1. Sceptridium ternatum extract exerts antiasthmatic effects by regulating Th1/Th2 balance and the expression levels of leukotriene receptors in a mouse asthma model.
- Author
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Yuan Y, Yang B, Ye Z, Zhang M, Yang X, Xin C, Lin M, and Huang P
- Subjects
- Animals, Anti-Asthmatic Agents isolation & purification, Asthma immunology, Asthma pathology, China, Disease Models, Animal, Drugs, Chinese Herbal isolation & purification, Female, Lung drug effects, Lung pathology, Mice, Mice, Inbred BALB C, Ovalbumin immunology, Receptors, Leukotriene immunology, Anti-Asthmatic Agents therapeutic use, Asthma drug therapy, Drugs, Chinese Herbal therapeutic use, Plants, Medicinal chemistry, Receptors, Leukotriene genetics, Th1-Th2 Balance drug effects
- Abstract
Aim of the Study: Sceptridium ternatum Lyon (ST), a common Chinese herb, has been used in treatment of allergic asthma and whooping cough. In the present study, we investigated the Th1/Th2 ratio of peripheral blood and mRNA levels of leukotriene receptors after the treatment of ST in allergic asthma mouse model., Materials and Methods: Mouse asthma model was developed by ovalbumin (OVA) sensitization followed by the inhalation of aerosol allergen. Montelukast (10mg/kg), as a positive control drug, and ST were administrated six days before the OVA sensitization for ten days. Airway responsiveness was evaluated by the Medlab 12.0 biological signal processing system. The ratio of Th1/Th2 cells was determined by flow cytometry. The expression level of Cyslt1 was measured by PCR. Pathological changes of lung tissues were examined by H&E staining., Results: ST significantly reduced the airway responsiveness, elevated the ratio of Th1/Th2, and decreased Cyslt1 mRNA level in a dose-dependent manner. High-dose ST distinctly prevented the pathological changes of lung tissues., Conclusion: High-dose ST had the same efficacy as Montelukast in a mouse asthma model, and ST could be a potential anti-asthmatic agent., (© 2013 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2013
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