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Microorganisms change the properties of microplastics, at the same time, microplastics can also affect the distribution of microorganisms. To investigate this issue, we chose to study Jianhu Lake, a plateau lake in southwestern China, by collecting data at three sampling locations. The microplastics and bacterial communities in the sediment columns of Jianhu Lake were sampled within a 0 to 60 cm profile, and the basic characteristics of microplastic abundance, shape, color, size, and polymer type were determined accordingly, via their collection, separation, extraction, and identification. The bacterial community in the sediment samples were identified using 16S rRNA high-throughput sequencing, and we assessed whether those microplastic characteristics influenced the community composition and structure. We found the abundance of microplastics ranged from 624 to 3050 particles/kg (dw [dry weight]) in the three sediment columns. Line microplastics accounted for the largest proportion and these were found distributed in each layer. The polymer types present in the largest proportions were rayon (RY), polyester terephthalate (PET) and low-density styrene-butadiene-styrene (SBS). Among the bacterial communities in the sediment columns, the dominant phyla were Chloroflexi, Sva0485, Acidobacteriota, etc. The co-occurrence network analysis between the bacterial community and microplastic features in the sediment columns of Jianhu Lake revealed that there was a correlation between them, and the network were more complex at a depth of 20-40 cm. Our results demonstrate that microplastics can affect the diversity and structural characteristics of microbial communities in a lake ecosystem., Competing Interests: Declarations. Competing interests: The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)

The South China Sea (SCS) is a marginal sea characterized by strong land-sea biogeochemical interactions. SCS has a distinctive landscape with a multitude of seamounts in its basin. Seamounts create "seamount effects" that influence the diversity and distribution of planktonic microorganisms in the surrounding oligotrophic waters. Although the vertical distribution and community structure of marine microorganisms have been explored in certain regions of the global ocean, there is a lack of comprehensive microbial genomic surveys for uncultured microorganisms in SCS, particularly in the seamount regions. Here, we employed a metagenomic approach to study the uncultured microbial communities sampled from the Xianbei seamount region to the North Coast waters of SCS. A total of 1887 non-redundant prokaryotic metagenome-assembled genomes (MAGs) were reconstructed, of which, 153 MAGs were classified as high-quality MAGs based on the MIMAG standards. The community structure and genomic information provided by this dataset could be used to analyze microbial distribution and metabolism in the SCS., (© 2024. The Author(s).)

Yellow-feathered chickens have great nutritional value and are widely and traditionally used in China, on an industrial scale as broilers. The presence of intestinal microbes has been shown to correlate with poultry performance and serves as an essential reservoir of antibiotic resistance genes (ARGs). Antibiotic resistance is a major public health concern. Here, we investigated functional characteristics of the gut microbiome of indigenous Chinese yellow-feathered chickens (the Huiyang Bearded, Xinghua, Huaixiang, Zhongshan Shanlan, Qingyuan Partridge, and Yangshan chickens) through metagenomic sequencing and reconstructed 409 draft genomes, including 60 novel species and 6 novel genera. Furthermore, we assessed the functions of the intestinal microbial communities and examined the ARGs within them. The results showed that the microbial populations of yellow-feathered broilers were primarily dominated by Bacteroidetes and Firmicutes at the phylum level and Bacteroides at the genus level. Furthermore, the Qingyuan Partridge chicken showed a significantly higher abundance of Prevotella than the other five breeds of chicken. Principal coordinates analysis indicated significant differences in the structures of microbial communities and ARGs, based on the binary Jaccard distance, among the six chicken breeds. Moreover, 989 ARGs conferring tetracycline, multidrug, and aminoglycoside resistance were identified, which represented more than 80% of the faecal resistomes; the most abundant gene in the yellow-feathered chickens was tet(Q). In addition, we found the greatest abundance of resistance genes in Xinghua chickens, indicating that Xinghua chickens are highly resistant to antibiotics. Overall, our findings revealed differences in the gut microbial community structure of indigenous Chinese yellow-feathered broiler breeds and the composition and characteristics of ARGs and antibiotic resistance that enabled us to reconstruct the yellow-feathered chicken gut microbial community genomes. The current data significantly improves our knowledge of the gut microbiome and antibiotic resistance of popular broiler breeds in China. [ABSTRACT FROM AUTHOR]

Dissolved organic matter (DOM) provides carbon substrates and energy sources for sediment microbes driving benthic biogeochemical processes. The interactions between microbes and DOM are dynamic and complex and require the understanding based on fine-scale microbial community and physicochemical profiling. In this study, we characterized the porewater DOM composition in a 300-cm sediment core from the Pearl River estuary, China, and examined the interactions between DOM and archaeal and bacterial communities. DOM composition were highly stratified and associated with changing microbial communities. Compared to bacteria, the amplicon sequence variants of archaea showed significant Pearson correlations (r ≥ 0.65, P < 0.01) with DOM molecules of low H/C ratios, high C number and double bond equivalents, indicating that the distribution of archaea was closely correlated to recalcitrant DOM while bacteria were associated with relatively labile compounds. This was supported by the presence of auxiliary enzyme families essential for lignin degradation and bcrABCD, UbiX genes for anaerobic aromatic reduction in metagenome-assembled genomes of Bathyarchaeia. Our study demonstrates that niche differentiation between benthic bacteria and archaea may have important consequences in carbon metabolism, particularly for the transformation of recalcitrant organic carbon that may be predominant in aged marine sediments. [ABSTRACT FROM AUTHOR]

A soil-borne fungal parasite (Plasmodiophora brassicae Woron) has regularly caused 50-60% reductions in the seasonal yield of a 35 years old cabbage plantation under garlic revaccination in China. We investigated the impact of soil conditioners on soil chemical properties and bacterial communities in order to recommend actions to address increasing frequencies of root-resistant cabbage infections. The results showed urease and polyphenol oxidase activities increased by 10.4-15.8% in the topsoil after two-years of quicklime and organic fertilizer application. High throughput sequencing data showed significant increases in the relative abundance of bacteria with potential for biocontrol (Xanthomonadales, Pseudomonas, and Bacillus) under lime and bioorganic fertilizer. Also, according to a forward-selection CANOCO analysis, in this case the two most important variables contributing to the variation in bacterial communities’ composition were soil organic matter and total nitrogen. [ABSTRACT FROM AUTHOR]