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SDE-GC-MS was used to determine the content of flavor components in flue-cured tobacco leaves of suitable storage period in Chongqing tobacco-growing areas, and descriptive statistical analysis was carried out for the determined flavor components. Principal component analysis was used to screen the characteristic indexes of flavor components, and the difference significance analysis, multiple comparison and cluster analysis were carried out for the selected characteristic indexes. The results showed that there were significant differences in the content of 63 flavor components in tobacco leaves, and the variation coefficients of flavor components in upper and middle tobacco leaves were 12.12% ~ 59.97% and 10. 41% ~ 73.25% respectively. There were significant differences in the region, aged time, cultivar, and grade of tobacco leaves, and the influence degree of different factors were region > aged time > cultivar > grade, but there was almost no significant difference among the grades within the position. Characteristic index content of flavor components in upper tobacco leaves of Fengdu was higher, Fuling was the second, Wulong was the lowest. Characteristic index content of flavor components in middle tobacco leaves of Qianjiang was higher, Nanchuan was the second, Wulong was the lowest. The upper and middle tobacco leaves were divided into three categories. The first category of upper tobacco leaves includes Fengdu and Nanchuan, the second category includes Wulong, Peng-shui and Qianjiang, the third category is Fuling. The first category of middle tobacco leaves includes Fengdu, Nanchuan and Qianjiang, the second category includes Wulong and Fuling, and the third category includes Pengshui, with high significant differences between categories. [ABSTRACT FROM AUTHOR]