating the soil with clover had better results in no-till soil in both experiments. In contrast, the same procedure with wheat had a better impact when tillage was applied. It may be concluded that crop rotation and soil cultivation can be essential in reducing LWD, but other factors may affect this approach's benefits in commercial field growth. [ABSTRACT FROM AUTHOR]

Restricting maize late wilt disease (LWD), which is considered a major problem for commercial production in Israel, Egypt, Spain, and other countries, requires continuous efforts in developing novel strategies to study the pathogen and its host plant interactions, to monitor its spread and to contain its devastating impact. Despite recent years' encouraging success in developing agrotechnical, chemical, and biological control strategies, today's most environmentally-friendly, efficient, and cost-effective way to restrict the disease-causal agent, Magnaporthiopsis maydis, is to use highly resistant maize genotypes. The recent discovery of highly aggressive isolates of M. maydis that may threaten resistant maize cultivars is forcing researchers and farmers to increase programs for breeding resistant maize germlines and improve our ability to identify and nurture them. The current study offers remote sensing for evaluating maize cultivars' sensitivity to LWD based on the high-resolution, visible-channel, green–red vegetation index (GRVI), and thermal aerial imaging. A commercial field having a long history of M. maydis infestation was chosen to assess 12 fodder maize genotypes with different degrees of susceptibility to LWD. Visible and thermal aerial imaging during the growth season paralleled the disease progression evaluated by molecular monitoring of the pathogen DNA inside the host plants and the plants' growth parameters and yield at the end of the season. This remote technique to evaluate the cultivars' resistance/sensitivity to LWD may enable scanning and assessing a large group of plants simultaneously, discovering early symptomatic plants, and identifying hot spots in the field with intensive disease bursts. The method also allows to detect field-environmental structure and cultivation variations that may affect the disease severity. An examination of the nutritional values of highly resistant and highly susceptible genotypes – revealed that LWD manifested the most in a significant decrease in the plants' wet weight and less in changes in their nutritional values. No significant effect was found on nutritional values for the pathogen's latent presence in resistant maize plants. [ABSTRACT FROM AUTHOR]

Control of maize late wilt disease (LWD) has been at the forefront of research efforts since the discovery of the disease in the 1960s. The disease has become a major economic restraint in highly affected areas such as Egypt and Israel, and is of constant concern in other counties. LWD causes dehydration and collapsing at a late stage of maize cultivation, starting from the male flowering phase. The disease causal agent, Magnaporthiopsis maydis, is a seed- and soil-borne phytoparasitic fungus, penetrating the roots at sprouting, colonizing the vascular system without external symptoms, and spreading upwards in the xylem, eventually blocking the water supply to the plant's upperparts. Nowadays, the disease's control relies mostly on identifying and developing resistant maize cultivars. Still, host resistance can be limited because M. maydis undergoes pathogenic variations, and virulent strains can eventually overcome the host immunity. This alarming status is driving researchers to continue to seek other control methods. The current review will summarize the various strategies tested over the years to minimize the disease damage. These options include agricultural (crop rotation, cover crop, no-till, flooding the land before sowing, and balanced soil fertility), physical (solar heating), allelochemical, biological, and chemical interventions. Some of these methods have shown promising success, while others have contributed to our understanding of the disease development and the environmental and host-related factors that have shaped its outcome. The most updated global knowledge about LWD control will be presented, and knowledge gaps and future aims will be discussed. [ABSTRACT FROM AUTHOR]

Simple Summary: The maize (Zea mays L.) late wilt disease, caused by the fungus Magnaporthiopsis maydis, is considered the most severe threat to commercial maize production in Israel and Egypt. Various control strategies have been inspected over the years. The current scientific effort is focusing on eco-friendly approaches against the disease. The genus Trichoderma, a filamentous soil and plant root-associated fungi, is one of the essential biocontrol species, demonstrating over 60% of all the listed biocontrol agents used to reduce plant infectious diseases. They produce different enzymes and elicit defense responses in plants, playing a significant role in biotic and abiotic stress tolerance, hyphal growth, and plant growth promotion. Trichoderma asperellum was found to have biocontrol ability and protect crops against various plant pathogenic fungi, including the maize late wilt disease causal agent. This research aimed at isolating and identifying T. asperellum secondary metabolites with antifungal action against M. maydis. From T. asperellum growth medium, the 6-Pentyl-α-pyrone secondary metabolite was isolated and identified with high potent antifungal activity against M. maydis. This compound previously exhibited antifungal activities towards several plant pathogenic fungi. Achieving clean and identified T. asperellum active ingredient(s) secreted product(s) is the first step in revealing their commercial potential as new fungicides. Follow-up studies should test this component against the LWD pathogen in potted sprouts and the field. Late wilt disease (LWD) is a destructive vascular disease of maize (Zea mays L.) caused by the fungus Magnaporthiopsis maydis. Restricting the disease, which is a significant threat to commercial production in Israel, Egypt, Spain, India, and other countries, is an urgent need. In the past three years, we scanned nine Trichoderma spp. isolates as biological control candidates against M. maydis. Three of these isolates showed promising results. In vitro assays, seedlings pathogenicity trials, and field experiments all support the bio-control potential of these isolates (or their secretions). Here, a dedicated effort led to the isolation and identification of an active ingredient in the growth medium of Trichoderma asperellum (P1) with antifungal activity against M. maydis. This Trichoderma species is an endophyte isolated from LWD-susceptible maize seeds. From the chloroform extract of this fungal medium, we isolated a powerful (approx. 400 mg/L) active ingredient capable of fully inhibiting M. maydis growth. Additional purification using liquid chromatography–mass spectrometry (LC–MS) and gas chromatography–mass spectrometry (GC–MS) separation steps enabled identifying the active ingredient as 6-Pentyl-α-pyrone. This compound is a potential fungicide with high efficiency against the LWD causal agent. [ABSTRACT FROM AUTHOR]

Maize late wilt disease caused by the fungus Magnaporthiopsis maydis significantly damages crops in Israel and in other countries. Resistant maize cultivars are the preferred method for disease restraining. However, the pathogen populations of Spain and Egypt have varying aggressiveness, and virulent strains can overcome host resistance. In 2001 and from 2016 to -2019, 17 M. maydis strains were isolated from infected maize fields in Israel. The isolates' effects on seed germination, plant development, and disease symptoms severity were evaluated. The isolates from Israel display a diverse degree of aggressiveness that is not linked to their geographic distribution. The virulent strains are found in mixed populations, whereas less virulent M. maydis isolates exist. Aggressive strains harmed the development of plants and ears and caused severe wilting and death. In contrast, plants inoculated with less virulent strains exhibited only mild dehydration signs, and crop yield was similar to that of the non-infected control. Interestingly, different host cultivars can evoke specific virulence of M. maydis strains. Moreover, some pathogen strains significantly repress plant development, while the impact of other strains was evidenced by wilting symptoms. The current research further increases our understanding of the pathogen and our ability to control it. [Display omitted] • Israel is considered one of the world's most affected by maize late wilt disease. • This is the first work to describe Israel's pathogen dispersal and aggressiveness. • Virulent M. maydis strains scattered throughout the country in mixed populations. • Some strains mainly affect plant growth, while others disease symptoms. • Different host cultivars evoke specific M. maydi s strains' virulence. [ABSTRACT FROM AUTHOR]

The fungus Magnaporthiopsis maydis is a soil-borne, seed-borne vascular wilt pathogen that causes severe damage to sensitive Zea mays L. (maize) hybrids throughout Egypt, Israel, India, Spain, and other countries. It can undergo virulence variations and survive as spores, sclerotia, or mycelia on plant residues. Maize, Lupinus termis L. (lupine) and Gossypium hirsutum L. (cotton) are the only known hosts of M. maydis. Identification of new plant hosts that can assist in the survival of the pathogen is an essential step in restricting disease outbreak and spread. Here, by field survey and growth chamber pathogenicity test, accompanied by real-time PCR analysis, the presence of the fungal DNA inside the roots of cotton (Pima cv.) plants was confirmed in infested soil. Moreover, we identified M. maydis in Setaria viridis (green foxtail) and Citrullus lanatus (watermelon, Malali cv.). Infected watermelon sprouts had delayed emergence and development, were shorter, and had reduced root and shoot biomass. M. maydis infection also affected root biomass and phenological development of cotton plants but caused only mild symptoms in green foxtail. No M. maydis DNA was detected in Hordeum vulgare (barley, Noga cv.) and the plants showed no disease symptoms except for reduced shoot weight. These findings are an important step towards uncovering the host range and endophytic behavior of M. maydis, encouraging expanding this evaluation to other plant species. [ABSTRACT FROM AUTHOR]