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Since the rise of the Covid-19 pandemic, several researchers stated the possibility of a positive relationship between Covid-19 spread and climatic parameters. An ecological study in 12 Iranian cities using the report of daily deaths from Covid-19 (March to August 2020) and validated data on air pollutants, considering average concentrations in each city in the last year used to analyze the association between chronic exposure to air pollutants and the death rate from Covid-19 in Iran. Poisson regression models were used, with generalized additive models and adjustment variables. A significant increase of 2.7% (IC(95%) 2.6-4.4) was found in the mortality rate due to Covid-19 due to an increase of 1 μg/m 3 of NO 2 . The results suggest an association between Covid-19 mortality and NO 2 exposure. As a risk approximation associated with air pollution, more precise analysis is done. The results also show a good consistency with studies from other regions; this paper's results can be useful for the public health policymakers and decision-making to control the Covid-19 spread., (Copyright © 2021 Elsevier Inc. All rights reserved.)