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Eriocheir sinensis is an euryhaline crab found from East Asia to Europe and North America. This species can live in freshwater and seawater due to the unique physiological characteristics of their life cycle, which allows them to adapt and inhabit different habitats in a wide range of environments. Despite the wealth of studies focusing on adaptation mechanism of E. sinensis to specific environmental factors, the adaptation mechanisms to wild habitats with coexisting environmental factors are not well understood. In this study, we conducted a transcriptome analysis to investigate gene expression differences related to habitat adaptation of E. sinensis from two wild habitats with different environmental factors in the Han River, Korea. A total of 138,261 unigenes were analyzed, of which 228 were analyzed as differentially expressed genes (DEGs) between the two wild habitats. Among 228 DEGs, 110 DEGs were annotated against databases; most DEGs were involved in energy metabolism, immunity, and osmoregulation. Moreover, DEG enrichment analysis showed that upregulated genes were related to biosynthesis, metabolism, and immunity in an habitat representing relatively high salinity whereas downregulated genes were related to ion transport and hypoxia response in habitats with relatively low salinity and dissolved oxygen. The present findings can serve as foundation for future E. sinensis culture or conservation approaches in natural conditions. [ABSTRACT FROM AUTHOR]

Severe Helicobacter pylori-linked gastric disorders are especially prevalent in the East Asia region. The ability of H. pylori to cause different clinical outcomes is thought to be associated with unique sets of its genetic features. However, only few genetic features have been definitively linked to specific gastrointestinal pathologies. Genome heterogeneity of clinical H. pylori strains from patients with four different gastric disorders was studied to explore the population structure and molecular genomic features and their association with pathogenicity. Population analysis showed that 92.9% of the Shanghai H. pylori isolates were clustered in the East Asia group. Among 2,866 genes detected in all genomes, 1,146 genes formed the core genome, whereas 209 unique genes were detected in individual disease groups. The unique genes of peptic ulcer and gastric cancer groups represented the inorganic ion transport and metabolism function gene clusters. Sixteen virulence genes were detected with statistically different detection rates among the four disease groups. Furthermore, 127 clustered regularly interspaced short palindromic repeats were found with significantly different rates in the four disease groups. A total of 337 putative genomic islands were identified, and three genomic islands were individually found in more than 10% of strains. The genomic islands included several metabolism-associated genes and many genes with unknown function. In total, 88 sequence types were detected among the 112 Shanghai H. pylori isolates. Our study provides an essential milestone in the mapping of specific genomic features and their functions to identify factors needed to induce specific gastric disorders in H. pylori. [ABSTRACT FROM AUTHOR]

Severe Helicobacter pylori-linked gastric disorders are especially prevalent in the East Asia region. The ability of H. pylori to cause different clinical outcomes is thought to be associated with unique sets of its genetic features. However, only few genetic features have been definitively linked to specific gastrointestinal pathologies. Genome heterogeneity of clinical H. pylori strains from patients with four different gastric disorders was studied to explore the population structure and molecular genomic features and their association with pathogenicity. Population analysis showed that 92.9% of the Shanghai H. pylori isolates were clustered in the East Asia group. Among 2,866 genes detected in all genomes, 1,146 genes formed the core genome, whereas 209 unique genes were detected in individual disease groups. The unique genes of peptic ulcer and gastric cancer groups represented the inorganic ion transport and metabolism function gene clusters. Sixteen virulence genes were detected with statistically different detection rates among the four disease groups. Furthermore, 127 clustered regularly interspaced short palindromic repeats were found with significantly different rates in the four disease groups. A total of 337 putative genomic islands were identified, and three genomic islands were individually found in more than 10% of strains. The genomic islands included several metabolism-associated genes and many genes with unknown function. In total, 88 sequence types were detected among the 112 Shanghai H. pylori isolates. Our study provides an essential milestone in the mapping of specific genomic features and their functions to identify factors needed to induce specific gastric disorders in H. pylori. [ABSTRACT FROM AUTHOR]