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Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease discovered in China in 2009. The purpose of this study was to describe the spatiotemporal distribution of SFTS and to identify its environmental influencing factors and potential high-risk areas in Shandong Province, China. Methods: Data on the SFTS incidence from 2010 to 2021 were collected. Spatiotemporal scan statistics were used to identify the time and area of SFTS clustering. The maximum entropy (MaxEnt) model was used to analyse environmental influences and predict high-risk areas. Results: From 2010 to 2021, a total of 5705 cases of SFTS were reported in Shandong. The number of SFTS cases increased yearly, with a peak incidence from April to October each year. Spatiotemporal scan statistics showed the existence of one most likely cluster and two secondary likely clusters in Shandong. The most likely cluster was in the eastern region, from May to October 2021. The first secondary cluster was in the central region, from May to October 2021. The second secondary cluster was in the southeastern region, from May to September 2020. The MaxEnt model showed that the mean annual wind speed, NDVI, cattle density and annual cumulative precipitation were the key factors influencing the occurrence of SFTS. The predicted risk map showed that the area of high prevalence was 28,120 km2, accounting for 18.05% of the total area of the province. Conclusions: The spatiotemporal distribution of SFTS was heterogeneous and influenced by multidimensional environmental factors. This should be considered as a basis for delineating SFTS risk areas and developing SFTS prevention and control measures. [ABSTRACT FROM AUTHOR]

Background: Parents of children with cancer may experience persistent psychological distress and impaired physical health throughout their children's diagnosis and treatment. Objective: This study aimed to develop a mindful self-compassion program for parents of children with cancer and explore its effect. Methods: This pre-post-test study without a control group was conducted with 34 Chinese parents of children with cancer, using mixed methods. Participants received a 6-week internet-based self-help mindful self-compassion intervention. Self-compassion, post-traumatic stress symptoms, depression, and sleep quality were measured at baseline and at 10 weeks post-baseline. Semi-structured interviews were conducted with 9 completers within 10 days after the intervention was completed. Results: Significant differences were observed in self-compassion, re-experiencing, physical depressive symptoms, and sleep quality. Two participants reported feeling miserable or recalling distressing experiences, of which one withdrew from the study while the other completed the intervention. Conclusion: The program could improve self-compassion, re-experiencing, physical depressive symptoms, and sleep quality in parents of children with cancer, which demonstrated the feasibility of delivering a self-help mindful self-compassion intervention through the internet. Increasing retention rates in future studies merits further discussion. [ABSTRACT FROM AUTHOR]

Background: Digital health is important for sustainable health systems and universal health coverage. Since the outbreak of COVID-19, many countries, including China, have promoted the introduction of digital health in their medical services. Developing the next generation of physicians with digital health knowledge and skills is a prerequisite for maximizing the potential of digital health. Objective: We aimed to understand the perception of digital health among Chinese medical students, the current implementation of digital health education in China, and the urgent need of medical students. Methods: Our cross-sectional survey was conducted online and anonymously among current medical students in China. We used descriptive statistical analysis to examine participant demographic characteristics and the demand for digital health education. Additional analysis was conducted by grouping responses by current participation in a digital health course. Results: A total of 2122 valid responses were received from 467 medical schools. Most medical students had positive expectations that digital health will change the future of medicine. Compared with wearable devices (85.53%), telemedicine (84.16%), and medical big data (86.38%), fewer respondents believed in the benefits of clinical decision support systems (CDSS) (63.81%). Most respondents said they urgently needed digital health knowledge and skills, and the teaching method of practical training and internship (78.02%) was more popular than the traditional lecture (10.54%). However, only 41.45% wanted to learn about the ethical and legal issues surrounding digital health. Conclusions: Our study shows that the current needs of Chinese medical students for digital health education remain unmet. A national initiative on digital health education, is necessary and attention should be paid to digital health equity and education globally, focusing on CDSS and artificial intelligence. Ethics knowledge must also be included in medical curriculum. Students as Partners (SAP) is a promising approach for designing digital health courses. [ABSTRACT FROM AUTHOR]

In response to the social goal of 'carbon peak and carbon neutral' in the 14th Five-Year Plan of China, this article used Enrofloxacin (ENR), a common antibiotic, as a model compound to study the method of efficiently degrading pharmaceutical sludge and simultaneously producing Formic Acid (FA), hydrogen storage energy, in a sub-supercritical system. The Ni/SnO2 bimetallic catalyst, which was prepared by the equal volume impregnation method, was used for the liquid phase catalysis. As shown by the results, when the reaction temperature was 330°C, and the addition amount of H2O2 was 0.38 mL, the degradation rate of antibiotics could reach 99% after the reaction proceeded for 6 h. In terms of the resource utilization, the yield of FA could reach up to 32.44%. The resource utilization efficiency with Ni/SnO2 catalyst in sub-/supercritical reaction was about 2.5 times higher than that without catalyst. The kinetic reaction model was established to explore the reaction rate of the antibiotic degradation process. In addition, the Ea and the frequency factor of the reaction were 6455 J/mol and 5.78, respectively. As shown by characterization, the prepared Ni/SnO2 bimetallic catalyst had good activity and has already passed repeated stability experiments. In short, this method has broad application prospects in antibiotic catalysis and resource degradation. [ABSTRACT FROM AUTHOR]

Background: To investigate the association between clinical periodontal parameters of periodontitis, serum lipid metabolism markers and adipokines' levels in patients with obesity and periodontitis. Methods: A total of 112 patients admitted to Hospital of Xi'an Jiaotong University were included in this study. They were divided into normal body weight group (18.5 < body mass index, BMI < 25, n = 36), overweight group (25 ≤ BMI < 30, n = 38), and obesity group (BMI ≥ 30, n = 38) accordingly. The diagnosis of periodontitis was based on the newest international classification of periodontitis. Full-mouth clinical periodontal measurements included: plaque index, periodontal pocket depth, clinical attachment level, and bleeding on probing. Gingival crevicular fluid samples were analyzed for: Interleukin-1β, tumor necrosis factor-α, Interleukin-6 and C-reactive protein. Serum triglycerides, total cholesterol, low density lipoprotein cholesterol, high density lipoprotein cholesterol and glycosylated hemoglobin levels were measured. Visfatin, leptin, resistin, and adiponectin levels in serum were also measured. Results: The ratio of participants without periodontitis was significantly highest in normal weight group, and the proportion of severe periodontitis (stage III and IV) was highest in obesity group. The periodontal pocket depth, clinical attachment level, and the inflammatory cytokines in gingival crevicular fluid in obesity group and overweight group were higher than those in normal body weight group. The BMI and waist-to-hip ratio (WHR) were significantly positive correlated with periodontal pocket depth and clinical attachment level. Using a Multivariate logistic regression model, periodontitis correlates to BMI, WHR, serum levels of triglyceride, total cholesterol, low density lipoprotein, and adipokines such as visfatin, leptin, and resistin. Conclusions: Obesity is positively correlated with the aggravation of periodontitis. Obesity may aggravate the damage to periodontal tissue by regulating the secretion level of adipokines. [ABSTRACT FROM AUTHOR]

This study examines whether enterprises can obtain abnormal returns through philanthropic giving during public health emergencies, such as the COVID‐19 crisis, by using an event study approach to conduct an empirical analysis. The results show that if enterprises participate in philanthropic giving during the COVID‐19 crisis, they can obtain abnormal returns in the stock market. The extent of this market reaction is directly proportional to the level of philanthropic giving, and it is more significant for private enterprises, enterprises in regions that are more affected by the epidemic, enterprises in social responsibility sensitive industries, and enterprises with more timely donations. [ABSTRACT FROM AUTHOR]

As one of the paramount components of Asian monsoon and global atmospheric circulation system, the ISM (Indian summer monsoon) plays a vitally important role in the natural environment, ecological balance, and cultural development. Moreover, understanding the ISM could provide valuable insights into global hydrological, atmospheric, and carbon cycles. In recent decades, many ISM records based on different archives have been established within the Holocene, however, issues still remain: the TP (Tibetan Plateau) and the Indian subcontinent are often analyzed separately, despite the both are strongly influenced by the ISM, impeding comprehensive understanding of the monsoon and its object relationship with the East Asian summer monsoon (EASM). Here, covering the both regions, 65 published paleoclimate records within the Holocene have been collected, and conclusions are drawn as follow: (1) There are two evolution patterns of the ISM during the Holocene. The first pattern with the Holocene Optimum (HO) at ~10–6 ka BP, mainly distributing in the Arabian Sea and its surrounding areas, the Indian subcontinent, western central TP, northern TP, and southern China. The second pattern with the HO at ~8–3 ka BP, mainly distributing in the Bay of Bengal and its surrounding areas and southern TP. (2) The delay between the optimum of the second pattern and insolation maximum may be caused by the variation of high-latitude ice cover, although the underlying mechanism concerning the spatial distribution of the two patterns is still unclear. (3) The ISM and the EASM evolve synchronously, and share similar pattern divergence. Arguments over this issue mainly owe to the selected records originating from different patterns. This study makes up the gap in the pattern divergence of monsoon evolution, deepening the understanding of the ISM, even entire Asian monsoon system, and therefore is of great significance for future climate prediction. [ABSTRACT FROM AUTHOR]

The urban heat island intensity (UHII) is the temperature difference between urban areas and their rural surroundings. It is commonly attributed to changes in the underlying surface structure caused by urbanization. Air pollution caused by aerosol particles can affect the UHII through changing (1) the surface energy balance by the aerosol radiative effect (ARE) and (2) planetary-boundary-layer (PBL) stability and airflow intensity by modifying thermodynamic structure, which is referred to as the aerosol dynamic effect (ADE). By analyzing satellite data and ground-based observations collected from 2001 to 2010 at 35 cities in China and using the WRF-Chem model, we find that the impact of aerosols on UHII differs considerably: reducing the UHII in summer but increasing the UHII in winter. This seasonal contrast is proposed to be caused by the different strengths of the ARE and ADE between summer and winter. In summer, the ARE on UHII is dominant over the ADE, cooling down surface temperature more strongly in urban areas than in rural areas because of much higher aerosol loading, and offsets the urban heating, therefore weakening UHII. In winter, however, the ADE is more dominant, because aerosols stabilize the PBL more in the polluted condition, weakening the near-surface heat transport over urban areas in both vertical and horizontal directions. This means that the heat accumulated in urban areas is dispersed less effectively, and thus the UHII is enhanced. These findings shed new light on the impact of the interaction between urbanization-induced surface changes and air pollution on urban climate. [ABSTRACT FROM AUTHOR]

The voltage sag problem in the distribution network seriously affects the charging quality of electric vehicles. Therefore, evaluating the impact of voltage sag on electric vehicle charging is crucial for the development of electric vehicles. However, existing researches based on simulation models or independent power battery design and testing experiments cannot accurately and reliably reflect the charging interference of voltage sag on electric vehicles. This paper proposes for the first time the issue of voltage sag in China's distribution network affecting electric vehicle charging, and designs test cases to test the performance of electric vehicle charging under four kinds of sag parameters, two models of Brilliance BMW electric vehicles, two different temperatures, three brands of charging piles, and two vehicles charging simultaneously on the same power grid. In addition, this paper proposes a comprehensive solution for analyzing the impact of voltage sag on electric vehicle charging. Finally, an evaluation method based on the attention mechanism and BIGRU network has been developed to evaluate the adverse effect of voltage sag on the charging current of electric vehicles, with an accuracy of about 96.01% and a operating time of 0.4s. The superiority of the proposed method was further verified through comparative experiments with five mainstream methods. • Proposes a solution for analyzing the impact of power quality on vehicle charging. • Develop a novel method to evaluate the effect of voltage sag on vehicle charging. • Design cases based on real scenarios and the characteristics of voltage sag in China. • Develop a RBA-Net framework based on Bi-GRU network and attention mechanism. • A preprocessing method suitable for electric vehicle charging data is proposed. [ABSTRACT FROM AUTHOR]

Correction: BMC Medical Education (2023) 23:541 https://doi.org/10.1186/s12909-023-04407-w Following publication of the original article [[1]], the authors informed us that the order of the figures is wrong. The online version of the original article can be found at https://doi.org/10.1186/s12909-023-04407-w. [Extracted from the article]

Active development has become a necessity for societies to ensure ecological, social, and economical sustainability. This study aimed to investigate the coordinated development of the Hadaqi industrial corridor using GIS/RS technology and a system dynamics (SD) model for social development and the ecological environment. The results show that: (1) Population growth has certain impact, but predatory development of resources and unreasonable development practices is is the main cause for ecological environment deterioration. (2) It makes is the lowest GDP growth on inertia development model, the highest resource consumption and environmental pollution on economic development model, the rapid economic growth, the least pollution emissions and energy consumption on PRED model. (3) Hadaqi industrial corridor is a typical area of high intensity interaction between people and the nature in the current and a period of time in the future. PRED should be promoted step by step to achieve the coordinated development of social development and the ecological environment. [ABSTRACT FROM AUTHOR]

China is the largest producer and consumer of nonferrous metals in the world. China's consumption has posed a severe challenge to " peak carbon emissions and carbon neutrality " and "ecological civilization construction" goals of the 14th Five-Year Plan. Therefore, this article selects the five most typical nonferrous metal industries and analyses the energy structure through life cycle inventory ("LCI"). Then, this article uses the life cycle assessment ("LCA") to characterize, standardize, and weigh the environmental effects of the nonferrous metal industry. The contribution of the characteristic value is mainly from high-energy-consuming processes such as electrolysis and smelting. High-energy-consuming proportions are approximately 80% and 10%, respectively. Of the results of the standardized and weighted models, the most important environmental impact is "global warming". Based on the LCA results, we propose energy-saving and emission reduction methods. The LCA model would support decisions aimed at achieving the "peak carbon emissions and carbon neutrality" and "reducing pollution and carbon and coordinating governance strategic plan". If these countermeasures are effectively implemented, the emission reduction effect of environmental pollution will reach 87.94%–99.12%. According to the economic evaluation, implementing an emission reduction measure could create an economic value of RMB 1.35 × 1013 per year. [Display omitted] • Evaluate environmental impacts of nonferrous metal industry LCA model. • Countermeasures of emission reduction in technical and political perspective. • Evaluate economics of energy saving and emission reduction measures. [ABSTRACT FROM AUTHOR]

The article offers information on online medical clerkships in China in a single institution since the outbreak of COVID-19. Topics discussed include groupings made on the physicians according to their subspecialties, teaching contents in each online clerkship, and lessons learned from the initiative.

Land use and land cover change (LUCC) determines landscape patterns and affects the ability of ecosystems to provide the services and biodiversity on which humans ultimately depend. The Honghe National Nature Reserve is a biodiversity-rich area in the Jiansanjiang region of northeast China. In this study, the LUCC and landscape patterns were compared between the reserve and the Jiansanjiang region. With remote sensing (RS) and geographic information system (GIS) procedures, satellite images from 1989, 2001 and 2010 were used to calculate dynamics of LUCC amplitudes, landscape pattern index, transition matrix. The influence of LUCC on the wetland landscape of the nature reserve was discussed in the context of broader LUCC analyses. The results showed that land uses in the studied regions changed remarkedly over the time period; swamp area declined significantly as arable land increased. The ecological landscape index showed that distinct landscape patches were small, scattered and highly fragmented, moving toward a more monotonous landscape. Anthropogenic activities such as farming directly decreased the wetland landscape diversity. Furthermore, anthropogenic factors diminished wetland areas indirectly through climatic factors, namely air temperature and precipitation levels. National and regional policies on agriculture and water use have been highly influential, and the Honghe National Nature Reserve is highly susceptible to land use changes in the surrounding Jiansanjiang region. [ABSTRACT FROM AUTHOR]

The rapidly increasing penetration of wind power into sending-side systems makes the wind power curtailment problem more severe. Enhancing the total transfer capability (TTC) of the transmission channel allows more wind power to be delivered to the load center; therefore, the curtailed wind power can be reduced. In this paper, a new method is proposed to enhance TTC, which works by optimizing the day-ahead thermal generation schedules. First, the impact of thermal generation plant/unit commitment on TTC is analyzed. Based on this, the day-ahead thermal generation scheduling rules to enhance TTC are proposed herein, and the corresponding optimization models are established and solved. Then, the optimal day-ahead thermal generation scheduling method to enhance TTC is formed. The proposed method was validated on the large-scale wind power base sending-side system in Gansu Province in China; the results indicate that the proposed method can significantly enhance TTC, and therefore, reduce the curtailed wind power. [ABSTRACT FROM AUTHOR]

Black locust (Robinia pseudoacacia L.) is an economically and ecologically important tree species which is used for pillar construction, honey production and soil improvement. More EST-SSR (Expressed sequence tag simple sequence repeat) markers of black locust can be used as a complement and improvement of Genomic-SSR markers for the identification of the function of gene and the construction of genetic map. Additionally, currently there is no simple method for identifying black locust cultivars. In this study, we obtained 2702 unigenes from 3095 expressed sequence tags (ESTs) from the National Center of Biotechnology Information (NCBI) database to identify simple sequence repeats (SSRs) in R. pseudoacacia samples. A total of 170 SSR loci were found to be distributed in 162 non-redundant sequences with a frequency of 6.29%. Dinucleotide repeats were the most predominant types among microsatellites (62.35%), followed by tri-nucleotide repeats (25.88%); the remaining SSRs accounted for less than 12%. The repeat motifs AG/TC (29.25%) and CT/GA (29.25%) were the most abundant among dinucleotides, and AAT/TTA (15.91%) was the most common among tri-nucleotides. A total of 62 primer pairs were designed to screen polymorphic and stable SSR loci. The resulting 25 EST-SSR markers capable of amplifying polymorphic, stable, and repeatable products. Eight newly developed EST-SSR markers and four published SSR markers were selected for DNA fingerprinting and genetic diversity analysis of the 123 main R. pseudoacacia cultivars in China. The 12 SSR loci amplified 102 alleles, with an average number of alleles per locus of 8.5 (range 4–15). The average polymorphism information content at the 12 SSR loci for the 123 cultivars was 0.670 (range 0.427–0.881). The 123 cultivars clustered into six main groups based on similarity coefficients, with most cultivars in one subgroup. Fingerprinting was performed using eight SSR markers; 110 black locust cultivars were distinguished. The results of this study increase the availability of EST-SSR markers in black locust and make it a simple method for checking the collection, the certification, and the correct attribution of clones and cultivars. [ABSTRACT FROM AUTHOR]

Background The aim of the present study was to assess the quality of diabetes care and characteristics of the healthcare system in underdeveloped rural mountain areas of western China. Methods Questionnaires were used to collect data from 288 diabetic patients with a multistage cluster sampling method in Zhongjiang County (Sichuan Province) between October 2009 and April 2010. Sixty-two village clinics, 23 town health centers, and a county central hospital were included to assess the availability of diabetes-related medical resources, in addition to diabetes-related medical insurance, reimbursement policies, and manpower. Results Of 288 patients, 38.2 % monitored their blood glucose regularly. Targets for fasting blood glucose (≤7 mmol/L) and blood pressure (≤130/80 mmHg) were achieved by 7.6 % and 9.7 % of patients, respectively. On average, each patient paid US$120 out of pocket annually for out-patient diabetes care, with a maximum US$86 reimbursed. The county central hospital was the only healthcare facility in the county that could provide all essential diabetes-related drugs and process-of-care measures and tests, except measures of HbA1c and the urinary albumin: creatinine ratio. Insulin was not available at village clinics, and only 29 % of village clinics had glucometers. 'Certified' doctors were not available to provide primary care in village clinics. Conclusions The quality of diabetes care was quite poor in underdeveloped rural mountain areas of western China. Recommendations for further intervention research to improve diabetes healthcare include increasing investment in medical infrastructure, improving the availability of essential drugs and process measures, organizing regular diabetes patient education, and recruiting village doctors. [ABSTRACT FROM AUTHOR]

The concentrations and compositions of organophosphorus flame retardants (OPFRs) in various heat preservation materials from different brands and types in China were investigated, as well as their OPFR burdens and emission amounts. The average concentrations of ΣOPFRs were 41.4 ± 10.2, (7.1 ± 4.0) × 104, and 56.3 ± 19.3 μg/g in phenolic foams (PF), polyurethane foams (PUR), and extruded polystyrene boards (XPS), respectively. OPFRs in the PUR materials were ∼3 orders of magnitude higher than those in the other two materials, suggesting that organophosphate esters were added as flame retardants (FRs) in the PUR materials. Obvious variations in the concentrations and compositions of OPFRs were discovered in those heat preservation materials due to their material types, brands, and fire-ratings, as well as contaminations. TCIPP was the most dominant OPFR with a range from 22.3 (in PF) to 6.73 × 104 (in PUR) μg/g, which emphasized that TCIPP was the most commonly used flame retardant additives in China. Based on the OPFR concentrations, OPFR emission rates, and application amounts of heat preservation materials, we calculated the total burdens of OPFRs in those materials and their emission amounts in China. The potential total burden of OPFRs in the completed new buildings of China in 2017 was estimated to be ∼(2.37 ± 1.11) × 104 t, while the total emission of OPFRs from those new materials was ∼3.19 ± 1.65 t/y. As significant sources of OPFRs, the heat preservation materials used in exterior or interior walls may pose potential health risks to humans. Image 1 • Significantly high levels of TCIPP were found in polyurethane insulation materials. • OPFR burden in insulation materials of new buildings of China was ∼23.7 kt in 2017. • The OPFR emission amount from newly used insulation materials was ∼3.19 t in 2017. • The wall insulation materials are the significant sources of OPFRs. [ABSTRACT FROM AUTHOR]

[Display omitted] • Construction techniques of the tunnel in water-rich sand fine strata were provided. • Dewatering and reinforcing tunnel face will increase the pressure monitored of lining. • Deformation characteristics of support of three excavation methods were determined. The surrounding rock of the Taoshuping tunnel is fine silty sand in a plastic flow state after encountering water, which leads to inferior stability of the surrounding rock and extremely high construction risk. Therefore, the Taoshuping tunnel project is considered to be a rare worldwide problem in China. This article aims to review the engineering problems, construction techniques, and technical points faced in Taoshuping tunnel's construction, as well as reveal the evolution characteristics of surrounding rock pressure and evaluate structural safety. First, through the analysis of the characteristics and difficulties of the project, the combined dewatering scheme of vacuum wellpoint dewatering and collecting wells was formulated. Three kinds of tunnel excavation methods were developed: the double-heading at bottom method, both-side heading method with 9 steps, and ADECO-RS (Analysis of COntrolled DEformation in Rocks and Soils). Second, the variation law of the water content for the surrounding rock during excavation was studied through experiments. The water content that can ensure the stability of the surrounding rock is approximately 6.5%. Then, the evolution characteristics of the surrounding rock pressure of the first lining, the liner contact pressure, and the axial force of the reinforcement during construction were monitored and analyzed. The safety of the liner was evaluated by the pressure ratio of the liner and the strength-based safety factor. Finally, the deformation characteristics of the surrounding rock in the context of different excavation methods were investigated by numerical simulation. The lessons learned from the Taoshuping tunnel can provide insights for the increasing number of similar projects. [ABSTRACT FROM AUTHOR]

In light of China's progressively aging population, family care with the "warmth of affection" has always been an irreplaceable form of care that meets the wishes of the majority of disabled elderly to enjoy their twilight years comfortably. Data from a follow-up survey on the influencing factors of the Chinese Longitudinal Healthy Longevity Survey from 2018 and the Anderson Model was used as a theoretical framework to analyze the influencing factors using a binary logistic regression model. Children were the main providers of care services among disabled elderly in family care. Family care for disabled elderly was influenced by the combined effects of age (P < 0.01), gender (P < 0.05), marital status (P < 0.01), number of children (P < 0.01), housing ownership (P < 0.05), primary carer preference (P < 0.05), and self-assessed health (P < 0.01) were jointly affected. As a result, it's critical to set up a strong social support network that is focused on family caregiving and includes tailored interventions based on the requirements of disabled elderly and family caregivers., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Zhang, Wang. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Background: Point-of-care Testing (POCT) glycosylated hemoglobin (HbA1c) is a convenient, cheap, effective and accessible screening method for type 2 diabetes in rural areas and community settings that is widely used in the European region and Japan, but not yet widespread in China. The study is the first to evaluate the cost-effectiveness of POCT HbA1c, fasting capillary glucose (FCG), and venous blood HbA1c to screen for type 2 diabetes in urban and rural areas of China, and to identify the best socio-economically beneficial screening strategy., Methods: Based on urban and rural areas in China, economic models for type 2 diabetes screening were constructed from a social perspective. The subjects of this study were adults aged 18-80 years with undiagnosed type 2 diabetes. Three screening strategies were established for venous blood HbA1c, FCG and POCT HbA1c, and cost-effectiveness analysis was performed by Markov models. One-way sensitivity analysis and probabilistic sensitivity analysis were performed on all parameters of the model to verify the stability of the results., Results: Compared with FCG, POCT HbA1c was cost-effective with an incremental cost-utility ratio (ICUR) of $500.06/quality-adjusted life year (QALY) in urban areas and an ICUR of $185.10/QALY in rural areas, within the willingness-to-pay threshold (WTP = $37,653). POCT HbA1c was cost-effective with lower cost and higher utility compared with venous blood HbA1c in both urban and rural areas. In the comparison of venous blood HbA1c and FCG, venous blood HbA1c was cost-effective (ICUR = $20,833/QALY) in urban areas but not in rural areas (ICUR = $41,858/QALY). Sensitivity analyses showed that the results of the study were stable and credible., Conclusions: POCT HbA1c was cost-effective for type 2 diabetes screening in both urban and rural areas of China, which could be considered for future clinical practice in China. Factors such as geographic location, local financial situation and resident compliance needed to be considered when making the choice of venous blood HbA1c or FCG., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Shao, Xie, Wang, Gao, Hu and Zhang.)

Background: This study aimed to adopt the conventional signal detection methods to explore a new way of risk identification and to mine important drug risks from the perspective of big data based on Zhenjiang Adverse Event Reporting System (ZAERS)., Research Design and Methods: Data were extracted from ZAERS database between 2012 and 2022. The risks of all the reported drug event combinations were identified at the preferred term level and the standardized MedDRA query level using disproportionality analysis. Then, we conducted signal assessment according to the descriptions of drug labels., Results: In total 41,473 ADE were reported and there were 12 risky signals. Signal assessment indicates the suspected causal associations in clindamycin-taste and smell disorders, valsartan-hepatic enzyme increased and valsartan-edema peripheral; the specific manifestations of allergic reactions triggered by clindamycin, cefotaxime, cefazodime, ShexiangZhuanggu plaster, ShexiangZhuifeng plaster, and Yanhuning need to be refined in drug labels. In addition, the drug labels of NiuHuangShangQing tablet/capsule, Fuyanxiao capsule, and BiYanLing tablet should be improved., Conclusions: In this study, we attempted a new way to find potential drug risks using small spontaneous reporting data. Our findings also suggested the need for more precise identification of allergic risks and the improvement of traditional Chinese medicine labels.

Background: Traditional microbiological detection methods used to detect pulmonary infections in people living with HIV (PLHIV) are usually time-consuming and have low sensitivity, leading to delayed treatment. We aimed to evaluate the diagnostic value of metagenomics next-generation sequencing (mNGS) for microbial diagnosis of suspected pulmonary infections in PLHIV., Methods: We retrospectively analyzed PLHIV who were hospitalized due to suspected pulmonary infections at the sixth people hospital of Zhengzhou from November 1, 2021 to June 30, 2022. Bronchoalveolar lavage fluid (BALF) samples of PLHIV were collected and subjected to routine microbiological examination and mNGS detection. The diagnostic performance of the two methods was compared to evaluate the diagnostic value of mNGS for unknown pathogens., Results: This study included a total of 36 PLHIV with suspected pulmonary infections, of which 31 were male. The reporting period of mNGS is significantly shorter than that of CMTs. The mNGS positive rate of BALF samples in PLHIV was 83.33%, which was significantly higher than that of smear and culture (44.4%, P<0.001). In addition, 11 patients showed consistent results between the two methods. Futhermore, mNGS showed excellent performance in identifying multi-infections in PLHIV, and 27 pathogens were detected in the BALF of 30 PLHIV by mNGS, among which 15 PLHIV were found to have multiple microbial infections (at least 3 pathogens). Pneumocystis jirovecii, human herpesvirus type 5, and human herpesvirus type 4 were the most common pathogen types., Conclusions: For PLHIV with suspected pulmonary infections, mNGS is capable of rapidly and accurately identifying the pathogen causing the pulmonary infection, which contributes to implement timely and accurate anti-infective treatment., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Hou, Wang, Yuan, Zhang, Luo, Xin and Zhao.)

Background: The psychological status of Chinese postgraduate students majoring in stomatology after the COVID-19 restrictions still remains unclear. The objective of this study is to evaluate the mental status through a cross-sectional survey and gather related theoretical evidence for psychological intervention on postgraduate students majoring in stomatology., Methods: An online survey was administered, and subjective well-being, anxiety, stress and depression symptoms were assessed using the 5-item World Health Organization Well-Being Index (WHO-5), item Generalized Anxiety Disorder Scale (GAD-7), 10-item Perceived Stress Scale (PSS-10), and Patient Health Questionnaire-9 (PHQ-9), respectively, wherein suicidal ideation and sleep-related problems were measured with PHQ-9 and Insomnia Severity Index (ISI)., Results: A total of 208 participants who completed one questionnaire were considered as valid. It was found that female respondents generally exhibited significantly higher levels of PSS-10, PHQ-9, and GAD-7 scores and shorter physical activity hours than male students. Students from rural areas demonstrated significantly higher levels of PHQ-9, suicidal ideation, and less portion of good or fair family economic support. Additionally, individuals from only-child families reported increased levels of activity hours (1.78 ± 2.07, p  = 0.045) and a higher portion (55.10%, p  = 0.007) of having clear future plan as compared with multiple-child families. The risk factors for anxiety symptoms (GAD-7 score) were higher scores of PSS-10 ( OR  = 1.15, 95% CI = 1.09-1.22), PHQ-9 ( OR  = 1.35, 95% CI = 1.22-1.49), and ISI-7 ( OR  = 1.14, 95% CI = 1.06-1.23), while owning a clear graduation plan was the protective factor ( OR  = 0.55, 95% CI = 0.31-0.98). Moreover, the risk factors for depressive symptoms (PHQ-9) included PSS-10 ( OR  = 1.10, 95% CI = 1.04-1.16), GAD-7 ( OR  = 1.38, 95% CI = 1.25-1.52), suicidal ideation ( OR  = 5.66, 95% CI = 3.37-9.51), and ISI-7 ( OR  = 1.17, 95% CI = 1.09-1.25). Approximately 98.08% of Chinese postgraduates studying stomatology reported experiencing at least moderate stress after the COVID-19 restrictions., Conclusion: Within the limitations of this study, senior students were more inclined to stress, while anxiety symptoms were related to severer levels of stress, depression, and insomnia. Depressive symptoms were associated with higher levels of stress, anxiety, insomnia, suicidal ideation, and lower levels of self-reported well-being. Thus, psychological interventions for postgraduates should be timely and appropriately implemented by strengthening well-being, reasonably planning for the future, and good physique, thereby mitigating the psychological issues after COVID-19 restrictions., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Zhang, Jia, MuLaTiHaJi, Mi, Mei, Sun, Shi, Zhang, Zhang, Zou, Niu and Dong.)

Background: Hemorrhagic fever with renal syndrome (HFRS) signals a recurring risk in Eurasia in recent years owing to its continued rise in case notifications and the extension of geographical distribution. This study was undertaken to investigate the spatiotemporal drivers and incidence heterogeneity of HFRS transmission in Shandong Province., Methods: The epidemiological data for HFRS, meteorological data and socioeconomic data were obtained from China Information System for Disease Control and Prevention, China Meteorological Data Sharing Service System, and Shandong Statistical Yearbook, respectively. The spatial-temporal multicomponent model was employed to analyze the values of spatial-temporal components and the heterogeneity of HFRS transmission across distinct regions., Results: The total effect values of the autoregressive, epidemic, and endemic components were 0.451, 0.187, and 0.033, respectively, exhibiting significant heterogeneity across various cities. This suggested a pivotal role of the autoregressive component in propelling HFRS transmission in Shandong Province. The epidemic component of Qingdao, Weifang, Yantai, Weihai, and Jining declined sharply at the onset of 2020. The random effect identified distinct incidence levels associated with Qingdao and Weifang, signifying regional variations in HFRS occurrence., Conclusions: The autoregressive component emerged as a significant driver in the transmission of HFRS in Shandong Province. Targeted preventive measures should be strategically implemented across various regions, taking into account the predominant component influencing the epidemic., (© 2024. The Author(s).)

China is the largest industrial and pharmaceutical country in the world. The pharmaceutical industry, being a highly polluting sector, is the primary target of environmental regulation in the industry. The rapid development of pharmaceutical industry has posed a severe challenge to the environmental protection strategy of "carbon reduction and carbon neutrality" and the goal of "synergizing the reduction of pollution and carbon emissions" in China's "14th Five-Year Plan". Therefore, this paper starts from the whole industry, takes the life cycle of the whole production process of the pharmaceutical industry as the guidance, and selects a pharmaceutical company in Tianjin as the research object. Then using Life Cycle Assessment (LCA) to Characterization, Standardization, and Weighting the environmental impact of the waste gas treatment process before and after improvement based on waste gas emission characteristics from the pharmaceutical factory. LCA results show that GWP and AP are the most important environmental impact types, which account for >85 % of the total characterization value. I and II - Chemical Pharmaceutical Stage is the critical life cycle stage, accounting for over 80 % of the total characteristic values. This research proposes emission reduction countermeasures based on LCA results and simulates Emission reduction scenarios and economic evolution. If effectively implementing emission reduction countermeasures, reducing the environmental characterization value by 80 to 90 %, and generating economic benefit of 2.66 × 10 8 RMB/year. This research could guide improvement plans and emission reduction countermeasures of waste gas treatment in the pharmaceutical industry, which realizes collaborative management about efficient reduction of pollution and carbon and generates significant environmental, technological, economic, and social benefits., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

COVID-19 is an acute respiratory infectious disease caused by SARS-CoV-2. It was first reported in Wuhan, China in December 2019 and rapidly spread globally in early 2020, triggering a global pandemic. In December 2022, China adjusted the dynamic COVID-zero strategy that lasted for three years. The number of positive cases in China increased rapidly in the short term. Weihai was also affected during this period. We conducted genomic surveillance of SARS-CoV-2 variants in Weihai during this period, hoping to understand the changes in the genomic characteristics of SARS-CoV-2 before and after the adjustment of the epidemic policy. In this study,we collected SARS-CoV-2 positive samples from March 2022 to March 2023 in Weihai and performed SARS-CoV-2 whole genome sequencing on these samples using next-generation sequencing technology. we obtained a total of 704 SARS-CoV-2 whole genome sequences, and selected 581 high-quality sequences for further analysis. The analysis results showed that from March 2022 to November 2022, before the adjustment of epidemic policy, the COVID-19 cases in Weihai were mainly from four local clusters,which were caused by four variants, including BA.2,BA.1.1,P.1.15 and BA.5.2.1. Phylogenetic analysis showed that: In the same cluster,the sequences between each other were highly homologous, and the whole genome sequence were almost identical. After December 2022, the epidemic policy was adjusted, BF.7 and BA.5.2 became the dominant variants in Weihai, consistent with the main domestic strains in China during the same period. Phylodynamic analysis showed that BF.7 and BA.5.2 had a large amount of genetic diversities in December, and the effective population size of BF.7 and BA.5.2 also showed explosive growth in December. In conclusion, we reported the composition and dynamic trend of SARS-CoV-2 variants in Weihai from March 2022 to March 2023. We found that there have been significant changes in the variants and expansion patterns of SARS-CoV-2 before and after the adjustment of epidemic policies. But the dominant variants in Weihai were the same as the SARS-CoV-2 variants circulating globally at the same time and we found no persistently dominant variants or new lineages during this period., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Li, Zhang, Zhang, Sui, Qu, Wang, Miao and Li.)

Objective: To analyze the clinical features and genetic variant in a patient with Usher syndrome., Methods: Whole exome sequencing was carried out for the patient. Suspected variants were validated by Sanger sequencing of her parents and fetus., Results: The proband was found to harbor compound heterozygous variants c.17&#95;18insA (p.Tyr6Ter*) and c.4095&#95;4096insA (p.Arg1366Lys fs*38) of the PCDH15 gene (NM&#95;033056), which were respectively inherited from her father and mother. The same variants were not detected in 100 healthy controls. Based on the guidelines of the American Society of Medical Genetics and Genomics, both variants were predicted to be pathogenic (PVS1+PM2+PP4). By prenatal diagnosis, her fetus was found to carry the c.4095&#95;4096insA variant. After birth, the child has passed neonatal hearing screening test, and no abnormal auditory and visual function was found after the first year., Conclusion: The compound heterozygous variants c.17&#95;18insA (p.Tyr6Ter*) and c.4095&#95;4096insA (p.Arg1366Lys fs*38) of the PCDH15 gene probably underlay the Usher syndrome is this proband.

Objective: To explore the genetic basis for a Chinese pedigree affected with dyschromatosis symmetrica hereditaria (DSH)., Methods: PCR and Sanger sequencing were carried out for the proband, and suspected variant was validated by Sanger sequencing in the pedigree., Results: The proband was found to harbor a novel variant of c.1352delA (p.N451Mfs*13) of the ADAR (NM&#95;001111) gene. The same variant was found in her affected mother and sister, but not in her unaffected father, uncle, and 100 healthy individual., Conclusion: The novel variant of the ADAR gene probably underlay the pathogenesis of DSH in this pedigree.

Rationale: Acute respiratory distress syndrome (ARDS) is one of the major reasons for ventilation and intubation management of COVID-19 patients but there is no noninvasive imaging monitoring protocol for ARDS. In this study, we aimed to develop a noninvasive ARDS monitoring protocol based on traditional quantitative and radiomics approaches from chest CT. Methods: Patients diagnosed with COVID-19 from Jan 20, 2020 to Mar 31, 2020 were enrolled in this study. Quantitative and radiomics data were extracted from automatically segmented regions of interest (ROIs) of infection regions in the lungs. ARDS existence was measured by Pa02/Fi02 <300 in artery blood samples. Three different models were constructed by using the traditional quantitative imaging metrics, radiomics features and their combinations, respectively. Receiver operating characteristic (ROC) curve analysis was used to assess the effectiveness of the models. Decision curve analysis (DCA) was used to test the clinical value of the proposed model. Results: The proposed models were constructed using 352 CT images from 86 patients. The median age was 49, and the male proportion was 61.9%. The training dataset and the validation dataset were generated by randomly sampling the patients with a 2:1 ratio. Chi-squared test showed that there was no significant difference in baseline of the enrolled patients between the training and validation datasets. The areas under the ROC curve (AUCs) of the traditional quantitative model, radiomics model and combined model in the validation dataset was 0.91, 0.91 and 0.94, respectively. Accordingly, the sensitivities were 0.55, 0.82 and 0.58, while the specificities were 0.97, 0.86 and 0.98. The DCA curve showed that when threshold probability for a doctor or patients is within a range of 0 to 0.83, the combined model adds more net benefit than "treat all" or "treat none" strategies, while the traditional quantitative model and radiomics model could add benefit in all threshold probability. Conclusions: It is feasible to monitor ARDS from CT images using radiomics or traditional quantitative analysis in COVID-19. The radiomics model seems to be the most practical one for possible clinical use. Multi-center validation with a larger number of samples is recommended in the future., Competing Interests: Competing Interests: The authors have declared that no competing interest exists., (© The author(s).)

Lactobacillus spp., as probiotics, have shown efficacy in the inhibition of pathogenic bacteria in the intestinal tract. In this study, we investigated the antibacterial activity of Lactobacillus casei NA-2, which was isolated from northeast sauerkraut in China. The results of co-culture suggested that L. casei NA-2 could inhibit the growth of Bacillus cereus, Staphylococcus aureus, Salmonella typhimurium and Escherichia coli O157:H7. Moreover, L. casei NA-2 could adhere to the four pathogenic bacteria potentially associated with its exopolysaccharide (EPS). EPS from L. casei NA-2 was then isolated and its activity determined. The results showed that EPS inhibited the biofilms of B. cereus, S. aureus, S. typhimurium and E. coli O157:H7, with the highest inhibition ratios of 95.5% ± 0.1%, 30.2% ± 3.3%, 14.3% ± 0.6%, and 16.9% ± 5.4%, respectively. Moreover, EPS was able to disperse B. cereus, S. aureus, S. typhimurium and E. coli O157:H7 by 94.1% ± 1.2%, 31.8% ± 8.6%, 40.8% ± 3.3% and 49.6% ± 3.8%, respectively. Results showed that EPS from L. casei NA-2 have potential antibacterial properties by inhibiting biofilm formation and dispersing pathogenic bacteria. In conclusion, the antibiofilm property of the EPS on the surface of L. casei NA-2 is one of the possible reasons for antibacterial activity of L. casei NA-2.

We investigated the concentrations, distributions, and sources of PAHs in the air and soils of Dalian, China, as well as their air-soil exchange trends. Total concentrations of PAHs in the air ranged from 6.37 to 124 ng/m 3 with an average of 23.1 ± 26.6 ng/m 3 , while Σ 15 PAHs in the soils ranged from 42.8 to 28600 ng/g with an average of 2580 ± 5730 ng/g. Significant spatial distribution of PAHs was discovered in the soils with a clear urban-suburban-rural decreasing gradient, suggesting urban area is more contaminated by PAHs due to frequent and intensive human activities. However, high PAH concentrations were also discovered in the air from several rural sites, implying some PAH sources have shifted from urban to suburban or rural areas. Source apportionment indicated that major sources of PAHs in the urban and suburban soils were traffic emission/oil spill and coal combustion respectively, whereas major sources in the rural soils were diverse. Air-soil partitioning result suggested that 3-ring PAHs were mostly volatilized from soil, 6-ring PAHs were deposited into soil, while the trends of 4∼5-ring PAHs varied with sampling site. Fluoranthene, Pyrene, and Chrysene were mostly discovered to be volatilized in the urban and suburban areas, but equilibrium or deposited in the rural area, indicating a potential urban-to-rural transport of PAHs. The atmospheric transport and source shift of PAHs from urban to rural areas highlighted the importance of PAH source control on a regional scale., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

We investigated the concentrations and seasonal variations of organophosphate esters (OPEs) in the gas phase and PM 2.5 (particulate matter with an aerodynamic diameter <2.5 μm) in an urban area of Dalian, China, as well as their gas-particle partitioning. The total concentrations of OPEs in the gas phase were in the range of 0.056-6.38 ng/m 3 with the mean concentration of 0.83 ± 1.24 ng/m 3 , while the concentrations of OPEs in the PM 2.5 were in the range of 0.32-3.46 ng/m 3 with the mean concentration of 1.21 ± 0.67 ng/m 3 . Tris-(1-chloro-2-propyl) phosphate (TCIPP) was the dominant congener in the gaseous phase, followed by tris-(2-chloroethyl) phosphate (TCEP) and tri-n-butylphosphate (TNBP), whereas TCEP was the dominant species in the PM 2.5 , followed by TCIPP and triphenyl phosphate (TPHP). Seasonality was discovered for OPEs in both gas phase and PM 2.5 with their concentrations higher in hot seasons, which may due to the temperature-driven emission or gas-particle partitioning. The PM 2.5 -bound fractions of OPEs varied significantly between seasons. Tricresyl phosphate (TMPP), tri(2-ethylhexyl) phosphate (TEHP), 2-ethylhexyl diphenyl phosphate (EHDPP), and TPHP were mostly adsorbed onto fine particles, while TNBP, TCEP, TCIPP, and tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) distributed in both gas and fine particle phases. The predicted PM 2.5 -bound fractions by K oa -based model were closer to the measurements for TCIPP, TDCIPP, and TPHP, whereas the predictions by Junge-Pankow model were closer to the measurements for TMPP and tris (2-butoxyethyl) phosphate (TBOEP). However, the predictions of both models cannot accurately match the measured gas-particle partitioning of TNBP and TCEP., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Nuclear factor-Y (NF-Y) consists of three evolutionary conserved subunits including NF-YA, NF-YB, and NF-YC; it is a critical transcriptional regulator of lipid and glucose metabolism and adipokine biosynthesis that are associated with type 2 diabetes mellitus (T2DM) occurrence, while the impacts of genetic variants in the NF-Y gene on the risk of T2DM remain to be investigated. In the present study, we screened five single-nucleotide polymorphisms (SNPs) with the SNaPshot method in 427 patients with T2DM and 408 healthy individuals. Subsequently, we analyzed the relationships between genotypes and haplotypes constructed from these SNPs with T2DM under diverse genetic models. Furthermore, we investigated the allele effects on the quantitative metabolic traits. Of the five tagSNPs, we found that three SNPs (rs2268188, rs6918969, and rs28869187) exhibited nominal significant differences in allelic or genotypic frequency between patients with T2DM and healthy individuals. The minor alleles G, C, and C at rs2268188, rs6918969, and rs28869187, respectively, conferred a higher T2DM risk under a dominant genetic model, and the carriers of these risk alleles (either homozygotes of the minor allele or heterozygotes) had statistically higher levels of fasting plasma glucose, cholesterol, and triglycerides. Haplotype analysis showed that SNPs rs2268188, rs6918969, rs28869187, and rs35105472 formed a haplotype block, and haplotype TTAC was protective against T2DM (OR = 0.76, 95% CI = 0.33-0.82, P = 0.004), while haplotype GCCG was associated with an elevated susceptibility to T2DM (OR = 2.33, 95% CI = 1.43-3.57, P = 0.001). This study is the first ever observation to our knowledge that indicates the genetic variants of NF-YA might influence a Chinese Han individual's occurrence of T2DM.

Concentrations and temporal variations of priority polycyclic aromatic hydrocarbons (PAHs) in the air from a suburban area of Dalian, China were investigated for a 1-year period to assess their sources and potential correlations with six criteria air pollutants and meteorological parameters. The total concentrations of PAHs were in the range of 4.32-112.2 ng/m 3 (Mean = 52.37 ± 23.99 ng/m 3 ). Seasonality was discovered with the PAHs following an order of winter > spring > summer > autumn. The impacts of meteorological parameters on PAH levels were season-dependent. High temperature may increase the air concentrations of 4-ring PAHs during the non-heating period, whereas high relative humidity may raise the concentrations of 3- and 4-ring PAHs during the heating period. Correlations of PAHs with criteria air pollutants, such as SO 2 , NO 2 , and O 3 , indicated that both fossil fuel combustion and photochemical oxidation influenced the air concentrations of PAHs. According to the source apportionment by diagnostic ratios and PMF model, coal combustion and traffic emission were estimated to be the main sources of PAHs in Dalian, followed by petroleum release and biomass burning. It was worth noting that the contribution of coal combustion to the PAH burdens increased from 26% to 45% due to the emission from domestic heating in winter. This extra emission needs a continuous concern in the future., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

The concentrations and seasonal variations of polycyclic aromatic hydrocarbons (PAHs) in air and seawater dissolved samples from the coastal area of Dalian were investigated, as well as their air-water exchanges. The average concentrations of PAHs were 27.5 ± 14.6 ng/m 3 and 49.5 ± 20.5 ng/L in the air and water, respectively. Phenanthrene was the dominant congener in both air and water dissolved phase. Seasonality was discovered in the air with the concentrations higher in winter than in summer, but not in the water dissolved phase. Air-water exchange trends also displayed apparent seasonality with 3-4 ring PAHs generally being volatilization or equilibrium in summer but deposition in winter, which highlighted the important influence of temperature on the air-water exchange direction of PAHs. The air-water exchange fluxes of individual PAH congeners ranged from -24331 to 6541 ng/m 2 /d, and the highest deposition and volatilization fluxes both appeared at the industrial areas, which emphasized the influence of point source emission to the magnitude of air-water diffusion flux of PAHs. Multivariate source apportionment approaches, including principle component analysis, diagnostic ratios, and positive matrix factorization, were conducted, which suggested that PAHs in water originated from multiple sources. Frequent port transport correlated vehicle/ship emission rather than coal combustion may be the primary contributor of PAHs to the coastal air and water., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Objectives: To assess the anthropometric characteristics of normoglycaemic individuals who subsequently developed hyperglycaemia, and to evaluate the validity of these measures to predict prediabetes and diabetes., Design: A community-based prospective cohort study., Participants: In total, 1885 residents with euglycaemia at baseline from six communities were enrolled., Setting: Sichuan, southwest China., Primary Outcome Measures: The incidences of prediabetes and diabetes were the primary outcomes., Methods: The waist-to-height ratio (WHtR), body mass index (BMI), waist circumference (WC) and waist-to-hip ratio (WHR) of all participants were measured at baseline and during follow-up. A 75 g glucose oral glucose tolerance test was conducted at each survey., Results: During a median of 3.00 (IQR: 2.92-4.17) years follow-up, the cumulative incidence of isolated impaired fasting glucose (IFG), isolated impaired glucose tolerance (IGT), IFG combined with IGT (IFG+IGT), and newly diagnosed diabetes mellitus (NDDM) were 8.44%, 18.14%, 8.06% and 13.79%, respectively. WHtR, BMI, WC and WHR were significantly different among subjects who subsequently progressed to isolated IFG or IGT, IFG+IGT or NDDM (p<0.05). The anthropometric characteristics of IFG+IGT subjects were similar to those of the NDDM population (p>0.005). All the baseline anthropometric measurements were useful for the prediction of future prediabetes and NDDM (p<0.05). The optimal thresholds for the four measurements were calculated for the prediction of hyperglycaemia, with a WHtR value of 0.52 performing best to identify isolated IFG or IGT, IFG+IGT and NDDM., Conclusions: Anthropometric measures, especially WHtR, could be used to predict hyperglycaemia 3 years in advance. Distinct from isolated IFG and IGT, the individuals who developed combined IFG+IGT had identical anthropometric profiles to those who progressed to NDDM., Competing Interests: Competing interests: None declared., (© Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.)

Evidence has shown that endoplasmic reticulum (ER) stress was involved in the progression to type 2 diabetes mellitus (T2DM) and development of insulin resistance. Mesencephalic astrocyte-derived neurotrophic factor (MANF) is a novel secreted protein upregulated by ER stress. This study aimed to assess serum level of MANF in normal glucose tolerance (NGT) participants and newly diagnosed prediabetic and T2DM patients. A total of 257 participants with NGT, newly diagnosed prediabetes or T2DM were recruited from Yinchao and Hangtian communities of Chengdu, Sichuan, China. Serum MANF level was quantified by enzyme-linked immunosorbent assay (ELISA). The mean age for the 257 participants (147 females) was 62±8 years (range 44-78): 71 with NGT, 115 with newly diagnosed prediabetes and 71 with T2DM. Mean serum MANF level was significantly higher with newly diagnosed prediabetes and T2DM than NGT (2.89±1.09 and 3.03±1.73 vs 2.13±1.37 ng/mL, both p<0.001). MANF level was not correlated with insulin sensitivity indexes (homeostasis model assessment for insulin resistance [HOMA-IR], Matsuda Index and quantitative insulin sensitivity check index [QUICKI]) for NGT and T2DM participants but was correlated with such indexes for prediabetes patients. We concluded that serum MANF level was higher in patients with newly diagnosed prediabetes and T2DM than in NGT controls. MANF appears to be associated with Matsuda Index, QUICKI and HOMA-IR in prediabetes patients.

The study was conducted to investigate the potential of liquorice extract (LE) from Glycyrrhiza uralensis as a dietary supplement for sheep to improve antioxidant capacity of meat. Fifty Tan sheep were randomly allocated to five groups with LE supplementation at levels of 0, 1000, 2000, 3000 and 4000 mg/kg feed. After 120 days, the longissimus thoracis muscle was sampled and conditioned for 0, 2, 4, 6 and 8 days at 4 °C. The results revealed that LE scavenged free radical in a dose-response manner in vitro. Supplementation with LE in animal diet increased (P<0.05) antioxidant content and radical scavenging activity while it decreased (P<0.05) reactive oxygen species (ROS) and thiobarbituric acid reactive substance (TBARS) levels of meat. Dietary LE supplementation can improve antioxidant capacity of meat, and the optimum dosage range of LE supplementation appeared to be 3000 to 4000 mg/kg feed., (Copyright © 2015 Elsevier Ltd. All rights reserved.)

Aims: To investigate alterations of insulin resistance (IR), chronic inflammation and urine albumin-to-creatinine ratio (ACR) in Chinese community-based prediabetic population., Materials and Methods: 252 prediabetics [prediabetes (PD), including impaired fasting glucose (IFG), 91; impaired glucose tolerance (IGT), 123; IFG+IGT, 38] and 38 newly diagnosed-diabetics (NDDM) aged over 35 years older were screened from 2336 community individuals. 123 age and gender matched individuals with normal glucose tolerance (NGT) were selected as controls. Serum adiponectin, interleukin-6 (IL-6) levels and urine ACR were determined, HOMA-IR and Gutt's index were calculated to evaluate IR and insulin sensitivity, respectively., Results: The data displayed significant difference of serum adiponectin, IL-6, ACR and Gutt's index among PD, NDDM and NGT groups. Adiponectin level and Gutt's index decreased, but IL-6 level and ACR increased gradually among NGT, PD and NDDM groups (P<0.01). Unlike adiponectin and IL-6, ACR analysis indicates a gradual increase from NGT, IFG, IGT, IFG+IGT to NDDM individuals (P<0.01). Gutt's index showed significant difference between IFG and NDDM, IFG+IGT and NDDM (P<0.01), but HOMA-IR index did not., Conclusions: IR, chronic inflammation and endothelial dysfunction dose exist in prediabetic individuals, especially in IFG+IGT population. Gutt's index and ACR might seem to be more sensitive than adiponectin and HOMA-IR index as IR and chronic inflammation maker in prediabetic population.