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Lack of knowledge on the destiny of organic micropollutants (OMPs) in the Tibetan Plateau region of China prevents the public from being aware of the need for protecting these unique aquatic ecosystems that are precious water resources and source areas of the Yellow River. To address this knowledge gap, this study systematically investigated the multi-residue analysis, distribution, and potential risks of six types of OMPs, namely, neonicotinoid pesticides (NEOs), fungicides, organophosphate esters (OPEs), organophosphorus pesticides (OPPs), psychoactive substances (PSs), and antidepressants (ADs), in surface waters of major cities in Qinghai. A total of 31 compounds, consisting of 8 NEOs, 1 fungicide, 12 OPEs, 2 OPPs, 5 PSs, and 3 ADs, were detected in >50% of the sites, showing their ubiquitous nature in the study area. Results showed that the total OMP concentration in surface water was 28.3–908 ng/L, and OPEs were the dominant composition (48.6%–97.4%). The risk quotient values of the detected diazinon and dursban regularly exceeded 1 for aquatic organisms at all sampling sites, indicating moderate–high chronic ecological risk. The joint probability curves showed that dursban and NEOs have higher risk levels than other OMPs. Although the results of the non-carcinogenic total hazard quotient of the OMPs in the surface water was less than 1 in all age groups and the carcinogenic risk was lower than the negligible risk level, the potential risks to children and infants were considerably greater and should not be underestimated. In addition to pollutant concentration and exposure duration, ingestion rate and body weight (BW) are also important factors affecting health risk, with BW having a negative effect. To the best of the authors' knowledge, this report is the first to describe OMP pollution in Qinghai, and the results provide new insight into the ecological security of the water resources of the Tibetan Plateau. [Display omitted] • The occurrence of 67 organic micropollutants of surface water in Qinghai was first determined. • Organophosphate esters were dominant organic micropollutants, followed by neonicotinoid pesticides. • The environmental risks of organic micropollutants were assessed based on the risk quotient values and joint probability curves. • Diazinon and dursban had moderate to high chronic ecological risk at all sites. • The priority substances lists provide significant references on pollutant control. [ABSTRACT FROM AUTHOR]

Two large successive co-site landslide dams blocked the Jinsha River in the Sichuan Province of China in 2018. A quantitative risk analysis was carried out to quantify potential human and economic losses resulting from the failure of these dams, especially the interaction between the co-site landslide dams, and to further investigate the influence of digging a diversion channel on risk mitigation. Flood routing for three scenarios (i.e. after the first dam formation, when the second landslide mass was added, and with a diversion channel after failures) were simulated using HEC-RAS. The human and economic losses were evaluated using a human risk assessment model together with empirical formulations. The results show that the risk of breaching floods had increased significantly after the second co-site landslide dam formation on the pre-existing loose deposits of the first dam. This amplification effect of outburst floods was so great that the peak outflow resulting from the breaching of the second landslide dams was more important, leading to greater economic losses than those resulting from the breaching of the first dam. However, the expected loss of life caused by the breach of the two landslide dams appeared small because of the sufficient time lag provided by the long distance between the residential area and the dam site. The simulations also outline the importance of the diversion channel in decreasing the peak outflow rate and hence downstream risks. A parametric analysis shows that a deep channel with a moderate longitudinal gradient can significantly decrease the peak outflow discharge at the dam site. Flood intensity and risks to downstream towns did not change because of the relatively small attenuation rate of the peak outflow rate. However, a shorter height of the residual dam can be obtained by optimizing the diversion channel, and the amplification effects of such co-site dam can be significantly reduced in the future. • The human and economic risks caused by the two co-site Baige landslide dams are quantitatively assessed. • The reasons why an amplification effect of flood intensity and downstream risks occurred are systematically investigated. • An optimal diversion channel is obtained based on parametric analysis of the peak outflow rate during dam breach. • The influence of the diversion channel in flood intensity, risks in downstream and risk mitigation is discussed. [ABSTRACT FROM AUTHOR]

Background: The safety assessment and control of stacked transgenic crops is increasingly important due to continuous crop development and is urgently needed in China. The genetic stability of foreign genes and unintended effects are the primary problems encountered in safety assessment. Omics techniques are useful for addressing these problems. The stacked transgenic maize variety 12–5 × IE034, which has insect-resistant and glyphosate-tolerant traits, was developed via a breeding stack using 12–5 and IE034 as parents. Using 12–5 × IE034, its parents (12–5 and IE034), and different maize varieties as materials, we performed proteomic profiling, molecular characterization and a genetic stability analysis. Results: Our results showed that the copy number of foreign genes in 12–5 × IE034 is identical to that of its parents 12–5 and IE034. Foreign genes can be stably inherited over different generations. Proteomic profiling analysis found no newly expressed proteins in 12–5 × IE034, and the differences in protein expression between 12 and 5 × IE034 and its parents were within the range of variation of conventional maize varieties. The expression levels of key enzymes participating in the shikimic acid pathway which is related to glyphosate tolerance of 12–5 × IE034 were not significantly different from those of its parents or five conventional maize varieties, which indicated that without selective pressure by glyphosate, the introduced EPSPS synthase is not has a pronounced impact on the synthesis of aromatic amino acids in maize. Conclusions: Stacked-trait development via conventional breeding did not have an impact on the genetic stability of T-DNA, and the impact of stacked breeding on the maize proteome was less significant than that of genotypic differences. The results of this study provide a theoretical basis for the development of a safety assessment approach for stacked-trait transgenic crops in China. [ABSTRACT FROM AUTHOR]

Background: This study aims to evaluate the ability of laboratories to perform spinal muscular atrophy (SMA) genetic testing in newborns based on dried blood spot (DBS) samples, and to provide reference data and advance preparation for establishing the pilot external quality assessment (EQA) scheme for SMA genetic testing of newborns in China., Methods: The pilot EQA scheme contents and evaluation principles of this project were designed by National Center for Clinical Laboratories (NCCL), National Health Commission. Two surveys were carried out in 2022, and 5 batches of blood spots were submitted to the participating laboratory each time. All participating laboratories conducted testing upon receiving samples, and test results were submitted to NCCL within the specified date., Results: The return rates were 75.0% (21/28) and 95.2% (20/21) in the first and second surveys, respectively. The total return rate of the two examinations was 83.7% (41/49). Nineteen laboratories (19/21, 90.5%) had a full score passing on the first survey, while in the second survey twenty laboratories (20/20, 100%) scored full., Conclusions: This pilot EQA survey provides a preliminary understanding of the capability of SMA genetic testing for newborns across laboratories in China. A few laboratories had technical or operational problems in testing. It is, therefore, of importance to strengthen laboratory management and to improve testing capacity for the establishment of a national EQA scheme for newborn SMA genetic testing.

Background: We aimed to evaluate the diagnostic capabilities of Chinese laboratories for inherited metabolic disorders (IMDs) using gas chromatography-mass spectrometry (GC-MS) on urine samples. Meanwhile, based on the result of the pilot external quality assessment (EQA) scheme, we hope to establish a standardized and reliable procedure for future EQA practice., Methods: We recruited laboratories that participated in the EQA of quantitative analysis of urinary organic acids with GC-MS before joining the surveys. In each survey, a set of five real urine samples was distributed to each participant. The participants should analyze the sample by GC-MS and report the "analytical result", "the most likely diagnosis", and "recommendation for further tests" to the NCCL before the deadline., Results: A total of 21 laboratories participated in the scheme. The pass rates were 94.4% in 2020 and 89.5% in 2021. For all eight IMDs tested, the analytical proficiency rates ranged from 84.7% - 100%, and the interpretational performance rate ranged from 88.2% - 97.0%. The performance on hyperphenylalaninemia (HPA), 3-methylcrotonyl-CoA carboxylase deficiency (MCCD), and ethylmalonic encephalopathy (EE) samples were not satisfactory., Conclusions: In general, the participants of this pilot EQA scheme are equipped with the basic capability for qualitative organic acid analysis and interpretation of the results. Limited by the small size of laboratories and samples involved, this activity could not fully reflect the state of clinical practice of Chinese laboratories. NCCL will improve the EQA scheme and implement more EQA activities in the future.

Wastewater-based epidemiology (WBE) is considered a cost-effective alternative approach capable of determining the consumption and prevalence of drug use in communities, however, the application of WBE for estimating the prevalence of depression has seldom been reported. In this study, the prevalence of antidepressants was estimated in five cities in Qinghai Province, west China to examine the feasibility of using WBE to estimate the depression prevalence. Residual concentrations of the drugs varied from different wastewater treatment plants (WWTPs) in five cities. Venlafaxine (0.06-720 ng/L), O-desmethylvenlafaxine (1.31-1659 ng/L), paroxetine (