1. The associations of LPIN1 gene expression in adipose tissue with metabolic phenotypes in the Chinese population.
- Author
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Chang YC, Chang LY, Chang TJ, Jiang YD, Lee KC, Kuo SS, Lee WJ, and Chuang LM
- Subjects
- Adiponectin genetics, Adiponectin metabolism, Adult, Asian People genetics, Blood Glucose metabolism, Body Mass Index, China, Diabetes Mellitus, Type 2 metabolism, Enzyme-Linked Immunosorbent Assay, Female, Genetic Predisposition to Disease, Genetic Variation, Haplotypes, Humans, Insulin blood, Lipids blood, Male, Middle Aged, Nuclear Proteins metabolism, Obesity metabolism, Odds Ratio, PPAR gamma genetics, PPAR gamma metabolism, Phenotype, Phosphatidate Phosphatase, Polymorphism, Single Nucleotide genetics, RNA, Messenger genetics, RNA, Messenger metabolism, Reverse Transcriptase Polymerase Chain Reaction, Waist Circumference genetics, Adipose Tissue metabolism, Diabetes Mellitus, Type 2 genetics, Nuclear Proteins genetics, Obesity genetics
- Abstract
The LPIN1 gene, encoding lipin-1 protein, plays critical roles in adipocyte differentiation and lipid metabolism. This study aimed to analyze the association of LPIN1 mRNA levels in human adipose tissue with metabolic phenotypes. We also examined the association of LPIN1 genetic variation with type 2 diabetes and related metabolic phenotypes in the Chinese population. The relative LPIN1 mRNA levels were measured in abdominal visceral (VAT) and subcutaneous adipose tissue (SAT) obtained from 102 nondiabetic Chinese females. Seven single-nucleotide polymorphisms (SNPs) spanning from the 5'-upstream region to the 3'-end of the LPIN1 gene were genotyped in 1,520 Chinese (760 type 2 diabetic cases and 760 controls). LPIN1 mRNA levels in VAT were negatively correlated with BMI (r = -0.21, P = 0.03), body fat percentage (r = -0.22, P = 0.02), plasma triglycerides levels (r = -0.21, P = 0.03), and plasma leptin levels (r = -0.63, P = 0.0002). LPIN1 mRNA levels were positively correlated with PPARG and ADIPOQ mRNA levels in both VAT and SAT. No single SNP of the LPIN1 gene was associated with type 2 diabetes in our population. One rare haplotype showed a significant association with type 2 diabetes (odds ratio (OR), 4.35; 95% confidence interval, 1.86-11.75; P = 4 x 10(-4)). No SNP or haplotype of the LPIN1 gene was associated with quantitative metabolic traits in the nondiabetic subjects. The results confirmed the association of LPIN1 gene expression in adipose tissue with lower adiposity and favorable metabolic profiles in the Chinese population. However, the LPIN1 gene seemed not to be a major susceptibility gene for type 2 diabetes or related metabolic phenotypes in the Chinese population.
- Published
- 2010
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