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Soil fungi are ecologically important as decomposers, pathogens, and symbionts in nature. Understanding their biogeographic patterns and driving forces is pivotal to predict alterations arising from environmental changes in ecosystem. Dissolved organic matter (DOM) is an essential resource for soil fungi; however, the role of its quality in structuring fungal community patterns remains elusive. Here using Illumina MiSeq sequencing, we characterized total fungi and their functional groups in 45 soil samples collected from a 1500-km sampling transect through semi-arid regions in northern China, which are currently suffering great pressure from climate change. Total fungi and their functional groups were all observed to exhibit significant biogeographic patterns which were primarily driven by environmental variables. DOM quality was the best and consistent predictor of diversity of both total fungi and functional groups. Specifically, plant-derived DOM was associated with greater diversity relative to microbe-dominated origins. In addition, fungal diversity linearly increased with increases in degree of humification in DOM. Similarly, among all measured environmental variables, DOM quality had the strongest effects on the community composition of total fungi and functional groups. Together, our work contributes to the factors underlying fungal biogeographic patterns and adds detail to the importance of DOM quality in structuring fungal communities. [ABSTRACT FROM AUTHOR]

It is of great interest to elucidate the biogeographic patterns of soil microorganisms and their driving forces, which is fundamental to predicting alterations in microbial-mediated functions arising from environment changes. Although dissolved organic matter (DOM) represents an important resource for soil microorganisms, knowledge of how its quality affects microbial biogeography is limited. Here, we characterized soil bacterial communities and DOM quality in 45 soil samples collected from a 1500-km sampling transect through semi-arid regions in northern China which are currently suffering great pressure from climate change, using Illumina Miseq sequencing and fluorescence spectroscopy, respectively. We found that DOM quality (i.e. the source of DOM and the humification degree of DOM) had profound shaping influence on the biogeographic patterns exhibited by bacterial diversity, community composition and association networks. Specifically, the composition of bacteria community closely associated with DOM quality. Plant-derived DOM sustained higher bacterial diversity relative to microbial-derived DOM. Meanwhile, bacterial diversity linearly increased with increasing humification degree of DOM. Additionally, plant-derived DOM was observed to foster more complex bacterial association networks with less competition. Together, our work contributes to the factors underlying biogeographic patterns not only of bacterial diversity, community composition but also of their association networks and reports previously undocumented important role of DOM quality in shaping these patterns. [ABSTRACT FROM AUTHOR]

Plastic mulching is suspected to be a significant source of microplastics in terrestrial environments owing to its intensive application and improper disposal. However, there has been a comparative lack of studies examining this hypothesis. In this study, the occurrence of macroplastics in agricultural soils was investigated by analysing 384 soil samples collected from 19 provinces across China. Additionally, the abundance of microplastics was investigated in potential hotspots that have carried out plastic mulching for over 30 years. Macroplastic concentrations in the soil samples ranged from 0.1 to 324.5 kg/ha, with an average of 83.6 kg/ha; the concentrations were higher in western China than in eastern China. A highly significant linear correlation (R 2 = 0.61) was found between the consumption of mulching film and the plastic residue in soils, indicating plastic film mulching may be a major source of macroplastics. The abundances of microplastic particles increased over time in the locations where plastic mulching was continuously employed, with concentrations of 80.3 ± 49.3, 308 ± 138.1, and 1075.6 ± 346.8 pieces/kg soil in fields with 5, 15, and 24 y of continuous mulching, respectively. Fourier transform infrared analyses revealed that the composition of the microplastics matched that of the mulching films, suggesting the microplastic particles originated from the mulching films. These findings confirm that plastic mulching is an important source of macroplastic and microplastic contamination in terrestrial environments. Further studies to investigate the microplastic hazards in soils are thus necessary. Image 1 • Macroplastics in 384 soil samples from 19 provinces across China were investigated. • Macroplastics concentrations are greater in western China than in eastern China. • Significant correlation was observed between mulching mass and plastic residues. • The abundances of microplastics increased with continuous plastic mulching. • Plastic mulching was the direct source of macro/micro plastics in soils. Plastic mulching contributes significantly to microplastics and macroplastics in agricultural soils. [ABSTRACT FROM AUTHOR]

The ubiquitous presence of microplastics in aquatic environments has recently drawn considerable attention due to their potential threat to the entire ecosystem. The colonization of bacterial communities on microplastics is an important ecological linkage for microplastics in aquatic ecosystems, which is yet poorly understood. In this study, microplastic particles were sampled in two urbanized rivers in Jiaxing, Zhejiang, China, and the differences between bacterial assemblages colonizing microplastics and planktonic bacteria were estimated. Results from high-throughput sequencing showed that the bacterial communities on microplastics were less rich and diverse compared to those from the freshwater samples, with a significantly distinct taxonomic composition. The predicted functional profiles also indicated significant differences between microplastic and water samples. The functions related to biofilm formation and human diseases were relatively higher for the bacterial communities on the microplastics. Network analyses suggested that microplastic bacterial communities possessed higher average path length, clustering coefficient, and modularity compared to those in water samples. Additionally, quantitative PCR results showed microplastics selectively enriched antibiotic resistant genes (ARGs), and a good-fit correlation between ARG profiles and bacterial community composition was observed. The relative abundances of integron-integrase gene classes 1 and 2 were greater on microplastics, potentially suggesting a higher level of horizontal gene transfer. Findings of this study suggested microplastics are a novel microbial niche and may serve as hotspots for microbial interaction, potentially increasing risks to freshwater ecosystems and human health. Image 1 • Distinct bacterial community structure was observed on microplastics. • Predicted functional profiles between water and MPs were significantly different. • MPs selectively enriched antibiotic resistant genes with distinct ARG profiles. • A good-fit correlation between ARG profile and bacterial composition was obtained. • Microplastics may serve as hotspots for horizontal gene transfer. [ABSTRACT FROM AUTHOR]

The terrestrial environment is both a critical source and sink for microplastics (MPs). However, further efforts into the risk assessment, management, and mitigation activities of MPs in the terrestrial environment were limited by the scant data on their occurrence. In this study, we investigated the co-occurrence and correlations of light MPs and phthalate esters (PAEs) in the soils of China's hotspots and non-hotspot regions. Light MPs and PAEs were detected in all agricultural and urban soils (n = 125). In soils from hotspots (Shihezi, Xinjiang) where intense plastic mulching was used, the concentrations of MPs and phthalate diesters (di-PAEs) were 650-36,450 pcs kg -1 and 55.60-1236.64 μg kg -1 , respectively. In hotspots but not in non-hotspot regions of China, a positive correlation between MPs and PAEs was established, suggesting PAEs may serve as an indicator of MP contamination in hotspots. High quantities of MPs (1143-5911 pcs kg -1 ) and PAEs (67.3-1236.64 μg kg -1 ) were also detected in urban park soils, demonstrating a need for future research on MP in urban soils. In addition, the ubiquitous co-occurrence of MPs and PAEs in all 125 investigated soils revealed that potential joint toxicity, co-transformation, and co-transportation of MPs and PAEs should not be disregarded., Competing Interests: Declaration of competing interest The author has no conflicts of interest to declare., (Copyright © 2022. Published by Elsevier B.V.)

Background: The Qinghai-Tibetan Plateau represents one of the most important component of the terrestrial ecosystem and a particularly vulnerable region, which harbouring complex and diverse microbiota. The knowledge about their underground microorganisms have largely been studied, but the characteristics of rhizosphere microbiota, particularly archaeal communities remains unclear., Results: High-throughput Illumina sequencing was used to investigate the rhizosphere archaeal communities of two native alpine trees (Picea crassifolia and Populus szechuanica) living on the Qinghai-Tibetan Plateau. The archaeal community structure in rhizospheres significantly differed from that in bulk soil. Thaumarchaeota was the dominant archaeal phylum in all soils tested (92.46-98.01%), while its relative abundance in rhizospheres were significantly higher than that in bulk soil. Ammonium nitrogen, soil organic matter, available phosphorus and pH were significantly correlated with the archaeal community structure, and the deterministic processes dominated the assembly of archaeal communities across all soils. In addition, the network structures of the archaeal community in the rhizosphere were less complex than they were in the bulk soil, and an unclassified archaeal group (Unclassified_k_norank) was identified as the keystone species in all archaeal networks., Conclusions: Overall, the structure, assembly and co-occurrence patterns of archaeal communities are significantly affected by the presence of roots of alpine trees living on the Qinghai-Tibetan Plateau. This study provides new insights into our understanding of archaeal communities in vulnerable ecosystems.

Microplastics, as an emerging pollutant of global importance, have been well documented in aquatic ecosystems. However, little is known about the effects of microplastics on agroecosystems, particularly for soil microbial communities. Herein, microplastics collected from cotton fields in Xinjiang, China, were analysed with a scanning electron microscope (SEM) and high-throughput sequencing to investigate the attached bacterial communities. Microplastic surfaces, especially pits and flakes, were colonized by various microorganisms, suggesting active hydrolysis of plastic debris. The bacterial communities colonizing microplastics were significantly different in structure from those in the surrounding soil, plant litter and macroplastics. In addition, statistical analysis of differentially abundant OTUs showed that microplastics serve as a "special microbial accumulator" in farmland soil, enriching some taxa that degrade polyethylene, such as Actinobacteria, Bacteroidetes and Proteobacteria. Co-occurrence network analysis revealed that the biotic interactions between microorganisms on microplastics are as complex as those in soil, and Acidobacteria, Chloroflexi, Gemmatimonadetes, and Bacteroidetes are considered keystone species in bacterial communities. Collectively, the findings imply that microplastics acted as a distinct habitat for bacteria in farmland soil, which increases our understanding of microplastic pollution., (Copyright © 2019. Published by Elsevier B.V.)