Search

Traditional clinical modalities for diagnosing bladder urothelial carcinoma (BUC) remain limited due to their invasive nature, significant costs, discomfort associated with cystoscopy, and low sensitivity to urine cytology. Therefore, there is an urgent need to identify highly sensitive, specific, and noninvasive biomarkers for the early detection of this neoplasm. Hypermethylated TWIST1/Vimentin promoter may be a noninvasive biomarker using urine sample. We assessed the TWIST1/Vimentin promoter methylation status in urine samples using the Methylated Human TWIST1 and Vimentin Gene Detection Kit (Jiangsu MicroDiag Biomedicine Co., Ltd., China). The samples were collected from five groups: group 1 consisted of patients with BUC, group 2 contained other patients with urologic tumors, group 3 consisted of patients with benign diseases (e.g., urinary tract infections, lithiasis, and benign prostatic hyperplasia), Group 4 included UTUC (upper tract urothelial carcinoma) patients and group5 comprised healthy individuals. The study encompassed 77 BUC patients, and we evaluated the degree of methylation of the TWIST1/Vimentin gene in their urine samples. Notably, TWIST1/Vimentin positivity was significantly elevated in comparison to groups 2, 3 and 5 (all p < 0.001) at a rate of 77.9%, but no significant difference was observed when compared to group 4. In the relationship between TWIST1/Vimentin methylation and clinicopathological features of BC patients from our center, we found there was no significant association between TWIST1/Vimentin status and proteinuria and/or hematuria, and hypermethylation of TWIST1 / VIM genes was found in both high and low tumor grade and in both non-muscle invasive bladder cancer (stages Tis, Ta, or T1) and muscle-invasive bladder cancer (stage T2 or above). In the multivariable analysis for cancer detection, a positive TWIST1/Vimentin methylation were significantly linked to a heightened risk of BC. Moreover, TWIST1/Vimentin promoter methylation demonstrated an ability to detect BUC in urine samples with a sensitivity of 78% and a specificity of 83%. Our findings reveal that hypermethylation of the TWIST1/Vimentin promoter occurs in bladder urothelial carcinoma, and its high sensitivity and specificity suggest its potential as a screening and therapeutic biomarker for urothelial carcinoma of the bladder. [ABSTRACT FROM AUTHOR]

International guidelines recommend repeat transurethral resection of bladder tumors (reTURB) for selected patients with high-risk non-muscle invasive bladder cancer to remove possible residual tumors, restage tumors and improve the therapeutic outcome. However, most evidence supporting the benefits of reTURB is from conventional TURB. The role of reTURB in patients receiving initial En bloc resection of bladder tumor (ERBT) is still unknown. PubMed, Embase, Web of Science, The Cochrane Library, and China National Knowledge Infrastructure (CNKI) were systematically searched. Finally, this systematic review and meta-analysis included twelve articles, including 539 patients. The rates of residual tumor and tumor upstaging detected by reTURB after ERBT were 5.9% (95%CI, 2.0%–11.1%) and 0.0% (95%CI, 0.0%–0.5%), respectively. Recurrence-free survival, tumor recurrence and progression were comparable between patients with and without reTURB after initial ERBT. The pooled hazard ratios of 1-year, 2-year, 3-year and 5-year recurrence-free survival were 0.74 (95%CI, 0.36–1.51; p = 0.40), 0.76 (95%CI, 0.45–1.26; p = 0.28), 0.83 (95%CI, 0.53–1.32; p = 0.43) and 0.83 (95%CI, 0.56–1.23; p = 0.36), respectively. The pooled relative risks of recurrence and progression were 0.87 (95%CI, 0.64–1.20; p = 0.40) and 1.11 (95%CI, 0.54–2.32; p = 0.77), respectively. Current evidence demonstrates that reTURB after ERBT for bladder cancer can detect relatively low rates of residual tumor and tumor upstaging and appears not to improve either recurrence or progression. [ABSTRACT FROM AUTHOR]

Plastic pollution has been a growing global issue. Various plastic additives may enter the environment with plastic debris, which could also become contaminants. Lifetime bioaccumulation, gender difference, tissue distribution, and parental transfer potential of commonly applied organophosphorus plastic additives (OPPAs) were investigated in wildlife fish of the Pearl River system, China. The OPPAs were widely detected in 7 consumable fish species. Tris (2-chloropropyl) phosphate was the predominant compound, with a median concentration of 18.8 ng/g lipid weight. The total OPPA concentrations (ΣOPPAs) were higher in the livers and swimming bladders, suggesting important roles of lipophilicity on the OPPAs accumulation in the fish. Besides, the livers were more abundant in the non-chlorinated OPPAs relative to the other tissues, indicating potentially stronger metabolism of the chlorinated OPPAs in the livers. Redbelly tilapia contained obviously lower ΣOPPAs than the other species. On the other hand, proportions of the chlorinated OPPAs were obviously lower in barbel chub and Guangdong black bream. For an individual species, higher ΣOPPAs were usually detected in the female than in the male fish. Furthermore, the females contained higher proportions of the non-chlorinated OPPAs. These results suggested potentially more accumulation of the OPPAs, particularly the non-chlorinated OPPAs in the female than in the male fish. Body weight dependence of the OPPAs accumulation showed varied patterns depending on species, tissue, and compound. Species-specific characteristics affected by both ecology and organisms' physiology should be considered in combination in assessing bioaccumulation of the OPPAs. The OPPAs were slightly bioaccumulative with LogBAFs of 1.2–3.3. The OPPAs did not show obvious inclination to be partitioned to biota from sediment. Omnipresence of the OPPAs in both egg/ovary and testis of the fish suggested potential transgenerational transfer of these chemicals, which can be a serious ecological issue and warrants further research. [Display omitted] • Higher OPPAs levels were usually detected in the female than in the male fish. • Nonchlorinated and chlorinated OPPAs were more abundant in the female and male fish, respectively. • Body weight dependent accumulation of the OPPAs varied by species, tissue, and compound. • The OPPAs were widely present in eggs and testis of the fish. The organophosphorus plastic additives were generally more abundant in the female than the male fish and have potential of paternal transfer. [ABSTRACT FROM AUTHOR]