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Isthmohyla insolita and Exerodonta catracha are Honduran hylids of uncertain phylogenetic relationships. In this paper we present a phylogenetic analysis of mitochondrial and nuclear genes to study their relationships. Our results show that these species are, respectively, the sister taxon of and nested among the few available species of Plectrohyla, possibly the least studied genus of Hylini in terms of phylogenetic relationships. To solve the polyphyly of Exerodonta and Isthmohyla, both species are provisionally transferred to Plectrohyla, pending a broader phylogenetic study including all species in the genus. Consequently, Isthmohyla is restricted to a group of hylids endemic to the Isthmian highlands of Costa Rica and Panama in lower Central America. The inclusion of I. insolita in Plectrohyla implies another instance of independent evolution of terrestrial egg clutches in Hylini. [ABSTRACT FROM AUTHOR]

We report recent findings of Isthmohyla pictipes (Cope, 1875) in the Cordillera de Talamanca, Costa Rica, roughly two decades after it was last registered. We provide notes on microhabitat use, color variation, external morphology of adults and larvae, and geographic variation, and discuss some taxonomic characters employed to differentiate I. pictipes from I. tica (Starrett, 1966) and I. xanthosticta (Duellman, 1968). We also report fluorescence on the ventral surfaces of I. pictipes. Our findings are expected to shed light on the taxonomy of this species and should be useful in further population assessments and conservation plans.