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Forecasting carbon dioxide (CO2) emissions is necessary to estimate their climate impact and the required mitigation efforts. However, existing scenario frameworks are not consistent and limit the comparability of projection studies. In this study, the "Stochastic Impacts by Regression on Population, Affluence and Technology" model was combined with the shared socioeconomic pathways (SSPs) defined by the Intergovernmental Panel on Climate Change. Model forecasts were calculated for each SSP from 2020 to 2060 in Qinghai Province, an underdeveloped, carbon-sink-rich region in China. Total CO2 emissions in Qinghai Province consistently exhibited an upward then downward trend, with large differences between SSPs in the peak date and maximum value of total CO2 emissions. Considering the current socioeconomic development status and total carbon emission trends in Qinghai Province, SSP1 and SSP2 were the most suitable pathways, providing balance between carbon emissions reduction and population well-being. [ABSTRACT FROM AUTHOR]

In this study, a Long Short-Term Memory (LSTM) network approach is employed to evaluate the prediction performance of PM2.5 in the Beijing–Tianjin–Hebei region (BTH). The proposed method is evaluated using the hourly air quality datasets from the China National Environmental Monitoring Center, European Center for Medium-range Weather Forecasts ERA5 (ECMWF-ERA5), and Multi-resolution Emission Inventory for China (MEIC) for the years 2016 and 2017. The predicted PM2.5 concentrations demonstrate a strong correlation with the observed values (R2 = 0.871–0.940) in the air quality dataset. Furthermore, the model exhibited the best performance in situations of heavy pollution (PM2.5 > 150 μg/m3) and during the winter season, with respective R2 values of 0.689 and 0.915. In addition, the influence of ECMWF-ERA5's hourly meteorological factors was assessed, and the results revealed regional heterogeneity on a large scale. Further evaluation was conducted by analyzing the chemical components of the MEIC inventory on the prediction performance. We concluded that the same temporal profile may not be suitable for addressing emission inventories in a large area with a deep learning method. [ABSTRACT FROM AUTHOR]

With the aim of effectively preventing and controlling systemic risk, by stimulating the advancement of the green bond market, it is significant and imperative to help investors and policymakers adopt more effective measures, which will ensure them to maximize profit. We construct VAR, DCC-GARCH and Copula-CoVaR models, and study the spillover effect between the green bond market and traditional bond market from the three perspectives of mean spillover, volatility spillover and extreme risk spillover using the data on daily closing prices of green bond market and traditional bond market indices. The research findings of this paper are as follows: (1) There are three spillover effects of mean value, volatility and extreme risk among the green bond market, corporate bond market, enterprise bond market and conventional bond market. (2) From the perspective of mean spillover between markets, only the mean spillover between the conventional bond market and the green bond market is bidirectional, and there is the profoundest impact of spillover from the green bond market to the conventional bond market. (3) As far as the volatility spillover between markets is concerned, the volatility spillover between the three traditional bond market and the green bond markets are all positive. The volatility spillover between the conventional bond market and the green bond market is the largest, which is particularly obvious in the first half of 2018 and the first half of 2020. (4) In terms of inter-market extreme risk spillover, the risk spillover between the green bond market and the traditional bond market is positive. The green bond market contributes more to the risk spillover of the enterprise bond market, and it has a time-varying risk spillover effect on the traditional bond market. [ABSTRACT FROM AUTHOR]

Background: Whether medical insurance impacts the timely diagnosis in chronic kidney disease (CKD) children is unknown. We aim to examine the extent to which insurance is associated with access to timely diagnosis and different stages of CKD among a large population of children in China. Methods: A retrospective, cross-sectional study based on China's national hospitalized record database was carried out. Children aged 0–17 years diagnosed as CKD stages 1–5 between June 1, 2013, and December 31, 2018, were included. A diagnosis of advanced CKD stage (CKD stage 4 or 5) was the primary outcome. Multivariable logistic regression model adjusted for age, sex, cross-regional hospitalization, year of diagnosis, and cause of CKD was used to assess the association between insurance status and the stage of CKD when diagnosed. Results: A total of 10,256 children (median [interquartile range, IQR] age, 12.4 [7.9, 15.4] years) were included. There were 4716 (46.0%) uninsured children in the included population. The insurance coverage was highest in children 13–17 years old (60.9%). The hospitalized charge was highest in stage 5 uninsured children (median [IQR], ¥13,000.89 [7640.63, 24,585.00]). More uninsured children are diagnosed in CKD stage 4 or 5 (48.1%) than insured children (47.5%). Uninsured children had higher odds (odds ratio [OR] 1.20, [95% CI, 1.08–1.32]) of receiving a diagnosis of CKD stage 4 or 5 compared with insured children. Conclusions: Lack of medical insurance was associated with a more advanced stage of CKD when diagnosed in hospitalized children. A higher resolution version of the Graphical abstract is available as Supplementary information. [ABSTRACT FROM AUTHOR]

Introduction: Neuromyelitis optica spectrum disorders (NMOSD), mainly mediated by B cells and AQP4 antibody, has a high rate of recurrence. Telitacicept is a novel drug specifically targeting the upstream signaling for the activation of B cell with its following production of autoimmune antibodies. Thus, it may be a promising approach. Our study preliminarily explored the potential safety and effectiveness of Telitacicept following plasma exchange in the treatment of recurrent NMOSD. Methods: This was a single‐center, single‐arm, open‐label study enrolling eight patients with recurrent NMOSD in China. All patients received plasma exchange three times, followed by Telitacicept 240 mg every week for 46 times. The primary endpoint was the time of first recurrence after enrollment. Secondary end points included: changes in Expanded Disability Status Scale score, Optic Spinal Impairment Scale score, Hauser Ambulation Index, number of lesions on MRI, retinal nerve fiber layer thickness measured by optical coherence tomography, latency and amplitude of visual evoked potential, titer of AQP4 antibody, and immune parameters of blood. Safety was also assessed. The study was registered with Chictr.org.cn (ChiCTR1800019427). Results: Eight eligible patients were enrolled. Relapse occurred in two patients (25%) and five patients (63%) remained relapse free after 48 weeks of treatment. The time to first recurrence was prolonged and the number of recurrences was reduced (p < 0.001, power of test = 1). One patient withdrew from the study due to low neutrophil count. No serious adverse events occurred. Conclusions: In this small, uncontrolled study, Telitacicept following plasma exchange has the potential to be a safe treatment for patients with recurrent NMOSD. It may prolong the recurrence interval and reduces the annual count of recurrences. A multicenter randomized controlled study with a larger sample is thus feasible and needed to further assess its safety and efficacy. [ABSTRACT FROM AUTHOR]

Background: Adiposity evaluated by body mass index (BMI) is associated with glycometabolism. The aim of the investigation was to explore the correlation of visceral fat area (VFA), body fat percentage (BFP), BMI and waist circumference (WC) with type 2 diabetes mellitus (T2DM) and pre-diabetes. Methods: A total of 18,458 participates underwent physical examination in Nanjing Drum Tower Hospital from January 2018 to April 2022 was included in this study. Data were collected retrospectively. Regression analysis was used to evaluate the relationship of VFA, BFP, WC and BMI with diabetes status, fasting blood glucose (FBG) and glycohemoglobin (HbA1c). Results: After fully adjusted for multiple covariates, VFA, BFP, WC and BMI in T2DM and pre-diabetes group exceeded compared with normal group. FBG was positively correlated with VFA, BFP, WC and BMI with βs of 2.221,0.306,0.606 and 0.175(p < 0.001). HbA1c was also positively correlated with the four indexes with βs of 2.645, 0.328, 0.685 and 0.255(p < 0.001). Subgroup analysis shown that FBG and HbA1c were positively correlated with VFA, BFP, BMI and WC in normal and pre-diabetes group (p < 0.001). FBG was negatively correlated with BMI in T2DM group (p = 0.023). In T2DM, there were non-linear relationships of HbA1c with VFA, BFP, WC and BMI with the inflection points for about 7%. Before the inflection point, HbA1c was positively correlated with obesity-related indicators, and it was reversed after the inflection point. In the individuals with excessive VFA and normal BMI, the risk for glycometabolism disorder exceed compared with normal VFA and normal BMI. Every per-standard deviation increasing in VFA, BFP, WC and BMI, the corresponding risk increasing of glycometabolism disorder was 16.4, 14.6, 22.6 and 22.2%. Conclusion: The study demonstrated that in adults with T2DM or prediabetes, the VFA, BFP, WC and BMI were higher than with normal glycometabolism. In pre-diabetes and normal population, there were positive correlations of HbA1c and FBG with obesity-related indicators. In T2DM with poor glycemic control (HbA1c > 7%), there might be a trend of fat loss. VFA could negatively affect glycometabolism independently from BMI. The optimum to evaluate the risk of glycometabolism disorder was WC. [ABSTRACT FROM AUTHOR]

Background: Haemolytic uraemic syndrome (HUS) is a severe syndrome that causes a substantial burden for patients and their families and is the leading cause of acute kidney injury in children. However, data on the epidemiology and disease burden of HUS in Asia, including China, are limited. We aimed to estimate the incidence and cost of HUS in China.  METHODS: Data about HUS from 2012 to 2016 were extracted from the Urban Employee Basic Medical Insurance (UEBMI) and Urban Resident Basic Medical Insurance (URBMI) databases. All cases were identified by ICD code and Chinese diagnostic terms. The 2016 national incidence rates were estimated and stratified by sex, age and season. The associated medical costs were also calculated.Results: The crude incidence of HUS was 0.66 per 100,000 person-years (95% CI: 0.35 to 1.06), and the standardized incidence was 0.57 (0.19 to 1.18). The incidence of HUS in males was slightly higher than that in females. The age group with the highest incidence of HUS was patients < 1 year old (5.08, 95% CI: 0.23 to 24.87), and the season with the highest incidence was autumn, followed by winter. The average cost of HUS was 2.15 thousand US dollars per patient, which was higher than the national average cost for all inpatients in the same period.Conclusions: This is the first population-based study on the incidence of HUS in urban China. The age and seasonal distributions of HUS in urban China are different from those in most developed countries, suggesting a difference in aetiology. [ABSTRACT FROM AUTHOR]

Background We aimed to describe the epidemiological, virological, and serological features of coronavirus disease 2019 (COVID-19) cases in people living with human immunodeficiency virus (HIV; PLWH). Methods This population-based cohort study identified all COVID-19 cases among all PLWH in Wuhan, China, by 16 April 2020. The epidemiological, virological, and serological features were analyzed based on the demographic data, temporal profile of nucleic acid test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during the disease, and SARS-CoV-2–specific immunoglobin (Ig) M and G after recovery. Results From 1 January to 16 April 2020, 35 of 6001 PLWH experienced COVID-19, with a cumulative incidence of COVID-19 of 0.58% (95% confidence interval [CI],.42–.81%). Among the COVID-19 cases, 15 (42.86) had severe illness, with 2 deaths. The incidence, case-severity, and case-fatality rates of COVID-19 in PLWH were comparable to those in the entire population in Wuhan. There were 197 PLWH who had discontinued combination antiretroviral therapy (cART), 4 of whom experienced COVID-19. Risk factors for COVID-19 were age ≥50 years old and cART discontinuation. The median duration of SARS-CoV-2 viral shedding among confirmed COVID-19 cases in PLWH was 30 days (interquartile range, 20–46). Cases with high HIV viral loads (≥20 copies/mL) had lower IgM and IgG levels than those with low HIV viral loads (<20 copies/ml; median signal value divided by the cutoff value [S/CO] for IgM, 0.03 vs 0.11, respectively [ P  < .001]; median S/CO for IgG, 10.16 vs 17.04, respectively [ P  = .069]). Conclusions Efforts are needed to maintain the persistent supply of antiretroviral treatment to elderly PLWH aged 50 years or above during the COVID-19 epidemic. The coinfection of HIV and SARS-CoV-2 might change the progression and prognosis of COVID-19 patients in PLWH. [ABSTRACT FROM AUTHOR]

The main purpose of English learning is to communicate and interact in global contexts. However, in English as a foreign language (EFL) contexts as in China, most of the students have limited interactional competence in contrast to their grammatical and structural competence. The reason is that Chinese classrooms mostly lack an interactional climate. This calls for an urgent need to develop interpersonal interaction skills by EFL teachers via appropriate strategies. To this end, this article presents an overview of nine interpersonal communication skills/strategies which are pivotal in L2 education. It also describes their definitions and related theories. Moreover, the outcomes of such strategies in aural skills are also explained. Finally, implications, research gaps, and future avenues for research are provided. [ABSTRACT FROM AUTHOR]

aim of the present study was to investigate the predictive value of transvaginal ultrasonography measurement of cervical length (CL) during the second trimester for spontaneous preterm birth. Methods: Data from 1222 women with a single fetus pregnancy, who delivered at our hospital between March 2019 and May 2020, were retrospectively analyzed. CL was measured during the second trimester, with a length of < 25 mm regarded as cervical shortening. The relationship between CL, cervical shortening, and pregnancy outcome was analyzed. Results: The incidence of spontaneous preterm birth and cervical shortening in the 1222 women was 7.3% (89/1222) and 0.33% (4/1222), respectively. The average CL during the second trimester was 37.9 ± 5.7 mm for the spontaneous preterm birth group and 39.3 ± 3.8 mm for those who gave birth at full term. Three of the four cases of cervical shortening resulted in a spontaneous preterm birth. This showed a predictive sensitivity of 3.33% and a specificity of 99.9%. Conclusion: CL measurement during the second trimester can be used as a routine test to predict spontaneous preterm birth. During the second trimester, the distribution of CL in women with single fetus pregnancies in China is different compared with other countries. Reducing the threshold of CL may improve the predictive value for preterm birth. [ABSTRACT FROM AUTHOR]

Background: The main purpose was to determine basic epidemiological data on CKD among hospitalized pediatric patients in China.Methods: Data from pediatric inpatients with CKD hospitalized from June 1, 2013 to May 31, 2017 were extracted from the electronic records of HQMS database, which includes over 14 million inpatients. Codes from the 10th revision of the International Classification of Diseases (ICD-10) were used to search the database.Results: A total of 524 primary diseases of CKD were included in this study. In all, there were 278 231 pediatric inpatients with CKD, which accounted for 1.95 % of the 14 250 594 pediatric inpatients registered in the HQMS database. The number of pediatric inpatients with CKD was 67 498 in 2013, 76 810 in 2014, 81 665 in 2015 and 82 649 in 2016, which accounted for 1.93 %, 1.93 %, 1.99 and 2.09 %, respectively, of the total population of pediatric inpatients. The etiology of CKD was secondary nephrosis in 37.95 % of cases, which ranked first and followed by CAKUT with a percentage of 24.61 %. Glomerular diseases and cystic kidney disease accounted for 21.18 and 5.07 %, respectively. Among all 278 231 patients, 6 581 (2.37 %) had a primary discharge diagnosis of CKD. The renal pathology findings of CKD showed that IgA accounted for 51.17 %.Conclusions: This study provides a descriptive analysis of the hospitalized population of pediatric CKD patients. Our study provides important, fundamental data for policy making and legislation, registry implementation and the diagnosis, treatment and prevention of CKD in China. [ABSTRACT FROM AUTHOR]

Paragonimus proliferus, a lung fluke of the genus Paragonimus, was first reported in Yunnan province, China. P. proliferus can infect Sprague-Dawley (SD) rats and cause lung damage, but there is still no direct evidence of human infection. Until now, there has been a lack of studies on P. proliferus parasitism and development in mammalian lung tissue. The aim of this study was to perform transcriptomic profiling of P. proliferus at different developmental stages. SD rats were infected with P. proliferus metacercariae obtained from crabs; worms isolated from the lungs at different time points as well as metacercariae were subjected to whole transcriptome sequencing. Overall, 34,403 transcripts with the total length of 33,223,828 bp, average length of 965 bp, and N50 of 1833 bp were assembled. Comparative analysis indicated that P. proliferus, similar to other Paragonimus spp., expressed genes related to catabolism, whereas P. proliferus-specific transcripts were related to the maintenance of cellular redox homeostasis, sensitivity to bacteria, and immune response. Transcriptional dynamics analysis revealed that genes involved in the regulation of catabolism and apoptosis had stable expression over the P. proliferus life cycle, whereas those involved in development and immune response showed time-dependent changes. High expression of genes associated with immune response corresponded to that of genes regulating the sensitivity to bacteria and immune protection. We constructed a P. proliferus developmental model, including the development of the body, suckers, blood cells, reproductive and tracheal systems, lymph, skin, cartilage, and other tissues and organs, and an immune response model, which mainly involved T cells and macrophages. Our study provides a foundation for further research into the molecular biology and infection mechanism of P. proliferus. [ABSTRACT FROM AUTHOR]

The large yellow croaker (Larimichthys crocea) is one of the most economically important marine fishes on the southeast coast of China. However, high-density cultures, inbreeding depression, and environmental pollution have resulted in L. crocea often experiencing hypoxia, which has caused huge economic losses to the L. crocea farming industry. This study aimed to improve the hypoxia tolerance of farmed L. crocea using genomic selection (GS) for breeding. In this study, 399 and 354 samples from the reference and candidate populations, respectively, were genotyped using a 55 K liquid single-nucleotide polymorphism (SNP) array. Based on two different representations (survival status and survival time), the estimated values of heritability for the hypoxia tolerance trait in L. crocea using 38,472 clean SNPs were 0.65 and 0.62, respectively. A comparison of the genomic prediction accuracy under different GS models and SNP densities showed that GBLUP was the best GS model, and the 2500 SNPs selected using a genome-wide association study (GWAS)-based strategy were essentially close to all 38,472 SNPs. Based on the established optimal GS strategy (prediction accuracy was 0.4901 ± 0.0246), genomic estimated breeding values (GEBVs) were calculated for each candidate population. The tolerance capacity tests showed a significant improvement in hypoxia tolerance in the offspring of the candidate population with the top 10% GEBVs, having an 13.40% decrease and a 34.02% increase in the median lethal concentration (LC 50) and median lethal time (LT 50), respectively, compared to the control group. These results confirmed that GS is an effective genetic breeding technique when selecting for hypoxia tolerance in L. crocea. • The heritability for the hypoxia tolerance in L. crocea were 0.65 and 0.62, respectively. • GBLUP models are more suitable for genomic prediction of hypoxia tolerance traits in L. crocea. • The 2500 SNPs selected using a GWAS-based strategy were essentially close to all 38,472 SNPs. • The heritability for the hypoxia tolerance in L. crocea were 0.65 and 0.62, respectively. [ABSTRACT FROM AUTHOR]

• An energy storage value estimation model in monopoly power markets is put forward. • Ancillary services model in the monopolized electricity market is proposed. • A novel value-drive policy is introduced and compared with two other mechanisms. • Energy storage value under different energy storage configurations is analyzed. Recent trend in increasing the penetration level of renewable energy challenges safety and stability of the power grid. Electrical energy storage (EES) has been deemed as one of the key elements needed to mitigate the fluctuant and intermittent nature of renewables, and is undergoing rapid development nowadays. However, there are still many questions regarding the benefits and drawbacks that EES technologies will present to society, as well as the economic feasibility of EES in monopoly power markets. Given this, this paper presents a grid-scale production cost model for monopoly power markets in which EES generates profits by offering both energy and ancillary services. The production costs of the power system with and without an EES power plant was compared in order to generate a value estimation. Results indicate that the value of EES is related to the unit fuel price and the start-up cost of thermal power units, the penetration level of wind power and size of the EES system. Based on these variables, a value-driven pricing mechanism for EES was put forward and compared with other policies. Economic viability analysis revealed that the pricing mechanism currently used in monopoly power markets are highly inaccurate in capturing the value generated by EES. [ABSTRACT FROM AUTHOR]

Objective: Atypical hemolytic uremic syndrome (aHUS) is associated with defective complement regulation. Anti-complement factor H (CFH) antibodies were thought to participate in the pathogenesis of aHUS. The aim of this study was to address the functions and properties of CFH autoantibodies in a Chinese Han cohort of aHUS patients.Methods: Thirty-six anti-CFH antibody-positive aHUS patients at the acute phase of the disease were involved in this study. Clinical data of the patients were collected. Anti-CFH immunoglobulin G (IgG) subclasses and antibody isotypes were detected by ELISA. Epitope mapping was performed using recombinant CFH fragments (SCRs 1-4, SCR 7, SCRs 11-14, and SCRs 19-20). Purified IgG from plasma from seven patients were used for functional analyses.Results: All patients presented with the classic triad of HUS. The anti-CFH autoantibodies mostly bound to the SCRs 19-20 domains of CFH but not the SCRs 1-4 domains. CFI cofactor activity was not disturbed by the anti-CFH antibody in any of the seven patients. Purified IgG interfered with the binding of CFH to C3b and CFH-mediated sheep erythrocyte protection in all seven patients. IgG from 4/5 (80%) patients tested inhibited the binding of CFH to glomerular endothelial cells.Conclusions: Our study suggests that the properties of CFH antibodies from patients with aHUS, including the recognition of SCRs and IgG subclasses, can influence and impair the biological role of CFH and therefore contribute to aHUS susceptibility. [ABSTRACT FROM AUTHOR]

The large yellow croaker (Larimichthys crocea) is a commercially important marine fish indigenous to the southeastern coast of China. Cultured L. crocea is frequently subjected to hypoxia which causes huge economic losses to the aquaculture industry. Therefore, it is crucial to elucidate the molecular mechanisms of hypoxia tolerance in L. crocea. Here, we combined a genome-wide association study (GWAS) and transcriptome analysis to reveal single-nucleotide polymorphisms (SNPs) and the mechanisms by which they regulate hypoxia tolerance in L. crocea. We used liquid chips to genotype 400 L. crocea individuals, obtained 120,815 high-quality SNPs, identified seven SNPs with significant suggestive association levels, and found a significant peak on chromosome 1. We then combined the potential candidate genes obtained from GWAS with the differentially expressed genes (DEGs) obtained by transcriptome sequencing. We found that the genes and processes related to glucose transport and metabolism, erythropoiesis, ion regulation, DNA replication and repair, and cell cycle were critical to hypoxia adaptation in L. crocea. We also identified the gene lias that might regulate hypoxia-inducible factor (HIF)-1α. The results of the present study provided insight into the molecular mechanisms of hypoxia tolerance in fish and may facilitate the genetic selection and breeding of hypoxia tolerance in L. crocea. • We identified candidate genes by GWAS and DEGs by transcriptome analyses. • Seven SNPs with significant suggestive association levels were identified. • Molecular mechanisms regulating hypoxia tolerance of L. crocea were elucidated. [ABSTRACT FROM AUTHOR]

The large yellow croaker (Larimichthys crocea) is an economically important fish farmed along the southeast coasts of China. In recent years, however, hypoxia has caused substantial economic losses in the L. crocea aquaculture industry. At present, the numbers of genes and molecular markers available for the selection and breeding of hypoxia tolerance traits in L. crocea remain very limited, and thus there is an urgent need to conduct breeding studies on the tolerance of this fish to hypoxia. Genome-wide association studies (GWAS) have been widely used to study the genetic basis of economically important traits in aquatic animals, and in this study, we adopted a GWAS approach to genetically characterize hypoxia tolerance in the L. crocea. A total of 1982 fish were subjected to hypoxia experiments, among which 398 individuals characterized by the most sensitive and tolerant phenotypes were genotyped. Following filtering, we obtained a total of 54,224 high-quality single-nucleotide polymorphisms (SNPs), of which four were identified as significant based on mixed linear (MLM) and Bayesian-information and Linkage-disequilibrium Iteratively Nested Keyway (Blink) model analyses. Using these four SNPs, we identified ten candidate genes, including one gene, egln2 , directly associated with hypoxia and other involved in important regulatory pathways, such as oxidative stress, ion regulation, and energy metabolism. These findings provide new insights into the genetic basis of hypoxia tolerance in L. crocea and will facilitate future breeding for marker-assisted selection and the breeding of hypoxia-tolerant L. crocea for commercial production. • The first GWAS analysis of hypoxia tolerance traits in large yellow croaker was conducted. • Four significant SNPs were repeatedly detected by MLM and Blink models. • We identified ten candidate genes, including one gene directly related to hypoxia, egln2. • This study will provide valuable information for future genome-wide selective breeding of large yellow croaker. [ABSTRACT FROM AUTHOR]

Background: The aim of this study was to analyze the long-term efficacy and safety of angiotensin-converting enzyme inhibitor (ACEi) and ACEi + angiotensin receptor blocker (ARB) treatments in a cohort of children with Alport syndrome (AS). Methods: This was a respective review of 79 Chinese children with AS who received ACEi alone or combined ACEi + ARB therapy. Results: The mean age of the pediatric patients with AS at onset of treatment was 8.6 ± 4.1 (range 1.5-16.3) years. The mean duration of follow-up was 2.5 ± 1.8 (range 0.5-7.8) years. For analysis, we separated the children into three groups according to proteinuria level before treatment, namely, <25, 25-50, and ≥50 mg/kg/day, respectively; after 1 year of treatment the proteinuria had decreased from 11.0 to 9.7 mg/kg/day, from 34.6 to 15.2 mg/kg/day, and from 73.0 to 50.0 mg/kg/day in each group, respectively. Proteinuria decreased significantly during the first 2 years of treatment and was stable from the third to fifth years of treatment. There was no statistically significant difference in the antiproteinuric effect of the ACEi and ACEi + ARB treatments in patients with severe or less severe mutations after 1 year of therapy. Five children stopped the ACEi + ARB treatment due to a decline in creatinine clearance. Conclusion: Our findings demonstrate that early and long-term ACEi and ARB treatments in children with AS is efficient and well tolerated. The antiproteinuric effect of ACEi and ARB is of equal value in children with severe and less severe mutations in the COL4An gene. [ABSTRACT FROM AUTHOR]

The presence of HPV in breast tissue and the potential causal association between human papillomavirus (HPV) and breast cancer (BC) remains controversial. The aim of the present study was to compare the HPV prevalence in BC tissues, adjacent normal breast tissues and breast benign disease tissues and to investigate the possible association between HPV and breast tumor development in Chinese women. Paraffin-embedded specimens from 187 pairs of BCs including tumor and normal breast tissue adjacent to tumors and 92 breast benign lesions between June 2009 and July 2014 were investigated by nested polymerase chain reaction (PCR) and type-specific PCR, respectively. With strictly quality control, HPV positive infection was detected in three BC tissues. No HPV positive infection was detected in all normal breast tissue adjacent to tumors and benign breast tissues. Through our detailed analysis, rare HPV infection in this study suggests that HPV might not be associated with BC progression. [ABSTRACT FROM AUTHOR]

ABSTRACT Aim: Alport syndrome (AS) is a progressive renal disease characterized by hematuria and progressive renal failure. X-linked dominant AS (XLAS) is the predominant inheritance form caused by mutations in COL4A5 gene. Attitudes toward genetic diagnosis and prenatal diagnosis for Chinese AS families were investigated. Attitudes toward genetic diagnosis and prenatal diagnosis in Chinese XLAS families were evaluated in the current study. Methods: A total of 160 XLAS patients and their 126 healthy family members in China were interviewed. After providing background knowledge counselling and education on AS, their attitudes toward genetic diagnosis and prenatal diagnosis were evaluated by multiple-choice questionnaire. Results: Majority of the respondents cared mostly about the prognosis and treatment effects of AS (89.9% vs 81.1%) since they considered that the worst outcome of XALS was renal insufficiency (92.3%). Of all the interviewees, 99.3% were interested in genetic research for the discovery of better treatments and more appropriate diagnostic tools (positive attitudes) (89.5% vs 73.2%). About 80% of the participants would accept prenatal testing and subsequent termination of pregnancy in cases of affected foetuses (boys: 86.8% and girls: 74.6%, respectively). Conclusion: Most Chinese XLAS families show positive attitudes and desire new discoveries in treatment and diagnosis. About 80% of respondents would approve prenatal testing with a desire for selective termination of pregnancy rather than predicting the health of a future child. [ABSTRACT FROM AUTHOR]

Abstract: This paper outlines an analysis of the spatial distribution of sediment production, delivery and yield in the Xihanshui River basin, South Gansu, China, using the modelling tools of SedNet (Prosser et al., 2001). This model can assess the delivery efficiency to downstream locations, as well as identifying locations with high rates of sediment production. Preliminary model experiments assist understanding of the spatial dynamics of these sediment processes and evaluation of the effectiveness of soil conservation practices since the mid-1980s. Three scenario years (dry, average and wet) from the 1983–2005 record are identified and modelled, and land use and management are represented in the model to reflect known changes since the 1980s. Results show hillslope erosion to be a dominant source of sediment supply, causing the latter to decrease ten-fold between 1984 and 1997/2000. Estimated bank erosion and floodplain deposition rates are sensitive to parameter values, but bank erosion appears less sensitive than hillslope supply to rainfall. The model can be used to assess net changes in floodplain storage; for default parameters, floodplain deposition rates are 25–200 times the rates of bank erosion depending on the climate scenario. Comparing simulation results with measured sediment yields at the three gauging stations indicates encouraging agreement in 2000. In 1984 (the wet year), the model under-predicts, suggesting that additional unmodelled sediment production processes, especially mass movement and gully erosion, may be important in wet years. Mass movement inventory data could close the gap between the high yields measured in the wet scenario year and the estimated yield due to hillslope erosion alone. In 1997 (the dry year), the model over-predicts; this suggests that the land use change parameters required to reflect the effects of conservation may not have been sufficient, implying that conservation has been generally effective, and that evidence of declining sediment yield is not simply a reflection of drier conditions. [Copyright &y& Elsevier]

• Correlation and propagation among meteorological, agricultural and groundwater droughts are firstly explored in the Yangtze River Basin and the Yellow River Basin. • The propagation time from meteorological to agricultural drought in the Yangtze River Basin is longer than that in the Yellow River Basin. • There is a weak linkage between groundwater drought and meteorological/agricultural drought in the two selected basins, especially in the Yellow River Basin. • Groundwater extraction is a noticeable factor triggering groundwater drought in the two basins. The propagation of meteorological drought in the hydrological cycle not only leads to the deficits of surface soil moisture, which results in agricultural drought, but also probably affects groundwater to trigger groundwater drought. Improving the understanding about the correlation and propagation among meteorological, agricultural and groundwater droughts is necessary to lessen the risks resulted from them. Moreover, the distinction of the correlation and propagation over the basins with different climate conditions is not yet sufficiently understood. As a case study of the humid and arid/semi-arid basins in China, the standardized precipitation index, the standardized soil moisture index and groundwater drought index based on GRACE (Gravity Recovery and Climate Experiment) are used to characterize meteorological, agricultural and groundwater drought respectively over the Yangtze River Basin with a humid climate condition and Yellow River Basin with an arid/semi-arid climate condition. The Spearman rank correlation coefficient is applied to investigate the correlation and propagation among the above three types of drought in the two basins. The results indicated that: (1) The meteorological, agricultural and groundwater droughts in the Yangtze River Basin and the meteorological and agricultural droughts in the Yellow River Basin are decreasing from April 2002 to March 2020, while the groundwater drought in the Yellow River Basin is aggravating in recent years. (2) There is a strong link between meteorological and agricultural droughts, and the propagation time from meteorological to agricultural drought in summer and autumn is shorter than that in winter and spring in the two basins. When comparing the propagation time in the two selected basins, it is longer in the Yangtze River Basin. (3) Groundwater extraction may be the main factor in the aggravation of groundwater drought in the two basins, which is different from the contributors to agricultural drought, which is mainly the propagation of meteorological drought. [ABSTRACT FROM AUTHOR]

Simple Summary: Hypoxia, which occurs frequently in aquaculture, can cause serious harm to all aspects of the growth, reproduction and metabolism of cultured fish. Due to the intolerance of Larimichthys crocea to hypoxia, Larimichthys crocea often floats head or even dies under hypoxic environment. However, the molecular mechanism of hypoxia tolerance in Larimichthys crocea has not been fully described. Therefore, the aim of this study was to explore the hub regulatory genes under hypoxic stress environment by transcriptome analysis of three key tissues (liver, blood and gill) in Larimichthys crocea. We identified a number of important genes that exercise different regulatory functions. Overall, this study will provide important clues to the molecular mechanisms of hypoxia tolerance in Larimichthys crocea. The large yellow croaker (Larimichthys crocea) is an important marine economic fish in China; however, its intolerance to hypoxia causes widespread mortality. To understand the molecular mechanisms underlying hypoxia tolerance in L. crocea, the transcriptome gene expression profiling of three different tissues (blood, gills, and liver) of L. crocea exposed to hypoxia and reoxygenation stress were performed. In parallel, the gene relationships were investigated based on weighted gene co-expression network analysis (WGCNA). Accordingly, the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that several pathways (e.g., energy metabolism, signal transduction, oxygen transport, and osmotic regulation) may be involved in the response of L. crocea to hypoxia and reoxygenation stress. In addition, also, four key modules (darkorange, magenta, saddlebrown, and darkolivegreen) that were highly relevant to the samples were identified by WGCNA. Furthermore, some hub genes within the association module, including RPS16, EDRF1, KCNK5, SNAT2, PFKL, GSK-3β, and PIK3CD, were found. This is the first study to report the co-expression patterns of a gene network after hypoxia stress in marine fish. The results provide new clues for further research on the molecular mechanisms underlying hypoxia tolerance in L. crocea. [ABSTRACT FROM AUTHOR]

Chinese cabbage is a key vegetable crop in northwest China. It is of great significance to study the evapotranspiration (ET) and crop coefficient (Kc) for agricultural water-saving management in this area. Eddy covariance (EC) was used to measure the ET and Kc of Chinese cabbage in northwest China from 1 May to 16 October 2020, in order to analyze the characteristics of these variables under plastic mulch. The results showed that the average Kc of the first crop of cabbage was higher in the middle and late stages, with values of 1.08 and 1.09 during the heading and maturity stages, respectively. The average Kc of the second crop of cabbage was higher in the middle stage, with values of 1.10 and 1.13 during the rosette and heading stages, respectively. The average annual Kc of Chinese cabbage was 0.81. Although Kc was higher in the middle and late periods, the water use efficiency was still 28.96 kg·ha−1·mm−1. The annual ET of Chinese cabbage was 505.3 mm. The study revealed the variation pattern of ET and Kc of Chinese cabbage, which provides an important scientific basis for the irrigation management of Chinese cabbage and is of great significance to guide the practice of water-saving vegetable planting. [ABSTRACT FROM AUTHOR]

As a traditional Chinese medicine, Ginkgo biloba leaves have been used in treatments on several diseases for many centuries. The constituents of Ginkgo biloba leaves such as flavonol glycosides and terpene trilactones are active substances associated with the treatment effect. There is not subspecies of Ginkgo biloba so that the constituents of the leaves are mainly related to the geographical origins. In order to trace the geographical origins of Ginkgo biloba , the LIBS technology can be used to measure the spectra of the Ginkgo biloba leaves. However, the spectra of Ginkgo biloba leaves from different origins are very similar so that it is hard to identify them. In this work, three multivariate statistical methods, PCA, LDA and SVM were used to analyze the spectra. The Ginkgo biloba leaves were collected from 8 different locations in Xi'an City, China. The spectra were pre-treated by PCA firstly. Then, the LDA and SVM were adopted to process the data furthermore. When the first 30 principal components (PCs) determined by PCA were used as new input variables, a very good recognition effect was gotten by both LDA and SVM models. The accuracies of origin identification can be up to 97.50% and 96.25% for LDA and SVM, respectively. The result demonstrates that LIBS technology can be used to trace the geographical origins of Ginkgo biloba accurately with the help of multivariate statistical methods. [Display omitted] • The constituents of the Ginkgo biloba leaves are mainly related to their geographical origins. In order to trace the geographical origins of Ginkgo biloba , the LIBS technology was used to measure the fresh Ginkgo biloba leaves directly. • In the most studies on the traceability of plants, the samples were products of industrial processing, and the spatial distances of the production were relatively large. In this work, we try to trace fresh raw materials collected in a small area. • This work demonstrates that LIBS can be used to identify the different fresh plants in terms of their geographical origin combined with multivariate statistical methods. [ABSTRACT FROM AUTHOR]

The large yellow croaker (Larimichthys crocea) is one of the most important marine economic fish in the southeast coast of China. However, hypoxia stress become a major obstacle to the benign development of L. crocea industry. To understand the energy metabolism mechanism adapted to hypoxia, we analyzed the transcriptome and physiology of L. crocea liver in response to hypoxia stress for different durations. We obtained 243,756,080 clean reads, of which 83.38% were successfully mapped to the reference genome of L. crocea. The heat map analysis showed that genes encoding enzymes involved in glycolysis/gluconeogenesis were significantly upregulated at various time points. Moreover, genes encoding enzymes related to the citrate cycle, oxidative phosphorylation, and amino acid metabolism were significantly downregulated at 6 and 24 h, but upregulated at 48 and 96 h. The change of liver in physiology processes, including respiratory metabolism, and activities of the carbohydrate metabolism enzymes showed a similar trend. The results revealed that the respiratory metabolism of L. crocea was mainly anaerobic within 24 h of hypoxia stress, and aerobic metabolism was dominant after 24 h. Carbohydrate metabolism plays a crucial role in energy supply and amino acid metabolism is an important supporting character to cope with acute hypoxia stress. There was no significant change in lipid utilization under short-term acute stress. This study increases our understanding of the energy metabolism mechanism of the hypoxia response in fish and provides a useful resource for L. crocea genetics and breeding. • The transcriptome of L. crocea liver was analyzed under hypoxia stress. • The adjustment of energy metabolism might be an effective strategy through which L. crocea responds to hypoxia stress. • Anaerobic metabolism and aerobic metabolism play an important role in the L. crocea 's response to hypoxia stress. • Glycometabolism plays a crucial role in hypoxia adaptation and amino acid metabolism is an important supporting character. [ABSTRACT FROM AUTHOR]

Larimichthys crocea is an economically important marine fish in China. To date, the molecular mechanisms underlying testicular development and spermatogenesis in L. crocea have not been thoroughly elucidated. In this study, we conducted a comparative transcriptome analysis between testes (TES) and pooled multiple tissues (PMT) (liver, spleen, heart, and kidney) from six male individuals. More than 54 million clean reads were yielded from TES and PMT libraries. After mapping to the draft genome of L. crocea, we acquired 25,787 genes from the transcriptome dataset. Expression analyses identified a total of 3853 differentially expressed genes (DEGs), including 2194 testes-biased genes (highly expressed in the TES) and 1659 somatic-biased genes (highly expressed in the PMT). The dataset was further annotated by blasting with multi-databases. Functional genes and enrichment pathways involved in spermatogenesis and testicular development were analyzed, such as the neuroactive ligand–receptor interaction pathway, gonadotropin-releasing hormone (GnRH) and mitogen-activated protein kinase (MAPK) signaling pathways, cell cycle pathway, and dynein, kinesin, myosin, actin, heat shock protein (hsp), synaptonemal complex protein 2 (sycp2), doublesex- and mab-3-related transcription factor 1 (dmrt1), spermatogenesis-associated genes (spata), DEAD-Box Helicases (ddx), tudor domain-containing protein (tdrd), and piwi genes. The candidate genes identified by this study lay the foundation for further studies into the molecular mechanisms underlying testicular development and spermatogenesis in L. crocea. [ABSTRACT FROM AUTHOR]

Background: There are many public health issues to resolve regarding rare diseases, including a lack of data from large-scale studies. The objective of this study was to explore fundamental data for a list of rare diseases in China, based on a hospitalization summary reports (HSRs) database. The Target Rare Diseases List (TRDL) 2017 was generated using an expert consensus method in which experts listed diseases according to research priorities. Using codes of the 10th revision of the International Statistical Classification of Diseases and Related Health Problems (ICD-10) and key search terms of rare diseases in English and Chinese, data were obtained from HSRs of 96 hospitals, covering a population of over 15 million in China from 2014 to 2015. We extracted and analyzed information on demographics, hospitalizations, and readmissions.Results: A total 281 rare diseases were included in the TRDL 2017. Altogether, 106,746 hospitalizations for a rare disease were captured from 1 January 2014 to 31 December 2015, accounting for 0.69% of inpatients during the same period. The top 10 rare diseases with most cases on the TRDL 2017 were thalassemia, idiopathic pulmonary arterial hypertension, pulmonary Langerhans cell histiocytosis, moyamoya disease, motor neuron disease, idiopathic pulmonary fibrosis, systemic sclerosis, hepatolenticular degeneration, coarctation of the aorta, and transposition of the great arteries. Among the 24 cities in the database, the five cities with the most types of the rare disease were Beijing, Changsha, Guangzhou, Shanghai, and Chengdu, with 191, 162, 143, 141, and 133 types, respectively. The five cities with most cases of the 281 rare diseases were Beijing, Guangzhou, Shanghai, Nanning, and Chengdu. The age distribution of rare diseases was 52% for the age group 25-64 years, and 27% of cases in the age group of 0-14 years were among children. The 10 highest readmission rates ranged from 35 to 65%.Conclusions: This study provided the TRDL 2017 and descriptive analysis of 281 rare diseases in a hospitalized population. Our study reveals important fundamental information that will be useful in national policy making and legislation; registry implementation; and diagnosis, treatment, and prevention of rare diseases in China. [ABSTRACT FROM AUTHOR]

Abstract: Retrofitting existing power plants for CO2 capture poses numerous constraints. The layout of the original process and the structure of the existing equipment cause various special problems in the design process as well as influence system performance. In view of these, this paper carries out process simulations, characteristic analysis, and system integration of CO2 capture based on an existing typical coal-fired power plant in China with supercritical parameters. The main constraints encountered in decarburized retrofitting of the existing power plants using monoethanolamine solution are analyzed. In addition, several special system integration schemes for CO2 capture in an existing 600 MW power generation unit are put forward. The results reveals that, due to the constraints in the layout of the original process and the structure of the existing equipment, efficiency penalty of CO2 capture in an existing power plant will be as high as 13.73%-points, higher than a newly redesigned power plant by 3.70%-points. And through special system integrations, the efficiency of such retrofitting existing power plant can increase by 4.15%-points. The research of this paper may provide feasible technology solutions for the decarburized retrofitting of existing power plants and promote CCS (CO2 capture and storage) technologies into application. [Copyright &y& Elsevier]

Background: A recent epidemic of melamine contamination of baby formula in China has been associated with the development of urinary tract stones, though the clinical manifestations and predisposing factors are incompletely delineated. Methods: We administered a questionnaire to the parents of children 36 months of age or younger who were being screened for a history of exposure to melamine and symptoms of, and possible predisposing factors for, urinary tract stones. In addition, we performed urinalysis, renal-function and liver-function tests, urinary tests for biochemical markers and the calcium:creatinine ratio, and ultrasonography. Powdered-milk infant formulas were classified as having a high melamine content (>500 ppm), a moderate melamine content (<150 ppm), or no melamine (0 ppm); no formulas contained between 150 and 500 ppm of melamine. Results: Contaminated formula was ingested by 421 of 589 children. Fifty had urinary stones, including 8 who had not received melamine-contaminated formula; 112 were suspected to have stones; and 427 had no stones. Among children with stones, 5.9% had hematuria and 2.9% had leukocyturia, percentages that did not differ significantly from those among children who were suspected to have stones or those who did not have stones. Serum creatinine, urea nitrogen, and alanine aminotransferase levels were normal in the 22 children with stones who were tested. Four of the 41 children (9.8%) who had stones and in whom urinary markers of glomerular function were measured had evidence of abnormalities; none had tubular dysfunction. Children exposed to high-melamine formula were 7.0 times as likely to have stones as those exposed to no-melamine formula. Preterm infants were 4.5 times as likely to have stones as term infants. Conclusions: Prematurity and exposure to melamine-contaminated formula were associated with urinary stones. Affected children lacked typical signs and symptoms of urolithiasis. N Engl J Med 2009;360:1067-74. [ABSTRACT FROM AUTHOR]

Numerous studies have shown that a wide range of pharmaceuticals are present in the environment and many of their adverse biological effects on the aquatic ecosystem and human health are unknown. Due to the high population density and large number of pharmaceuticals produced and consumed in China, a systematic approach is needed to identify pharmaceuticals that require greater attention. The present study provides a ranking of pharmaceuticals in China in terms of their occurrence (O), persistence, bioaccumulation, and toxicity (PBT) based on the predicted environmental concentration (PEC). The total and partial ranking method implemented in the decision analysis by ranking techniques (DART) tool was used, which is an easy-to-use tool for the analysis of datasets. Using the DART approach, 10 pharmaceuticals were selected as priority compounds. These pharmaceuticals included antibiotics, anti-inflammatory and antilipidemic. In order to identify the characteristics of the priority pharmaceuticals, ecotoxicological endpoints were considered. The results of this study and the priority list facilitate the selection of candidate pollutants in future monitoring studies. • Pharmaceutical ranking list was determined based on multi criteria. • The total and partial approach were applied for priority study. • Hazard characterization was identified with 10 pharmaceuticals for potential monitoring purpose. [ABSTRACT FROM AUTHOR]

Abstract Biochar application is proposed having a potential of inhibiting greenhouse gases emissions from paddy fields, which is considered to be a main source of atmospheric greenhouse gases. However, the impacts of biochar on greenhouse gases from paddy field have not been investigated under controlled irrigation (CI). Field experiments were conducted during 2016–2017 to determine the effect of biochar application combined with controlled irrigation on rice yield and methane (CH 4) and nitrous oxide (N 2 O) emission from paddy fields in the Taihu Lake Region of China. Four treatments (0 t ha−1 biochar +CI, 20 t ha−1 biochar +CI, 40 t ha−1 biochar +CI, and 40 t ha−1 biochar + flooding irrigation (FI), named CA, CB, CC and FC, respectively) were designed in this study. The results showed that the effect of biochar application on greenhouse gases emissions from paddy fields under controlled irrigation had significant interannual differences. In the first season, CC decreased the global warming potential(GWP) of CH 4 and N 2 O emission, and the CB increased the GWP of CH 4 and N 2 O emission compared to CA, but these differences were not significant. For the second season, CB and CC decreased the GWP of CH 4 and N 2 O emission by 35.7% and 21.5% significantly compared to CA due to the significant mitigation of CH 4 and N 2 O emission. Biochar application significantly increased CH 4 emission and decreased N 2 O emission from paddy fields under flooding irrigation compared to controlled irrigation (CC), which led to the FC's GWP was 1.70 and 5.47 times higher than CC's in the first and second season. In addition, biochar application increased soil organic carbon, dissolved organic carbon and total nitrogen contents of paddy fields under controlled irrigation. And CB and CC increased rice yield by 16.7% and 24.3% and irrigation water productivity by 26.1% and 30.8% compared with CA (mean of two seasons). These results suggest that 20 and 40 t ha−1 biochar can be utilized under controlled irrigation not only for mitigation of CH 4 and N 2 O emission but also to increase rice yield, soil fertility and irrigation water productivity. Therefore, the combination of biochar amendment and controlled irrigation might be a good option for mitigating greenhouse gases emission and realizing the sustainable utilization of soil and water resources of paddy fields in the Taihu Lake Region of China. Highlights • 20 t ha−1 biochar application significantly increased soil DOC, TN, NH 4 +-N and decreased NO 3 −-N concentrations. • Biochar amendments increased rice yield and irrigation water productivity (mean of two rice seasons). • Biochar application combined with controlled irrigation reduced the mean GWP of two rice seasons. [ABSTRACT FROM AUTHOR]

Abstract Background aims Cytokine-induced killer (CIK) cells are the most commonly used cellular immunotherapy for multiple tumors. To further confirm whether chemotherapy with CIK cells improves clinical effectiveness and to reveal its optimal use in non–small cell lung cancer (NSCLC), we systematically reevaluated all relevant studies. Methods We collected all studies about chemotherapy with CIK cells for NSCLC from the Medline, Embase, Web of Science, China National Knowledge Infrastructure Database (CNKI), Chinese Scientific Journals Full-Text Database (VIP), Wanfang Data, China Biological Medicine Database (CBM), Cochrane Central Register of Controlled Trials (CENTRAL), Chinese clinical trial registry (Chi-CTR), World Health Organization (WHO) International Clinical Trials Registry Platform (ICTRP) and U.S. clinical trials. We evaluated their quality according to the Cochrane evaluation handbook of randomized controlled trials (RCTs) (version 5.1.0), extracted the data using a standard data extraction form, synthesized the data using meta-analysis and finally rated the evidence quality using the Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach. Results Thirty-two RCTs with 2250 patients were included, and most trials had unclear risk of bias. The merged risk ratios values and their 95% confidence intervals of meta-analysis for objective response rate, disease control rate, 1- and 2-year overall survival rates, 1- and 2-year progression-free survival rates were as following: 1.45 (1.31–1.61), 1.26 (1.16–.37), 1.42 (1.23–1.63), 2.06 (1.36–3.12), 1.93 (1.38–2.69) and 3.30 (1.13–9.67). Compared with chemotherapy alone, all differences were statistically signif i cant. CIK cells could increase the CD3+ T cells, CD3+ CD4+ T cells, NK cells and the ratio of CD4+/CD8+ T cells. The chemotherapy with CIK cells had a lower risk of hematotoxicity, gastrointestinal toxicity, liver injury and a higher fever than that of chemotherapy alone. The evidence quality was "moderate" to "very low." Conclusions The available moderate evidences indicate that chemotherapy with CIK cells, especially autologous CIK cells, can significantly improve the tumor responses, 1- and 2-year overall and progression-free survival rates in patients with advanced NSCLC. This treatment does have a high risk of fever. The optimal use may be treatment with one or two cycles and in combination with vinorelbine and cisplatin, paclitaxel and cisplatin, or docetaxel and cisplatin. [ABSTRACT FROM AUTHOR]

A simple and effective method based on magnetic separation has been developed for the extraction of organochlorine pesticides (OCPs) from river water samples using magnetoliposomes as adsorbents. This method avoided the time-consuming column passing process of loading large volume samples in traditional SPE through the rapid isolation of magnetoliposomes with an adscititious magnet. Lipid bilayers formed on the surface of Fe 3 O 4 showed great adsorptive tendency towards analytes through hydrophobic interactions, and zwitterions headgroups endowed the outer surface of magnetoliposomes with hydrophilicity to improve the dispersing property of adsorbents in the sample matrix. The target analytes were detected by gas chromatography-tandem mass spectrometry, and the limits of detection obtained are in the range of 0.04–0.35 ng L − 1 . The relative standard deviations of intra- and inter-day are in the range of 2–5% and 4–7%, respectively. The proposed method was employed for analysis of six OCPs in the surface water samples from two cities along the Songhua River in different seasons. Compared with the traditional methods, the proposed method could reduce the consumption of the organic solvent and shorten the sample preparation time. [ABSTRACT FROM AUTHOR]

A letter to the editor is presented about the status of clinical research in China.

With the acceleration of urbanization, the proportion of surface imperviousness is increasing continuously in cities, resulting in frequent waterlogging disasters. In this context, storm water management, based on the low-impact development (LID) concept, offers an effective measure for the management of urban storm waters. First, the storm water management model (SWMM) was built for a typical cold climate city (Changchun) in China. Next, the two-stage calibrated model was employed to explore the surface runoff and storm sewer control effects of four LID combination plans. Finally, these plans were put through a "cost-benefit" evaluation through an analytic hierarchy process. According to the results, after using four LID plans, the reduction rates of peak runoff exceeded 40% and the problem of overflow load of the storm sewage was significantly mitigated. The infiltration-oriented Plan I proved to be the optimal plan, with the lowest proportions of the overflow nodes and full-load pipe sections in each return period, as well as with maximum overall performance. This study offers technical and conformed methodological support to cold cities for the prevention and control of waterlogging disasters and recycling of rainwater resources. • The low impact development (LID) combination measures of cold city are screened. • The two-stage calibration of the model has a higher accuracy. • Environmental and economic benefits of various LID plans were quantified. • Infiltration-oriented combination plan showed the maximum benefit. • Proposed evaluation system can inform the optimal selection of LID practices. [ABSTRACT FROM AUTHOR]

Purpose: Secukinumab, a monoclonal antibody targeting interleukin (IL)-17A, is approved for the treatment of psoriasis, psoriatic arthritis, ankylosing spondylitis, non-radiographic axial spondyloarthritis, enthesitis-related arthritis, and hidradenitis suppurativa. This study compared the pharmacokinetics (PK), safety, and immunogenicity of CMAB015, a candidate secukinumab biosimilar, with the reference product secukinumab (Cosentyx ® ) in healthy Chinese male subjects., Patients and Methods: This double-blind, parallel-group study randomized healthy Chinese male subjects (N=130) to receive either a single dose of 150 mg CMAB015 or secukinumab subcutaneously. Primary study endpoints were PK parameters such as the maximum concentration (C max ) and area under the curve from zero to infinity (AUC 0-inf ), while safety and immunogenicity were secondary endpoints., Results: The 90% confidence intervals (CIs) of the geometric mean ratios (GMRs) of C max and AUC 0-inf for CMAB015 to secukinumab were all within the bioequivalence limits (80.00-125.00%). Other PK parameters were comparable between the groups. The safety profile of CMAB015 was similar to that of secukinumab, with no serious adverse events related to treatment. The incidence of TEAEs was slightly higher in the CMAB015 group, but these events were mild to moderate in severity and did not lead to any withdrawals from the study. Immunogenicity analysis revealed low rates of anti-drug antibody (ADA) positivity, with similar rates between CMAB015 and secukinumab., Conclusion: This study demonstrated equivalent PK, comparable safety, and immunogenicity of CMAB015 to secukinumab in healthy Chinese male subjects. These findings support further clinical evaluation of CMAB015 as a secukinumab biosimilar., Trial Registration: The trial was registered on Clinicaltrials.gov (Identifier No. NCT05734482) and Chinadrugtrials.org.cn (Identifier No. CTR20230105)., Competing Interests: Chenguang Wang, Tianshu Yang, Xunmin Zhang, Yong Shan, Sheng Hou, and Hao Wang are affiliated with Taizhou Mabtech Pharmaceutical Co. Ltd. The authors report no other conflicts of interest in this work., (© 2024 Yao et al.)

This study aimed to investigate the knowledge about, attitudes toward, and acceptance and predictors of receiving the mpox vaccine among Chinese cancer patients. Patients were selected using a convenience sampling method. A web-based self-report questionnaire was developed to assess cancer patients' knowledge, attitudes, and acceptance regarding the mpox vaccine. Multivariate logistic regression analysis was used to determine predictors of acceptance of the mpox vaccine. A total of 805 cancer patients were included in this study, with a vaccine hesitancy rate of 27.08%. Approximately 66% of the patients' information about mpox and the vaccine came from the mass media, and there was a significant bias in the hesitant group's knowledge about mpox and the vaccine. Multivariable logistic regression analysis suggested that retirement; chemotherapy; the belief that the mpox vaccine could prevent disease, that vaccination should be compulsory when appropriate and that the mpox vaccine prevents mpox and reduces complications; the willingness to pay for the mpox vaccine; the willingness to recommend that friends and family receive the mpox vaccine; and the belief that the mpox vaccine should be distributed fairly and equitably were factors that promoted vaccination. The belief that mpox worsens tumor prognosis was a driving factor for vaccine hesitancy. This study investigated the knowledge of cancer patients about mpox and the vaccine, evaluated the acceptance and hesitancy rates of the mpox vaccine and examined the predictors of vaccination intention. We suggest that the government scientifically promote the vaccine and develop policies such as free vaccination and personalized vaccination to increase the awareness and acceptance rate of the mpox vaccine.

Objective: To investigate efficacy and prognostic factors in the treatment of adult newly-diagnosed acute myeloid leukemia (AML) with or without allogeneic hematopoietic stem cell transplantation (Allo-HSCT)., Methods: We retrospectively analyzed 668 patients with newly-diagnosed AML (non-M3 type) in the Department of Hematology at Shanghai Changhai Hospital from January 2012 to December 2021. Based on different induction chemotherapy regimens, patients were categorized into an IA (idarubicin, IDA + cytarabine, Ara-C) (3 + 7, regimen) group (n = 303) and a DA (daunorubicin, DNR + cytarabine, Ara-C) (3 + 7, regimen) group (n = 365) with or without allo-HSCT. Minimal residual disease (MRD), complete response (CR), overall response rate (ORR), progression-free survival (PFS), overall survival (OS), and adverse effects (AE) were analyzed and compared. Characteristics significantly associated with overall or progression-free survival (OS or PFS) upon univariate analysis were subsequently included in a Cox proportional hazard model., Results: This study used data from 668 AML patients. After induction therapy, the CR rate in the IA group was 70.63% and ORR was 79.87%, which were significantly higher than those in the DA group (with a CR rate of 56.99% and an ORR of 70.14%) ( P  = 0.0002 and P  = 0.0035, respectively). There were no significant differences in drug safety between the two chemotherapy regimens used in IA and DA ( P  > 0.05). The recurrence rate was lower in patients with an MRD < 0.001 than in patients with an MRD ≥ 0.001. A continuous negative MRD during the period is significant because it is associated with prolonged OS and PFS of AML patients. Data from 100 patients in the two groups who underwent allo-HSCT were analyzed using univariate analysis and the Cox proportional hazards model. From the multivariate analysis, MRD was found to be the only independent predictor of OS ( P  = 0.042; HR 1; 95%CI 0.00-0.76)., Conclusion: In the treatment of adult AML patients, IA regimen is associated with a high CR rate and ORR rate and does not increase treatment-related toxicity. IA regimen prolongs OS and PFS in AML patients and reduces the likelihood of leukemia cells' subsequent infiltration into the central nervous system. There is a high correlation between the level of MRD after treatment and the patient's bone marrow recurrence. To obtain superior treatment effects for patients undergoing allo-HSCT, the MRD should be reduced to less than 0.001 before pretreatment. A negative MRD before allo-HSCT can prolong OS in patients with AML. We examined the clinical characteristics and outcomes of AML patients in China, finding novel information on prognostic factors and primary treatment of AML that may be applicable in routine clinical practice.

Objectives: To establish age estimation models of northern Chinese Han adults using cranial suture images obtained by CT and multiplanar reformation (MPR), and to explore the applicability of cranial suture closure rule in age estimation of northern Chinese Han population., Methods: The head CT samples of 132 northern Chinese Han adults aged 29-80 years were retrospectively collected. Volume reconstruction (VR) and MPR were performed on the skull, and 160 cranial suture tomography images were generated for each sample. Then the MPR images of cranial sutures were scored according to the closure grading criteria, and the mean closure grades of sagittal suture, coronal sutures (both left and right) and lambdoid sutures (both left and right) were calculated respectively. Finally taking the above grades as independent variables, the linear regression model and four machine learning models for age estimation (gradient boosting regression, support vector regression, decision tree regression and Bayesian ridge regression) were established for northern Chinese Han adults age estimation. The accuracy of each model was evaluated., Results: Each cranial suture closure grade was positively correlated with age and the correlation of sagittal suture was the highest. All four machine learning models had higher age estimation accuracy than linear regression model. The support vector regression model had the highest accuracy among the machine learning models with a mean absolute error of 9.542 years., Conclusions: The combination of skull CT-MPR and machine learning model can be used for age estimation in northern Chinese Han adults, but it is still necessary to combine with other adult age estimation indicators in forensic practice.

The mortality rates for patients undergoing hemodialysis (HD) remain unacceptably high compared to the general population, and more specific information about the causes of death is not known. The study aimed to develop and validate a risk prediction model that uses common clinical factors to predict the probability of cardiovascular events in maintenance hemodialysis (MHD) patients. The study involved 3488 adult patients who received regular scheduled hemodialysis treatment at 20 hemodialysis centers in southwest China between June 2015 and August 2020, with follow-up until August 2021. The optimal parameter set was identified by multivariable Cox regression analyses and Cross-LASSO regression analyses and was used to establish a nomogram for predicting the risk of cardiovascular events in maintenance hemodialysis patients at 3 and 5 years. The performance of the model was evaluated using the consistency index (Harrell's C-index), the area under the receiver operating characteristic (ROC) curve, and calibration plots. The model was validated by tenfold cross-validation and bootstrapping with 1000 resamples. In the derivation cohort, the model yields an AUC of 0.764 [95% confidence interval (CI), 0.737-0.790] and 0.793 [CI, 0.757-0.829] for predicting the risk of cardiovascular events of MHD patients at 3 and 5 years. In the internal validation cohort AUC of 0.803 [95% CI, 0.756-0.849], AUC of 0.766 [95% CI, 0.686-0.846], and the external validation cohort AUC of 0.826 [95% CI, 0.765-0.888], AUC of 0.817 [95% CI, 0.745-0.889] at 3 and 5 years. The model's calibration curve is close to the ideal diagonal. By tenfold cross-validation analyses, the 3- and 5-year risk of cardiovascular events (AUC 0.732 and 0.771, respectively). By the bootstrap resampling method, the derivation cohort and validation cohort (Harrell's C-index 0.695 and 0.667, respectively) showed good uniformity with the model. The constructed model accurately predicted cardiovascular events of MHD patients in the 3rd and 5th years after dialysis. And the further research is needed to determine whether use of the risk prediction tool improves clinical outcomes., (© 2024. The Author(s).)

Background: SARS-CoV-2 strains have been of great concern due to their high infectivity and antibody evasion., Methods: In this study, data were collected on indigenous aggregated outbreaks in Nanjing from January 2020 to December 2022, caused by five strains including the original strain, the Delta variant, and the Omicron variant (BA.2, BA.5.2, and BF.7). The basic epidemiological characteristics of infected individuals were described and then parametric analysis of transmission dynamics was performed, including the calculation of incubation period, serial interval (SI), the basic reproductive number (R 0 ), and the household secondary attack rate (HSAR). Finally, we compared the trends of transmission dynamic parameters of different strains., Results: The incubation period for the original strain, the Delta variant, Omicron BA.2, Omicron BA.5.2, and Omicron BF.7 were 6 d (95% CI: 3.5-7.5 d), 5 d (95% CI: 4.0-6.0 d), 3 d (95% CI: 3.0-4.0 d), 3 d (95% CI: 3.0-3.0 d), and 2 d (95% CI: 2.0-3.0 d), respectively; Also, the SI of the five strains were 5.69 d, 4.79 d, 2.7 d, 2.12 d, and 2.43 d, respectively. Notably, the incubation period and SI of the five had both a progressive shortening trend ( p  < 0.001); Moreover, R 0 of the five were 2.39 (95% CI: 1.30-4.29), 3.73 (95% CI: 2.66-5.15), 5.28 (95% CI: 3.52-8.10), 5.54 (95% CI: 2.69-11.17), 7.39 (95% CI: 2.97-18.76), with an increasing trend gradually ( p  < 0.01); HSAR of the five were 25.5% (95% CI: 20.1-31.7%), 27.4% (95% CI: 22.0-33.4%), 42.9% (95% CI: 34.3-51.8%), 53.1% (95% CI: 45.0-60.9%), 41.4% (95% CI, 25.5-59.3%), also with an increasing trend ( p  < 0.001)., Conclusion: Compared to the original strain, the incubation period and SI decreased while R 0 and HSAR increased, suggesting that transmission in the population was faster and the scope of the population was wider. Overall, it's crucial to keep implementing comprehensive measures like monitoring and alert systems, herd immunization plans, and outbreak control., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Ma, Chen, Wang, Wang, Zhao, Zhu, Yan, Ding and Ding.)

To investigate the dynamical transmission behavior of pollutants and explore the roles played by monitoring stations in regional air pollutants transportation, we constructed a new model for the dynamical transmission index by adopting a statistics model that employs complex network analysis along with terrain data, meteorological variables, and air quality data. The study is conducted in Beijing-Tianjin-Hebei region with 70 stations in 13 cities. The findings indicated that the regional dynamical transmission networks were characterized by the participation of 67 out of 70 stations, as determined by node number. Among the model characteristics, the average path length and the average clustering coefficient, within the ranges of 2.08-2.32 and 0.26-0.51, respectively, maintained reasonable small-world characteristic. For the seasonal transmission features, the networks for PM 2.5 , PM 10 in winter, and O 3 in summer shared similar modeling characteristics with those of yearly networks. This suggested that the networks for these two seasons could represent the yearly transmission features. By employing the entropy weight method, the key monitoring stations numbered 1011 A, 1026 A, and 1010 A, which are located in Tianjin, Shijiazhuang, and Beijing, exerted significant impacts on air pollution transmission path in cities. The novel model has demonstrated its soundness and effectiveness in terms of capturing the behavior of transmission as well as the distinguishing roles of these crucial monitoring stations. This methodology could be employed for the construction of additional monitoring stations, identification of possible pollution sources, and prioritization of key pollution areas, thus providing valuable insights for environmental protection and management., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Inc.)

Objectives: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lineage B.1.617.2 (also named the Delta variant) was declared as a variant of concern by the World Health Organization (WHO). This study aimed to describe the outbreak that occurred in Nanjing city triggered by the Delta variant through the epidemiological parameters and to understand the evolving epidemiology of the Delta variant., Methods: We collected the data of all COVID-19 cases during the outbreak from 20 July 2021 to 24 August 2021 and estimated the distribution of serial interval, basic and time-dependent reproduction numbers (R 0 and R t ), and household secondary attack rate (SAR). We also analyzed the cycle threshold (Ct) values of infections., Results: A total of 235 cases have been confirmed. The mean value of serial interval was estimated to be 4.79 days with the Weibull distribution. The R 0 was 3.73 [95% confidence interval (CI), 2.66-5.15] as estimated by the exponential growth (EG) method. The R t decreased from 4.36 on 20 July 2021 to below 1 on 1 August 2021 as estimated by the Bayesian approach. We estimated the household SAR as 27.35% (95% CI, 22.04-33.39%), and the median Ct value of open reading frame 1ab (ORF1ab) genes and nucleocapsid protein (N) genes as 25.25 [interquartile range (IQR), 20.53-29.50] and 23.85 (IQR, 18.70-28.70), respectively., Conclusions: The Delta variant is more aggressive and transmissible than the original virus types, so continuous non-pharmaceutical interventions are still needed., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Wang, Ma, Ding, Xu, Zhang, Zhang, Dai, Tao, Wang, Cheng, He, Du, Feng, Yang, Wang, Xie, Xu, Liu, Chen, Li, Wu, Ye, Yang, Fan, Zhou and Ding.)

Purpose: Congenital bilateral absence of the vas deferens (CBAVD) is a major cause of obstructive azoospermia and male factor infertility. CBAVD is mainly caused by mutations in the genes encoding CFTR (cystic fibrosis transmembrane conductance regulator) and ADGRG2 (adhesion G protein-coupled receptor G2). This study aimed to describe CFTR and ADGRG2 variations in 46 Chinese CBAVD patients and evaluated sperm retrieval and assisted reproductive technology outcomes., Methods: The CFTR and ADGRG2 genes were sequenced and analyzed by whole-exome sequencing (WES), and variations were identified by Sanger sequencing. Bioinformatic analysis was performed. We retrospectively reviewed the outcomes of patients undergoing sperm retrieval surgery and intracytoplasmic sperm injection (ICSI)., Results: In total, 35 of 46 (76.09%) patients carried at least one variation in CFTR, but no copy number variants or ADGRG2 variations were found. In addition to the IVS9-5 T allele, there were 27 CFTR variations, of which 4 variations were novel and predicted to be damaging by bioinformatics. Spermatozoa were successfully retrachieved in 46 patients, and 39 of the patients had their own offspring through ICSI., Conclusion: There are no obvious hotspot CFTR mutations in Chinese CBAVD patients besides the IVS9-5 T allele. Therefore, WES might be the best detection method, and genetic counseling should be different from that provided to Caucasian populations. After proper counseling, all patients can undergo sperm retrieval from their epididymis or testis, and most of them can have their own children through ICSI., (© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

The interactive effects of both biochar (BC) and electrochemistry (EC) can affect nitrogen (N) removal process. However, little is known about how this function in constructed wetland (CW) systems. In this study, an electrochemically (EC) coupled BC-amended saturated subsurface vertical flow constructed wetland (BECW) systems were established to enhance nitrogen (N) removal. Other three CW systems: without BC and EC (CW); with EC only (ECW); and with BC only (BCW) were performed as controls. Results indicated that the total nitrogen (59.88%-93.03%) and nitrate‑nitrogen (83.14%-100%) of the BECW system were significantly enhanced (p < 0.05) compared with the control systems. Treated WWTP tail-water could meet Class-IV of the Surface Water Quality Standard (GB3838-2002) in China by the BECW system. The enhanced N removal in the BECW system could be attributed to (1) the autotrophic denitrification process in which H 2 and Fe 2+ provided by the cathode and anode acted as electron donors; and (2) BC addition acting as substrate could improve the activity, diversity and richness of microorganisms. Microbial community analysis further indicated that high N removal in the BECW system was significantly dependent on the synergy between the heterotrophic and autotrophic denitrifiers, facilitated by BC and EC interaction. Results illustrate that the BECW system is a feasible and eco-sustainable technology for treating low C/N tail-water from WWTPs. This work provides a novel and fundamental understanding of the electrochemically coupled biochar-amended CW system. These results could serve as a theoretical basis for the engineered applications in the deep purification of WWTPs' tail-water., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Background: To describe the virologic and immunologic outcomes among people living with HIV (PLHIV) coinfected with SARS-CoV-2., Setting: Wuhan, China., Methods: Thirty-five coinfected patients were identified by matching the reported cases in National Notifiable Infectious Disease Report system for COVID-19 and HIV in Wuhan by time of April 19, 2020. Questionnaire-based survey and follow-up with blood sample collection were used to obtain characteristics before COVID-19 and after recovery. Nonparametric Mann-Whitney U test, χ2, or Fisher exact test, Mcnemar test, and Wilcoxon test were conducted., Results: Twenty of the 35 coinfected patients were identified as asymptomatic/mild/moderate COVID-19 (nonsevere group) and 15 were identified as severe/critical (severe group). The severe and nonsevere group had no differences in demographics, HIV baseline status, the intervals between last tests and follow-up tests for CD4+ cell count and HIV-1 viral load (all P > 0.05). Overall, there was a significantly increased number of coinfected patients with HIV-1 viral load ≥20 copies/mL after recovery (P = 0.008). The median viral load increased significantly after recovery in severe group (P = 0.034), whereas no significant change of HIV-1 viral load was observed in the nonsevere group. Limited change of CD4+ cell count was found (all P > 0.05)., Conclusion: The coinfection of SARS-CoV-2 may put PLHIV at greater risk for HIV-1 viral rebound especially for severe/critical COVID-19, whereas it had limited impacts on CD4+ cell count. Whether continuous antiretroviral therapy against HIV infection would have significant impacts on CD4+ cell count among PLHIV coinfected with SARS-CoV-2 needs further research., Competing Interests: The authors have no funding or conflicts of interest to disclose., (Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.)

Background: Thromboembolism is a life-threatening, limb-threatening or organ-threatening complication that occurs in patients with primary nephrotic syndrome (NS). There are few studies on the spectrum, complications and outcomes of thrombosis in children with NS. This study aimed to determine the spectrum of thrombosis and its relationship with the nephrotic state, treatment and outcomes in children and adolescents with primary NS., Methods: The medical records of subjects aged 1-18 years with NS complicated with thromboembolism treated at our centre within the last 26 years were retrieved. Data on the status of NS, site, symptoms and signs, laboratory investigations, diagnosis, treatment, complications and outcomes of thrombosis were collected and reviewed retrospectively. A severe complication was defined as a condition associated with thrombosis requiring a special diagnostic modality to confirm or a specific treatment such as surgical intervention. The outcome of thrombosis was defined as the status of thrombosis, as determined by imaging methods and the functional status with respect to the anatomic sites of thrombosis at the last follow-up. The permanent dysfunction of an organ or limb related to thrombosis was defined as a sequela., Results: We observed thrombosis in 1.4% (27/1995) of subjects with NS during the study period. There were 27 subjects with thrombosis, including 21 males and 6 females. Thrombosis was observed in 51.9% (14/27) of the study participants with steroid resistant NS. Most episodes of thrombosis occurred during the active stage of NS; however, 7.4% of thrombosis cases occurred during the remission of proteinuria. Renal vein thrombosis (33.3%) and pulmonary embolism (25.9%) were the most common types of thrombosis. Among the 17 subjects biopsied, minimal change disease and membranous nephropathy were the two most common findings. Six (22.2%) subjects experienced severe complications or sequelae; 1 had persistent intracranial hypertension, 1 had intestinal perforation, 1 had hypoxemia and pulmonary hypertension, 1 had lameness, 1 had epilepsy, and 1 had an askew mouth due to facial paralysis. In 19 (70.4%) subjects, the symptoms resolved completely or improved without severe complications or sequelae., Conclusions: Thrombosis mostly occurred in males of school age during the active stage of NS. Renal vein thrombosis and pulmonary embolism were the most common types of thrombosis. In most patients with thrombosis, the symptoms improved completely without complications with standard anticoagulation therapy. However, 22.2% had severe complications or sequelae requiring an advanced diagnostic modality and aggressive treatment.

Plots were set on the top, middle and bottom of the stony desert alluvial fan in the middle of southern foothills of the Tianshan Mountains. With plant height and crown size as indicators, the spatial heterogeneity of Ephedra przewalskii populations in the stony desert were studied using geostatistical methods. Understanding the spatial heterogeneity characteristics of E. przewalskii populations could provide scientific basis for vegetation protection and ecological restoration in stony deserts. The results showed that E. przewalskii had a patchy distribution at the top of the alluvial fan. At the middle and bottom of the alluvial fan, it showed a banded distribution. The band width in the middle was larger than that at the bottom. From the top to the bottom of the alluvial fan, the overall plant height and crown size of E. przewalskii populations decreased first and then increased. The average plant height on the top, middle and bottom of the alluvial fan was 40.34, 21.07, 36.96 cm, and the crown size were 1.09, 0.80, 1.43 m 2 , respectively. The best fitting models for plant height of E. przewalskii were the exponential model, the exponential model, and the linear model at the top, middle and the bottom of the alluvial fan, respectively, while the best fitting models for crown size were exponential model, spherical model, and linear model. From the top to the bottom of the alluvial fan, the fractal dimension value of plant height and crown size of E. przewalskii ranged from 1.909 to 1.889, indicating that the spatial pattern of E. przewalskii populations was simple and the spatial homogeneity was high. From the top to the bottom of the alluvial fan, the spatial distance of the anisotropy of plant height and crown size of E. przewalskii gradually shortened. At the top, middle and bottom of the alluvial fan, the spatial distances where the plant height and crown showed anisotropy were >60 m, 42-46 m and 23-27 m, respectively.

Biochar has been widely applied to paddy fields to improve soil fertility, crop productivity and carbon sequestration, thereby leading to variations in the CO 2 exchange between the paddy fields under flooding irrigation and the atmosphere, as indicated by many previous reports. However, few relevant reports have focused on paddy fields under water-saving irrigation. This study conducted a field experiment to investigate the effects of three biochar addition rates (0, 20 and 40 t ha -1 ) on the CO 2 exchange between paddy fields under controlled irrigation (CI, a water-saving irrigation technique) and the atmosphere in the Taihu Lake region of Southeast China. Our results showed that biochar addition increased the paddy field ecosystem respiration (R eco ) and the soil respiration rate (R s ) in the CI paddy fields. And biochar application increased the total CO 2 emissions and the total soil CO 2 emissions, especially at a rate of 40 t ha -1 . In contrast, gross primary productivity (GPP) was decreased and the net ecosystem exchange of CO 2 (NEE) was increased with biochar addition. However, biochar addition at a rate of 20 t ha -1 significantly increased the total CO 2 absorption and the net CO 2 absorption of the CI paddy fields (p < 0.05), whereas biochar addition at a rate of 40 t ha -1 had no effect on the total CO 2 absorption and decreased the total net CO 2 absorption. At the same time, biochar addition significantly increased soil catalase, invertase and urease activities and contributed substantially to the increase in soil invertase activity. In addition, the soil bacterial, fungal and actinomycetal abundances were evidently increased with biochar addition, of which the soil fungal abundance showed the greatest increase. A high correlation was observed between soil catalase and invertase activities and soil microbial abundance. R eco was highly correlated with air and soil temperatures and soil enzyme activity. A significant quadratic polynomial correlation was observed between GPP and leaf area index (p < 0.01). The combination of biochar addition at a rate of 20 t ha -1 and water-saving irrigation has the potential to increase the size of the carbon sink and promote soil enzyme and microbial activities in paddy field ecosystems., (Copyright © 2020 Elsevier Ltd. All rights reserved.)