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Background and objective Isoliquiritigenin (ISL) is an important pharmacological constituent of Glycyrrhiza glabra, which possesses a range of physiological and pharmacological activities, as well as significant antitumor activity, and can be used as a potential drug for targeted cancer therapy. LINC01503 is an oncogene, which has been closely associated with the malignant biological processes of many cancers. The aim of this study was to investigate the effects of ISL on the proliferation, apoptosis, invasion and migration of lung squamous carcinoma cells by regulating LINC01503. Methods Plasma was collected from lung squamous carcinoma patients and healthy individuals treated at Tangshan People's Hospital from January 2021 to December 2022. The expression of LINC01503 in lung squamous carcinoma plasma, tissues and cells was detected by real-time quantitative fluorescence polymerase chain reaction (qRT-PCR). Lung squamous carcinoma cells were treated with different concentrations of ISL for 24 h, and LINC01503 expression was detected by qRT-PCR. The cells were treated in groups: si-NC group, si-LINC01503 group, DMSO (0.1% dimethyl sulfone) group, ISL group, pc DNA3.1(+)-NC group, pc DNA3.1(+)-LINC01503 group, ISL+pc DNA3.1(+)-NC group and ISL+pc DNA3.1(+)- LINC01503 groups. CCK-8 assay, clone formation assay, flow cytometry, Transwell assay and scratch assay were used to explore the effect of LINC01503 on the functional phenotype of lung squamous carcinoma cells. Results Fluorescence in situ hybridization results showed that the average fluorescence intensity of LINC01503 in tissue microarrays of lung squamous carcinoma patients was higher than that in paracancerous tissues (P<0.05). The expression of LINC01503 in the plasma of patients with lung squamous carcinoma was higher than that in the plasma of healthy individuals (P<0.05). Knockdown of LINC01503 inhibited the proliferation, invasion and migration of lung squamous carcinoma cells and promoted apoptosis (P<0.05). ISL inhibited the proliferation, invasion, migration and promoted apoptosis of lung squamous carcinoma cells (P<0.05). Overexpression of LINC01503 followed by intervention with ISL reversed the promotional effect of overexpression of LINC01503 on the proliferation, invasion and migration of lung squamous carcinoma cells as well as the inhibitory effect on apoptosis (P<0.05). Conclusion LINC01503 was highly expressed in lung squamous carcinoma, and LINC01503 could promote the proliferation, invasion and migration of lung squamous carcinoma cells and inhibit the apoptosis, ISL could inhibit the proliferation, invasion and migration of lung squamous carcinoma cells and promote apoptosis of lung squamous carcinoma cells by regulating the expression of LINC01503. [ABSTRACT FROM AUTHOR]

Introduction: The Vel– phenotype is a rare blood group, and it is challenging for identifying this phenotype due to limited available reagents. Moreover, there are relatively few studies on genomic editing of erythroid antigens and generation of knockout (KO) cell lines at present. Methods: To identify the high-efficiency small-guiding RNA (sgRNA) sequence, candidate sgRNAs were transfected into HEK 293T cells and analyzed using Sanger sequencing. Following this, the high-efficiency sgRNA was transfected into K562 cells using lentivirus transduction to generate KO Vel blood group gene cells. The expression of the Vel protein was detected using Western blot on single-cell clones. Additionally, flow cytometry was used to detect the erythroid markers CD235a and CD71. Hemoglobin quantification and Giemsa staining were also performed to evaluate the erythroid differentiation of KO clones induced by hemin. Results: The high-efficiency sgRNA was successfully obtained and used for CRISPR-Cas9 editing in K562 cells. After limiting dilution and screening, two KO clones had either deleted 2 or 4 bases and showed no expression of the Vel protein. In the hemin-induced KO clone, there was a significant difference in erythroid marker and hemoglobin quantification compared to untreated cells. The morphological changes were also observed for the hemin-induced KO clone. Conclusion: In this study, a highly efficient sgRNA was screened out and used to generate Vel erythroid antigen KO single-cell clones in K562 cells. The edited cells could then be induced to undergo erythroid differentiation with the use of hemin. [ABSTRACT FROM AUTHOR]

To qualitatively and quantitatively distinguish the boundaries of commercial strong-, mellow- and light- fragrant rapeseed oils in Chinese market, 24 commercial rapeseed oils were symmetrically analyzed to characterize their profiles of physical/chemical components, key aroma compounds, and nutrients and their concentrations. Results showed that strong- fragrant flavor rapeseed oil had highest AV of 0.25–1.73 mg/g, PV of 0.50–3.37 mmol/kg, Lovibond red reading of > 2.0, total tocopherol of > 505 mg/kg, total sterols of over 4445 mg/kg, and average CoQ10 contents of > 204 mg/kg, followed by mellow- and light- fragrant flavor rapeseed oils. Strong- fragrant rapeseed oil held the highest roasted odor and nutty flavor, while mellow- fragrant rapeseed oil had more acceptable taste with highest soft and spicy taste values. The typical volatile compounds such as hexanal, dimethyl sulfoxide and 5-hexenenitrile could be used to quantitatively and qualitatively distinguish three types of rapeseed oils using PCA Cluster analysis. These findings would provide a reference to multi-dimensionally distinguish and select the favorable rapeseed oil for consumers from these types of commercial fragrant rapeseed oils. [ABSTRACT FROM AUTHOR]

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Detecting sulfoxide in sediment and water is an essential channel in understanding redox processes and maintaining water quality. Therefore, the creation of quick and efficient sulfoxide detection techniques is crucial to comprehending the ecotoxicity of sulfoxides. However, sulfur oxides were mainly detected in a pure water system, which greatly limited their potential practical applications. Difficulties related to selecting a detection system are a common bottleneck for applications. Herein, a brand-new fluorescent probe called BZ-QL was designed. This probe emits different fluorescence intensities in the sediment and water samples from a natural lake (China's Dianchi Lake). For the first time, the detection of sulfoxide in natural lake was realized on the basis of the correlation of the gene abundance of sulfate-reducing bacteria (SRB) with fluorescence intensities. Results indicated a significant negative correlation between the fluorescence intensities of BZ-QL and the abundance of dsrA for the water samples (R 2 = 0.35, P < 0.01) and sediments (R 2 = 0.16, P < 0.05). The combination of fluorescence intensities and quantitative polymerase chain reaction (qPCR) of SRB could provide unique techniques for detecting sulfur oxides in water samples. Compared with other sulfur dioxide fluorescent probes, the fluorescence detection method in this study introduces the parameter of gene abundance, and applies the probe technology to detect sulfur oxides in natural lakes. In the future, this simple and rapid detection technology could be converted into kits for promotion. In addition, other compounds could be detected by changing the recognition site of the probe. [Display omitted] • BZ-QL could emit different fluorescent signals in the water body and sediments. • Realized the detection of sulfoxide in natural lake based on a unique technology. • A novel tool for the direct detection of sulfur dioxide will be established. • The fluorescence and qPCR will provide a new way for detection of sulfur dioxide. [ABSTRACT FROM AUTHOR]

Abstract: Ultrasoft X-rays have been shown to be very efficient in inducing chromosomal aberrations in mammalian cells. The present study was aimed to evaluate the modifying effects of DMSO (a potent scavenger of free radicals) on the frequencies of chromosome aberrations induced by soft X-rays. Confluent held G1 Chinese hamster cells (V79) were irradiated with Carbon K ultrasoft X-rays in the presence and absence of 1M DMSO and frequencies of chromosome aberrations in the first division cells were determined. DMSO reduced the frequencies of exchange types of aberrations (dicentrics and centric rings) by a factor of 2.1–3.5. The results indicate that free radicals induced by ultrasoft X-rays contribute to a great extent to the induction of chromosome aberrations. The possible implications of these results in interpreting the mechanisms involved in the high efficiency of ultrasoft X-rays in the induction of chromosome aberrations are discussed. [Copyright &y& Elsevier]

Dimethylated sulfur compounds play an important role in global sulfur cycle. We investigated the seasonality of dimethylsulfoniopropionate (DMSP), dimethylsulfoxide (DMSO), dimethylsulfide (DMS) and associated processes in two north China marginal seas during 2014 and 2016. High concentrations of DMS, DMSP and DMSO occurred in summer/spring, while the lowest were observed in winter. This clear seasonality was primarily driven by biomass abundance and phytoplankton communities, reflected in chlorophyll a concentrations and the composition/ratios of diatoms and dinoflagellates. The spring maximum was attributed to the annual occurrence of algal bloom. The sea-to-air fluxes of DMS also varied largely between seasons, with an average of 8.84, 11.87, 10.50 and 2.14 μmol m−2 day−1 in spring, summer, autumn and winter, respectively. Given the seasonal uncertainty of sea-to-air flux, the seasonality or situations where specific blooms occur regularly should be considered for accurate estimation of annual global DMS emission. • Strong seasonality driven by biomass abundance and phytoplankton communities were observed for dimethylated sulfur compounds. • Annual occurrence of algal bloom lead to the spring maximum of sulfur compounds in north China marginal seas. • Sea-to-air flux of DMS varied largely between seasons and influences the estimation of annual emissions. [ABSTRACT FROM AUTHOR]

In the coastal areas of southeastern China, high temperatures and humidity in the summer and microfreezing in the winter, as well as a high concentration of salt spray in the environment, seriously deteriorate the durability of asphalt mixtures. Therefore, the microcharacteristics of asphalt mastics (asphalt mixed with mineral filler) under the effect of chlorine salt and "dry–wet and freeze–thaw" (DW-FT) cycles were investigated by Fourier-transform infrared (FTIR) spectroscopy, gel permeation chromatography (GPC), and atomic force microscopy (AFM) techniques. Two factors, including asphalt mastic types (base and styrene-butadiene-styrene (SBS)-modified mastics) and numbers of DW-FT cycles, were considered based on the natural environment. Regression functions were established to explore the relationship between the FTIR, GPC, and AFM indexes. The results indicate that there were no chemical reactions between the asphalt and filler because the infrared spectrum of the base and SBS-modified mastics were similar. With the increase of the salt "DW-FT" cycle numbers, the sulfoxide index and large molecular size ratios ( L M S % ) increased, and the surface roughness ( R q and R a ) of the morphology decreased, as illustrated by a flatting mastics surface phenomenon in the AFM test. Regression analysis confirmed that there was a high correlation between the FTIR, GPC, and AFM indexes, and formation of the bee structures was closely related to the long chain index. The SBS-modified mastics had a better antiaging performance with a lower increase in the sulfoxide index after the salt "DW-FT" cycles in the coastal environment. [ABSTRACT FROM AUTHOR]