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To better understand the geodynamic evolution of northeastern China during the Late Mesozoic, we analyzed zircon U–Pb geochronological, Lu–Hf isotopic, and geochemical data for Early Cretaceous volcanic rocks from the southeastern margin of the Songliao Basin. Newly identified A-type rhyolite and trachyandesite yielded zircon 206Pb/238U ages of ca. 123 Ma and 117 Ma, respectively. The rhyolites are high in SiO2 (72.24–78.89 wt%) and total alkali (K2O + Na2O = 8.81–10.03 wt%), and low in MgO (0.10–0.26 wt%), CaO (0.32–0.36 wt%), Ni (0.08–2.69 ppm), and Cr (0.39–4.87 ppm) concentrations, with negative Nb, Ta, and Sr anomalies. They are enriched in light rare earth elements (LREEs) and large-ion lithophile elements (LILEs) and depleted in high-field-strength elements (HFSEs); the calculated Zr saturation temperatures are high (828–915 °C). The A-type rhyolites possess variable zircon εHf(t) values ranging from + 5.69 to + 10.49. Petrogenetic analysis leads us to propose that the A-type rhyolites were probably formed by partial melting of a Neoproterozoic–Early Paleozoic juvenile lower crust. The trachyandesites have Nb/Ta (14.9–17.25), Zr/Hf (35.04–42.75), Rb/Sr (0.25–0.40), and Lu/Yb (0.14–0.15) ratios that are similar to those of mantle-derived magma, indicating a mantle source. They have εHf(t) values of + 4.71 to + 7.29 and show enrichment in LILEs and LREEs, and weak depletion in HFSEs, suggesting that the parent magma originated from partial melting of a depleted lithospheric mantle, and was subsequently metasomatized by subduction-related fluids, followed by extensive fractional crystallization during the magma evolution. Combined with the temporal and spatial distribution of Late Mesozoic igneous rocks from the southeastern margin of the Songliao Basin, we propose that Early Cretaceous volcanic rocks formed in an extensional tectonic setting that was closely related to rollback of the Paleo-Pacific (Izanagi) oceanic slab. [ABSTRACT FROM AUTHOR]

Understanding how to effectively stimulate creative potential in the workplace through strategic human resource management (HRM) practices is critical to enhancing organizational competitiveness. This study investigates the effects of high‐involvement work systems (HIWS) on team creativity in terms of ability–motivation–opportunity theory and a team input–process–output model. It draws on a data set consisting of 668 employees from 112 teams in 41 organizations in China. The results reveal that HIWS are positively related to team creativity, not only directly, but also indirectly through a sequential mediating mechanism of involvement climate and shared leadership. We argue that when employees are placed in an autonomous work environment underpinned by HIWS, they will be more likely to be involved in creative activities and engage in mutual influence behaviors in their work teams, and lead each other to achieve a higher level of creativity. Our study extends the knowledge on strategic HRM/HIWS, shared leadership, and creativity. [ABSTRACT FROM AUTHOR]

Epizootic haemorrhagic disease (EHD) is an infectious, non-contagious viral disease of ruminants caused by epizootic haemorrhagic disease virus (EHDV) and is transmitted by insects of the genus Culicoides. In 2008, EHD was listed on the World Organization for Animal Health (WOAH) list of notifiable terrestrial and aquatic animal diseases. This article reviews the distribution of EHD in China and relevant studies and proposes several suggestions for the prevention and control of EHD. There have been reports of positivity for serum antibodies against EHDV-1, EHDV-2, EHDV-5, EHDV-6, EHDV-7, EHDV-8 and EHDV-10 in China. Strains of EHDV-1, -5, -6, -7, -8 and -10 have been isolated, among which the Seg-2, Seg-3 and Seg-6 sequences of serotypes -5, -6, -7 and -10 belong to the eastern topotype. The emergence of western topotype Seg-2 in EHDV-1 strains indicates that EHDV-1 strains in China are reassortant strains of the western and eastern topotypes. A novel serotype strain of EHDV named YNDH/V079/2018 was isolated in 2018. Chinese scholars have successfully expressed the EHDV VP7 protein and developed a variety of ELISA detection methods, including antigen capture ELISA and competitive ELISA. A variety of EHDV nucleic acid detection methods, including RT–PCR and qRT–PCR, have also been developed. LAMP and the liquid chip detection technique are also available. To prevent and control EHD, several suggestions for controlling EHD transmission have been proposed based on the actual situation in China, including controlling the number of Culicoides, reducing contact between Culicoides and hosts, continued monitoring of EHDV and Culicoides in different areas of China and further development and application of basic and pioneering research related to EHD prevention and control. [ABSTRACT FROM AUTHOR]

The organic soil in the Dianchi Lake area contains much humic acid (HA), and the impact of HA on cemented soil has attracted much attention. In this study, test soil is prepared based on the actual HA content of the soil in Dianchi Lake, and cement is added to prepare samples. Unconfined compressive strength (UCS), scanning electron microscopy (SEM), energy-dispersive analysis (EDS), and X-ray diffraction (XRD) tests are conducted on the samples. The strength development and microstructure changes of cemented organic soil are studied. The results show that (1) cemented soil strength decreases with the increase in HA content and increases with the cement mixing ratio; (2) SEM and EDS tests show that with the increase in HA content, the connection between soil particles gradually changed from surface–surface to point–surface, point–edge, or point–point connection. A large overhead structure is inside the cemented soil, and its integrity weakens with increased HA content. However, elements such as Ca, Si, and Al exist in the pores of cemented soil, indicating that cement hydration products are cemented between soil particles. XRD testing shows that with the increase in HA content, the intensity of the diffraction peaks of hydration products in cemented soil gradually decrease. (3) In general, HA will gradually deteriorate the integrity of cemented soil and reduce its strength. When the HA content is more than 15%, the effect of HA on strength is more prominent. However, increasing the cement mixing ratio can effectively weaken HA's negative effect on cemented soil's strength. When the cement mixing ratio is between 15% and 25%, the strength of cemented soil with high HA content can be effectively increased. However, this approach is not conducive to the protection of the environment. [ABSTRACT FROM AUTHOR]

Background: In China, cage systems with a high space utilization have gradually replaced ground litter systems, but the disease incidence of chickens in cages is higher. Broilers in the ground litter pens may be stimulated by more environmental microbes during the growth process and show strong immune function and status, but knowledge of which microbes and their metabolites play an immunomodulatory role is still limited. This study aimed to explore the differences and correlations in the immune function, gut microbiota and metabolites and the importance of gut microbiota of broilers raised in cages and ground litter pens. Methods: The experiment involved a 2 × 2 factorial arrangement, with rearing systems (cages or ground litter pens) and antibiotic treatment (with or without broad-spectrum antibiotics in drinking water) as factors. Results: The results showed that, compared with the cage group, the ground litter broilers had stronger nonspecific immune function (Macrophages% and NO in blood), humoral immune function (IgG in blood, LPS stimulation index in ileum) and cellular immune function (T%, Tc%, ConA stimulation index and cytokines in blood). Antibiotic (ABX) treatment significantly reduced nonspecific immune function (Macrophages% and NO in blood, iNOS and Mucin2 mRNA expression in ileum), humoral immune function (IgG in blood and sIgA in ileum) and cellular immune function (T% and cytokines in blood, Th and Tc ratio, TLRs and cytokines mRNA expression in ileum). Furthermore, the ground litter broilers had higher α diversity of microbiota in ileum. The relative abundance of Staphylococcus, Jeotgalicoccus, Jeotgalibaca and Pediococcus in the ileum of ground litter broilers were higher. ABX treatment significantly reduced the α diversity of ileal microbiota, with less Chloroplast and Mitochondria. In addition, the levels of acetic acid, isobutyric acid, kynurenic acid and allolithocholic acid in the ileum of ground litter broilers were higher. Spearman correlation analysis showed that Jeotgalibaca, Pediococcus, acetic acid, kynurenic acid and allolithocholic acid were related to the immune function. Conclusions: There were more potential pathogens, litter breeding bacteria, short-chain fatty acids, kynurenine, allolithocholic acid and tryptophan metabolites in the ileum of broilers in ground litter pens, which may be the reason for its stronger immune function and status. [ABSTRACT FROM AUTHOR]

In this study, we conducted sandstone petrography analyses and determined detrital zircon U–Pb ages and Hf isotopes for sandstones collected at different stratigraphic levels of the lowest strata of the Laiyang Group. All results nearly point to an evolutionary trend for the source regions of the strata. The detrital zircon U–Pb results reveal multiple age groups at 140–123 Ma, 810–700 Ma, and 2,500–1,750 Ma, but variable age proportions for two sandstones (19LY‐01Z and ‐05Z) collected at lowermost and middle levels of strata, respectively, but only one unimodal age mode at ca. 1,850 Ma for the uppermost sandstone 19LY‐09Z. Provenance analysis illustrates that the lower strata likely include two source regions, including the Jiaobei Terrane and the Sulu orogen, whereas the upper strata appeared to have one dominant source region (i.e., Jiaobei Terrane). Framework modes of sandstones display that lower samples contain more metamorphic lithic fragments, which corresponds to the recycled orogenic field in the Q‐F‐L diagram, while middle‐upper samples are enriched in feldspars but deficient in lithics and plot in the basement uplift field of the Q‐F‐L diagram. This compositional variation reflects an evolution of the source regions from the Sulu Orogen to the Jiaobei Terrane, completely consistent with the observation of an increase in the amount of detrital zircons sourced from the Jiaobei Terrane, but decreasing detrital zircons from the Sulu Orogen in an upward order. This shift in the provenance of sediments reflects two tectonic events including exhumation of the Sulu Orogen to the surface before sedimentation of the Laiyang Group, and a rapid uplift of the Jiaobei Terrane after ca. 125 Ma following removal of lithospheric mantle. [ABSTRACT FROM AUTHOR]

Background: Studies on the 2019 novel coronavirus disease (COVID-19) have generally been limited to the description of the epidemiology and initial clinical characteristics. We investigated the temporal progression in patients with COVID-19.Methods: In this retrospective, single-center study, we included confirmed cases of COVID-19 from Jan 20 to Feb 6, 2020 in Shanghai. Final date of follow-up was February 25, 2020.Results: Of the 249 patients enrolled, the median age was 51 years old, and 126 (50.6%) were male. The duration from onset of symptoms to hospitalization was 4(2-7) days in symptomatic patients. Fever was occurred in 235(94.3%) patients. A total of 215 (86.3%) patients had been discharged after 16(12-20) days hospitalization. The estimated median duration of fever in all the patients with fever was 10 days (95 confidential intervals [CIs]: 8-11 days) after onset of symptoms. Patients who were transferred to intensive care units (ICU) had significantly longer duration of fever as compared to those not in ICU (31 days v.s. 9 days after onset of symptoms, respectively, P <0.0001). Radiological aggravation of initial image was observed in 163 (65.7%) patients on day 7 after onset of symptoms. 154(94.5%) of these patients showed radiological improvement on day 14. The median duration to negative reverse-transcriptase PCR tests of upper respiratory tract samples was 11 days (95 CIs: 10-12 days). Viral clearance was more likely to be delayed in patients in ICU than those not in ICU (P <0.0001). In multivariate logistical analysis, age (Odds ratio [OR] = 1.06) and CD4 T cell count (OR = 0.55 per 100 cells/ul increase) were independently associated with ICU admission.Conclusions: The majority of COVID-19 cases are mild. The clinical progression pattern suggests that early control of viral replication and application of host-directed therapy in later stage is essential to improve the prognosis of CVOID-19. [ABSTRACT FROM AUTHOR]

Purpose: The purpose of this paper is to develop a measurement of creativity-oriented HRM systems that improve organizational creativity. This paper also aims to explore the mechanisms between them by investigating the mediating role of innovative culture and the moderating role of customer orientation. Design/methodology/approach: The study uses a sample of 82 knowledge-intensive companies with 780 respondents consisting of 145 HR professionals, 512 core knowledge workers and 123 top managers in China. Exploratory factor analysis, confirmatory factor analysis and regression analysis are used to validate the measure of creativity-oriented HRM systems and test hypotheses. Findings: This study finds that creativity-oriented HRM systems are composed of three dimensions, which are creative skill-enhancing practices, intrinsic motivation-enhancing practices and empowerment-enhancing practices. These practices significantly improve organizational creativity through innovative culture. Furthermore, customer orientation moderates the effect of innovative culture on organizational creativity in such a way that the positive relationship is stronger when customer orientation is high. Originality/value: This study contributes to the strategic human resource management literature by developing and validating a measure of creativity-oriented HRM systems. Moreover, it also explores the mechanism between creativity-oriented HRM systems and organizational creativity based on a complementary perspective of innovativeness, which underlines the important mediating effect of innovative culture. More importantly, the authors propose the significance of absorbing knowledge and information from customers and put forward the moderating role that customer orientation plays, especially in an emerging country context such as China. [ABSTRACT FROM AUTHOR]

Magmatic and metasomatic rock suites can be commonly found in active convergent margins as the suprasubduction zone mantle wedge is usually impregnated by melts and fluids. Here we present petrologic, whole-rock geochemical and zircon U-Pb and Lu-Hf data from metamorphic intermediate-mafic and ultramafic rocks in central Jilin Province, NE China, to constrain the Early Paleozoic tectonic evolution of the southeastern margin of the Xing'an-Mongolia Orogenic Belt. Lithologically, the meta-igneous rocks are composed of plagioclase-actinolite, meta-allgovite, tremolitized pyroxenite, and serpentinized peridotite. Geochronological results indicate that the protoliths of the meta-igneous rocks formed during the Early–Middle Ordovician (474–466 Ma). Geochemical features suggest that the rocks are calc-alkaline and transitional-series rocks relatively enriched in large-ion lithophile and light rare earth elements and depleted in high field strength and heavy rare earth elements, implying that they formed in a supra-subduction zone environment. Additionally, the protoliths of the plagioclase-actinolites, which display an affinity for sanukites, resulted from an equilibrium reaction between mantle peridotite and slab melts derived from the partial melting of subducting sediments during early-stage subduction; the meta-allgovite rocks were likely derived from a juvenile depleted lithospheric mantle that was metasomatized by subduction-related fluids; the ultramafic rocks are magmatic cumulates formed in a suprasubduction zone mantle wedge. Furthermore, the meta-allgovite rocks display both arc-like and MORB-like geochemical characteristics, suggesting an intra-continental back-arc basin setting. These features, combined with the presence of the abundance of zircon xenocrysts with Neoproterozoic–Paleozoic ages, as well as the results of previous studies, reflect that a small-scale and limited back-arc basin, which was related to the northward subduction of the Paleo-Asian oceanic plate, probably existed close to the Songnen-Zhangguangcai Range Massif during the Early–Middle Ordovician. [ABSTRACT FROM AUTHOR]

We report zircon U-Pb geochronologic and geochemical data for the post-collisional volcanic rocks from the Batamayineishan (BS) Formation in the Shuangjingzi area, northwestern China. The zircon U-Pb ages of seven volcanic samples from the BS Formation show that the magmatic activity in the study area occurred during 342-304Ma in the Carboniferous. The ages also indicate that the Palaeo-KaramailiOcean had already closed by 342Ma. Moreover, the volcanic rocks also contained 10 inherited zirconswith ages ranging from 565 to 2626 Ma, indicating that Precambrian continental crust or microcontinents with accretionary arcs are two possible interpretations for the basement underlying the East Junggar terrane. The sampled mafic-intermediate rocks belong to the medium-K to high-K calc-alkaline and shoshonitic series, and the formation of these rocks involved fractional crystallization with little crustal contamination. These Carboniferous mafic-intermediate rocks show depletions in Nb and Ta and enrichments in large ion lithophile elements (e.g. Rb, Ba, U, and Th) and light rare earth elements. The low initial 87Sr/86Sr values (0.7034-0.7042) and positive εNd(t) values (+2.63 to +6.46) of these rocks suggest that they formed from depleted mantle material. The mafic-intermediate rocks were most likely generated by 5-10% partial melting of a mantle source composed primarily of spinel lherzolite withminor garnet lherzolite that had been metasomatized by slab-derived fluids and minor slab melts. In contrast, the felsic rocks in the BS Formation are A-type rhyolites with positive εNd(t) values and young model ages. These rocks are interpreted to be derived from the partial melting of juvenile basaltic lower crustal material. Taken together, the mafic-intermediate rocks formed in a post-collisional extensional setting generated by slap breakoff in the early Carboniferous (342-330 Ma) and the A-type rhyolites formed in a post-collisional extensional setting triggered by the upwelling asthenosphere in the late Carboniferous (330-304 Ma). [ABSTRACT FROM AUTHOR]

Conclusions: It is impossible to make a diagnosis of temporal giant cell granuloma (GCG) before operation because of nonspecific clinical and imaging feature. Surgery is the first-line choice of treatment. Objective: To evaluate the diagnosis and treatment of temporal GCG. Methods: Eight patients with GCG receiving treatment in the Chinese PLA General Hospital between 2001 and 2010 were recruited for the study. These patients' clinical features, imaging and histopathological findings, types of surgery, and results of follow-up evaluations were noted. Results: The group was made up of four males and four females, with a median age of 37 years (range 21-50 years). Four patients had a granuloma on the left side and four on the right of the head. The median duration of the disease was 21 months (range 5-60 months). All patients, except one referred to us for recurring disease, were managed in our hospital. The main symptoms were: hearing loss ( n = 5), tinnitus ( n = 4), otalgia ( n = 3), dizziness ( n = 2), and local masses ( n = 2). Radiological examination of the masses revealed erosion of the temporal bone and base of the skull. There was no definitive diagnosis in any of the patients before surgery. All patients had surgical treatment, six of them by middle cranial fossa approach and two by combined cranio-auricular approach. Surgical complications included partial facial paralysis (three cases) and cerebral edema (one case) but they resolved soon after surgery. One patient was lost to follow-up, but the other seven were followed up over a mean period of 24 months; none has reported a recurrence. [ABSTRACT FROM AUTHOR]

This paper presents a new detailed study including zircon U-Pb-Hf isotopic, whole-rock geochemical and Sr-Nd isotopic analyses of conglomerate and granitic pebbles from the molasse deposits in central Jilin Province, NE China. These data are used to better constrain the Late Permian–Triassic tectonic evolution regarding particularly the final closure of the Paleo-Asian Ocean (PAO) along the Changhun-Yanji suture (CYS). Zircon U-Pb data indicate that the granitic pebbles formed in the end-Permian (254–253 Ma). The youngest detrital zircon age of 231 Ma from the conglomerate, and presence of the overlying Upper Triassic Sihetun Formation suggests that the molasse deposits on the Jin′gui Island formed during the Late Triassic. The end-Permian granitic rocks display high SiO2 (66.07–74.77 wt %), with low MgO (0.55–2.05 wt %) and Mg# (31.61–43.64) values, together with depleted Hf and Nd isotopic values (εHf(t) = +1.61 to +11.62; εNd(t) = +3.3 to +4.2; (87Sr/86Sr)i = 0.706458–0.706842) and juvenile second-stage Hf model ages (1148–512 Ma), suggesting that they were probably generated by the partial melting of a Meso-Neoproterozoic juvenile metabasaltic lower crust. They are characterized by enrichments in large ion lithophile elements (LILEs) and depletions in high field strength elements (HFSEs), with affinities to igneous rocks forming in a subduction-related setting. This, combined with regional coeval subduction-related magmatic rocks, indicates that the PAO still existed along the CYS. In addition, the identification of Late Triassic molasse deposits on the Jin′gui Island in this study, coupled with occurrences of many Early Triassic syn-collisional granitoids along the CYS, indicates that the final closure of the PAO took place prior to the Late Triassic. [ABSTRACT FROM AUTHOR]

The early detection and accurate histopathological diagnosis of gastric cancer increase the chances of successful treatment. The worldwide shortage of pathologists offers a unique opportunity for the use of artificial intelligence assistance systems to alleviate the workload and increase diagnostic accuracy. Here, we report a clinically applicable system developed at the Chinese PLA General Hospital, China, using a deep convolutional neural network trained with 2,123 pixel-level annotated H&E-stained whole slide images. The model achieves a sensitivity near 100% and an average specificity of 80.6% on a real-world test dataset with 3,212 whole slide images digitalized by three scanners. We show that the system could aid pathologists in improving diagnostic accuracy and preventing misdiagnoses. Moreover, we demonstrate that our system performs robustly with 1,582 whole slide images from two other medical centres. Our study suggests the feasibility and benefits of using histopathological artificial intelligence assistance systems in routine practice scenarios. The early detection and accurate histopathological diagnosis of gastric cancer are essential factors that can help increase the chances of successful treatment. Here, the authors report on a digital pathology tool achieving high performance on a real world test dataset and show that the system can aid pathologists in improving diagnostic accuracy. [ABSTRACT FROM AUTHOR]

[Display omitted] • (1) Trachydacites have zircon U-Pb ages of 116 Ma and were coeval with the most extensive thinning of the North China Craton. • (2) 116 Ma trachydacites were related to subduction of Yangtze Craton and Paleo-Pacific plate. • (3) The mantle source underneath the southeastern North China Craton was modified by the subduction of the Yangtze Craton. The North China Craton has witnessed a large-scale lithospheric thinning event in the Mesozoic. The volcanic rocks of the Qingshan Group formed during the destruction of the North China Craton, therefore retaining important information to the deep processes in this region. Zircon U-Pb dating and geochemical analyses have been carried out on volcanic rocks of the Qingshan Group collected from southeastern margin of the Jiaolai Basin. In combination with previously published data on the volcanic rocks of the Qingshan Group, the newly acquired data show that: (1) the volcanic rocks of Qingshan Group in Mashan have SiO 2 content of 62.82 ∼ 66.3 wt% and total alkali content (K 2 O + Na 2 O) of 7.64 ∼ 9.34 wt%. According to TAS diagram, they belong to trachydacite which experienced strong fractionation between LREEs and HREEs with depletion of HFSEs and enrichment of LILEs. (2) The crystallization age of trachydacite in Mashan is 116.4 ± 2.3 Ma (MSWD = 1.9, n = 9). The magma originated from the partial melting of the ancient lower crust and the enriched lithospheric mantle metasomatized by the fluids/melts released from the subducted slab of the Yangtze Craton. (3) The formation age of magmatic rocks in western Shandong is generally earlier than that in eastern Shandong, while the lower crust delamination in western Shandong preceded that in eastern Shandong. The occurrence of magmatism in eastern Shandong is probably attributed to the delamination process in western Shandong as well as the ongoing tectonic activities along the Tan-Lu fault zone. The lithospheric thinning of the North China Craton was mainly controlled by the change of the subduction angle of the Paleo-Pacific plate, and is probably influenced by the continuous activity of the Tan-Lu fault zone in eastern Shandong. [ABSTRACT FROM AUTHOR]

• Provenances of Permo-Triassic clastic rocks in eastern CAOB change from single to bidirectional. • The mixing of the Angaran and Cathaysian floras occurred during the Late Permian-Middle Triassic. • The PAO closed via a scissor-like closure model from west to east with bidirectional subduction. The mixture of the Cathaysian and Angaran floras signifies the evolution of the Paleo-Asian Ocean (PAO). In this study, we present new zircon U-Pb-Hf isotope analyses from the Jiefangcun Formation, Sidonggou Formation, Kedao Group, and Kaishantun Formation in the Yanbian area, NE China. The Kaishantun Formation contains the Kaishantun flora, which is dominated by the Cathaysian flora with a few Angaran elements. The Jiefangcun Formation is characterized by the Jiefangcun flora, which is dominated by the Angaran taxa and contains some Cathaysian elements. The ages of these strata are widely accepted as Permian. However, in this study, the youngest zircon ages of the rock samples from the Jiefangcun Formation, Kedao Group and Sidonggou Formation are 243–245 Ma. Only the Kaishantun Formation is assigned to the Late Permian. The Kaishantun Formation has age peaks at 758, 934, 1856, and 2493 Ma with negative ε Hf (t) values, and this formation is therefore mainly derived from the North China Craton (NCC). In contrast, the Kedao Group and Jiefangcun Formation have zircon age peaks at ~260, 338 and ~460 Ma with positive and negative ε Hf (t) values, at 800–1000 Ma with mostly positive ε Hf (t) values, and at ~1800 Ma with positive and negative ε Hf (t) values, which suggest bidirectional provenances, including the Jiamusi-Mongolia Block and the NCC. Thus, the mixing between the Angaran and Cathaysian floras occurred during the Late Permian-Middle Triassic, suggesting a scissor-like closure model of the PAO, and the Solonker suture zone extends eastward to the Yanbian area with bidirectional subduction of the PAO. [ABSTRACT FROM AUTHOR]

Introduction: Lymph node metastasis is one of the most common ways of tumour metastasis. The presence or absence of lymph node involvement influences the cancer's stage, therapy, and prognosis. The integration of artificial intelligence systems in the histopathological diagnosis of lymph nodes after surgery is urgent., Methods: Here, we propose a pan-origin lymph node cancer metastasis detection system. The system is trained by over 700 whole-slide images (WSIs) and is composed of two deep learning models to locate the lymph nodes and detect cancers., Results: It achieved an area under the receiver operating characteristic curve (AUC) of 0.958, with a 95.2% sensitivity and 72.2% specificity, on 1,402 WSIs from 49 organs at the National Cancer Center, China. Moreover, we demonstrated that the system could perform robustly with 1,051 WSIs from 52 organs from another medical centre, with an AUC of 0.925., Conclusion: Our research represents a step forward in a pan-origin lymph node metastasis detection system, providing accurate pathological guidance by reducing the probability of missed diagnosis in routine clinical practice., (© 2024 S. Karger AG, Basel.)

Purpose: To investigate the sensitivity of SARS-CoV-2 testing in specimens collected from the anterior nasal vestibules of COVID-19 patients., Methods: A cross-sectional analysis was performed on 30 patients with a confirmed diagnosis of COVID-19 at the Shanghai Public Health Clinical Center from March 14, 2020 to March 21, 2020. Paired specimens were collected from both the anterior nasal vestibule and the oropharynx from all patients. All specimens were tested for SARS-CoV-2 using reverse transcription-polymerase chain reaction (RT-PCR) assays., Results: Of the 30 patients with confirmed COVID-19, 17 patients (56.7%) tested positive for SARS-CoV-2 when oropharyngeal specimens were used, while 20 patients (66.7%) tested positive when nasal swab specimens were used. There was no statistically significant difference in sensitivity between the two methods., Conclusions: Respiratory swabs collected from the nasal vestibule offer a less invasive alternative to oropharyngeal swabs for specimen collection in the detection of SARS-CoV-2 infection, and have adequate sensitivity., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Li, Wei, Yang, He, Shen, Qi, Han, Song, Zhu, Ma, Lin, Yuan, Zhao, Lu and Zhou.)

Background: Novel coronavirus pneumonia (COVID-19) is prevalent around the world. We aimed to describe epidemiological features and clinical course in Shanghai. Methods: We retrospectively analysed 325 cases admitted at Shanghai Public Health Clinical Center, between January 20 and February 29, 2020. Results: 47.4% (154/325) had visited Wuhan within 2 weeks of illness onset. 57.2% occurred in 67 clusters; 40% were situated within 53 family clusters. 83.7% developed fever during the disease course. Median times from onset to first medical care, hospitalization and negative detection of nucleic acid by nasopharyngeal swab were 1, 4 and 8 days. Patients with mild disease using glucocorticoid tended to have longer viral shedding in blood and feces. At admission, 69.8% presented with lymphopenia and 38.8% had elevated D-dimers. Pneumonia was identified in 97.5% (314/322) of cases by chest CT scan. Severe-critical patients were 8% with a median time from onset to critical disease of 10.5 days. Half required oxygen therapy and 7.1% high-flow nasal oxygen. The case fatality rate was 0.92% with median time from onset to death of 16 days. Conclusion: COVID-19 cases in Shanghai were imported. Rapid identification, and effective control measures helped to contain the outbreak and prevent community transmission.

Background: Enteroviruses cause hand, foot and mouth disease (HFMD). The host B-cells recognize the viral proteins and provoke humoral responses. Deciphering the B-cell responses to the viral epitopes helps diagnosis and vaccine development., Objectives: The objective of the present study was to investigate for the first time the landscape of genome-wide linear B-cell epitopes of enterovirus 71 in HFMD population., Study Design: The peptides encompassing the entire coding region of EV71 were chemically synthesized and displayed on a microarray. The peptide microarray was used to screen serum samples from an HFMD population, including EV71-, CAV10-, CAV16- and CAV6-infected patients. We identified the dominant epitope-containing-peptides (DECPs) that react with the sera of more than 20% of the HFMD population and the common DECPs that cross-react with the sera from other enteroviruses-infected population., Results: Ten DECPs reacting with IgM and 9 DECPs reacting with IgG antibodies were identified, of which, 6 IgM and 5 IgG common DECPs cross-reacted with the sera from other enteroviruses. Some DECPs preferentially reacted with IgG or IgM antibodies and some epitope-antibody interactions correlated with the severity of HFMD., Conclusions: We uncovered the DECPs and the common DECPs among a group of enteroviruses in HFMD population and found that some epitope-antibody reactions were associated with the outcome of HFMD. These data may guide developing vaccines against the enteroviruses and help the diagnosis and prognosis of HFMD., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Background: The clinical features of avian-origin influenza virus A (H7N9) virus infection have been extensively characterized, but viral RNA detection in extra-pulmonary samples has seldom been studied., Objectives: To study shedding of viral RNA in extra pulmonary samples in patients with avian influenza H7N9 infections., Study Design: A retrospective study of throat swabs, urine, fecal samples and sera collected sequentially from 18 hospitalized patients with H7N9 infections in Shanghai, China, between April and July in 2013 was conducted., Results: Viral RNA could be detected in urine samples from 17 patients, in fecal samples from 15 and in sera from 14 with a real-time reverse transcription polymerase chain reaction. The median duration of shedding of viral RNA was 19.7 days in throat swabs, 22 days in feces, 21.1 days in urines and 16.2 days in sera, indicating prolonged shedding of viral RNA in feces and urine compared with that in throat swabs. Prolonged duration of viral RNA detection in throat swabs and urine samples was observed in more severe patients. Moreover, in previously reported oseltamivir resistant patients, the NA gene with a 292K mutation could also be detected in their extra-pulmonary as well as in their respiratory samples., Conclusion: Our data indicated a high frequency of viral RNA detection in feces, urine and sera in H7N9-infected patients and pointed out the potential risk of transmission., (Copyright © 2015 Elsevier B.V. All rights reserved.)

To investigate the diversity of viral flora, we used metagenomics to study the viral communities in a pooled faecal sample of 27 diarrhoeic piglets from intensive commercial farms in China. The 15 distinct mammalian viruses identified in the pooled diarrhoeic sample were, in order of abundance of nucleic acid sequence, Porcine epidemic diarrhea virus (PEDV), sapovirus, porcine bocavirus-4 (PBoV-4), sapelovirus, torovirus, coronavirus, PBoV-2, stool-associated single-stranded DNA virus (poSCV), astrovirus (AstV), kobuvirus, posavirus-1, porcine enterovirus-9 (PEV-9), porcine circovirus-like (po-circo-like) virus, picobirnavirus (PBV) and Torque teno sus virus 2 (TTSuV-2). The prevalence rate of each virus was verified from diarrhoeic and healthy piglets by PCR assay. A mean of 5.5 different viruses were shed in diarrhoeic piglets, and one piglet was in fact co-infected with 11 different viruses. By contrast, healthy piglets shed a mean of 3.2 different viruses. Compared with samples from healthy piglets, the co-infection of PEDV and PBoV had a high prevalence rate in diarrhoea samples, suggesting a correlation with the appearance of diarrhoea in piglets. Furthermore, we report here for the first time the presence of several recently described viruses in China, and the identification of novel genotypes. Therefore, our investigation results provide an unbiased survey of viral communities and prevalence in faecal samples of piglets., (© 2014 The Authors.)

Background: On March 30, a novel influenza A subtype H7N9 virus (A/H7N9) was detected in patients with severe respiratory disease in eastern China. Virological factors associated with a poor clinical outcome for this virus remain unclear. We quantified the viral load and analysed antiviral resistance mutations in specimens from patients with A/H7N9., Methods: We studied 14 patients with A/H7N9 disease admitted to the Shanghai Public Health Clinical Centre (SPHCC), China, between April 4, and April 20, 2013, who were given antiviral treatment (oseltamivir or peramivir) for less than 2 days before admission. We investigated the viral load in throat, stool, serum, and urine specimens obtained sequentially from these patients. We also sequenced viral RNA from these specimens to study the mutations associated with resistance to neuraminidase inhibitors and their association with disease outcome., Findings: All patients developed pneumonia, seven of them required mechanical ventilation, and three of them further deteriorated to become dependent on extracorporeal membrane oxygenation (ECMO), two of whom died. Antiviral treatment was associated with a reduction of viral load in throat swab specimens in 11 surviving patients. Three patients with persistently high viral load in the throat in spite of antiviral therapy became ECMO dependent. An Arg292Lys mutation in the virus neuraminidase (NA) gene known to confer resistance to both zanamivir and oseltamivir was identified in two of these patients, both also received corticosteroid treatment. In one of them, wild-type sequence Arg292 was noted 2 days after start of antiviral treatment, and the resistant mutant Lys292 dominated 9 days after start of treatment., Interpretation: Reduction of viral load following antiviral treatment correlated with improved outcome. Emergence of NA Arg292Lys mutation in two patients who also received corticosteroid treatment led to treatment failure and a poor clinical outcome. The emergence of antiviral resistance in A/H7N9 viruses, especially in patients receiving corticosteroid therapy, is concerning, needs to be closely monitored, and considered in pandemic preparedness planning., Funding: National Megaprojects of China for Infectious Diseases, Shanghai Municipal Health and Family Planning Commission, the National Key Basic Research Program of China, Ministry of Science and Technology, and National Natural Science Foundation of China., (Copyright © 2013 Elsevier Ltd. All rights reserved.)