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In this research, a nanoscale DNA structure of analogic China map is created. The nanostructure of roughly 150 nm in diameter with a spatial resolution of 6 nm is purely constructed by folding DNA. The picture observed by atomic force microscopy (AFM) is almost identical with the designed shape. The DNA origami technology invented by Rothemund in 2006 is employed in the construction of this shape, which has proved the capability of constructing almost any complicated shape enabled by DNA origami, and provides new bottom-up method for constructing nanostructures. [ABSTRACT FROM AUTHOR]

A novel actinobacterium, designated strain NEAU-7082 T , was isolated from rhizosphere soil of wheat ( Triticum aestivum L.) and characterized by using a polyphasic approach. Morphological and chemotaxonomic characteristics confirmed the affiliation of strain NEAU-7082 T to the genus Glycomyces . 16S rRNA gene sequence analysis indicated that strain NEAU-7082 T belonged to the genus Glycomyces and was closely related to Glycomyces mayteni JCM 16217 T (99.0 % 16S rRNA gene sequence similarity), Glycomyces sambucus DSM 45047 T (98.4 %), Glycomyces scopariae DSM 44968 T (98.3 %), Glycomyces paridis DSM 102295 T (98.1 %), Glycomyces artemisiae NBRC 109773 T (98.0 %) and Glycomyces dulcitolivorans DSM 105121T (97.9 %). Phylogenetic analysis using the 16S rRNA gene sequence showed that the strain formed a stable clade with G. mayteni JCM 16217 T and clustered with G. sambucus DSM 45047 T , G. scopariae DSM 44968 T , G. artemisiae NBRC 109773 T and G. dulcitolivorans DSM 105121T in the genus Glycomyces . The cell wall contained meso -diaminopimelic acid and the whole-cell hydrolysates were glucose and xylose. The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), glycolipid (GL), phosphatidylinositol mannoside (PIM) and an unidentified lipid (UL). The menaquinones were MK-11(H 4 ), MK-11 and MK-10. Major fatty acids were anteiso-C 15 : 0 , iso-C 16 : 0 and anteiso-C 17 : 0 . These chemotaxonomic data substantiated the affiliation of strain NEAU-7082 T to the genus Glycomyces . The DNA G+C content was 71.3 mol%. A combination of DNA-DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-7082 T could be distinguished from its closest relatives. Therefore, strain NEAU-7082 T is considered to represent a novel species of the genus Glycomyces , for which the name Glycomyces albidus sp. nov. is proposed. The type strain is NEAU-7082 T (=CCTCC AA 2019045 T =JCM 33458 T ).

A novel actinobacterium, designated strain NEAU-THZ27 T , was isolated from soil collected from the Cornel peak in Jiaozuo, Henan Province, PR China and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics of the strain coincided with those of members of the genus Kribbella . The results of 16S rRNA gene sequence analysis showed that strain NEAU-THZ27 T belongs to the genus Kribbella and was most closely related to Kribbella podocarpi YPL1 T (98.96 %), Kribbella karoonensis Q41 T (98.89 %), Kribbella aluminosa HKI 0478 T (98.86%) and Kribbella hippodromi S1.4 T (98.85 %), similarities to other type strains of species of the genus Kribbella were found to be less than 98.7 %. Phylogenetic analyses using the 16S rRNA gene sequence and multilocus sequence analysis using the concatenated gene sequences of the gyrB , rpoB , recA , relA and atpD genes all showed that the strain formed a separate branch in the genus Kribbella . The cell wall contained ll-diaminopimelic acid as the major diamino acid and the whole-cell hydrolysates were ribose and glucose. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinone was MK-9(H 4 ). Major fatty acids were iso-C 16 : 0 , iso-C 14 : 0 and anteiso-C 15 : 0 , these chemotaxonomic data supported the affiliation of strain NEAU-THZ27 T to the genus Kribbella . The DNA G+C content was 68.0 mol%. Furthermore, the strain could be clearly distinguished by concatenated gene genetic distances, the combination of DNA-DNA hybridization results and some phenotypic characteristics. Therefore, it is proposed that strain NEAU-THZ27 T represents a novel species of the genus Kribbella , for which the name Kribbella jiaozuonensis sp. nov. is proposed. The type strain is NEAU-THZ27 T (=CGMCC 4.7504 T =DSM 105535 T ).

Three Gram-stain positive, aerobic actinomycete strains, designated NEAU-A15 T , NEAU-A13 and NEAU-C4, were isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Langfang, Hebei Province, China. Based on their morphological characteristics, biochemical features and molecular phylogenetic studies, these strains were concluded to belong to a new member of the genus Glycomyces. 16S rRNA gene sequence analysis showed that strain NEAU-A15 T shares 99.91% and 99.80% 16S rRNA gene sequence similarity with NEAU-A13 and NEAU-C4, respectively, and these three strains showed high sequence similarities to Glycomyces algeriensis DSM 44727 T (99.24, 99.45, 99.38%), Glycomyces lechevalierae DSM 44724 T (98.97, 99.17, 99.11%) and Glycomyces rutgersensis DSM 43812 T (98.83, 99.04, 98.97%). Phylogenetic analysis indicated that these three strains clustered together and formed a cluster with Glycomyces tritici NEAU-C2 T (97.30, 97.73, 99.48%), G. algeriensis DSM 44727 T , G. lechevalierae DSM 44724 T and G. rutgersensis DSM 43812 T . These three strains were observed to contain MK-10(H 2 ), MK-10(H 6 ) and MK-11 as menaquinones. The whole cell sugar profiles were found to contain galactose, ribose and xylose. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylinositol, phosphatidylglycerol, phosphoglycolipid, phosphatidylinositol mannoside and an unidentified glycolipid. The major fatty acids were identified as anteiso-C 15:0 , iso-C 16:0 , anteiso-C 17:0 and iso-C 15:0 . The DNA-DNA hybridization values between strain NEAU-A15 T and NEAU-A13/NEAU-C4 were 86.2 ± 2.3% and 83.4 ± 3.5%, respectively. The values between these three strains and their close phylogenetic relatives were 48-52%, supporting the conclusion that they belong to a distinct genomic species. An array of phenotypic characteristics also differentiated these isolates from their closely related species. On the basis of the genetic and phenotypic properties, strains NEAU-A15 T , NEAU-A13 and NEAU-C4 can be classified as representatives of a novel species of the genus Glycomyces, for which the name Glycomyces luteolus sp. nov., is proposed. The type strain is NEAU-A15 T (= DSM 104643 T  = CGMCC 4.7394 T ).

Brain-derived neurotrophic factor (BDNF) is the most widely distributed neurotrophin in the central nervous system (CNS), and performs many biological functions such as neural survival, differentiation, and plasticity. Previous studies have suggested that variants in the BDNF gene increase the risk of schizophrenia. In this study, we genotyped one (GT)n dinucleotide repeat and three SNPs (rs6265, rs2030324, and rs2883187) in a Chinese sample (617 cases and 672 controls). In addition, we performed an updated meta-analysis based on 16 population-based case-control studies examining association between rs6265 and schizophrenia. In single-locus analysis, no significant association was found between BDNF polymorphisms and schizophrenia in our subjects. The meta-analysis based on Asian and Caucasian subjects did not give positive result that rs6265 is associated with schizophrenia. However, haplotype analysis found a common four-locus haplotype is protective against schizophrenia (Case 3.1% vs Control 7%, p=0.0011). Our data provides evidence that BDNF is a susceptibility gene for schizophrenia in Chinese subjects.