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• Six diverse railway lines were inspected by the GPR, proving the GPR application feasibility for diverse railway line conditions. • Accuracy of the GPR-based fouling index is correlated with sieving-based fouling index. • By comparing the sensitivity of different indicators, the suitable GPR signal processing means is obtained. • Fouling composition is proved as a decisive factor for GPR-based fouling level evaluation, especially the iron ore. Ballast layer condition should be more regularly and accurately inspected to ensure safe train operation; however, traditional inspection methods cannot sufficiently fulfil this task. This paper presents a method of ground penetrating radar (GPR) application to reflect ballast layer fouling levels under diverse field conditions (annual gross passing load, cleaning and renewal year, fouling composition and transportation type). The results show that the GPR-based inspection method can assess the ballast layer fouling level with a 1–7% difference from the traditional sieving results. Fouling composition (especially metal materials) has a great effect on the GPR signals, thus affecting the inspection accuracy of ballast layer fouling level. Developing diverse GPR-based fouling indicators (by distinguishing different GPR signal features) can improve the GPR inspection applicability to the diverse field conditions. [ABSTRACT FROM AUTHOR]

Bioaerosols are widely distributed in urban air and can be transmitted across the atmosphere, biosphere, and anthroposphere, resulting in infectious diseases. Automobile air conditioning (AAC) filters can trap airborne microbes. In this study, AAC filters were used to investigate the abundance and pathogenicity of airborne microorganisms in typical Chinese and European cities. Culturable bacteria and fungi concentrations were determined using microbial culturing. High-throughput sequencing was employed to analyze microbial community structures. The levels of culturable bioaerosols in Chinese and European cities exhibited disparities (Analysis of Variance, P < 0.01). The most dominant pathogenic bacteria and fungi were similar in Chinese (Mycobacterium : 18.2–18.9 %; Cladosporium : 23.0–30.2 %) and European cities (Mycobacterium : 15.4–37.7 %; Cladosporium : 18.1–29.3 %). Bartonella , Bordetella , Alternaria, and Aspergillus were also widely identified. BugBase analysis showed that microbiomes in China exhibited higher abundances of mobile genetic elements (MGEs) and biofilm formation capacity than those in Europe, indicating higher health risks. Through co-occurrence network analysis, heavy metals such as zinc were found to correlate with microorganism abundance; most bacteria were inversely associated, while fungi exhibited greater tolerance, indicating that heavy metals affect the growth and reproduction of bioaerosol microorganisms. This study elucidates the influence of social and environmental factors on shaping microbial community structures, offering practical insights for preventing and controlling regional bioaerosol pollution. [Display omitted] • Chinese urban had higher levels of culturable bioaerosol pollution than Europe. • Pathogens Mycobacterium and Cladosporium were highly prevalent in China and Europe. • Bacteria in China showed higher MGEs abundance and biofilm-forming capacity. • Environment and socio-economic factors shaped microbial community structure. • Heavy metals widely present in ACC influence microbial growth and reproduction. [ABSTRACT FROM AUTHOR]