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Barramundi, the Asian sea bass (Lates calcarifer Bloch 1790), is a valuable aquaculture commodity, but seed survival is generally low. This study used a completely randomized design (two factors; 16 treatments; 3 replicates) to examine the effect of adding vitamin B complex and phosphorus to the rearing media on the survival, stress resistance, and phosphorus content of barramundi larvae. Barramundi larvae aged two days were stocked at a density of 25 fish L-1 in 40 L green plastic basins (30 L water) and reared for 30 days with natural feed (Chlorella and rotifers). The factor I was vitamin B complex with 4 levels: 0, 75, 150, and 225 mg L-1. Factor II was phosphorus with four levels: 0, 0.5, 1.0, and 1.5 mg L-1. Commercially available B vitamin complex and phosphorus mineral supplements were dissolved in water and added to the larval-rearing media. Analysis of variance showed that the combination of vitamin B complex and phosphorus treatments had a significant effect (P < 0.01) on survival, stress resistance, and phosphorus uptake. Survival and phosphorus content in barramundi larvae were highest with a combination of 150 mg L-1 vitamin B complex and 1.0 mg L-1 phosphorus and lowest in control (0 mg L-1 of both additives). [ABSTRACT FROM AUTHOR]

Gastric cancer is one of the most common malignant gastrointestinal tumors whose morbidity and mortality account for the second and third place respectively in malignant tumors in China. As an important participant in tumor biology, the abnormal expression of long non-coding RNA (lncRNAs) in cancer cells is closely related to the occurrence and development of tumors and plays the role of oncogenes or tumor suppressor genes. In this study, we identified a novel lncRNA NFIA antisense RNA 1 (NFIA-AS1) and explored its role and clinical significance in gastric cancer. Real-time quantitative PCR was performed to detect the expression of NFIA-AS1 in tumor tissues and corresponding normal tissues from 42 pairs of gastric cancer samples. The lower expression of NFIA-AS1 was significantly associated with larger tumor size, lower histological grade, and advanced TNM stage. Kaplan-meier analysis showed that NFIA-AS1 expression could be used as an independent predictor of overall survival. We also demonstrated that overexpression of NFIA-AS1 significantly inhibited the proliferation of gastric cancer cells through affecting p16 levels. In conclusion, our results suggest that the lncRNA NFIA-AS1 may play the role of tumor suppressor gene, and serve as a biomarker for prognosis or progression of gastric cancer. [ABSTRACT FROM AUTHOR]