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Chuju, a cultivar of Chrysanthemum morifolium, has been traditionally cultivated for over 2000 years in China for both ornamental and medicinal purposes. To date, investigations into the chemical composition of this plant have indicated that it contains compounds with extensive biological activities, although detailed information on the chemical composition of Chuju remains scarce. In the present study, the chemical compositions of Chuju flowers were investigated across five sites in the core Chuju planting area in Anhui province, China. Analytical pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) was used to explore variations in flower chemical fingerprints from different Chuju planting sites. The study identified approximately 200 components in Chuju flowers and stems, including high levels of fatty acids, lipids, polysaccharides and terpenoids. Multivariate statistical analysis indicated that 16 chemical compounds were influential determinants of the chemical fingerprint and could be used to distinguish two clusters in the five core planting areas. The established Py-GC/MS analytical workflow could provide a basis for determining the chemical fingerprints of Chuju and help elucidate that products contain a reproducible content of bioactive compounds and overall quality for potential development of health and medicinal purposes., (© 2024 Wiley-VHCA AG, Zurich, Switzerland.)

Chrysanthemum Flos is the prestigious traditional Chinese medicinal material and the popular health drink. This article comprehensively evaluated the chemical constituents, antioxidant activity, and hepatoprotective effects of 25 common chrysanthemum varieties in China. Firstly, we analyzed the chemical compositions of water extracts of chrysanthemum using UPLC/Q-TOF-MS, and identified 29 chemical components. The results displayed that chrysanthemum was rich in chemical constituents, but there were significant differences in the contents of four phenolic acids and five flavonoids among different varieties, and the coefficient of variation (CVs) ranged from 35.96 % to 114.62 %. Then, the antioxidant activities of different chrysanthemums were investigated, respectively via 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and Ferric Reducing Antioxidant Power (FRAP) assays. The spectrum-effect relationships between nine main components and antioxidant activities were investigated to identify the antioxidant constitutes in chrysanthemums. Meanwhile, H 2 O 2 -induced hepatocyte injury testing showed wide variation in cultivar antioxidant capacity, with Tongchengju (TCJ) producing the best effect (90.32 %), followed by Chuju (CJ; 85.78 %). In addition, the hepatoprotective effects of 8 mainstream varieties were determined by the model of acute alcoholic liver injury. They protected liver from injury by affecting relevant liver function and antioxidant indexes. Huangshangongju (HSG) could decrease aspartate aminotransferase (AST) activity by 39.27 % in liver tissue; Hangju-Fubaiju (HJ-FBJ), Jinsihuangju (JSH), and Chuju (CJ) significantly decreased the malondialdehyde (MDA) content of liver tissue, which reduced by more than 40 %; Jinsihuangju (JSH) of used for tea could double the content of glutathione (GSH) and had the similar effect on superoxide dismutase (SOD) as the positive group, showing significant antioxidant capacity. Therefore, this study confirmed that chrysanthemums are potential resources as antioxidants, functional foods, and medicinal materials. Importantly, it may provide a scientific support for further development and utilization of chrysanthemum, and screen excellent varieties for different demands., (© 2021 Wiley-VHCA AG, Zurich, Switzerland.)

Chrysanthemum morifolium . cv "Hangju" is an important medicinal material with many functions in China. Flavonoids as the main secondary metabolites are a major class of medicinal components in "Hangju" and its composition and content can change significantly after flooding. This study mimicked the flooding stress of "Hangju" during flower bud differentiation and detected its metabolites in different growth stages. From widely targeted metabolomics data, 661 metabolites were detected, of which 46 differential metabolites exist simultaneously in the different growth stages of "Hangju". The top three types of the 46 differential metabolites were flavone C-glycosides, flavonol and flavone. Our results demonstrated that the accumulation of flavonoids in different growth stages of "Hangju" was different; however, quercetin, eriodictyol and most of the flavone C-glycosides were significantly enhanced in the two stages after flooding stress. The expression of key enzyme genes in the flavonoid synthesis pathway were determined using RT-qPCR, which verified the consistency of the expression levels of CHI , F3H , DFR and ANS with the content of the corresponding flavonoids. A regulatory network of flavonoid biosynthesis was established to illustrate that flooding stress can change the accumulation of flavonoids by affecting the expression of the corresponding key enzymes in the flavonoid synthesis pathway.

Background: Chrysanthemi Flos (CF), as a popular traditional Chinese medicine (TCM), has five main cultivars in China, namely "Chuju", "Boju", "Gongju", "Huaiju" and "Hangju". Due to their habitats and processing methods, great quality variations occur yet no systematical study has ever been carried out to evaluate such variations., Purpose: In this study, we aim to establish a new approach that can serve both as a quality control method and as an identification method for cultivars of CF., Method: The components in CF samples were identified by a combination of UPLC-ESI-Q-TOF/MS and GC/MS. Furthermore, a multimodal quantitative method was established by UPLC-UV coupled with principal component analysis (PCA) and the similarity evaluation system (SES), which was used to control and identify four cultivars of CF., Results: 18 compounds of flavonoids and caffeoylquinic acids were identified and ten of them were quantified using UPLC-ESI-Q-TOF/MS. Different cultivars of CF could be clearly distinguished with the fingerprints evaluation and principal component analysis (PCA). A total of 74 volatile compounds were detected by GC/MS. The distinctness of volatile components was observed. By the combination of UPLC-ESI-Q-TOF/MS and GC/MS, an identification and quality control method for CF was successfully established., Conclusion: The combination of UPLC-ESI-Q-TOF/MS and GC/MS could act as a comprehensive multimodal method for both identification and quality control of herbal medicines. This study provided new insights into the overall evaluation method for herbal medicines possessing different cultivars., (Copyright © 2018. Published by Elsevier GmbH.)

Dendranthema morifolium cv. 'jinsidaju', cultivated only in Kaifeng, has been eaten for more than 1000 years. During the antioxidant-activity-guided studies on its chemistry and health care function, two new bisabolane-type sesquiterpenes, (6R,7R)-7-hydroxybisabol-2,9E,11-triene-4-one (jinsidajuol A, 1) and (6R,7R)-7-hydroxy-11-methoxybisabol-2,9E-diene-4-one (jinsidajuol B, 2), and thirteen known compounds (3-15) were isolated from the flowers. Their structures were elucidated by 1D and 2D NMR spectroscopy and HRMS. 1 and 2 are the first example of bisabolane-type sesquiterpenes isolated from the genus Dendranthema. Compounds 6-8, 12 and 13 exhibited strong scavenging activities on the ABTS radical cation with IC 50 3.33, 5.67, 2.00, 2.50, 5.33 μg/mL, respectively. The IC 50 values of all compounds on HepG2 human hepatoma tumor cell line were higher than 50 μM., (Copyright © 2019 Elsevier B.V. All rights reserved.)

A fast, sensitive and reliable method for the determination of fipronil and its metabolites in tea and chrysanthemum was developed using a modified QuEChERS technique and an ultra performance liquid chromatography Q-Exactive Orbitrap mass spectrometry. The mixture of adsorbents containing primary secondary amine (PSA), octadecylsilane (C 18 ) and carbon nanotubes (CNTs), was used as QuEChERS adsorbents. The use of mass resolution at 70000 full width at half maximum (FWHM) and narrow mass windows at 5 ppm achieved high selectivity and repeatability. Satisfactory linearity with correlative coefficient (R 2 ) higher than 0.996 was achieved for all compounds. Recoveries at three levels (2, 10 and 50 μg kg -1 ) ranged from 86% to 112%, while the intra- and inter-day accuracies were less than 15%. Limits of quantification for fipronil and its metabolites were 2 μg kg -1 , which fulfils the requirement of maximum residue limits formulated by European Union and Japan., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

The ingredients of five kinds of Zhejiang's yellow Chrysanthemum morifolium with different flower blossoming stages were comparatively analyzed. Polysaccharides, total flavonoids, volatile oil, alcohol extract, water extract, chlorogenic acid, luteolin, 3,5-O-dicaffeoyl quinic acid and fingerprint of the ingredient were determined as indicators. During flower blossoming stages, the ingredients of Ch. morifolium showed a big difference with a certain variation. At the early opening stage, the contents of flavonoids and volatile oil were higher, the content of chlorogenic acid, luteolin, 3,5-O-dicaffeoyl quinic acid were higher in the middle of the flowers 50% -80% fowers blossoming degree is the optimal time for harvest.

Leaves of Chrysanthemum morifolium were potential medicinal resource. The present study aims to estimate the main bioactive components: total flavonoids (TF), galuteolin (GA), quercitrin (QU), chlorogenic acid (CA) and 3 ,5-O-caffeoylquinic acid ( CQ), which were considered to be the main effective components, in leaves of C. morfolium cultivars in China. The TF content was estimated hy UV-VIS spectrophotometry, while GA, QU, CA, and CQ were quantitatively determined by HPLC. The highest TF content (7. 13% w/w) was found in cultivar Wan Cong (Shexian county). Cultivar Da Bo ( Bozhou county) had the highest GA content (33. 45 mg - g-1); Cultivar Hong Xin (Sheyang county) contained the highest QU content (29.25 mg · g(-1)); Cultivar Chang Ban (Sheyang county) had the highest CA content (13.14 mg ·(-1)). The maximum CQ content (7.35 mg · g(-1)) was observed in culti- r Da Yang ( Tongxiang county). Different cultivars of C. morfolium had significant difference in components, but the leaf and capitulum of C. morifolium. were found to possess similar chemical compositions. The high content of bioactive components in several cultivars suggested the potential utilization of C. morifolium leaves.

Unlabelled: To observe and analyze the character change of Chinese Chrysanthemi Flos from different producing areas in the same storage conditions, and provide certain reference to the study of storage method and storage time of Chrysanthemi Flos., Method: Different species of Chrysanthemi Flos were collected from the whole country at the same period, stored in the same nature conditions for three years. The change on worm situation, mildew degree, flower shape, color and appearance character were ovserved., Result: The degree of worm and mildew situation of different regions of Chrysanthemi Flos was: Qiju < Hangju < Gongju < Chuju < Jiju = Huaiju = Damaya chrysanthemum. Among three cultivar species of Hangju, 'Huju' was the best preserved, following by 'Xiaobaiju', the worst was 'Dabaiju'. After the storage, the degree of worm situation of Gongju was different. The degree of worm situation of Chuju and Qiju were related to the process methods., Conclusion: The storage changes of Chrysanthemi Flos were related to the regions, specise, process methods, geography location and harvest time, etc. Different specises of Chrysanthemi Flos had different storage time.

A multicomponent quantification fingerprint based on HPLC coupled with diode array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI/MS) technique has been established for the analysis of phenolic compounds in 12 samples originated from 5 different cultivars of Chrysanthemum morifolium flowers in China. Four caffeoylquinic acids and 15 flavonoids in the capitulum were identified by comparing the retention times and ultraviolet spectra as well as the mass spectrum and/or matching the empirical molecular formula with that of reference compounds, and the contents of these compounds have been determined simultaneously. The samples from three medicinal cultivars significantly differed in the quality and quantity of flavonoid aglycones and glycosides compared with those from two edible cultivars, which allows the possibility of showing the chemical distinctness of these cultivars and may be useful in their standardization. Moreover, the antiallergic effects of these cultivars were comparatively assayed for the first time. A representative medicinal cultivar, 'huaiju', showed potential activity on the inhibition of antigen-induced degranulation from RBL-2H3 cells and compound 48/80-induced scratching in mice, whereas the in vitro and in vivo antiallergic activities of two edible cultivars were weak. The results suggested that the quality and quantity of some active flavonoid aglycones should be responsible for the pharmacological profiles of these cultivars.

Objective: To compare botanical characteristics of cultivated Chrysanthemum morifolium cv. 'Hangju' of different origins in order to provide the basis for introduction and cultivation of Ch. morifolium cv. 'Hangju'., Method: The characteristics of plants, leaves and capitulum of Ch. morifolium cv. 'Hangju' were measured, and the obtained data were analyzed and compared., Result: The range of plant height was 60.87-99.47 cm, number of branches 2.76-5.20, leave length 4.90-8.40 cm, leave width 3.25-5.38 cm, aspect ratio of leave 1.35-1.83, number of leave split 1.92-3.08. Numbers of capitulum were 21.92-53.12, diameter of capitulum 3.41-5.48 cm, lays of ray florets 3.28-7.16, number of ray florets 55.32-114.60, ray florets length 1.58-2.37 cm, ray florets width 0.50-0.69 cm, aspect ratio of ray florets 2.90-3.99, diameter of tubular flower 1.10-1.58 cm., Conclusion: The botanical characteristics of cultivated Ch. morifolium cv. 'Hangju' were distinguished from different origins. With the cultivation environment change, the botanical characteristics of the cultivars are changed.

This study aimed at identifying the levels of PCBs generated from e-waste recycling, and their potential impacts on the soils and vegetations as well. The ΣPCBs concentrations in soil and plant samples ranged from 7.4 to 4000 ng g(-1) and from 6.7 to 1500 ng g(-1), respectively. For the plant samples, Chrysanthemum coronarium L. from vegetable field and the wild plant Bidens pilosa L. from the burning site showed relatively higher PCB concentrations than other species. For the soil samples, the e-waste burning site had relatively higher PCB concentrations than the adjacent areas, and vegetable soils had higher PCB concentrations than paddy soils. The PCB concentrations showed a clear decreasing trend with the increasing distance from the e-waste recycling site. PCB 28, 99, 101, 138, 153, and 180 were the predominant congeners. Principal component analysis results showed a potential fractionation of PCB compositions from the burning site to the surroundings. The PCB congener pattern at the burning site was similar to Arochlor 1260, pointing to an input of non-domestic e-waste. Similar PCB congeners were found in soils and related vegetables, indicating they derived from the same source. The consumption of vegetables grown in soils near e-waste recycling sites should be strictly avoided due to the high PCBs in the plant tissues., (Copyright © 2011 Elsevier Ltd. All rights reserved.)

Objective: Botanical characters of germplasm resources of Chrysanthemum morifolium for medicine were observed and compared, which could offer reference for its genetic improvement and germplasm resources protection., Method: Based on the random blocks field experiments design, twenty-six morphological traits were observed. The morphological differences among germplasm resources were compared by principal component analysis and cluster analysis., Result: The coefficient of variation values for 17 of 26 traits indicated a high level of variation (above 20%). Six principal components which accounted for 77.14% of total variance were extracted from the principal component analysis. The 29 germplasm resources could be divided into two clusters., Conclusion: There were large morphological variation among germplasm resources on Ch. morifolium for medicine.

Objective: To explore the character of inorganic elements in Flos Chrysanthemi indici and look for relationship between the element concentration and regions., Method: The contents of elements, including borum (B), sodium (Na), magnesium (Mg), phosphorus (P), potassium (K), calcium(Ca), manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), strontium (Sr), cesium (Se), barium(Ba) and lead(Pb) in Chinese traditional herb Flos Chrysanthemi indici from different regions were determined by ICP-AES. The element distrubution diagram were plotted. The principal component analysis and correlation analysis of SPSS were applied for the study of characteristic elements., Result: Similar curves of element concentration have been acquired. It is observed that the content of elements in the samples shows regional diversity. There are 15 correlative element pairs in correlation analysis. Four principal components which accounted for over 84.437% of the total variance were extracted from the original data. The first and second factors accounted for 60.090% of the total variance, which means that P, K, Ca, Mn, Fe, Cu, Sr, B, Na and Se may be the characteristic elements., Conclusion: The showed that element content in Flos Chrysanthemi Indici display special distributing diagram. Remarkable correlation is presented in some element pairs. The elements contents of Flos Chrysanthemi indici gained from Yunan, Hunan are higher than those from other regions.

Objective: To evaluate the quality of Flos Chrysanthemi Indici which produced in twenty-two different producing places., Method: Chlorogenic acid and caffeic acid were analyzed on a Shim-pack C8 colunm (4.6 mm x 250 mm, 5 microm) eluted with the mobile phase consisted of acetonitrile-0.5% phosphoric acid( 19:81). The detection wavelength was set at 326 nm. Linarin were eluted with the mobile phase consisted of methanol-water-acetic acid(26: 23: 1). The detection wavelength was set at 334 nm. The column temperature was 25 degrees C. The flow rate was 1.0 mL x min ., Result: The linear response ranged within 2.5-50 microg for chlorogenic acid (r = 0.998), 2.5-25 microg for caffeic acid (r = 0.998) and 4.97-41.47 microg for linarin (r = 0.999), respectively. Recoveries were 100.8% with RSD 2.1% for chlorogenic acid, 96.2% with RSD 2.3% for caffeic acid and 103.7% with RSD 1.8% for linarin., Conclusion: There was a significant difference in the content of chlorogenic acid, caffeic acid, linarin among the samples. The content of chlorogenic in the sample from Fengdou Chongqing city was the highest in those from other places. The content of caffeic acid in the all samples is very low. The content of linarin in the samples from Jiangsu province and Anhui province almost reached the national standard in pharmacopoeia.

Objective: To analyze the chemical components of the essential oil extracted from six cultivars' Juhua (Zaogongju, Wangongju, Huangyaoju, Chuju, Xiaoboju and Daboju) produced in Anhui province of China, which were breed from Chrysanthemum morifolium, and to provide scientific basis for quality control., Method: The essential oils of the six cultivars'Juhua were extracted by water-steam distillation and separated by GC capillary column chromatography. The components were quantitatively determined with normalization method, and identified by GC-MS., Result: From Zaogongju, Wangongju, Huangyaoju, Chuju, Xiaoboju and Daboju, 247, 226, 246, 182, 216 and 122 chromatographic peaks were detected, among them 75, 54, 78, 50, 53 and 43 components were identified,which were composed of 85.67%, 82.80%, 81.38%, 73.22%, 71.51% and 72.87% of the total essential oil, respectively., Conclusion: Monoterpenoid compounds were higher more than sesquiterpenoid compounds in the five juhua except for Chuju. There was no difference in the constituents of essential oil of Zaogongju and Wangongju. And verbenyl acetate was the main chemical constituent and composed of 32.10% and 37.85% of the total essential oil, respectively. (1R)-Camphor and bisabolol oxide A were the main chemical constituents in Huangyaoju, which were composed of 28.70% and 12.58% of the total essential oil, respectively. beta-Selinene and borneol were the main chemical constituents in Chuju, which were composed of 17.85% and 12.84% of the total essential oil, respectively. Eucalyptol was the main chemical constituent in Xiaoboju, which were composed of 21.33%. Verbene oxides and chrysanthenone were composed of 25.32% and 8.26% of the total essential oil, respectively, in the Daboju. The common chemical constituents were camphene, borneol, bornyl aceate, (1R)-camphor, (-)-4-terpineol, alpha-terpineol, eucalyptol, cis-caryophyllene, caryophyllene oxide, juniper camphor, beta-sesquiphellandrene, alpha-curcumene and beta-farnesene in the six cultivars' Juhua produced in Anhui province of China. The resultant data provided the further evidences to distinguish Juhua' cultivars and scientific basis for quality control of Juhua.

Objective: To analyze the chemical components of the essential oil extracted from five cultivars' Hangjuhua (Zaoxiaoyangju, Chixiaoyangju, Dayangju, Yizhongdabaiju and Xiaotangju), which were breed came from the Chrysanthemum morifolium Ramat, and provide scientific basis for quality control., Method: The essential oil of five cultivars' Hangjuhua were extracted by water-steam distillation and separated by GC capillary column chromatography. The components were quantitatively determined with normalization method, and identified by GC-MS., Result: From the Zaoxiaoyangju, Chixiaoyangju, Dayangju, Yizhongdabaiju and Xiaotangju, 179, 167, 209, 211 and 270 chromatographic peaks were detected, among them 54, 56, 60, 55 and 65 components were identified, which were composed of 73.40%, 64.14%, 65.19%, 53.55% and 62.89% of the total essential oil, respectively., Conclusion: The common chemical constituents were juniper camphor, 1, 3, 3-trimethylcyclohex-1-ene-4-carboxaldehyde, borneol, alpha-curcumene, alpha-bisabolol, cis-caryophyllene, benzyl benzoate, methyl beta, beta-dimethylbenzenepropanoic acid ester, 2, 4-decadienal and heneicosane in the essential oil of the five cultivars' Hangjuhua. Among them, juniper camphor is most which were composed of 11.96%, 10.51%, 10.95%, 13.28% and 10.77% of the total essential oil, and methyl beta, beta-dimethylbenzenepropanoic acid ester is second, which were composed of 1.51%, 1.86%, 2.52%, 4.89% and 2.51% of the total essential oil, respectively. There are marked differences in the compounds contained in the five samples. The juniper camphor is a characteristic constituent in the essential oil of Hangjuhua. The resultant data provided the evidences to establish Hangjuhua' cultivars and scientific basis for quality control of Hangjuhua.

Objective: To study the effects of climate factors on the Chrysanthemum morifolium originated from Wenxian county (i. e "huaiju") for assuring the quality of medicinal C. morifolium., Method: The content of water-soluble extracts, protein, sugar, flavonoid and chlorogenic acid of "huaiju" collected from Henanwenxian, Jiangsunanjing and Zhejiangtongxiang were selected as the indicator and determined, climate data of the corresponding places were collected for studying the effects on "huaiju" quality through path analysis and correlation analysis., Result and Conclusion: There existed no obviously differences among the detected indicators except the soluble sugar content. Sunlight, temperature and precipitation showed more effects on quality of "huaiju", the sunlight had the most direct effect on flavonoid, the 10 cm ground temperature had the most direct effect on chlorogenic acid.

Objective: To study the quality and the chemical components of Chrysanthemum morifolium from Tongxiang city., Method: Chemical constituents of nine cultivars were compared in three types of index: chlorogenic acid, flavonoid and volatile oil., Result and Conclusion: The content varied significantly. The content of chlorogenic acid in Jinjuerhao was 6.66%, the highest among the samples. Yizhongdabaiju showed the highest flavonoid and volatile oil with 9.49% and 3.30 mL x kg(-1) respectively.

Objective: To study the quality and genetic diversity of Chrysanthemun morifolium, the chemical constituents of 20 medicinal chrysanthemum cultivars in China were compared., Method: Chemical constituents of the 20 cultivars were compared in three types of index: chlorogenic acid, flavonoid and volatile oil., Result: There are visible differences on the contents of chlorogenic acid, flavonoid and volatile oil between the cultivars., Conclusion: Each cultivar has its own internal quality, and it is affected by many factors. The chemical constituents of the cultivars showed the genetic diversity in medicinal chrysanthemums of China.