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Using data from 56 tight gas wells from the study field (Y field) in the Ordos basin of China, this paper presents performance-based reservoir characterization of the study field from production data and geophysical data. Post-fracturing evaluation is realized by applying our new modified production decline type curves for fractured wells. Compared to traditional type curves, our newly proposed modified dimensionless type curves help identify field data diagnostics for various flow regimes of fractured wells and also facilitate the curve matching process with real data to obtain fruitful and crucial reservoir and fractured well information, including key parameters such as reservoir flowing capacity (kh), well productivity, fracture length, drainage area and original gas in place. This paper intends to promote the extensive application of this new technique. With the support of the reservoir information provided by production decline analysis using our modified type curves, the commercial flow units are delineated in terms of interrelated porosity-permeability of sandstone based on pore throat aperture crossplotting and corresponding flow unit productivity. Furthermore, two crossplots of well logging interpreted porosity versus resistivity are constructed, suggesting their good correlated relationships with relative flow unit productivity and initial gas abundance in place, respectively. The two crossplots enable qualitative evaluation of formation penetrated by well, which makes them very useful and practical as wireline logging is basically available for every well. The well production routine is also analyzed systematically by considering a well's inflow performance, tubing performance and minimal liquid-carrying gas flow rate to investigate if a gas well is producing at optimal conditions or if a measure should be taken to improve the well's production. Through analysis of the Y field, this study introduces an integrated workflow with the support of the new modified type curves to effectively help understand the reservoir characteristics and the flow behaviors of the tight gas field. The key takeaway from this study is that the new modified dimensionless production decline curves in terms of q D M   v s.   t D M can be applied in field practice to achieve a systematically comparable understanding of the performance of MHFHWs globally. [ABSTRACT FROM AUTHOR]

Background. Vitamin D deficiency can lead to the increased severity and prevalence of metabolic disorders. However, the relationship between levels of 25-hydroxyvitamin D (25(OH)D) and peripheral arterial disease (PAD) is controversial. Therefore, the purpose of our study was to explore the relationship between 25(OH)D levels and PAD in middle-aged and elderly type 2 diabetes mellitus (T2DM) patients in China. Methods. In this study, a total of 183 patients with T2DM were enrolled and categorized into groups with or without PAD. Clinical and biochemical parameters were assessed, and a Pearson analysis was used to identify a possible association between levels of 25(OH)D and glycated hemoglobin (HbA1c). Some biochemical parameters were also assessed in the T2DM patients with PAD according to vitamin D status. Interactions were also explored among HbA1c control, 25(OH)D levels, and PAD. The possible risk factors for PAD were measured by multivariable logistic regression analyses. Results. Firstly, the parameters including age, HbA1c, and disease duration between T2DM and T2DM+PAD groups showed significantly different. In addition, the frequency of smoking in the group of T2DM patients was significantly less than that in the T2DM patients with the PAD group, while the frequency of well-controlled HbA1c in the patients with T2DM was significantly higher. There is a trend that the levels of 25(OH)D and HbA1c are correlated, but no interactions among vitamin D deficiency, HbA1c control, and PAD were found. However, HbA1c significantly differed between groups with vitamin D deficiency and insufficiency in the T2DM patients with PAD. According to the multivariate logistic regression analyses, the PAD risk factors of T2DM patients were family history of diabetes, smoking, age, disease duration, HbA1c, and LDL. Conclusions. The findings demonstrate that the deficiency of vitamin D level is not related to PAD, but HbA1c may be linked to the presence of PAD in middle-aged and elderly patients with T2DM in China. [ABSTRACT FROM AUTHOR]

Acetyl ligation to the amino acids in a protein is an important posttranslational modification. However, in contrast to lysine acetylation, N-terminal acetylation is elusive in terms of its cellular functions. Here, we identify Nat3 as an N-terminal acetyltransferase essential for autophagy, a catabolic pathway for bulk transport and degradation of cytoplasmic components. We identify the actin cytoskeleton constituent Act1 and dynamin-like GTPase Vps1 (vacuolar protein sorting 1) as substrates for Nat3-mediated N-terminal acetylation of the first methionine. Acetylated Act1 forms actin filaments and therefore promotes the transport of Atg9 vesicles for autophagosome formation; acetylated Vps1 recruits and facilitates bundling of the SNARE (soluble N-ethylmaleimide-sensitive factor activating protein receptor) complex for autophagosome fusion with vacuoles. Abolishment of the N-terminal acetylation of Act1 and Vps1 is associated with blockage of upstream and downstream steps of the autophagy process. Therefore, our work shows that protein N-terminal acetylation plays a critical role in controlling autophagy by fine-tuning multiple steps in the process., Competing Interests: Declaration of interests All authors declare no competing interests., (Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.)