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1. Systematic interpretation of molecular beacon polymerase chain reaction for identifying rpoB mutations in Mycobacterium tuberculosis isolates with mixed resistant and susceptible bacteria.

Author

Gomez DI, Fisher-Hoch SP, Bordt AS, Quitugua TN, Robledo J, Alvarez N, Correa N, McCormick JB, and Restrepo BI

Subjects
Animals, Colombia, DNA-Directed RNA Polymerases, Humans, Mexico, Microbial Sensitivity Tests methods, Mycobacterium tuberculosis genetics, Mycobacterium tuberculosis isolation & purification, Sensitivity and Specificity, Texas, Tuberculosis, Multidrug-Resistant microbiology, Antitubercular Agents pharmacology, Bacterial Proteins genetics, Mutation, Missense, Mycobacterium tuberculosis drug effects, Polymerase Chain Reaction methods, Rifampin pharmacology, Tuberculosis, Multidrug-Resistant diagnosis
Abstract

Detection of multidrug-resistant tuberculosis (MDR-TB), a frequent cause of treatment failure, takes 2 or more weeks to identify by culture. Rifampicin (RIF) resistance is a hallmark of MDR-TB, and detection of mutations in the rpoB gene of Mycobacterium tuberculosis using molecular beacon probes with real-time quantitative polymerase chain reaction (qPCR) is a novel approach that takes

Published
2010
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