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• Detailed land use transitions from 2000–2020 were analyzed. • LUF-PLE framework quantified Xianghai wetland's PLES functional dynamics. • CCDM elucidate the harmonized evolution of the PLES functions. With the global alterations in ecological conditions and population growth, the study of production-living-ecological space (PLES) offers a new perspective in balancing land resource utilization with the sustainable development of society, economy, and environment. China has incorporated the optimization of PLES into its sustainable development strategy. However, research concerning the PLES functions within wetland ecosystems remains notably sparse. Wetlands, distinguished by their unique ecosystem, command a special place in the realm of production, living and ecology research. Their importance is multifaceted, stemming from their roles in ecology, economy, and socio-cultural dimensions, and further accentuated by their distinct geographical, climatic, and biodiversity attributes. In light of this, the Xianghai Wetland Nature Reserve in Jilin, China, was chosen as the focal point of this study to probe into the coupling coordination degree of its PLES functions. The study commenced with a thorough analysis of the changes in land use types from 2000 to 2020 using a land use transition matrix model. Following this, a customized land use function assessment framework called LUF-PLE was formulated, offering a quantitative assessment of the Xianghai wetland's production, living, and ecological space functions and shedding light on their spatiotemporal dynamics. Finally, a coupling coordination degree model (CCDM) was employed to elucidate the evolution of the PLES functions of Xianghai wetlands. Key findings revealed that: (1) the Xianghai wetland's PLES coupling coordination degree exhibited a pattern of decline, stabilizing post-2010, with the most pronounced dip observed between 2000–2010; (2) the coordination between production and living functions was notably subdued, whereas the coordination between production and ecological functions saw the steepest decline. In the future, a sustainable wetland development strategy should be formulated and implemented based on the observed trends in coupling coordination and the spatiotemporal differentiation of production-liveling-ecological functions in the local context. This research furnishes scientific substantiation and serves as a reference for the sustainable stewardship and preservation of wetlands. [ABSTRACT FROM AUTHOR]

A mutation in the epithelial morphogen gene ectodysplasin-A1 (EDA1) is responsible for the disorder X-linked hypohidrotic ectodermal dysplasia (XLHED), the most common form of ectodermal dysplasia. XLHED is characterized by impaired development of hair, eccrine sweat glands, and teeth. This study aimed to identify potentially pathogenic mutations in four Chinese XLHED families.Genomic DNA was extracted from the peripheral blood and sequenced. Sanger sequencing was used to carry out mutational analysis of the EDA1 gene, and the three-dimensional structure of the novel mutant residues in the EDA trimer was determined. Transcriptional activity of NF-κB was tested by Dual luciferin assay.We identified a novel EDA1 mutation (c.1046C>T) and detected 3 other previously-reported mutations (c.146T>A; c.457C>T; c.467G>A). Our findings demonstrated that novel mutation c.1046C>T (p.A349 V) resulted in XLHED. The novel mutation could cause volume repulsion in the protein due to enlargement of the amino acid side chain. Dual luciferase assay revealed that transcriptional NF-κB activation induced by XLHED EDA1 protein was significantly reduced compared with wild-type EDA1.These results extend the spectrum of EDA1 mutations in XLHED patients and suggest a functional role of the novel mutation in XLHED.