1. Molecular background of Rh D-positive, D-negative, Del and weak D phenotypes in Chinese.
- Author
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Shao, C.-P., Maas, J.-H., Su, Y.-Q., Köhler, M., and Legler, T. J.
- Subjects
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PHENOTYPES , *HUMAN genetics - Abstract
Background and Objectives The aim of this study was to elucidate the genetic background of D-negative and Del in the Chinese population. Materials and Methods We investigated nine D-positive, 76 D-negative, 26 Del and three weak D Chinese individuals by amplification and sequencing of the complete coding region of the RHD gene from genomic DNA. A new RHD polymerase chain reaction with sequence-specific primers (PCR–SSP) method was developed with optimized specificity for typing Chinese individuals. Results In D-positive samples the RHD sequence was in complete concordance with RHD in other populations. In 12 of 76 (15·8%) D-negative individuals we detected regions of RHD. Three new alleles were found. All 26 Del , as well as two weak D, individuals carried an RHD 1227A allele. In the remaining weak D sample we identified a weak D type 15. Conclusions It should now be possible to correctly predict the RhD phenotype in Chinese subjects. Del can also be designated as a particular weak D type. [ABSTRACT FROM AUTHOR]
- Published
- 2002
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