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The mpox outbreak since 2022 had attacked the community of men who have sex with men (MSM) heavily. This large cross‐sectional study investigated the levels and associated factors of mpox risk perception among young MSM (YMSM) aged 18–29 years in six provincial regions of China in September 2022. The participants were recruited via facility‐based sampling. Mpox risk perception was measured by perceived susceptibility and perceived severity of mpox. Geodetector was used to measure stratified heterogeneity of mpox risk perception. Univariate and multivariable linear regressions were used to examine the factors associated with mpox risk perception. A total of 2493 participants were included with a mean age of 24.6 years. The proportion of perceiving a susceptibility of mpox under different scenarios ranged 3.7%–17.0% and that of perceiving a severity of mpox ranged 81.6%–83.2%. Stratified heterogeneity of perceived susceptibility, perceived severity, and overall perceived risk of mpox were observed in several characteristics such as study sites, monthly income, risk behaviors, and psychosocial factors. Multivariable regression showed the level of mpox risk perception was positively associated with having in‐person gathering activities (ba = 0.457, 95% CI: 0.208, 0.705), history of HIV infection (ba = 0.431, 95% CI: 0.028, 0.834), depressive symptoms (ba = 0.069, 95% CI: 0.049, 0.090), and self‐stigma to MSM identity (ba = 0.047, 95% CI: 0.024, 0.071). The Chinese YMSM showed a high level of perceived severity of mpox but a low level of perceived susceptibility. It is warranted to strengthen targeted risk communication of mpox, develop comprehensive and unstigmatized health messages, and provide mental health support for YMSM. [ABSTRACT FROM AUTHOR]

This paper studies the influence of large-size cave on pressure transient characteristics in fracture-caved carbonate gas reservoirs (FCCGR). With the rapid increase of energy demand, the exploration and development of unconventional oil and gas becomes more and more important. In recent years, many FCCGR have been discovered in western China and contribute significantly to Chinese gas reserves. However, with the presence of large-size cave, FCCGR have complex pore structures and strong heterogeneity. Traditional pressure transient analysis models cannot describe the gas flow accurately. This paper develops a novel pressure transient analysis model for FCCGR by coupling the fluctuating pressure and minor energy loss. Based on the solutions, the typical curves are plotted to analyze the pressure transient characteristics. It is found that the flow process can be subdivided into six stages, including the following: (I) wellbore storage, (II) first transition stage, (III) cave storage, (IV) second transition stage, (V) interporosity flow, and (VI) radial flow. The findings indicate that a concave is added, and the wellbore storage occurs earlier due to the existence of cave. Then, the influences of key parameters are studied. The pressure propagation coefficient and cave volume factor influence the stages I, II, III, and IV. When pressure propagation coefficient increases, the wellbore storage becomes larger and cave storage becomes smaller. The first concave moves to upper right. When cave volume factor increases, the wellbore storage occurs earlier and the curves move left in stage I. Interporosity flow factor and storage ratio influence the location and depth of the second concave. Finally, a field gas well is interpreted by using the proposed model, which verifies the reliability and correctness of the model. The findings of this study can help to better understand the influence of large-size cave on pressure transient characteristics. In addition, it can help engineers invert the cave volume, which is of great significance for the development in FCCGR. [ABSTRACT FROM AUTHOR]

Viral seed transmission causes the spread of many plant viral diseases. Pyrusbetulifolia and P. calleryana are important rootstock germplasms for pear production in China. This study revealed the widespread infection of apple stem grooving virus (ASGV), apple chlorotic leaf spot virus (ACLSV), and apple stem pitting virus (ASPV) in maternal trees of P. betulifolia and P. calleryana by nested multiplex reverse transcription-polymerase chain reaction (nmRT-PCR) assays. Seeds from eight P. betulifolia and two P. calleryana trees had positive rates of 15.9–73.9%, 0–21.2%, and 40.4% for ASGV, ASPV, and ACLSV, respectively. At the cotyledon and 6–8 true leaf stages, seedlings grown from seeds of infected trees gave positive rates of 5.4% and 9.3% for ASGV, 6.7% and 15.6% for ACLSV, and 0% and 2.7% for ASPV, respectively. Incidence in nursery P. betulifolia seedlings of 10.1%, 5.3%, and 3.5% were determined for ASGV, ACLSV, and ASPV, respectively. The nucleotide sequences of coat protein (CP) and movement protein coding genes of both ASGV and ASPV, and CP gene of ACLSV from maternal trees, seeds, and seedlings were analyzed. Sequence identities and phylogenetic comparison with corresponding sequences from GenBank demonstrated that molecular variation occurred within ASGV, ACLSV, and ASPV isolates, with most sequences determined here had close relationships with reported isolates infecting pear or formed independent clades. This is the first report on the seed transmission and the molecular characteristics of these viruses infecting two rootstock species. These findings provided important evidence in management effort for pear viral diseases. [ABSTRACT FROM AUTHOR]

Apple rubbery wood virus 2 (ARWV-2) and citrus virus A (CiVA) belong to a recently approved family Phenuiviridae in the order Bunyavirales and possess negative-sense single-stranded RNA genomes. In this study, the genome sequence of three ARWV-2 isolates (S17E2, LYC2, and LYXS) and a CiVA isolate (CiVA-P) infecting pear trees grown in China were characterized using high-throughput sequencing combined with conventional reverse-transcription PCR (RT-PCR) assays. The genome-wide nt sequence identities were above 93.6% among the ARWV-2 isolates and above 93% among CiVA isolates. Sequence comparisons showed that sequence diversity occurred in the 5′ untranslated region of the ARWV-2 genome and the intergenic region of the CiVA genome. For the first time, this study revealed that ARWV-2 proteins Ma and Mb displayed a plasmodesma subcellular localization, and the MP of CiVA locates in cell periphery and can interact with the viral NP in bimolecular fluorescence complementation assays. RT-PCR tests disclosed that ARWV-2 widely occurs, while CiVA has a low incidence in pear trees grown in China. This study presents the first complete genome sequences and incidences of ARWV-2 and CiVA from pear trees and the obtained results extend our knowledge of the viral pathogens of pear grown in China. [ABSTRACT FROM AUTHOR]

Background: Mammography-based breast cancer screening has been widely implemented in many developed countries. Evidence was needed on participation and diagnostic performance of population-based breast cancer screening using ultrasound in China. Methods: We used data from the Cancer Screening Program in Urban China in Beijing from 2014 to 2019 and was followed up until July 2020 by matching with the Beijing Cancer Registry database. Eligible women between the ages of 45 and 69 years were recruited from six districts and assessed their risk of breast cancer through an established risk scoring system. Women evaluated to be at high risk of breast cancer were invited to undergo both ultrasound and mammography. Participation rates were calculated, and their associated factors were explored. In addition, the performance of five different breast cancer screening modalities was evaluated in this study. Results: A total of 49,161 eligible women were recruited in this study. Among them, 15,550 women were assessed as high risk for breast cancer, and 7,500 women underwent ultrasound and/or mammography as recommended, with a participation rate of 48.2%. The sensitivity of mammography alone, ultrasound alone, combined of ultrasound and mammography, ultrasound for primary screening followed by mammography for triage, and mammography for preliminary screening followed by ultrasound for triage were19.2%, 38.5%, 50.0%, 46.2%, and 19.2%, and the specificity were 96.1%, 98.6%, 94.7%, 97.6%, 95.7%, respectively. The sensitivity of combined ultrasound and mammography, ultrasound for primary screening followed by mammography for triage, was significantly higher than mammography alone (p=0.008 and p=0.039). Additionally, ultrasound alone (48,323 RMB ($7,550)) and ultrasound for primary screening followed by mammography for triage (55,927 RMB ($8,739)) were the most cost-effective methods for breast cancer screening than other modalities. Conclusions: Ultrasound alone and ultrasound for primary screening and mammography are superior to mammography for breast cancer screening in high-risk Chinese women. [ABSTRACT FROM AUTHOR]

Four different types of 0–30 cm soil/sediment samples were collected from aquaculture land, farmland, industrial land and river bottom sediment in the estuary area of Dagu River, and the pollution status and sources of 7 PCB congeners were analyzed. The results showed that the mean values of Σ 7 PCBs in soil/sediments of different land use types were 5.01 ng g−1 dw for industrial land, 3.6 ng g−1 dw for estuarine sediments, 2.09 ng g−1 dw for farmland soil and 1.78 ng g−1 dw for farming land. All samples were at low pollution levels and pose little ecological risks. PCBs in the samples are mainly comprised of highly chlorinated biphenyls, and their content decreases gradually with increasing sampling depth. Based on the principal component analysis, it is concluded that the main source of PCBs in the study area is shipping activities, in addition to atmospheric transport and sedimentation sources. • Seven types of PCBs were found in the soil and sediments of different land types in the Dagu River Estuary. • The Σ 7 PCBs content of most sampling points gradually decreased with increasing depth. • PCBs not only came from shipbuilding and shipping activities in the area, but also from atmospheric transport and deposition. • The PCBs pollution in the area showed negligible ecological risks. [ABSTRACT FROM AUTHOR]

The natural Leymus chinensis grassland in western Jilin Province of NE China has been extensively degraded, thereby restricting the development of animal husbandry. Using theory of ecological stoichiometry homeostasis, this study investigated the homeostasis of L. chinensis at different degradation stages in the research area using a homeostasis model. Results showed that the degraded grassland generally had lower soil carbon (C), nitrogen (N) and phosphorus (P) compared with normal L. chinensis grassland. In particular, the degraded grassland exhibited severe shortage of P, but L. chinensis showed strong homeostasis. Except for C in the roots and leaves and P in stems, the N, P and corresponding ratios had higher homeostasis than 1, between 1.53 and 15.92. The homeostasis of N in L. chinensis was higher than that of P, and the homeostasis of C/P was higher than that of C/N and N/P. L. chinensis absorbs and uses nutritive elements in a conservative manner, which enables it to remain productive during changes in the external environment. Therefore, grassland degradation control should prioritize the protection of dominant species. Appropriate use of N and P fertilizers is suggested for the recovery of the seriously degraded L. chinensis grassland ecosystem. [ABSTRACT FROM AUTHOR]

Fracture-caved carbonate reservoirs (FCCRs) have been developed worldwide and contribute significantly to world's hydrocarbon reserves. Numerous models for FCCRs were proposed in the previous works. However, with more and more exploitations, some kinds of FCCRs are observed from the field geological information but not reported in the existing literature. In this paper, a novel model for FCCRs is proposed and its corresponding pressure transient analysis (PTA) and rate transient analysis (RTA) solutions are presented. This model has a wider applicability than the previous models proposed by our research team. First, the geological model is established based on the geological information. Sequentially, the simplified physical model and the mathematical model are developed. Through Laplace transformation, Duhamel's principle, and Stehfest numerical inversion, the solutions of dimensionless bottom-hole pressure and dimensionless flow rate integral are derived. Then, the RTA type curves of two-cave model are plotted to recognize the flow characteristics, which can be divided into five regimes comprehensively. In addition, two concaves are observed on the derivative curve. The RTA type curves of three-cave model are also presented. A comparison between the RTA type curves of two-cave model and three-cave model shows that when one more cave exists in the formation, one more concave will be added to the derivative curve. Furthermore, the differences between the proposed model and previous models are presented through comparing the PTA type curves. Next, the effects of key parameters on the RTA curve behavior of two-cave model are demonstrated. Results indicate that the RTA curves are controlled by the friction and fluctuation coefficients, mobility, storage ratio, region radius, and cave storage constant. The friction and fluctuation coefficients influence the depth of first concave, while the storage ratio, region radius, and cave storage constant dominate the middle flow period, and the mobility has an impact on the boundary dominated flow regime. Finally, the application of proposed model on a field well from Shunbei Oilfield, western China is presented to calibrate its accuracy and reliability. ∙ A novel model for fracture-caved carbonate reservoirs is established. ∙The rate transient characteristics of this model is conducted. ∙Sensitivity studies of some key parameters on the pressure response are conducted. ∙An application of proposed model on a field well is presented. [ABSTRACT FROM AUTHOR]

The bead-on-a-string structure has been widely observed in many fractured vuggy carbonate reservoirs, especially in Shunbei oilfield, northwestern China. To date, there are still no appropriate well test methods for analyzing these reservoirs with the vertical bead-on-a-string structure. This paper proposes a new theoretical well test model, which considers the gravity effect and the natural fractures connecting the vuggs, to analyze this kind of reservoirs. First, we establish the mathematical model of fractured vuggy reservoirs with the vertical bead-on-a-string structure. With the different outer boundary conditions, Laplace transform and inversion are employed to solve the well test model. Then, the type curves for the well test model are plotted and discussed. It is found that the gravity effect can be equivalent to a type of constant pressure boundary condition. Take the three-region-two-cave as an example, type curves can be divided into nine distinct flow regimes, including wellbore storage, skin, linear flow I and II, transition flow I and II, vug storage I and II, and boundary flow. The transition flow and vug storage period are named as the vug storage effect. Subsequently, the effects of relevant parameters are studied. It is concluded that the depth of the drop during vug storage effect increases with increasing dimensionless vug storage constant C vD and dimensionless vug height z D 2 - z D 1 ; the greater dimensionless gravity effect coefficient G D leads to the smaller equivalent boundary distance of the constant pressure boundary condition; the smaller the mobility ratio M 12 , the earlier the appearance of the second linear flow regime; the larger the storage ratio ω 12 , the more distinct the vug storage effect. Finally, two field case studies are carried out by using the proposed method. The locations and volumes of the caves are estimated, which shows that the proposed theoretical well test model has a great potential of obtaining the formation and cave parameters accurately. However, there is not available data that enable an accurate description of the locations and volumes of the caves by now, the proposed model still needs further field verifications. • A new mathematical model of fractured vuggy carbonate reservoirs considering the gravity effect is established. • It is found that the gravity effect can be equivalent to a type of constant pressure boundary condition. • Two filed cases show the great potential of the proposed method to the fractured vuggy carbonate reservoirs. [ABSTRACT FROM AUTHOR]

• The proportion of inappropriate use of nirmatrelvir-ritonavir is particularly high in China. • The main types of inappropriate use were delayed initiation of treatment, no dose adjustment for moderate renal impairment, use in severe-to-critical coronavirus disease 2019 (COVID-19), presence of contra-indicated drug‒drug interactions, and prescribing without a diagnosis of COVID-19. • Improvements in how nirmatrelvir-ritonavir is prescribed are needed urgently in the Chinese hospital setting. Nirmatrelvir-ritonavir (NMVr) is a recently developed antiviral agent for treating coronavirus disease 2019 (COVID-19); however, data describing its appropriate use are scarce. This study examined the prevalence of inappropriate use of NMVr in a Chinese hospital setting. A multi-centre retrospective chart review was performed for all hospitalized patients who received NMVr between 15 December 2022 and 15 February 2023 in four university-affiliated hospitals in Hangzhou, China. A multi-disciplinary team of experts developed the evaluation criteria. A group of senior clinical pharmacists examined and verified the suitability of NMVr prescriptions. In total, 247 patients received NMVr during the study period, of which 13.4% (n =31) met all the criteria for appropriate use of NMVr. The main types of inappropriate use of NMVr were delayed initiation of treatment (n =147, 59.5%), no dose adjustment for moderate renal impairment (n =46, 18.6%), use in patients with severe-to-critical COVID-19 (n =49, 19.8%), presence of contra-indicated drug‒drug interactions with other medications (n =36, 14.6%), and prescription for patients without a confirmed diagnosis of COVID-19 (n =36, 14.6%). The proportion of inappropriate use of NMVr was particularly high in the Chinese hospital setting, highlighting the urgent need to improve the appropriate use of NMVr. [ABSTRACT FROM AUTHOR]

The binomial deliverability equation is one of the essential parameters that control the fluid production/injection rate from wells. The presence of caves and fractures introduces many difficulties related to the determination of the binomial deliverability equation of fractured caved carbonate reservoirs. In this work, a novel method for determining the binomial deliverability equation of fractured caved carbonate reservoirs is proposed for the first time. First, the detailed procedure of our method is illustrated. More specifically, the superposition principle is used to derive the expressions of (Δ p / Q). The pressure buildup data and type curve matching are employed to induce the dimensionless bottom-hole pressure solution. Sequentially, the binomial deliverability equation can be obtained through the least square method. Then, we employ our method to a simple case and compare the calculation results with the literature data to calibrate the accuracy of our method. Next, we present the applications of our method to two example wells from the Shunbei Oilfield, western China. The results show that our method is reliable. Moreover, the results also indicate that our method is more suitable for fractured caved carbonate reservoirs than the deliverability test. Meanwhile, through the calculations of massive field data and theoretical analysis, we conclude three constraints on the input parameters. They are as follows: (i) the flow rate of each production system must be incremental; (ii) the production time must satisfy t p 1 = t p 2 – t p 1 = t p 3 – t p 2 = t p 4 – t p 3 ; and (iii) the flow rate must satisfy Q 2 = 2 Q 1 , Q 3 = 3 Q 1 , Q 4 = 4 Q 1. Finally, we prove the necessity of these constraints. • A new method for determining the binomial deliverability equation of fractured caved carbonate reservoirs is proposed. • Applications of proposed method on two field wells are presented. • Three constraints on input parameters are concluded and their necessities are calibrated. [ABSTRACT FROM AUTHOR]

Introduction: The COVID-19 pandemic has significantly impacted the mental health of human beings since 2020, especially the young people and the pre-existing marginalized groups such as men who have sex with men (MSM). During the COVID-19 pandemic, the multi-country outbreak of mpox in 2022 additionally posed a significant stress on the most-affected communities (i.e., MSM). This study investigated the level of depressive symptoms and its multifaceted associated factors among Chinese young men who have sex with men (YMSM) in this unique period., Methods: In September 2022, a large-scale cross-sectional survey was conducted among YMSM aged 18-29 years across six representative provinces in China. Hierarchical regression analysis was performed to test the various types of associated factors of depressive symptoms., Results: Among the 2493 participants, 65.6 % (n = 1638) reported mild to severe depressive symptoms. The hierarchical regression analysis identified that depressive symptoms was significantly positively associated with unemployment, having substance use in the past 6 months, a higher level of MSM self-stigma, incompletion of COVID-19 vaccination, greater mpox risk perception, and presence of mpox related-like symptoms., Limitations: This study used the facility-based sampling method to recruit the participants, which may lead to selection bias., Conclusions: Chinese YMSM faced significant mental health challenges during the concurrent epidemics of COVID-19 and mpox, which was associated with their socio-economic status, risk behaviors, stigma, and multiple diseases-related variables. Proactive measures may hold promise as effective strategies for mitigating mental distress among marginalized groups during public health crises., Competing Interests: Declaration of competing interest The authors declare no conflict of interest., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Soil/sediment samples of four different land types were collected from aquaculture land, farmland, industrial land and river bottom sediment in the estuary area of Dagu River. The contents of Cr, Cu, Zn, As, Cd and Pb in 0-30 cm inner surface samples were detected, and the distribution characteristics of heavy metal content in surface soil/sediment of different land use types in the estuary area were analyzed. Local accumulation index method, potential risk index evaluation method and principal component analysis method were used to analyze the pollution status and sources of heavy metals. The results showed that the heavy metal accumulation levels in soil and sediment samples in the study area were As > Cd > Cu > Pb > Zn > Cr, and the heavy metal content exceeded the soil background value in Shandong Province, but the potential risks were all in a low risk state. The main sources of Cr, Zn and As are transportation sources and natural sources, while the main sources of Cd and Pb are agricultural., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Shuya Hu reports financial support was provided by National Natural Science Foundation of China. Shuya Hu reports financial support was provided by Natural Science Foundation of Qingdao. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The genes of Wnt/β-catenin pathway may have potential roles in fat accumulation of Non-traumatic osteonecrosis of the femoral head (ONFH), but the effects of their variants in the pathway on ONFH development have been remained unclear. To explore the potential roles of the variants in the development of ONFH, we completed the investigation of the paired interactions as well as their related biological functions of 17 variants of GSK3β, LRP5, and FRP4 genes etc. in the pathway. The genotyping of the 17 variants were finished by MASS ARRAY PLATFORM in a 560 ONFH case-control system. The association of variants interactions with ONFH risk and clinical traits was evaluated by logistic regression analysis etc. and bioinformatics technology. The results showed that the genotype, allele frequency, and genetic models of Gsk3β rs334558 (G/A), SFRP4 rs1052981 (A/G), and LRP5 rs312778 (T/C) were significantly associated with the increased and decreased ONFH risk and clinical traits, respectively (P < 0.001-0.0002). Particularly, the paired interactions of six variants as well as eight variants also showed statistically increased and decreased ONFH risk, bilateral hip lesions risk and stage IV risk of ONFH, respectively (P < 0.044-0.004). Our results not only at the first time simultaneously showed exact serum lipid disorder and abnormal platelet function of ONFH in the same study system with the 17 variants polymorphisms of Wnt/β-catenin pathway but also shed light on the variants closely intervening the lipid disorder and abnormal coagulation of ONFH., (© 2024. The Author(s).)

Background: The worldwide human monkeypox (mpox) outbreak in 2022 mainly affected men who have sex with men (MSM). In China, young men who have sex with men (YMSM) were at a potential high risk of mpox infection due to their sexual activeness and the eased COVID-19 restrictions at the end of 2022., Objective: This study aimed to investigate the behavioral intention of receiving mpox vaccination and undergoing mpox testing in 4 different scenarios and explore their associations with background and behavioral theory-related factors among Chinese YMSM., Methods: An online cross-sectional survey was conducted among YMSM aged 18-29 years from 6 representative provinces of China in September 2022. Participants recruited (recruitment rate=2918/4342, 67.2%) were asked to self-administer an anonymous questionnaire designed based on prior knowledge about mpox and classic health behavior theories. Data on the participants' background, mpox knowledge and cognition, mpox vaccination and testing cognition, and the behavioral intention of receiving mpox vaccination and undergoing mpox testing were collected. Descriptive analysis and univariate and multivariate linear regressions were performed. Geodetector was used to measure the stratified heterogeneity of behavioral intention., Results: A total of 2493 YMSM with a mean age of 24.6 (SD 2.9) years were included. The prevalence of having a behavioral intention of receiving mpox vaccination ranged from 66.2% to 88.4% by scenario, varying in epidemic status and cost. The prevalence of having an mpox testing intention was above 90% in all scenarios regardless of the presence of symptoms and the cost. The positive factors related to vaccination intention included mpox knowledge (b a =0.060, 95% CI 0.016-0.103), perceived susceptibility of mpox (b a =0.091, 95% CI 0.035-0.146), perceived severity of mpox (b a =0.230, 95% CI 0.164-0.296), emotional distress caused by mpox (b a =0.270, 95% CI 0.160-0.380), perceived benefits of mpox vaccination (b a =0.455, 95% CI 0.411-0.498), self-efficacy of mpox vaccination (b a =0.586, 95% CI 0.504-0.668), and having 1 male sex partner (b a =0.452, 95% CI 0.098-0.806), while the negative factor was perceived barriers to vaccination (b a =-0.056, 95% CI -0.090 to -0.022). The positive factors related to testing intention were perceived severity of mpox (b a =0.283, 95% CI 0.241-0.325), perceived benefits of mpox testing (b a =0.679, 95% CI 0.636-0.721), self-efficacy of mpox testing (b a =0.195, 95% CI 0.146-0.245), having 1 male sex partner (b a =0.290, 95% CI 0.070-0.510), and having in-person gatherings with MSM (b a =0.219, 95% CI 0.072-0.366), while the negative factor was emotional distress caused by mpox (b a =-0.069, 95% CI -0.137 to -0.001)., Conclusions: Among Chinese YMSM, the intention of undergoing mpox testing is optimal, while the mpox vaccination intention has room for improvement. A future national response should raise YMSM's mpox knowledge, disseminate updated information about mpox and preventive measures, improve preventive service accessibility and privacy, and provide advice on positively coping with the associated emotional distress., (©Sitong Luo, Kedi Jiao, Yuhang Zhang, Yutong Xu, Jingtao Zhou, Siwen Huang, Yan Li, Yongkang Xiao, Wei Ma, Lin He, Xianlong Ren, Zhen Dai, Jiaruo Sun, Qingyu Li, Feng Cheng, Wannian Liang. Originally published in JMIR Public Health and Surveillance (https://publichealth.jmir.org), 19.03.2024.)

The 2022 multi-country mpox outbreak raised public concern globally. Self-isolation and informing close contacts after developing mpox-related symptoms are critical measures in controlling the outbreak. This study investigated behavioral intentions of self-isolation and informing close contacts after developing mpox-related symptoms and associated factors among young men who have sex with men (YMSM) aged 18-29 years in China. The cross-sectional study was conducted among 2493 YMSM in six provincial regions in China from September 10th to 30th, 2022. Descriptive and logistic analyses were applied, using the intentions of self-isolation and informing close contacts after developing mpox-related symptoms as binary outcomes. The mean age of the participants was 24.6 (SD = 2.9) years. The prevalence of having intentions of self-isolation and informing close contacts after developing mpox-related symptoms was 88.6% (95% CI: 87.3%-89.9%) and 84.9% (95% CI: 83.5%-86.3%). Participants who were employed (adjusted odds ratio (AOR) = 1.474, 95% CI: 1.035-2.097; AOR = 1.371, 95% CI:1.002, 1.876), had higher mpox knowledge scores (AOR = 1.474, 95% CI: 1.035-2.097; AOR = 1.371, 95% CI: 1.002-1.876), and had higher perceived threats of mpox (AOR = 1.079, 95% CI: 1.030-1.130; AOR = 1.045, 95% CI: 1.002-1.090) were more likely to intend to self-isolate and inform close contacts. Participants who had MSM in-person gatherings in the past 6 months were more likely to intend to self-isolate (AOR = 1.392, 95% CI: 1.066-1.208). Participants with higher depression scores (AOR = 0.968, 95% CI: 0.948-0.989) and self-stigma (AOR = 0.975, 95% CI: 0.954-0.997) were less likely to intend to self-isolate and inform close contacts, respectively. Self-isolation and informing close contacts when developing disease-related symptoms are acceptable measures in response to mpox in China. Strengthening targeted risk communication and self-efficacy, raising disease knowledge, providing mental support, and reducing stigma toward the affected community are warranted., (© 2024 Wiley Periodicals LLC.)

Background: We aimed to assess the performance of the risk assessment questionnaire and fecal immunochemical test (FIT) in a population-based colorectal cancer (CRC) screening program to provide timely evidence for tailored screening strategies in China., Methods: This analysis was conducted using data from Beijing Cancer Screening Prospective Cohort Study (BCSPCS). A risk assessment questionnaire and FIT were selected as the primary screening methods, and participants with any positive results were referred to undergo a diagnostic colonoscopy., Results: From 2015 to 2020, 148,636 Beijing residents aged 40-69 years were invited from designated communities, with 147,807 finishing the risk assessment questionnaire and 115,606 (78.2%) completing the FIT. Among the 42,969 (29.1%) high-risk CRC participants, 23,824 (55.4%) underwent colonoscopy. One year after enrollment, all subjects were linked to the Beijing Cancer Registry (BCR) database and 241 cases of CRC were confirmed. The CRC incidence rate was 58.2/100,000 for the low-risk arm and 418.9/100,000 for the high-risk arm. For participants who underwent colonoscopy, 91 CRC cases were detected, with a detection rate of 91.9% and 63.7% of them were early-stage cases. Furthermore, the sensitivities of utilizing the risk assessment questionnaire alone, FIT alone, combined risk assessment questionnaire and FIT were 75.7%, 50.1%, and 95.1%, and the specificities were 75.3%, 87.3%, and 70.7%, respectively., Conclusion: The Beijing CRC screening program can effectively detect early-onset CRC; however, the compliance with colonoscopy still needs to be improved., (© 2023. BioMed Central Ltd., part of Springer Nature.)

Background: Previous studies have identified a series of specific adverse pregnancy outcomes (APOs) linked with temperature extremes. Most of them focus on preterm birth, low birth weight, and stillbirth. Other possible adverse outcomes were under-researched. This study aimed to investigate the impact of ambient temperature on maternal complications, white blood cell count (WBC), newborn hearing, and neonatal jaundice., Methods: A total of 418 participants were recruited from Fuzhou Maternity & Child Healthcare Hospital in 2016. Participants were invited to fill out a structured questionnaire. The gridded near-surface air temperatures at a resolution of 0.1°* 0.1° for Fuzhou were extracted from a published dataset. Meteorological data and PM 2.5 were extracted based on participants' residential addresses using R packages "ncdf4" and "raster." Multivariate logistic regression models were used to quantify the effects of ambient temperature on APOs after controlling for confounders., Results: Overall, there were 107 APOs, accounting for 25.6% of all participants. Every 1°C increase in mean temperature was associated with a 10.0% increase in APOs (aOR = 1.100, 95%CI 1.006-1.203) during the period of early pregnancy. However, negative associations were observed in the middle pregnancy period, and a 1°C increase in mean temperature was associated 8.8% decrease in APOs (aOR = 0.912, 95%CI 0.846-0.982). Diurnal temperature variation had a significant impact on APOs in the third trimester. Infant jaundice was negatively associated with temperature exposure in the middle and late pregnancy periods. The risk of neonatal jaundice increased at lag weeks 2-9 in the first trimester, with the greatest lagged effect (aOR = 1.201, 95%CI 1.020-1.413) observed at lag week 3. A 1°C increase in mean temperature led to a 29.6% (aOR = 1.296, 95%CI 1.019-1.649) increase in high WBC. A 1°C increase in temperature variation was associated with more than two times (aOR = 2.469, 95%CI 1.001-6.089) increase of high WBC in the first trimester and about five times (aOR = 4.724, 95%CI 1.548-14.409) increase in the third trimester., Conclusion: Ambient temperature affects neonatal jaundice, newborn hearing loss, and infections during pregnancy. In addition to the identified epidemiologic link and susceptible exposure windows, there is a need to understand the underlying biological mechanisms for better recommendations for climate change adaptation policies., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Lin, Yang, Nuermaimaiti, Ye, Liu, Zhang, Chen, Li, Wu, Liu, Xu, Xia and Xiang.)

To assess the health of river ecosystems, it is essential to quantify the ecological risk of heavy metals in river sediments and the structure of microbial communities. As important tributaries of the Tuo River in the upper reaches of the Yangtze River, the Mianyuan River and the Shiting River, are closely related to the economic development and human daily life in the region. This study assessed the ecological risks of heavy-metal-polluted river sediments, the heavy-metal-driven bacterial communities were revealed, and the relationships between the ecological risks and the identical bacterial communities were discussed. The Cd content was significantly greater than the environmental background value, leading to a serious pollution and very high ecological risk at the confluence of the two rivers and the upper reaches of the Mianyuan River. Microbial community analysis showed that Rhodobacter, Nocardioides, Sphingomonas, and Pseudarthrobacter were the dominant bacterial genera in the sediments of the Shiting River. However, the dominant bacterial genera in the Mianyuan River were Kouleothrix, Dechloromonas, Gaiella, Pedomicrobium, and Hyphomicrobium. Mantel test results showed (r = 0.5977, P = 0.005) that the Cd, As, Zn, Pb, Cr, and Cu were important factors that influenced differences in the distribution of sediment bacterial communities Mianyuan and Shiting rivers. A correlation heatmap showed that heavy metals were negatively correlated for most bacterial communities, but some bacterial communities were tolerant and showed a positive correlation. Overall, the microbial structure of the river sediments showed a diverse spatial distribution due to the influence of heavy metals. The results will improve the understanding of rivers contaminated by heavy metals and provide theoretical support for conservation and in situ ecological restoration of river ecosystems., (© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)

Previous studies investigating the characteristics of imported cases were mostly limited to a certain province/city or a specific sub-group during a certain period with a small sample size, which may not provide an overall picture of the characteristics of imported cases. In this scoping literature review, we comprehensively synthesized the epidemiological characteristics of overseas imported COVID-19 cases into China by retrieving six literature databases, with aims to provide implications for more targeted control, prevention, and medical treatment of this disease. After dropping duplicates and reviewing titles, abstracts, and full-texts, 50 articles were included in the review finally, including 26 (52%) articles in English and 24 (48%) articles in Chinese. According to the type of data sources, the 50 studies were divided into three categories: 13 (26%) articles using data sourced from the Chinese Infectious Diseases Online Reporting System, 15 (30%) articles using data from the websites of national/local health departments, and 22 (44%) articles using hospital admission data. Most of the overseas imported COVID-19 cases were young and middle-aged Chinese students and businessmen returning from the United States, Europe, and some neighboring countries. Airport routine health screening measures could not identify COVID-cases effectively, although scheduled multiple nucleic acid tests were required before boarding. Almost all imported cases were identified during the hotel quarantine period. Although a large proportion of imported cases were asymptomatic or with mild symptoms in the published literature, they may be due to participant selection bias. The exact proportion of asymptomatic cases may need to be further investigated especially through population-based large-scale studies., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Zhang, Chen, Li, Yang, Chen, Lin, Xiao, Ma, Wu, Liu, Xu and Xiang.)

Seedless fruits are highly marketable because they are easier to eat than fruits with seeds. 'Shijiwuhe' is a seedless pear cultivar that is a mutant derived from an F1 hybridization population ('Bartlett' x 'Yali'). Little is known about the key genes controlling seedless pear fruit. In this study, field experiments revealed that seedless 'Shijiwuhe' pear was not due to parthenocarpy, and that it was self-incompatible. Single nucleotide polymorphisms (SNPs), small insertions and deletions (InDels) and structural variations (SVs) were characterized using DNA sequencing data between 'Shijiwuhe' and parental cultivars. A total of 1498 genes were found to be affected by SV and over 50% of SVs were located in promoter regions. Transcriptome analysis was conducted at three time points (4, 8, and 12 days after cross-pollination) during early fruit development of 'Shijiwuhe', 'Bartlett', and 'Yali'. In total, 1438 differentially expressed genes (DEGs) were found between 'Shijiwuhe' and parental cultivars 'Bartlett' and 'Yali'. We found 1193 SVs that caused differential expression of genes at 4 DACP. Among them, over 100 genes were in pathways related to seed nutrition and energy storage and 41 candidate genes encoded several important transcription factors, such as MYB, WRKY, NAC, and bHLH, which might play important roles in seed development. The qRT-PCR results also confirmed that the candidate genes with SVs showed differential expression between 'Shijiwuhe' pear and 'Bartlett' or 'Yali'. This study, which combined field experiments, SV detection, and transcriptome analysis might provide an effective way to predict the candidate genes regulating the seedless trait and important gene resources for genetic improvement of pear., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)