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Specimens of Clavulinopsis (Clavariaceae, Agaricales) collected in China were studied using morphological and molecular methods. Six species—C. aspersa, C. bicolor, C. bispora, C. erubescens, C. incarnata, and C. tropicalis—are described as new to science, and C. trigonospora is a newly recorded species in China. Phylogenetic analysis was conducted based on a combined dataset of internal transcribed spacer and nuclear ribosomal RNA large subunit sequences. The phylogenetic reconstruction revealed that the six new species each formed an independent lineage, and the samples of C. trigonospora from China were nested with accessions of C. trigonospora collected from Italy. The morphology of the seven Chinese species is described in detail, and is illustrated with line drawings and photographs. A key to the known Clavulinopsis species in China is provided. [ABSTRACT FROM AUTHOR]

Previous studies on the relationship between environmental regulation (ER) and technological innovation efficiency of defence industry (TIE-DI) mainly focus on variability and complexity, while few empirical studies have incorporated environmental flexibility into models, and most of them are based on questionnaires. Therefore, this paper takes environmental regulation and environmental policy complexity (EPC) as the entry point, so as to discuss the feasibility of improving the technological innovation efficiency of China's defence industry enterprises (CDI), which aims to empirically test the mechanism of ER and EPC on improving the efficiency of applied technological innovation of China's defence industry enterprises. The research conclusion provides sufficient theoretical basis and empirical support for strengthening the technical innovation efficiency support, standardising the market order and the market leading, establishing the information disclosure mechanism and improving the internal control of industrial enterprises. [ABSTRACT FROM AUTHOR]

The development of sequence decomposition techniques in recent years has facilitated the wide use of decomposition-based prediction models in hydrological forecasting. However, decomposition-based prediction models usually use the overall decomposition (OD) sampling technique to extract samples. Some studies have shown that the OD sampling technique causes abnormally "high" performance of models owing to the utilization of future information, and this technique cannot be applied in practice. Several researchers have also proposed novel sampling techniques, such as semi-stepwise decomposition (SSD), fully stepwise decomposition (FSD), and single-model SSD (SMSSD). Moreover, an improved single-model FSD (SMFSD) sampling technique is proposed in this study. Four decomposition methods, namely discrete wavelet transform (DWT), empirical mode decomposition (EMD), complete ensemble empirical mode decomposition with adaptive noise (CEEMDAN), and variational mode decomposition (VMD), are introduced in this study. A systematic investigation of the models developed using OD sampling techniques is conducted, and the applicability of SSD, FSD, SMSSD, and SMFSD sampling techniques is reasonably evaluated. The application of monthly runoff prediction using the five sampling techniques and four decomposition methods at five representative hydrological stations in Poyang Lake, China, shows that (1) EMD and CEEMDAN (including the improved EMD-based adaptive decomposition method) cannot be used to construct stepwise decomposition prediction models because the implementation of the stepwise decomposition strategy leads to a variable number of sub-series. (2) OD sampling techniques cannot develop convincing models for practical prediction because future information is introduced into the samples for model training. (3) Models developed based on SSD and SMSSD sampling techniques do not use future information in the training process, but suffer from severe overfitting and inferior prediction performance. (4) Models developed based on FSD and SMFSD sampling techniques can produce convincing prediction results, and the combination of the proposed SMFSD sampling technique and VMD develops prediction models with superior performance and significantly enhances the efficiency of the models. [ABSTRACT FROM AUTHOR]

Reference Crop evapotranspiration (ET0) datasets based on reanalysis products can make up for the time discontinuity and the spatial insufficiency of surface meteorological platform data, which is of great significance for water resources planning and irrigation system formulation. However, a rigorous evaluation must be conducted to verify if reanalysis products have application values. This study first evaluated the ability of the second-generation China Meteorological Administration Land Data Assimilation System (CLDAS) dataset for officially estimating ET0 (the local meteorological station data is used as the reference dataset). The results suggest that the temperature data of CLDAS have high accuracy in all regions except the Qinghai Tibet Plateau (QTP) region. In contrast, the global solar radiation data accuracy is fair, and the relative humidity and wind speed data quality are poor. The overall accuracy of ET0 is acceptable other than QTP, but there are also less than 15% (103) of stations with significant errors. In terms of seasons, the error is largest in summer and smallest in winter. Additionally, there are inter-annual differences in the ET0 of this data set. Overall, the CLDAS dataset is expected to have good applicability in the Inner Mongolia Grassland area for estimating ET0, Northeast Taiwan, the Semi Northern Temperate zone, the Humid and Semi Humid warm Temperate zone, and the subtropical region. However, there are certain risks in other regions. In addition, of all seasons, summer and spring have the slightest bias, followed by autumn and winter. From 2017 to 2020, bias in 2019 and 2020 are the smallest, and the areas with large deviation are south of climate zone 3, the coastal area of climate zone 6, and the boundary area of climate zone 7. [ABSTRACT FROM AUTHOR]

The life cycle of the cabbage beetle Colaphellus bowringi in southeastern China is complex due to four options for adult development: summer diapause, winter diapause, prolonged diapsuse, and nondiapause. However, detailed information on the multi‐year emergence patterns of diapausing individuals in this beetle has not been documented. In this study, we monitored the adult emergence patterns of diapausing individuals and estimated the influence of the diapause‐inducing temperature and photoperiod on the incidence of prolonged diapause under seminatural conditions for several years. The duration of diapause for adults collected from the vegetable fields in different years varied from several months to 5 years. Approximately 25.9%–29.2% of individuals showed prolonged diapause (emergence more than 1 year after entering diapause) over the 5 years of observation. Furthermore, regardless of insect age, the emergence of diapausing adults from the soil always occurred between mid‐February and March in spring and between late August and mid‐October in autumn, when the host plants were available. The influence of diapause‐inducing temperatures (22, 25, and 28°C) combined with different photoperiods (L:D 12:12 h and L:D 14:10 h) on diapause duration was tested under seminatural conditions. Pairwise comparisons of diapause duration performed by the log‐rank test revealed that the low temperature of 22°C combined with the long photoperiod of L:D 14:10 h induced the longest diapause duration, whereas the low temperature of 22°C combined with the short photoperiod of L:D 12:12 h induced the highest proportion of prolonged diapause. This study indicates that C. bowringi adopts a multi‐year dormancy strategy to survive local environmental conditions and unpredictable risks. [ABSTRACT FROM AUTHOR]

Background: Pregnancy termination is the only effective treatment for preeclampsia. However, there are controversies on the selection of modes of delivery. The objective was to evaluate whether mode of delivery in labor differentially affected the rate of adverse maternal outcomes related to severe preeclampsia. Objective: This study aimed to evaluate whether the modes and timings of delivery affects adverse maternal outcomes in pre-eclampsia. Methods: Clinical data from 2516 singleton pregnant women with severe preeclampsia were collected in a multicenter, large-sample, cross-sectional study in mainland China. The patients were divided into cesarean-delivery (CD) and vaginal-delivery (VD) categories and then into Group 1 (≤ 27+ 6 weeks), Group 2 (28– 33+ 6 weeks), Group 3 (34– 36+ 6 weeks), and Group 4 (≥ 37 weeks) according to the mode of delivery and gestational weeks. All data were exported into the SPSS software and analyzed by the Student's t-tests or Mann–Whitney U-tests and the chi-squared test. Results: A total of 2516 singleton pregnant women with severe preeclampsia were collected and the overall cesarean section rate was 84.9%. The vaginal delivery rates among the four groups were significantly different with 70%, 19.7%, 6.6%, 15.1% in groups 1, 2, 3, 4, respectively (P< 0.05), while perinatal mortality was lower in the CD groups than VD groups (3.3% vs 50.4%, P< 0.05). The neonatal asphyxia rate was significantly higher with CD than with VD in Group 2 (36.4% vs 12.9%, P< 0.05). The perinatal mortality with CD, 3, and 4 was significantly lower than with VD (10.0% vs 68.5% in Groups 2, 2.3% vs 28.3% in Groups 3, 0.8% vs 5.6% in Groups 4, all P< 0.05). Conclusion: Most pregnant women with severe preeclampsia opted for a cesarean section in China. The lower perinatal mortality was associated with cesarean section, but the rate of maternal PPH or mortality was not related with the mode of delivery. So cesarean section is the safer delivery mode for the pregnant women complicated with severe preeclampsia. [ABSTRACT FROM AUTHOR]

Objective: To characterize the influence of relative wall thickness (RWT) on Cornell, Sokolow-Lyon and Peguero-Lo Presti voltages and elucidate its potential impacts on their diagnostic accuracy for LVH in a large general Chinese population.Methods: A total of 10,614 permanent residents aged ≥ 35 years were recruited for this study. All the participants were subjected to ECG and echocardiogram during the same visit. Multivariate linear and logistic regression analyzes were conducted to assess the influence of RWT on the voltages and their diagnostic performance for LVH detection.Results: A distinct correlation was identified between RWT and Cornell and Peguero-Lo Presti voltages following adjustments for age, gender and left ventricular mass (LVM) (β = 0.675 and 1.342, respectively; Ps < 0.001). Besides, subjects with RWT > 0.42 exhibited higher rates of LVH diagnosed by Cornell (OR = 1.78, 95% CI: 1.45-2.20), Sokolow-Lyon (OR = 1.30, 95% CI: 1.08-1.56), and Peguero-Lo Presti voltage (OR = 1.48, 95% CI: 1.29-1.70) after adjustments for age, gender and echocardiographic LVH. Furthermore, concentric remodeling or concentric hypertrophy displayed higher rates of LVH diagnoses via Cornell and Peguero-Lo Presti voltage criteria, as compared with normal geometry or eccentric hypertrophy, respectively (all Ps < 0.05), findings of which were independent of age, gender and LVMI.Conclusion: Echocardiographic RWT was independently correlated with electrocardiographic voltage measures of LVH, which influenced their positive rates and contributed to poor diagnostic performance. [ABSTRACT FROM AUTHOR]

Genetic diversity influences the fitness of species and provides variation for adaptation. Garcinia paucinervis Chun et How (Clusiaceae) is an endangered species with important ecological, medicinal and ornamental values endemic to Southwest China and Northern Vietnam, whose populations were severely fragmented in island habitats and population sizes were influenced by human. The assessment of genetic variation of G. paucinervis is anticipated to provide essential information for efficient conservation strategies. In this study, a suite of population genetics tests and analyses were used to investigate genetic diversity and structure of the 11 natural populations (a total of 360 individuals) of G. paucinervis in Guangxi and Yunnan Provinces, China, based on genotypes at 14 loci. Our results revealed a low to moderate genetic diversity in G. paucinervis remnants (HE = 0.487, I = 0.924, AR = 3.420). The global inbreeding coefficient (FIS = 0.004) showed significant deviation from Hardy–Weinberg equilibrium, implying that the risk of inbreeding depression accompanied by heterozygote deficiency was probably due to severe habitat fragmentation and decreasing population sizes. Significant bottlenecks were detected in two populations. There has been little recent exchange of genes between most of the population pairs. Mantel test revealed that the genetic distance was not related to the geographical distance, suggesting a limitation of gene flow. A population from Yunnan Province could be classified as an independent cluster separated from the other populations, which should be considered as a prior conservation unit. [ABSTRACT FROM AUTHOR]

Coal pillars are formed by excavation and mining activities in an intact coal seam and play a key role in underground coal mines. Most previous investigations of pillars have mainly focused on existing coal pillar failures. However, few scholars have investigated the failure mechanisms of coal pillars during their formation process. This paper focuses on the failure of a 7 m-wide coal pillar that caused the large deformation of a tailgate in the Sijiazhuang coal mine in China. Field tests and numerical modeling were used to study the initiation, propagation, and failure of cracks within this gob-side coal pillar during its formation. Field monitoring revealed that the maximum roof-to-floor and rib-to-rib convergence reached 860 mm and 1460 mm, respectively. The coal pillar became a yield pillar with substantial fractures. A numerical model was built using UDEC Trigon logic and calibrated with laboratory tests and RQD methods. Both the natural roadway deformation and crack distribution in the coal pillar were simulated. A FISH function was used to document the propagation of shear and tensile cracks in pillars with different W/H ratios, and a damage parameter was adopted to evaluate the failure of these pillars. The results suggest that the most appropriate pillar width is 10 m. Field trials prove that a 10 m-wide coal pillar combined with optimized support measures can effectively control deformation around the tailgate. [ABSTRACT FROM AUTHOR]

Guizhou Karst Plateau is located at the center of the karst region in Asia, where landslides are a typical disaster. Affected by the local karst environment, the landslides in this region have their own characteristics. In this study, 3975 landslide records from inventories of the Guizhou karst plateau are studied. The geographical detector method is used to detect the dominant casual factor and predominant multi-factor combinations for the local landslides. The results show that landslides are prone to areas on slopes between 10° and 35°, of clay rock, in close proximity to gullies, and especially in areas of moderate vegetation, dryland, and mild rocky desertification. Continuous precipitation over 10 days has a great effect on landslide occurrence. Compared with the individual factors, the impact of two-factor interaction has greater explanatory power for landslide volume. The volume of earthquake-induced landslides is predominantly controlled by the interactions of faults and slopes, while that of human-induced landslides is affected by the interactions of land cover and hydrological conditions. For rainfall-induced landslides, the dominant interactions vary in different regions. In the central karst basin, the interactions between faults and precipitation can explain over 90% of the variations in landslide volumes. In the southern hilly karst region, the interactions between lithology and slope can explain over 71% of the variations in landslide volume and those between fault and land-use can explain 50% of the variations of the landslide volumes in the northeastern mountainous karst region. [ABSTRACT FROM AUTHOR]

In recent years, multi-source clustering has received a significant amount of attention. Several multi-source clustering methods have been developed from different perspectives. In this paper, aiming at addressing the problem of Chinese–Tibetan bilingual document clustering, a novel bilingual clustering scheme is proposed, which can well capture both the intralingua document structures and interlingua document relations. The proposed scheme consists of three major phases. Firstly, to properly combine the feature structures of documents in different languages, a bilingual graph is constructed. In the second phase, two bilingual similarity matrices are computed based on the random walk performed in the bilingual graph. Finally, the similarity based clustering methods are performed on the two bilingual similarity matrices so as to generate cluster structures for documents in each language respectively, which lead to the corresponding bilingual clustering methods. Extensive experiments conducted on two Chinese–Tibetan bilingual document sets have confirmed the effectiveness of the proposed methods. [ABSTRACT FROM AUTHOR]

Hyper-spectral data is widely used to determine soil properties. However, few studies have explored the soil spectral characteristics as response to soil erosion. This study analysed the spectral response of different eroded soils in subtropical China, and then identify the spectral characteristics and soil properties that better discriminate soils with different erosion degrees. Two methods were compared: direct identification by inherent spectral characteristics and indirect identification by predictions of critical soil properties. Results showed that the spectral curves for different degrees of erosion were similar in morphology, while overall reflectance and characteristics of specific absorption peaks were different. When the first method is applied, some differences among different eroded groups were found by integration of associated indicators. However, the index of such indicators showed apparent mixing and crossover among different groups, which reduced the accuracy of identification. For the second method, the correlation between critical soil properties, such as soil organic matter (SOM), iron and aluminium oxides and reflectance spectra, was analysed. The correlation coefficients for the moderate eroded group were primarily between −0.3 to −0.5, which were worse than the other two groups. However, the maximum value of R was obtained as 0.86 and 0.94 for the non-apparent eroded and the severe group. Furthermore, these two groups also showed some differences in the spectral response of iron complex state (Fep), Aluminium amorphous state (Alo) and the modelling results for soil organic matter (SOM). The study proved that it is feasible to identify different degrees of soil erosion by hyper-spectral data, and that indirect identification by modelling critical soil properties and reflectance spectra is much better than direct identification. These results indicate that hyper-spectral data may represent a promising tool in monitoring and modelling soil erosion. [ABSTRACT FROM AUTHOR]

Hyperspectral reflectance is widely used for determining important properties of soil erosion. However, there have been few studies which focus on the influence of soil erosion intensity on the characteristics of hyperspectral reflectance, and such information would provide a new tool to improve quantitative understanding of soil erosion. In this study, 35 soil samples were collected from three regions with different erosion intensities in Changting County, a typical severely eroded county in the ferralic cambisol region of southern China, and classified into three groups according to different erosion controlling status. All the samples were scanned at wavelengths from 400 to 2,498 nm by an ASD Field Spec Portable Spectrometer, and the erosion intensity of each sample was calculated using the Revised Universal Soil Loss Equation. Multivariate stepwise linear regression was then employed to model the soil erosion intensity based on reflectance. The results suggested that the absorption peaks of each sample were in a similar wavelength range, while the absorption depth varied with different erosion status, and the reflectance of extremely eroded soil samples were the highest. During modelling of erosion intensity, the result was poor when all the samples were combined, but improved greatly at certain wavelength ranges when samples were classified into three groups based on different erosion controlling status. The extreme erosion group markedly outperformed the other two groups, in which the R values between the actual and predicted erosion intensity were 0.67, 0.85 and 0.80 for each spectral type. The results indicated that hyperspectral reflectance is a promising method for accurately monitoring erosion intensity. [ABSTRACT FROM AUTHOR]

Abstract: The importance of a better understanding of the growth response of forest to climate change for managing and conserving forest has been realized. In this study, we developed the ring-width chronologies of Korean pine (Pinus koraiensis), one of the main constructive species of Changbai Mountain in northeastern China, to examine the radial growth–climate relationships. The stability of these relationships before and after abrupt climate change was evaluated. We built regression equations to project the future growth of the species under future climate change scenarios projected by the Providing Regional Climates for Impacts Studies (PRECISs) climate model. The results were as follows: (1) The chronologies in the three elevation gradients, HY1 at 740m.s.l., FA at 940m.s.l. and HY2 at 1258m.s.l., had the good spatial similarity with high Gleichläfigkeit (GLK) indices; however, significant differences still existed between the growth–climate relationships of the three sites. The width chronology of Korean pine at site HY1 was positively correlated with the precipitation in September of the previous year (p <0.01) and June of the current year (p <0.05). The chronology at site FA was positively correlated with the temperature in March and April of the current year (p <0.05). Whereas the current July temperature and the previous September precipitation were the main limiting factors for the growth of Korean pine at site HY2. (2) Mann–Kendall test results revealed that the climatic data from the meteorological stations near the sampling sites had an abrupt annual average temperature change in 1989, but the radial growth–climate relationship change only occurred in the chronology with May precipitation at site HY2, which may be caused by water stress. (3) With the projected increasing temperature and decreasing precipitation, compared with the base-line period (1971–2000), the radial growth of Korean pine at HY1 will relatively decrease, and the reduction will gradually increase. In contrast, at the higher elevation, like the FA and HY2 sites, the radial growth of Korean pine will relatively increase. Thus, the higher elevation areas of the Korean pine’s vertical distribution belt are more favorable for this species’ radial growth and forestation. [Copyright &y& Elsevier]

Community structure and leaf traits are important elements of terrestrial ecosystems. Changes of community structure and leaf traits are of particular use in the study of the influence of climate change on terrestrial ecosystems. Patterns of community structure (including species richness, above- and below-ground biomass) and leaf traits (including leaf mass per area (LMA), nitrogen content both on mass and area bases ( N and N), and foliar δC) from 19 grassland plots along an altitudinal transect at Hongchiba in Chongqing, China, were analyzed. Species richness along the altitudinal transect had a hump-shaped pattern. Above-ground biomass had a quadratic decrease along the altitudinal gradient whereas below-ground biomass had the opposite pattern. Change of above-ground biomass of various taxonomic groups with altitude was also studied. Poaceae showed strong negative relationships and Asteraceae showed a hump-shaped relationship with increase of altitude. Five common species of the grassland, Trifolium pratense, Geranium wilfordii, Aster tataricus, Leontopodium leontopodioides, and Spiraea prunifolia, were particularly studied for variation of leaf traits along the altitudinal gradient. Averaged for all species, LMA, N and foliar δC had positive correlations with altitude. N did not change significantly as altitude increased. LMA and N showed significant positive relationships with foliar δC. The adaptive features of leaf traits among different species were not consistent. The study highlights specific adaptation patterns in relation to altitude for different plant species, provides further insights into adaptive trends of community structure and leaf traits in a specific ecological region filling a gap in the definition of global patterns, and adds to the understanding of how adaptive patterns of plants may respond to global climate change. [ABSTRACT FROM AUTHOR]

The Integrated Biosphere Simulator (IBIS)-a Dynamic Global Vegetation Model-was validated by simulating the potential natural vegetation map of China using data on monthly mean climate from 1961 to 1990, soil texture, and topography. Although the vegetation map simulated by IBIS was able to describe the sketch of vegetation patterns in China, the distributions of several plant functional types (PFTs) and vegetation types were still simulated incorrectly, especially in eastern temperate areas, southern subtropics, the southern Sichuan basin, and the Hengduan mountains area. By adjusting some of the climatic constraints and physiological parameters of PFTs defined in IBIS, the simulated distributions of PFTs became reasonable, and the simulated vegetation map fitted the natural vegetation map better. The kappa statistic between the simulated and the natural vegetation maps was 0.76, an increase of 16.9% from the previous parameter adjustment of 0.65. Correspondingly, the degree of agreement between these two maps rose from 'good' to 'very good'. After the parameter adjustments, IBIS became more suitable for the large-scale simulation of Chinese natural vegetation distributions and could provide a powerful support to reveal the dynamic responses of terrestrial ecosystems to climate change in China. [ABSTRACT FROM AUTHOR]

This study aims to investigate the expression and significance of EphA2 and EphrinA-1 in human gastric adenocarcinoma progression and prognosis. The expression of EphA2 and EphrinA-1 was detected in the cell lines and tissues of gastric adenocarcinoma. Different expression levels of EphA2 and EphrinA-1 were found in two cell lines. The expression of EphA2 and EphrinA-1 was significantly higher in gastric adenocarcinoma tissues than in normal tissues. Statistical analysis showed a significant correlation of EphA2 expression with the depth of tumor invasion, tumor-node-metastasis (TNM) stages, and lymph node metastasis. EphrinA-1 over-expression was significantly correlated with TNM stages and lymph node metastasis, while EphA2 expression was found to be an independent prognostic factor of postoperative gastric adenocarcinoma. In conclusion, the increased expression of EphA2 and EphrinA-1 plays an important role in the progression of human gastric adenocarcinoma, in which elevated EphA2 expression is an independent factor that indicates poor prognosis in postoperative gastric adenocarcinoma. [ABSTRACT FROM AUTHOR]

Multi-kilometre-thick Jurassic–Cretaceous-age sedimentary successions exposed in the southern, northwestern, and northeastern regions of the Junggar Basin display a consistent and correlative stratigraphy comprising four, stacked second-order megasequences: Badaowan, Sangonghe, Shishugou, and Kalaza. Each consists of a basal erosional unconformity or discontinuity surface and lower, middle, and upper units that are interpreted as forestepping, backstepping, and aggradational systems tracts, respectively. Each megasequence is interpreted as recording an upsection shift from active tectonism and uplift to tectonic quiescence with associated changes in crustal response and sediment supply. Basin-wide analysis of megasequences indicates that tectonism was intermittent and regionally variable. A maximum phase of subsidence and sediment accommodation is recorded in the middle unit of each megasequence and correlates with a notable abundance of fossil vertebrates suggesting a primary tectonic and basin-response control on fossil preservation. Seasonally dry climatic conditions were developed first in the northeastern region of the basin during the Pliensbachian, followed by basin-wide seasonal dryness during the Bajocian. Seasonally dry climatic conditions were permanently established across the basin by the Oxfordian and intensified during the Early Cretaceous. A seasonally dry climate from Oxfordian through the Early Cretaceous correlates positively with the widespread presence of fossil vertebrates and suggests an additional climatic control on fossil preservation.Des séquences sédimentaires (datant du Jurassique au Crétacé) de plusieurs kilomètres d'épaisseur affleurent dans les régions sud, nord-ouest et nord-est du bassin Junggar; elles présentent une stratigraphie régulière et corrélative qui comprend un empilement de quatre mégaséquences de 2[sup e] ordre : Badaowan, Sangonghe, Shishugou et Kalaza. Chacune comporte une discordance érosionnelle à la base ou une surface de discordance et des unités inférieures, médianes et supérieures que l'on interprète respectivement comme des prismes de progradation, de rétrogradation et d'aggradation. On interprète chaque mégaséquence comme la consignation d'un passage de tectonique active et de soulèvement à une quiescence tectonique avec les changements associés dans la réponse crustale et l'approvisionnement en sédiments. Une analyse des mégaséquences à la grandeur du bassin indique que la tectonique était intermittente et variait selon les régions. Une phase maximum de subsidence et d'accumulation de sédiments est consignée dans l'unité médiane de chaque mégaséquence et peut être mise en corrélation avec une abondance remarquable de vertébrés fossiles, suggérant ainsi une tectonique primaire et que la réponse du bassin contrôlait la préservation des fossiles. Des conditions climatiques saisonnières sèches se sont tout d'abord développées dans la région nord-est du bassin durant le Pliensbachien, suivies d'une sécheresse saisonnière dans tout le bassin au Bajocien. À l'Oxfordien, des conditions climatiques saisonnières sèches s'étaient établies en permanence à travers tout le bassin; ces conditions se sont intensifiées au Crétacé précoce. Un climat saisonnier sec de l'Oxfordien au Crétacé précoce peut être corrélé de façon positive avec la présence d'une grande étendue de vertébrés fossiles et suggère un contrôle climatique additionnel sur la préservation des fossiles.[Traduit par la Rédaction] [ABSTRACT FROM AUTHOR]

The article discusses a study on the secondary metabolites of endophytic fungus Trichoderma species YM 311505 from Azadirachta indica (A. indica) . It says that Trichoderma sp. YM 311505 fungal strain was isolated from the fruit of A. indica, which was collected in China. It mentions that the crude extracts were subjected to column chromatography on silica gel washed with petroleum-acetone. Results showed that all compounds demonstrated moderate antibacterial and antifungal activities.

Background: To explore the potential correlation between 10-year atherosclerotic cardiovascular disease (ASCVD) risk and depressive symptoms in a general population. Methods: A cross-sectional study involving 11,956 permanent residents of Liaoning Province in China ≥35 years of age was conducted. Depressive symptoms were assessed with the Patient Health Questionnaire-9 (PHQ-9) while 10-year ASCVD risk was calculated using the tool suitable for China. Results: Males had significantly higher 10-year ASCVD risk than females (14.2 ± 10.7% vs. 9.3 ± 9.1%; P < 0.001) but lower PHQ-9 score (2.34 ± 3.13 vs. 3.63 ± 4.02; P < 0.001). The mean PHQ-9 score increased significantly with advancing 10-year ASCVD risk category in both males (from 2.03 to 2.61; P for trend < 0.001) and females (from 3.04 to 4.61; P for trend < 0.001), and the increasing trend was more apparent in females (P < 0.001). Pearson correlation analyses showed that 10-year ASCVD risk positively correlated with PHQ-9 score in both sexes (Ps < 0.001). In multivariate linear regression analyses adjusting for confounding risk factors, the independent associations of 10-year ASCVD risk with PHQ-9 score were all significant in the total (β = 2.61; P < 0.001), male (β = 1.64; P = 0.001), and female subjects (β = 3.71; P < 0.001). Further, the interaction analysis proved the impacts of 10-year ASCVD risk on PHQ-9 score were more apparent in females than males (Ps < 0.001). Conclusions: The 10-year ASCVD risk was positively associated with depressive symptoms in both males and females, which was more apparent in the latter. These findings provided some novel data about the value of 10-year ASCVD risk in estimating depressive symptoms. [ABSTRACT FROM AUTHOR]

Previous studies showed that exoelectrogenic bacteria in paddy soil could suppress methanogens and methanogenesis after they were enriched by application of Fe3+ or running microbial fuel cells (MFCs). However, the relationship between exoelectrogenic bacteria and methanogens without the enrichment process is unknown. Our study was conducted in three paddy fields in China and over three seasons. We explored novel MFC-based sensors to in situ detect voltage signals that were generated from paddy soil within 10 min. The voltage and methane emission flux were determined as an indicator of the exoelectrogenic activity and methanogenic activity, respectively. The abundance of exoelectrogenic bacteria was assessed by quantifying five exoelectrogenic bacterial-associated genera including Geobacter, Shewanella, Anaeromyxobacter, Desulfovibrio and Clostridium, while the methanogens were studied by quantifying and sequencing the mcrA gene. The results showed that the abundance of exoelectrogenic bacteria and the voltage signals were positively correlated to the abundance of mcrA gene and methane emission flux, respectively. Moreover, non-metric dimensional scaling reveals that the abundance of Geobacter, Desulfovibrio and Clostridium significantly correlated with that of Methanomassiliicoccus, Methanoregula and Methanolinea. The present study suggests that the voltage signals might act as a novel indicator of methane emission flux in paddy fields. [ABSTRACT FROM AUTHOR]

Abstract: Aims: To correlate the pre-treatment plasma Epstein–Barr virus (EBV) DNA with tumour burden and to explore the prognostic implications of pre- and post-treatment plasma EBV DNA load in nasopharyngeal carcinoma patients treated with radiotherapy. Materials and methods: Plasma EBV DNA load was measured using a real-time quantitative polymerase chain reaction assay in 69 patients with nasopharyngeal carcinoma before and after radiation treatment and correlated with tumour volume and treatment outcome. Tumour volume was calculated by multiplying the sum of the areas of gross extent of the primary tumour and regional lymph nodes shown by computed tomography images and/or magnetic resonance imaging. Prognostic models for distant metastasis and overall survival were constructed using a multivariable fractional polynomial algorithm. Results: The pre-treatment plasma EBV DNA concentration was significantly associated with tumour volume (Spearman correlation coefficient, 0.61; P <0.001). The multivariable fractional polynomial algorithm selected post-treatment EBV DNA and administration of chemotherapy as prognostic factors for distant metastasis (P <0.001, P =0.021, respectively), as well as for overall survival (P <0.001, P =0.018, respectively). Conclusions: Pre- and post-treatment plasma EBV DNA load have important clinical significance. Pre-treatment plasma EBV DNA concentration reflects tumour burden, whereas clearance of circulating plasma EBV DNA after treatment predicts the risk of distant metastasis and overall survival. [Copyright &y& Elsevier]

Patients with the genotype of β 0 /β 0 for β-thalassemia (β-thal) usually behave as β-thal major (β-TM) phenotype which is transfusion-dependent. The pathophysiology of β-thal is the imbalance between α/β-globin chains. The degree of α/β-globin imbalance can be reduced by the more effective synthesis of γ-globin chains, and increased Hb F levels, modifying clinical severity of β-TM. We report a Chinese child who had homozygous β 0 -thal and a heterozygous KLF1 mutation. The patient had a moderate anemia since 6 months old, keeping a baseline Hb value of 8.0-9.0 g/dL. She had normal development except for a short stature (3rd percentile) until 6 years old, when splenomegaly and facial bone deformities occurred. Although genetic alteration of KLF1 expression in β 0 /β 0 patients can result in some degree of disease alleviation, our case shows that it is insufficient to ameliorate satisfactorily the presentation. This point should be borne in mind for physicians who provide the genetic counseling and prenatal diagnosis to at-risk families.

Han Chinese, Korean and Japanese are the main populations of East Asia, and Han Chinese presents a gradient admixture from north to south. There are differences among the East Asian populations in genetic structure. To achieve fine-scale genetic classification of southern (S-) and northern (N-) Han Chinese, Korean and Japanese individuals in this study, we collected and analyzed 1185 ancestry informative SNPs (AISNPs) from previous literature reports and our laboratory findings. First, two machine learning algorithms, softmax and randomForest, were used to build genetic classification models. Then, phylogenetic tree, STRUCTURE and principal component analysis were used to evaluate the performance of classification for different AISNP panels. The 234-AISNP panel achieved a fine-scale differentiation among the target populations in four classification schemes. The accuracy of the softmax model was 92%, which realized the accurate classification of the S-Han, N-Han, Korean and Japanese individuals. The two machine learning models tested in this study provided important references for the high-resolution discrimination of close-range populations and will be useful tools to optimize marker panels for developing forensic DNA ancestry inference systems.

Multiple sclerosis (MS) is an autoimmune disease that leads to the demyelination of nerve axons. An increasing number of studies suggest that patients with MS exhibit altered metabolic profiles, which might contribute to the course of MS. However, the alteration of metabolic profiles in Chinese patients with MS and their potential roles in regulating the immune system remain elusive. In this study, we performed a global untargeted metabolomics approach in plasma samples from 22 MS-affected Chinese patients and 21 healthy subjects. A total of 42 differentially abundant metabolites (DAMs) belonging to amino acids, lipids, and carbohydrates were identified in the plasma of MS patients and compared with those in healthy controls. We observed an evident reduction in the levels of amino acids, such as L-tyrosine, L-isoleucine, and L-tryptophan, whereas there was a great increase in the levels of L-glutamic acid and L-valine in MS-affected patients. The levels of lipid and carbohydrate metabolites, such as sphingosine 1-phosphate and myo-inositol, were also reduced in patients with MS. In addition, the concentrations of proinflammatory cytokines, such as IL-17 and TNF-α, were significantly increased, whereas those of several anti-inflammatory cytokines and chemokines, such as IL-1ra, IL-7, and MIP-1α, were distinctly reduced in the plasma of MS patients compared with those in healthy subjects. Interestingly, some DAMs, such as L-tryptophan and sphingosine 1-phosphate, showed an evident negative correlation with changes in the level of TNF-α and IL-17, while tightly positively correlating with altered concentrations of anti-inflammatory cytokines and chemokines, such as MIP-1α and RANTES. Our results revealed that altered metabolomic profiles might contribute to the pathogenesis and course of MS disease by modulating immuno-inflammatory responses in the peripheral system, which is essential for eliciting autoimmune responses in the central nervous system, thus resulting in the progression of MS. This study provides potential clues for developing therapeutic strategies for MS in the near future., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Yang, Wu, Ling, Chao, Zhang, Yan, He, Yu and Zhao.)

This retrospective study aimed at identifying the predictors of oxygen desaturation (OD) (i.e., SpO2 < 95%) in patients with obstructive sleep apnea (OSA) requiring deep sedation and developing an algorithm to predict OD.We studied 66 OSA patients undergoing propofol-induced deep sedation for drug-induced sleep endoscopy (DISE). The patients were divided into prediction (n = 35) and validation (n = 31) groups. Patient characteristics and polysomnographic parameters were analyzed with receiver operating characteristic curve and Chi-squared test to identify significant predictors of OD for developing an algorithm in the prediction group. The predictive accuracy, sensitivity, positive predictive value, and negative predictive value of the algorithm were determined in the validation group.Six polysomnographic predictors of OD were identified, including Apnea-Hypopnea Index of total sleep time (AHI-TST), AHI at the stage of rapid eye movement (AHI-REM), percentage of time with oxygen saturation <90% (mO2 < 90%), average SpO2, lowest SpO2, and desaturation index. Stepwise multiple logistic regression analysis demonstrated that low average SpO2 (<95.05%) and high AHI-REM (>16.5 events/h) were independent predictors of OD. The algorithm thus developed showed that patients with an average SpO2 < 95.05% and those with an average SpO2 ≥ 95.05% together with an AHI-REM > 16.5 events/h would be at risk of OD under sedation. The predictive accuracy, sensitivity, positive predictive value, and negative predictive value were 84%, 100%, 83%, 100%, respectively.For patients with OSA, average SpO2 and AHI-REM may enable clinicians to predict the occurrence of oxygen desaturation under deep sedation. Future large-scale studies are needed to validate the findings.

Objective: To investigate maternal and neonatal outcomes after different intrapartum interventions for vaginal birth after cesarean section (VBAC) in mainland China., Methods: A retrospective study was performed on 143 VBAC cases from Beijing Obstetrics and Gynecology Hospital between January 2015 and November 2016. These cases were divided into two groups on the basis of different intrapartum interventions. Maternal and neonatal outcomes were compared., Results: The durations of the first stage and total labor after oxytocin were significantly longer than those before oxytocin use. The proportion of operative vaginal delivery with oxytocin was significantly higher than that without oxytocin (43.9% vs. 11.8%). The times of the first stage, second stage, and total labor with analgesia were significantly longer than those without analgesia (548.4±198.1 vs. 341.8±233.0 minutes, 52.0±38.9 vs. 36.0± 29.1 minutes, and 606.3±212.1 vs. 387.3±233.0 minutes, respectively). Postpartum hemorrhage and operative vaginal delivery occurred significantly more frequently in women with epidural analgesia than in those without epidural analgesia (29.7% vs. 12.3 and 35.1% vs. 16.0%, respectively)., Conclusions: Induction can increase the rate of operative vaginal delivery in VBAC. Oxytocin and epidural analgesia may increase the risk of operative vaginal delivery, and may be associated with a prolonged duration of labor.

Background: Lymphorrhea is probably the most appalling form of lymphedema and is difficult to treat. Intractable lymphorrhea is prone to infection because of skin breakdown. It is believed that supermicrosurgical lymphaticovenous anastomosis is unsuitable for treating such severe disease. Only a few lymphorrhea patients treated with lymphaticovenous anastomosis have been reported. Whether it can be used to treat lymphorrhea has remained inconclusive., Methods: From September of 2015 to June of 2018, 105 patients underwent supermicrosurgical lymphaticovenous anastomosis (n = 746) in the authors' hospital. These patients are divided into the nonlymphorrhea group (three male and seven female patients) and the nonlymphedema group (lymphedema patients without lymphorrhea) (11 male and 84 female patients). Retrospective chart review with demographic data and intraoperative findings were recorded and analyzed. Post-lymphaticovenous anastomosis outcomes for lymphorrhea patients were also recorded., Results: No significant differences were found in patient age, sex, or affected limbs between these two groups. As for intraoperative findings, no differences were found in the percentage of indocyanine green-enhanced lymphatic vessels (52.7 ± 41.1 percent versus 67.3 ± 36.7 percent; p = 0.227) or the pathologic changes of lymphatic vessels based on the normal, ectasis, contraction, and sclerosis type classification (2.2 ± 1.0 versus 2.1 ± 1.0; p = 0.893) between the lymphorrhea and nonlymphorrhea groups, respectively. The average follow-up period was 14.5 months (range, 3 to 31 months). Five lymphorrhea patients (50 percent) showed complete recovery without relapse; significant lymphorrhea reduction was found in three patients (30 percent), and two patients showed minimal improvements (20 percent)., Conclusion: With comparable functional lymphatic vessels identified in lymphorrhea patients, supermicrosurgical lymphaticovenous anastomosis is a viable option for lymphorrhea treatment, with satisfactory results., Clinical Question/level of Evidence: Therapeutic, IV.

Aims: We performed the current study primarily to characterize the independent association of blood pressure with heart rate-corrected QT (QTc) interval after adjusting for cardiovascular confounding factors and left ventricular mass (LVM) in a large general population in China., Methods: All enrolled 10,553 permanent residents with age ≥ 35 years from Liaoning Province were investigated by a questionnaire and then subjected to physical examinations, laboratory analyses, and electrocardiogram (ECG) as well as echocardiogram at the same visit. Multivariate linear and logistic regression analyses were conducted to assess the independent association of blood pressure with QTc interval., Results: Hypertensive subjects had significantly longer QTc interval and higher prevalence of prolonged QTc interval compared with normotensive ones in all subgroups stratified by gender and left ventricular hypertrophy (LVH) (all P s ≤ 0.001). Multiple relevant clinical confounding factors and LVM were all adjusted in the multivariate linear and logistic regression analyses. As a result, both systolic blood pressure (SBP) and diastolic blood pressure (DBP) were independently associated with QTc interval ( β = 0.12 and 0.16, respectively; P s < 0.001). Furthermore, as categorical variables, hypertension was independently associated with prolonged QTc interval (OR = 1.71; P < 0.001). Sex-specific analyses revealed that the independent associations were detected in both males and females (all P s < 0.001)., Conclusions: These key findings of the current study highlighted the fact that hypertension was significantly associated with prolonged QTc interval and the correlations were independent of confounding factors and LVM.

Argasid ticks (Acari: Argasidae) carry and transmit a variety of pathogens of animals and humans, including viruses, bacteria and parasites. There are several studies reporting ixodid ticks (Acari: Ixodidae) and associated tick-borne pathogens in Xinjiang, China. However, little is known about the argasid ticks and argasid tick-associated pathogens in this area. In this study, a total of 3829 adult argasid ticks infesting livestock were collected at 12 sampling sites of 10 counties in the Peripheral Oases, which carry 90% of the livestock and humans population, around the Tarim Basin (southern Xinjiang) from 2013 to 2016. Tick specimens were identified to two species from different genera by morphology and sequences of mitochondrial 16S rRNA and 12S rRNA were derived to confirm the species designation. The results showed that the dominant argasid ticks infesting livestock in southern Xinjiang were Ornithodoros lahorensis (87.86%, 3364/3829). Ornithodoros lahorensis was distributed widely and were collected from 10 counties of southern Xinjiang. Argas japonicus was collected from Xinjiang for the first time. In addition, we screened these ticks for tick-associated pathogens and showed the presence of DNA sequences of Rickettsia spp. of Spotted fever group and Anaplasma spp. in the argasid ticks. This finding suggests the potential role for Argas japonicus as a vector of pathogens to livestock and humans., Competing Interests: The authors have declared that no competing interests exist.

Nine oxygenated guaiane-type sesquiterpenes and three isopimarane diterpenes were isolated from the culture broth of an endophytic fungus, Xylaria sp. YM 311647, obtained from Azadirachta indica. The structures of these compounds were elucidated by interpretation of spectroscopic data. The absolute configurations of two of these were confirmed by X-ray crystallographic analysis. All of the compounds were tested for their antifungal activities against five pathogenic fungal cells. The results showed that nine sesquiterpenes were moderately active against Candida albicans and Hormodendrum compactum with MIC values ranging from 32 to 256μg/ml, while the diterpenes were more active; One of these exhibited the most potent inhibitory activity against C. albicans and Pyricularia oryzae with MIC values of 16μg/ml., (Copyright © 2014 Elsevier Ltd. All rights reserved.)

The Three Rivers Source Area is the largest ecological function region of water source supply and conservation in China. As affected by a variety of driving factors, the ecosystems in this region are seriously degraded, giving definite impacts on the water source supply service. This paper approached the variation patterns of precipitation and runoff coefficient from 1981 to 2010, quantitatively estimated the water source supply of the ecosystems in the region from 1980 to 2005 based on InVEST model, and analyzed the spatiotemporal variation pattern and its causes of the water source supply in different periods. In 1981-2010, the precipitation in the Three Rivers Source Area had a trend of increase after an initial decrease, while the precipitation runoff coefficient presented an obvious decreasing trend, suggesting a reduced capability of runoff water source supply of this region. The potential evapotranspiration had a declining trend, but not obvious, with a rate of -0.226 mm x a(-1). In 1980-2005, the water source supply of the region represented an overall decreasing trend, which was most obvious in the Yellow River Source Area. The spatiotemporal variation of the water source supply in the Three Rivers Source Area was the results of the combined effects of climate and land use change, and the climate factors affected the water source supply mainly through affecting the precipitation and potential evapotranspiration. Climate and land use change induced the ecosystem degradation and underlying surface change, which could be the main driving forces of the declined water source supply in the Three Rivers Source Area.

Background/aims: To assess the incidence of gastric cancer development in gastric benign ulcer patients and to evaluate the value of biopsy by taking specimens from both the base and edges of ulcers in contrast to the traditional biopsy which takes specimens from the edges of ulcers only., Methodology: An endoscopic followup of more than 1 year was conducted on 456 gastric ulcer patients in our hospital for a duration over 8 years. We collected clinical, endoscopic and pathological data and obtained at least 6 biopsies from both the edges and the bases of ulcers healing or complete healing, respectively and assessed H. pylori infection., Results: Gastric cancers developed in 11 (2.41%) of 456 GU patients. In the experimental group, 3 cases that were diagnosed by histology showed adenocarcinoma with specimens taken from the ulcer bases and in the other 5 cases the specimens were taken from the ulcer edges. The detection rate of gastric cancer from gastric ulcer between experimental group and control group was statistically significant (4.57% vs. 1.07%, p<0.05)., Conclusions: Gastric ulcer may develop into gastric cancer over a certain period of time in patients infected with H. pylori. Biopsies from ulcer bases and edges at the second or subsequent endoscopies may lead to defection of gastric cancer earlier and more effectively than the biopsies which take specimens from the edges of ulcers only.

Background: Despite recent reports on the molecular epidemiology of cryptococcal infections in China, clinical isolates have been mostly reported from human immunodeficiency virus (HIV)-negative patients, and environmental isolates from China have rarely been included. The aim of this study was to investigate the ecological profile of Cryptococcus (C.) neoformans and C. gattii in China., Methods: A survey was performed in 10 cities from 20°N (North latitude) to 50°N and in a Eucalyptus (E.) camaldulensis forestry farm at the Guixi forestry center, China., Results: Six hundred and twenty samples of pigeon droppings from 10 cities and 819 E. camaldulensis tree samples were collected and inoculated on caffeic acid cornmeal agar (CACA). The brown-colored colonies were recultured to observe their morphology, growth on canavanine-glycine-bromothymol-blue (CGB) medium, phenol oxidase and urease activities, serotype and mating type. There were obvious differences in the positive sample rates of C. neoformans in pigeon droppings collected from the different cities, ranging from 50% in the cities located at latitudes from 30°N - 40°N, 29% at 20°N - 30°N and 13% at 40°N - 50°N., Conclusions: There were no differences in positive bevy rates (approximately 80%) among the three grouped cities. Mycological tests of 101 isolates purified from pigeon droppings revealed that they were C. neoformans var. grubii. We also observed variable capsular size around the C. neoformans cells in colonies with variable melanin production and the bio-adhesion of the natural C. neoformans cells with other microorganisms. One urease-negative C. neoformans isolate was isolated from pigeon droppings in Jinan city. No C. gattii was isolated in this study.

Based on the tree ring samples of Pinus koraiensis collected from the low altitude areas of Changbai Mountains, seven standard chronologies for tree ring width and cell scale parameters were constructed. Parts of the chronologies were chosen for the correlation analysis with the climate elements in 1959-2007 at Donggang meteorological station, and the changes of the correlations between tree ring indices and climate elements before and after 1988 in which the climate changed abruptly were discussed. In the seven standard chronologies constructed, cell number had the best correlation with tree ring width. Both precipitation and air temperature were the limiting factors for the growth of P. koraiensis, but the chronologies had better correlation with precipitation than with air temperature, mainly manifested in the significant correlation between the chronologies and the precipitation in previous September and current May and June. Comparing with tree ring width, cell size could reveal more climatic information, mainly manifested in the positive correlation between the chronologies and the air temperature in March and the precipitation in May, and the negative correlation between the chronologies and the air temperature in May. After the abrupt change of air temperature in 1988, the responses of cell size to climate elements had some changes, mainly manifested in the decreasing sensitivity to monthly climate elements and the earlier response time.

The prevalence of Perkinsus sp. infection in Manila clam Ruditapes philippinarum was investigated in the coastal areas of east China. Thirteen groups of clams were collected from 5 sites: Dandong and Qingdao Bays (Yellow Sea), Weifang Bay (Bohai Sea), and Ningbo and Fuzhou Bays (East China Sea). The clams were tested for perkinsosis infection using Ray's fluid thioglycollate medium culture assay. Perkinsus sp. was found in samples from all 5 sites from May 2008 to May 2009. Infection prevalence ranged from 43.75 to 95.83%, and was significantly higher in October than in May. The only 3 uninfected groups of clams were collected from Weifang Bay, the site farthest from the ocean. There was no difference in the prevalence of infection among the remaining 4 sites. The conserved internal transcribed spacer regions of the ribosomal RNA gene complex in each of the Perkinsus sp. isolates were amplified by PCR. The resulting amplicons were sequenced and phylogenetically analyzed. All the Perkinsus isolates were identified as Perkinsus olseni.

Based on the widely used Lund-Potsdam-Jena Dynamic Global Vegetation Model (LPJ) for climate change study, and according to the features of natural environment in China, the operation mechanism of the model was adjusted, and the parameters were modified. With the modified LPJ model and taking 1961-1990 as baseline period, the responses of natural vegetation net primary productivity (NPP) in China to climate change in 1991-2080 were simulated under the Special Report on Emissions Scenarios (SRES) B2 scenario. In 1961-1990, the total NPP of natural vegetation in China was about 3.06 Pg C a(-1); in 1961-2080, the total NPP showed a fluctuant decreasing trend, with an accelerated decreasing rate. Under the condition of slight precipitation change, the increase of mean air temperature would have definite adverse impact on the NPP. Spatially, the NPP decreased from southeast coast to northwest inland, and this pattern would have less variation under climate change. In eastern China with higher NPP, especially in Northeast China, east of North China, and Loess Plateau, the NPP would mainly have a decreasing trend; while in western China with lower NPP, especially in the Tibetan Plateau and Tarim Basin, the NPP would be increased. With the intensive climate change, such a variation trend of NPP would be more obvious.

Based on the China 1:1000000 vegetation type map, and by using GIS spatial analysis, the spatial pattern of major vegetation landscape diversity indices and its relationships with environmental factors in Longitudinal Range-Gorge Region (LRGR) were analyzed. The proper scale for studying the vegetation landscape diversity in LRGR was 2000 m. In the study region, an obvious regional difference was observed in the vegetation landscape diversity indices, exhibiting typical longitudinal "corridor" and latitudinal "barrier" characteristics. The correlations between the vegetation landscape diversity indices and environmental elements were significant, and the regional difference in the environmental elements was the main factor controlling the spatial pattern of vegetation landscape diversity indices. The "corridor-barrier" function of the longitudinal range-gorge terrain made a spatial redistribution of hydro-thermal conditions, being the main cause of the special pattern of the vegetation landscape diversity in LRGR.

Objective: Since the advent in 2004 of highly active antiretroviral therapy (HAART) in Liaoning, a dramatic improvement had been seen in the number of patients attaining undetectable viral loads (92/104), but the extent of mutation diversity on human immunodeficiency virus 1 (HIV-1) and the prevalence of drug resistance had remained elusive. This study aimed to analyze both HIV-1 mutation profiles and prevalence related to antiretroviral resistance following therapeutic failure., Methods: A total of 104 blood samples circling Liaoning from HAART-treated between 2004 and 2008 were studied. Patients' CD(4)(+) T-cell count and viral load were determined. HIV-1 pol (PR and part of RT) gene fragments were amplified from patients' plasma by reverse transcriptase polymerase chain reaction (RT-PCR) and nest-PCR, subsequently sequenced and analyzed., Results: CD(4)(+) T cell numbers and viral replication capacity were assessed. 88.4% (92/104) of the patients were successful after initial non-suppressive NRTI & NNRTI-based HAART regimens. Subjects on non-nucleoside reverse transcriptase inhibitor (NNRTI) regimens developed more (6/104) drug-resistance mutations than those on nucleoside reverse transcriptase inhibitor (NRTI) regimens did (5/104). No protease-inhibitor (PI) drug resistance mutations developed. The whole rate of drug resistance mutations was about 6.73%. Subjects developing NNRTI-resistance (NNRTI-R) seemed more likely to develop drug-resistant viremia than with NRTI-based HAART., Conclusion: This finding might have implications in which that the prevalence of drug-resistance mutations was low but remained risk of transmission in HIV-infected therapeutic failure. Meanwhile, data from the present study showed that there was a high frequency of primary mutations, which offered resistance to nrti and nnrti. Monitoring patients with treatment failure seems an important tool in helping the physicians to improve their treatment schedule and to carry out epidemiological surveillance programs.

This study aims to investigate the expression and significance of EphA2 and EphrinA-1 in human gastric adenocarcinoma progression and prognosis. The expression of EphA2 and EphrinA-1 was detected in the cell lines and tissues of gastric adenocarcinoma. Different expression levels of EphA2 and EphrinA-1 were found in two cell lines. The expression of EphA2 and EphrinA-1 was significantly higher in gastric adenocarcinoma tissues than in normal tissues. Statistical analysis showed a significant correlation of EphA2 expression with the depth of tumor invasion, tumor-node-metastasis (TNM) stages, and lymph node metastasis. EphrinA-1 over-expression was significantly correlated with TNM stages and lymph node metastasis, while EphA2 expression was found to be an independent prognostic factor of postoperative gastric adenocarcinoma. In conclusion, the increased expression of EphA2 and EphrinA-1 plays an important role in the progression of human gastric adenocarcinoma, in which elevated EphA2 expression is an independent factor that indicates poor prognosis in postoperative gastric adenocarcinoma.

Objective: To investigate the HIV-1 drug resistance associated mutations and examine the susceptibility of HIV-1 with these mutations to antiretroviral in treatment-naive individuals in Liaoning province from 2004 to 2008., Methods: RNA was extracted from 20 plasma samples of diagnosed untreated HIV-1-infected treatment-naive patients by drawing method. After the viral loading (VL) test, the protease and nucleoside reverse transcriptase coding regions were amplified by RT-PCR, nested PCR and sequence analysis directly. Levels of resistance and prevalence were evaluated according to the Stanford University HIV Drug Resistance Database's algorithm (http://hivdb.stanford.edu)., Results: Among the 20 plasma samples, 13 got PCR products because of their VL values higher than 1000 copies/ml.Meanwhile, the 13 samples got 65 sequences by using 5 primers each. Polymorphisms in subtype H and circulating recombinant forms (CRFs) CRF10&#95;CD sequences were identified. An overall prevalence of 30.8% (4/13) resistance to NNRTIs, 7.7% (1/13) to PI and no NRTIs mutations were found. The most frequent substitutions (4/13) in the RT region at positions P225H, K238S, V179D, K238T and a major position I54S in PR implied to a multiple drug-resistance. A71V or L10V only, respectively, substitution in PR was found in 3 samples, but no any worse with drug sensitivity., Conclusion: HIV-1 polymorphisms in subtype H and CRFs CRF10&#95;CD sequences were identified circulating in Liaoning. A major mutation position I54S in PR implied that it would be the time to commence a higher level drug regimen.

Objective: To understand the HA1 genetic variation characterization of influenza virus subtype H3N2 circulated from 2001 to 2006 in Liaoning local area., Methods: Viral RNA was extracted and transcribed into cDNA by reverse transcriptase and amplified by PCR. The product of PCR was purified by QIAgen purification kits,and sequenced by ABI 3100avant. The sequence data were analyzed phylogenetically by Sequence software with epidemic records. Finally, the phylogenetic trees were drawn according to deduced amino acid sequences of influenza virus H3N2 from 2000 to 2006 in the NCBI database., Results: The seven HA1domain sequences of H3N2 influenza viruses circulated from 2001 to 2006 in Liaoning local area had been analyzed. Compared with WHO 2004-2006 H3N2 vaccine A/California/7/2004, 12 bases had changed, 4 positions had amino acid substitution in 62 * > E, 182 T > 1,224 S > A,225 C > Y. 224 and 225 are RBS (Receptor binding site). The homology is lower than 98%. Phylogenetic tree showed Liaoning H3N2 2006 strains and Zhejiang 2005 strains were similar to WHO Northern hemisphere winter 2006-2007 Vaccine A/Wisconsin/67/2005 (H3N2)-like virus and grouped together to form an independent cluster even though several bases were still different., Conclusion: The HA1 domain of HA gene of influenza viruses (H3N2) isolated from 2001-2006 in Liaoning local area showed base mutation, amino acid sequence difference compared to A/California/7/2004 (2005-2006 vaccine), suggesting it might be the main cause leading to the spread of influenza. The sequence analysis showed Liaoning 2006 H3N2 strains were similar to those from Southern area which suggested that further surveullance should be conducted to monitor the virus mutation in circulation.

Based on the 1966-2005 observation data from 113 meteorological stations on the Tibetan Plateau and its surrounding areas, the spatial patterns of mean temperature of warmest month (TWM), mean temperature of coldest month (TCM), duration of mean daily temperature > or = 10 degrees C (DT10), annual precipitation (AP), mean annual potential evapotranspiration (PE), and annual arid index (AI), on the Plateau were analyzed. It was shown that the TWM was 7-20 degrees C, which decreased from eastern surrounding area to the central region, while TCM was--18.4 degrees C to 8 degrees C, being decreased from south to north. The DT10 decreased from eastern surrounding area ( > 150 days) to central region (50 days), and the AP decreased from southeast to northwest. The PE calculated by Thornthwaite model decreased from southeast to northwest, and was 330-750 mm in most parts of the study area. Arid area occupied a larger proportion and mainly located in the northwest of Tibetan Plateau, while humid area was smaller and mainly located in the east and southeast of the Plateau. The vegetation distribution on Tibetan Plateau had good correlations with the test eco-climatic factors, suggesting that these factors could better characterize the vegetation patterns on Tibetan Plateau.

The chemical investigation of the crude extract of the marine-derived Streptomyces sp. M491 yielded three new sesquiterpenes, namely, 10alpha,11-dihydroxyamorph-4-ene (4), 10alpha,15-dihydroxyamorph-4-en-3-one (6), and 5alpha,10alpha,11-trihydroxyamorphan-3-one (7). In addition, the known compounds 10alpha-hydroxyamorph-4-en-3-one (2), o-hydroxyacetanilide, genistein, prunetin, and indole-3-carbaldehyde and the macrolide antibiotic chalcomycin A were identified. The structures were determined on the basis of spectroscopic analysis, especially 1D and 2D NMR data. This is the first report of these sesquiterpenes from bacteria.

Objective: From December 1998 to April 2004, 3960 umbilical cord blood units were stored in Guangzhou cord blood bank, which provided 100 umbilical cord blood units to 25 transplant center for 83 patients with malignant or non-malignant diseases. To study the related factors affecting unrelated umbilical cord blood stem cell transplantation, the authors analyzed retrospectively the results of transplantation of unrelated umbilical cord blood stem cells for 65 patients., Methods: ALL (acute lymphocytic leukemia) cord blood units were obtained from full term normal vaginal and cesarean deliveries in Guangzhou Women and Infants Hospital. The fractionation, cryopreservation and thawing of the cord blood were performed according to the regulations of New York umbilical cord blood bank and pertinent literature. The selection of cord blood was based on HLA typing and the number of nucleated cells. The sex and HLA antigens of donors were defined as the evidence of engraftment. Time to engraftment was recorded when the absolute number of neutrophil ANC (absolute neutrophil count) was higher than 5.0 x 10(8) for three days. Event-free survival and graft versus host disease (GVHD) were provided by transplant centers., Results: Out of 65 patients who received unrelated cord blood stem cell transplant, 49 patients were diagnosed as having malignant diseases [including 23 with ALL, 16 with AML (acute myeloid leukemia), 7 with CML (chronic myelogenous leukemia), 3 with lymphoma and one with MDS (myelodysplastic syndrome)], 16 patients had non-malignant disease. The 65 transplanted patients (42 male, 23 female) had a median age of 10 years (range 1 - 33 years) and a median body weight of 27 kg (range 10 - 67 kg). The patients received cord blood stem cells from unrelated 0-locus (n = 9) or 1-locus (n = 43) or 2-locus (n = 13) HLA mismatched donor. The median dose of infused cells was: total neutrophil count (TNC) 5.7 x 10(7), CD(34)(+) 5.1 x 10(5), CFU-GM 3.8 x 10(4). Fifty of 65 (77%) patients had engraftment. GVHD occurred in 41 patients (63%), including acute grade I - II GVHD in 31 patients (76%), acute grade III - IV GVHD in 8 patients (20%) and chronic GVHD in 2 patients (5%). Fifty patients had engraftment (ANC > 5.0 x 10(8)) after a median time of 17 (range 7 to 44) days after transplant, while an autologous hematopoietic reconstitution was observed in 6 patients; 24 patients died of severe pneumonia (n = 8), acute GVHD (n = 4), or sepsis (n = 12) and the disease-free survival probability was 61%., Conclusions: Unrelated allogeneic umbilical cord blood transplantation may be a good substitution for unrelated allogeneic bone marrow transplantation with a good prospect.

Objective: To investigate the prevalence and subtypes of influenza viruses in Liaoning regions from November 1999 to March 2005., Methods: Influenza virus was isolated by embryonated eggs together with cell culture and subtypes, identified by HI test., Results: During the study in 1999 - 2005, a total number of 2713 swab specimens were collected in different cities in Liaoning regions in which 188 strains were identified for influenza viruses with an average rate as 7.0%. A total number of 1466 swab specimens were collected by both Centers for Disease Control and Prevention in Dalian city and Liaoning province, and 167 strains were identified positive with an average rate of 11.4%. Influenza A3, A1 and B/Yamagata all appeared before March 2002 which were predominant strains. However, since then Influenza A1 has never appeared again in Liaoning regions and B showed some changes, from Yamagata to Victoria, the characteristics on the prevalence of influenza appeared only in the period of November to February., Conclusion: It was meaningful to analyze the surveillance data of influenza in different years in Liaoning regions in order to better understand the characteristics of influenza and the shifting of subtype.

To study the program of evaluating mothers and infants after 6 months of cord blood donation, from June 1998 to February 2004, all mothers after 6 months of cord blood donation were followed-up by phone calls or letters to report on the health condition. The results showed that when 3 195 mothers were visited by phone calls, 18 mothers declined to answer. 392 letter were send to those who could not be found by phone, 15 of whom wrote back. The average time to talk with each mother was approximately 12 minutes. Follow-up on the baby donors showed two cases with chromosome abnormality, one with hypothyroidism, one with neutropenia, one with albinism and 5 dead with unclear reasons. The cord blood components from all these abnormal donors found were discarded. In conclusion, the programs to evaluate mother and baby after 6 months of cord blood donation seems important in quality control of the components stored in cord blood bank.

In order to research the related factors of umbilical cord blood transplantation, 54 cases of unrelated umbilical cord blood transplantation were analyzed retrospectively, which were performed from June 1998 to July 2003. All cord blood units were obtained from full term normal vaginal deliveries in Guangzhou Maternal-Neonatal Hospital. The fractionation, cryopreservation and thawing of cord blood have been done according to the regulation of New York umbilical cord blood bank and pertinent literature. The selection of cord blood is based on HLA typing and the number of nucleated cells. The results showed that from June 1998 to July 2003, 3 475 units of cord blood were collected in Guangzhou Umbilical Cord Blood Bank and 99 units were provided for therapy of 85 patients in 21 transplantation centers, including 11 sibling and 74 unrelated cord blood transplantations. 54 cases of unrelated cord blood transplantation were reported, including 43 malignant diseases and 11 non-malignant diseases. The median age of recipients was 9.5 (range 1.2 - 33) years, the median weight was 27 (range 10 - 60) kg, the median number of TNC was 6.82 x 10(7)/kg, 43 cord blood were implanted (ANC > 500/microl) at day 60 after transplantation (79.6%, median 17). The time of nuclear cell reconstitution after cord blood transplantation was statistically related with nucleated cells and the type of disease, not related with HLA matching. Acute GVHD was present in 8 patients (21.6%) and chronic GVHD occurred in 2 patients (5.4%), 6 patients suffered from graft failure (11.1%). The total survival rate was 42.6%. It is suggested that unrelated umbilical cord blood transplantation seems to be a good substitute for bone marrow transplantation and has good prospects especially in children.