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The number of species of crop plants in Central Europe increased constantly during the Bronze Age. The structure of the composition of cultivated plants was probably connected to the cultural contacts of human populations. During the Bronze Age (2300/2000–800 BC), the region of South Bohemia (Czech Republic) increasingly became the focus of long-distance trade and exchange networks with regions to the east and many other regions (the Eastern Alps, the Alpine Foreland, the central lowlands of the Czech Republic, Hungary and Western Slovakia). The aim of the paper is to examine archaeobotanical assemblages of charred plant remains to see if these changes within Bronze Age societies, and their spheres of interaction, are also recorded within their agricultural practices. In particular, the importance of specific individual crop species can be reflected in the study region in comparison with other individual regions of Central Europe. Humans in the region of South Bohemia had more connections with the Eastern Alps and the Alpine Foreland region during the Early and Middle Bronze Ages. Regarding the structure of crop species, the composition of sub/dominant crops in South Bohemia and the Eastern Alps and the Alpine Foreland had many similarities. The cultural trajectory of the human populations of the South Bohemian region changed substantially in the Late and Final Bronze Ages: intensive contacts are documented, primarily with the region of Central Bohemia. This is reflected in the composition of the sub/dominant crops in South Bohemia, which shows many similarities to the other regions of the Czech Republic. Changes in migration and exchange networks—in particular those that involved more formalised trade—are associated with a large number of innovations and specific goods and led to much wider levels of cultural and social integration within Bronze Age Europe than had been previously seen. [ABSTRACT FROM AUTHOR]