Search

To explore the transformational leadership competency of graduates from one medical university in China and its influencing variables. From 2020 to 2021, 851 medical graduates from seven hospitals affiliated with the Capital Medical University participated in this survey. The authors conducted a cross-sectional study to assess transformational leadership competency, particularly from three aspects, including values, Emotional Intelligence (EI) abilities, and behaviors using the socially responsible leadership scale (SRLS), emotionally intelligent leadership, and student leadership practices inventory (EILI and SLPI). The SRLS scores were medium except for 'controversy with civility'. The EILI scores were medium. The SLPI scores were high except for 'enable others to act' and 'encourage the heart'. The influencing variables of SRLS, EILI, and SLPI were serving as student cadres, serving longer than two semesters (p = 0.01, 0.02 in EILI and SLPI), joining student organizations, participating in social practice, voluntary service (p = 0.001 in SLPI), in training classes for student cadres (p = 0.02, 0.01, 0.02 in SRLS, EILI, and SLPI), and attending lectures on leadership (except for indicated, p < 0.001). Regression analysis showed that attending lectures on leadership was associated with high SRLS, EILI, and SLPI scores (p = 0.04, SRLS; p < 0.001, others), and SRLS and EILI scores could affect SLPI score (F = 2674.44, p < 0.001, R2 = 0.86). Medical graduates' transformational leadership competency at the Capital Medical University was medium measured from values, EI abilities, and behaviors. Group analysis indicated that knowledge learning, organizational involvement, and social/community involvement were associated with leadership capacity building, meanwhile, leaders' values and EI abilities would affect their behaviors, suggesting medical graduates should undertake leadership training from both knowledge learning and practicing. [ABSTRACT FROM AUTHOR]

Background: Headache disorders are widely prevalent and pose a considerable economic burden on individuals and society. Globally, misdiagnosis and inadequate treatment of primary headache disorders remain significant challenges, impeding the effective management of such conditions. Despite advancements in headache management over the last decade, a need for comprehensive evaluations of the status of primary headache disorders in China regarding diagnosis and preventative treatments persists. Methods: In the present study, we analyzed the established queries in the Survey of Fibromyalgia Comorbidity with Headache (SEARCH), focusing on previous diagnoses and preventative treatment regimens for primary headache disorders. This cross-sectional study encompassed adults diagnosed with primary headache disorders who sought treatment at 23 hospitals across China between September 2020 to May 2021. Results: The study comprised 2,868 participants who were systematically examined. Migraine and tension-type headaches (TTH) constituted a majority of the primary headache disorders, accounting for 74.1% (2,124/2,868) and 23.3% (668/2,868) of the participants, respectively. Medication overuse headache (MOH) affected 8.1% (231/2,868) of individuals with primary headache disorders. Over half of the individuals with primary headache disorders (56.6%, 1,624/2,868) remained undiagnosed. The previously correct diagnosis rates for migraine, TTH, TACs, and MOH were 27.3% (580/2,124), 8.1% (54/668), 23.2% (13/56), and 3.5% (8/231), respectively. The misdiagnosis of "Nervous headache" was found to be the most prevalent among individuals with migraine (9.9%, 211/2,124), TTH (10.0%, 67/668), trigeminal autonomic cephalalgias (TACs) (17.9%, 10/56), and other primary headache disorders (10.0%, 2/20) respectively. Only a minor proportion of individuals with migraine (16.5%, 77/468) and TTH (4.7%, 2/43) had received preventive medication before participating in the study. Conclusions: While there has been progress made in the rate of correct diagnosis of primary headache disorders in China compared to a decade ago, the prevalence of misdiagnosis and inadequate treatment of primary headaches remains a veritable issue. As such, focused efforts are essential to augment the diagnosis and preventive treatment measures related to primary headache disorders in the future. [ABSTRACT FROM AUTHOR]

Aim: This study aimed to develop a tool to measure paediatric specialist nurses' core competencies and examine the scale's validity and reliability. Design: An exploratory quantitative study. Methods: This study was performed in April 2022 with 302 paediatric specialist nurses in mainland China. Items were created through a literature review, a qualitative interview, and the Delphi method. The data were evaluated using descriptive statistics, independent sample t‐test, explanatory factor analysis, Pearson correlation coefficient, Cronbach's alpha coefficient and split‐half reliability. Results: The final scale consisted of five factors and 32 items. The factors were communication, coordination and judgement abilities; professional technology mastery ability; specialist knowledge mastery ability; medical‐related processes; and evidence‐based nursing competencies. The explained total variance of the five factors was 62.216%. The scale‐level and item‐level CVI of this scale was 1.00, and the mean CVR of the total scale was 0.788. The Pearson correlation coefficients of each dimension and the total dimension of the scale was 0.709–0.892, and within each dimension, it was 0.435–0.651. The Cronbach's α of this scale was 0.944, and its split‐half reliability was 0.883. [ABSTRACT FROM AUTHOR]

The alpine grassland ecosystem accounts for 53 % of the Qinghai–Tibet Plateau (QTP) area and is an important ecological protection barrier, but it is fragile and vulnerable to climate change. Therefore, continuous monitoring of grassland aboveground biomass (AGB) is necessary. Although many studies have mapped the spatial distribution of AGB for the QTP, the results vary widely due to the limited ground samples and mismatches with satellite pixel scales. This paper proposed a new algorithm using unmanned aerial vehicles (UAVs) as a bridge to estimate the grassland AGB on the QTP from 2000 to 2019. The innovations were as follows: (1) in terms of ground data acquisition, spatial-scale matching among the traditional ground samples, UAV photos, and MODIS pixels was considered. A total of 906 pairs between field-harvested AGB and UAV sub-photos and 2602 sets of MODIS pixel-scale UAV data (over 37 000 UAV photos) were collected during 2015–2019. Therefore, the ground validation samples were sufficient and scale-matched. (2) In terms of model construction, the traditional quadrat scale (0.25 m 2) was successfully upscaled to the MODIS pixel scale (62 500 m 2) based on the random forest and stepwise upscaling methods. Compared with previous studies, the scale matching of independent and dependent variables was achieved, effectively reducing the impact of spatial-scale mismatch. The results showed that the correlation between the AGB values estimated by UAV and MODIS vegetation indices was higher than that between field-measured AGB and MODIS vegetation indices at the MODIS pixel scale. The multi-year validation results showed that the constructed MODIS pixel-scale AGB estimation model had good robustness, with an average R2 of 0.83 and RMSE of 34.13 g m -2. Our dataset provides an important input parameter for a comprehensive understanding of the role of the QTP under global climate change. The dataset is available from the National Tibetan Plateau/Third Pole Environment Data Center (10.11888/Terre.tpdc.272587; H. Zhang et al., 2022). [ABSTRACT FROM AUTHOR]

In most of the world's building material industries, the control of flue gas pollutants mainly focuses on a single pollutant. However, given the large capacity and high contribution of China's building materials industry to global air pollution, the need to develop multi-pollutant emission reduction technology is urgent. Recently, China has focused on reducing the emissions of flue gas pollutants in the building materials industry, established many key research and development projects, and gradually implemented more stringent pollutant emission limits. This project focuses on the most recent advances in flue gas emission control technology in China's building materials industry, including denitration, dust removal, desulfurization, synergistic multi-pollutant emission reduction, and the construction of pilot research and demonstration projects for pollutant removal in several building material industries. On this basis, revised pollutant limits in flue gas emitted in China's building material industry are proposed. [Display omitted] [ABSTRACT FROM AUTHOR]

Objective: The aims were to explore the prevalence and clinical features of fibromyalgia in Chinese hospital patients with primary headache. Background: Studies done in non‐Chinese populations suggest that around one‐third of patients with primary headache have fibromyalgia, but data from mainland China are limited. Investigations into the prevalence and clinical features of fibromyalgia in Chinese patients with primary headache would improve our understanding of these two complex disease areas and help guide future clinical practice. Methods: This cross‐sectional study included adults with primary headache treated at 23 Chinese hospitals from September 2020 to May 2021. Fibromyalgia was diagnosed using the modified 2010 American College of Rheumatology criteria. Mood and insomnia were evaluated employing the Hospital Anxiety and Depression Scale and the Insomnia Severity Index. Results: A total of 2782 participants were analyzed. The fibromyalgia prevalence was 6.0% (166/2782; 95% confidence interval: 5.1%, 6.8%). Compared to primary headache patients without combined fibromyalgia, patients with primary headache combined with fibromyalgia were more likely to be older (47.8 vs. 41.7 years), women (83.7% [139/166] vs. 72.8% [1904/2616]), less educated (65.1% [108/166] vs. 45.2% [1183/2616]), and with longer‐duration headache (10.0 vs. 8.0 years). Such patients were more likely to exhibit comorbid depression (34.3% [57/166] vs. 9.9% [260/2616]), anxiety (16.3% [27/166] vs. 2.7% [70/2612]), and insomnia (58.4% [97/166] vs. 17.1% [447/2616]). Fibromyalgia was more prevalent in those with chronic (rather than episodic) migraine (11.1% [46/414] vs. 4.4% [72/1653], p < 0.001) and chronic (rather than episodic) tension‐type headache (11.5% [27/235] vs. 4.6% [19/409], p = 0.001). Most fibromyalgia pain was in the shoulders, neck, and upper back. Conclusions: The prevalence of fibromyalgia in mainland Chinese patients with primary headache was 6.0%. Fibromyalgia was more common in those with chronic rather than episodic headache. The most common sites of fibromyalgia pain were the neck, shoulders, and back. [ABSTRACT FROM AUTHOR]

Numerous large hydropower projects have been built, are being built, or are planned to be developed in southwest China as a result of the increasing demand for clean energy in China's social and economic development. Based on engineering geological data, site surveys, and the temporal and spatial distribution characteristics of microseismic (MS) events, an MS monitoring system was developed in this study to analyze the stability of the surrounding rock of the spillway tunnel of the Jinchuan hydropower station as well as the fracture and damage mechanism in the concentration zone. The results of the study indicated that the distribution of MS events was correlated with the construction process and geological conditions, that the concentration of MS events and their great moment magnitude could be regarded as signs of future damage to the surrounding rock, and that the surrounding rock of the spillway tunnel primarily exhibited non-shear failure, such as tensile failure, with only a small area exhibiting shear failure. The results can be used as a construction reference and as a forewarning of surrounding rock deformation. [ABSTRACT FROM AUTHOR]

Background: Xinjiang is an economically underdeveloped area in China, but the obesity rate of children and adolescents is increasing year by year. Physical fitness and body mass index (BMI) are very important factors for healthy development, whereas few studies focus on the relationship between them in this region. This study aimed to explore the relationship between physical fitness and BMI of children and adolescents aged 7 to 18 in Xinjiang.Method: A total of 17,356 children and adolescents aged 7-18 years were involved. BMI was divided into five levels by percentiles, from very low to very high. Physical fitness was evaluated by five indicators: grip strength, standing long jump, sit-and-reach, 50 m dash, and endurance running. Single-factor analysis of variance was used to compare the Z-scores of the five physical fitness indicators among different BMI levels for the four age groups by gender. A nonlinear quadratic regression model was used to evaluate the relationship between BMI and each indicator in the four age groups.Result: There is a significant correlation between the five health-related indicators (grip strength, standing long jump, sit and reach, 50 m dash, endurance run) at two age groups (13-15 yrs., 16-18 yrs) of children and adolescents in Xinjiang, China. The range of the Pearson coefficient is 0.048 ~ 0.744. For the other two age groups (7-9 yrs., 10-12 yrs.,) significant correlations are found only in some indicators, and the Pearson coefficient ranges from 0.002 to 0.589. The relationship between BMI and physical fitness presents an U-shaped or inverted U-shaped curve in most age groups(R2 ranges from - 0.001 to 0.182. Children and adolescents with normal BMI score higher on physical fitness tests, and boys (R2 ranges from - 0.001 to 0.182) are more pronounced than girls (R2 ranges from 0.001 to 0.031).Conclusion: Children and adolescents with a BMI above or below the normal ranges have lower physical fitness than those with normal BMI. BMI and physical fitness have an U-shaped or inverted U-shaped curve relationship, and the impact is more evident in boys than girls. Targeted actions such as improving the quality of physical education classes, advocating students to keep a balanced diet and physical exercise should be taken designedly. [ABSTRACT FROM AUTHOR]

Background: There have been a few studies regarding the pre-attack symptoms (PAS) and pre-episode symptoms (PES) of cluster headache (CH), but none have been conducted in the Chinese population. The purpose of this study was to identify the prevalence and features of PAS and PES in Chinese patients, as well as to investigate their relationships with pertinent factors. Methods: The study included patients who visited a tertiary headache center and nine other headache clinics between January 2019 and September 2021. A questionnaire was used to collect general data and information about PAS and PES. Results: Among the 327 patients who met the CH criteria (International Classification of Headache Disorders, 3rd edition), 269 (82.3%) patients experienced at least one PAS. The most common PAS were head and facial discomfort (74.4%). Multivariable logistic regression analysis depicted that the number of triggers (OR = 1.798, p = 0.001), and smoking history (OR = 2.067, p = 0.026) were correlated with increased odds of PAS. In total, 68 (20.8%) patients had PES. The most common symptoms were head and facial discomfort (23, 33.8%). Multivariable logistic regression analysis showed that the number of triggers were associated with increased odds of PES (OR = 1.372, p = 0.005). Conclusions: PAS are quite common in CH patients, demonstrating that CH attacks are not comprised of a pain phase alone; investigations of PAS and PES could help researchers better understand the pathophysiology of CH. [ABSTRACT FROM AUTHOR]

Classification of wetland plant species (PlatSpe) and surface objects (SurfObj) in remote sensing images faces significant challenges due to the high diversity of PlatSpe and the fragmented nature of SurfObj. Unmanned aerial vehicle (UAV) images and satellite images are the primary data sources for the classification of wetland PlatSpe and SurfObj. However, there is still insufficient research on the effect of various data sources and spatial resolutions on the classification results. This study essentially focuses on Huixian Wetland in Guilin, Guangxi, China through utilizing UAV images and satellite images with varying spatial resolutions as data sources. To this end, the MRS_DeepLabV3+ model is constructed based on multi-resolution segmentation and DeepLabV3+, and the wetland PlatSpe and SurfObj are appropriately classified based on this model. The obtained results reveal that: (1) MRS_DeepLabV3+ model with optimal scale parameter (SP) is capable of achieving higher classification accuracy compared to DeepLabV3+. The optimal SPs for both UAV images and satellite images gradually lessen with decreasing the spatial resolution, and satellite images require larger SPs compared to UAV images. (2) In both the UAV and satellite image models, both OA and kappa exhibit a decreasing trend with the reduction of the spatial resolution. (3) The overall classification accuracies of the satellite image models are superior to the UAV image models in the spatial resolution intervals of 2 to 16 m. This investigation can be regarded as a valuable reference for selecting data sources and spatial resolutions in the wetland PlatSpe and SurfObj classification. • MRS_DeepLabV3+ was constructed based on multi-resolution segmentation and DeepLabV3 +. • Analyzed the impact of scale parameters on the MRS_DeepLabV3+ model. • Investigated the effect of spatial resolution on the classification accuracy. • Compared the differences of classification between UAV images and satellite images. [ABSTRACT FROM AUTHOR]

This study was carried out to explore the moderating effect of comic education and locus of control (LOC) in innovation and entrepreneurship education in colleges and universities. Firstly, the theoretical knowledge of comic education, innovation and entrepreneurship education, and LOC was briefly introduced, and the significance of comics for innovation and entrepreneurship education was discussed. Secondly, the existing innovation and entrepreneurship education modes in colleges and universities in China were introduced. Thirdly, a simple comparative analysis was conducted on the internal and external characteristics of LOC. Finally, an investigation was performed on the innovation and entrepreneurship ability of college students. The results demonstrate that the average score of students' innovation spirit is 3.302, with a standard deviation of 0.481, suggesting that the current college students' overall innovative spirit is moderate. Besides, students get moderate scores in each dimension, and the difference between different students is slight. Among them, the mean of reflectiveness is the highest (M = 3.446, SD = 0.540), and the mean of criticality is the lowest (M = 3.160, SD = 0.481). The average score of the current students' entrepreneurial ability is 3.112, indicating that the students' entrepreneurial ability is above the average. From the perspective of each dimension, students have the lowest score in opportunity discovery ability, which is at a low level (M = 2.821, SD = 0.873), while the other five dimensions are at a medium level. The highest is strategic decision-making ability (M = 3.264, SD = 0.749). At the same time, factors such as gender, grade, institution, and students' relatives significantly impact students' innovation and entrepreneurship ability. It can be concluded that colleges and universities should focus on controlling the quality of innovation and entrepreneurship teaching and guiding and carrying out innovation and entrepreneurship practice activities. They should also encourage students to participate in courses and activities related to innovation and entrepreneurship to improve college students' innovation and entrepreneurship ability. [ABSTRACT FROM AUTHOR]

Late blight is a potentially devastating disease of potato. Dimethomorph has been widely used for preventing and controlling this disease. However, there is little information on dimethomorph residue in potato and the production soil. To address this issue, the present study developed a method for accurate analysis of dimethomorph using high-performance liquid chromatography. A linearity that had a determination coefficient of 0.9998 was established based on dimethomorph concentrations from 0.05 to 10.0 mg/L. The recovery rates ranged from 76.61% to 109.11%. The limit of detection and the limit of quantification were 0.02 mg/kg and 0.05 mg/kg, respectively. Potato production experiments were then carried out in three provinces of China, and dimethomorph was applied through a foliar spray. The established method was used for analysing the uptake, dissipation, and residues of dimethomorph in potato leaves and tubers and also in soils. Results showed that foliar spray of dimethomorph was translocated to tubers and generally within 2 days. The half-lives of dimethomorph in potato leaves, tubers, and soil ranged from 2.65 to 4.20, 1.79 to 3.71, and 4.62 to 9.0 days, respectively. The risk assessment of dietary exposure showed that all of the risk index values and the hazard quotient were less than 100%, indicating that foliar spray of dimethomorph on potato at 1.5 times the recommended minimum dosages poses little risk for consumption of the tubers in the three provinces of China. [ABSTRACT FROM AUTHOR]

Grassland classification is crucial for grassland management. One commonly used method utilizes remote sensing vegetation indices (VIs) to map grassland classes at various scales. However, most grassland classifications were conducted as case studies in a small area due to lack of field data sources. At a small scale, classification is reliable; however, great uncertainty emerges when extended to other areas. In this study, large amounts of field observations (more than 30,000 aerial photos) were obtained using unmanned aerial vehicle photography in Inner Mongolia, China, during the peak period of grassland growth in 2018 and 2019. Then, four machine learning classification algorithms were constructed based on characteristic indices of MODIS NDVI in the growing season to map grassland classes of Inner Mongolia. Finally, the spatial distribution and temporal variation of temperate grassland classes were analyzed. Results showed that: (1) Among all characteristic indices, the maximum, average, and sum of MODIS NDVI from July to September during 2015 to 2019 greatly affected grassland classification. (2) The random forest method exhibited the best performance with overall accuracy and kappa coefficient being 72.17% and 0.62, respectively. (3) Compared with the grassland class mapped in the 1980s, 30.98% of grassland classes have been transformed. Our study provides a technological basis for effective and accurate classification of the temperate steppe class and a theoretical foundation for sustainable development and restoration of the temperate steppe ecosystem. [ABSTRACT FROM AUTHOR]

The species composition of eight shrub communities were investigated in order to understand the species diversity of plant communities in buffer zone and wetland of Momianhe stream along a long-term mine waste area, Lanping county, Yunnan province, China. Dominant plant species and soil samples were collected to analysis heavy metal (Cu, Zn, Pb and Cd) accumulation characteristics. The results showed that 100% samples for Zn, Pb, Cd, and 87.5% samples for Cu in the investigated area exceeded the Yunnan geochemical background value of the heavy metals in the soil. There were 36 plants species in communities, among which Epilobium pyrricholophum, Elsholtzia argyi, Artemisia vestita, Tripogon chinensis were the dominant species. Plant species, the number of individuals, Ecological Dominance (Do), Shannon–Wiener index (H′), Simpson diversity index (Dsi) and Pielou evenness index (Epi) were affected by Cd and Cu contents of the soil and sediment. Therefore, the results indicate that Cu and Cd contents and ecological risk in the process of long-term vegetation restoration of small catchment in lead–zinc mine waste area should pay more attention. [ABSTRACT FROM AUTHOR]

It is of great significance for the coordinated development of the environment and the economy to study the impact of the human driving factors of land use change (LUC) on ecosystem service value (ESV). In this study, we combined the biomass and remote sensing data of the Lijiang River Basin (LRB), which is a typical karst basin with a fragile ecological environment, to establish an ESV model to calculate the ESV. We also introduced the Lorenz curve and Gini coefficient to further analyze the impacts of the human driving factors of LUC on ESV. The results show that (1) the ESV in the LRB from 1995 to 2015 decreased from 8640.03 million yuan to 8595.38 million yuan, with a total decrease of 44.65 million yuan, indicating that the overall ESV in the region has a decreasing trend; (2) the obvious changes in land use caused a significant loss in ESV and changes in the structure of ecosystem services; and (3) the human driving factors of the total population, GDP, and urbanization rate are inversely related to the ESV in the LRB. [ABSTRACT FROM AUTHOR]

To the Editor, As an international health problem, allergic rhinitis (AR) currently affects 10%-40% of the global population.1 Multicenter studies conducted in Europe and America have revealed the clinical features of pediatric, adolescent, and adult patients with AR, including the subtype percentages, common sensitizing allergens, frequent comorbidities, and methods of treatment2,3; however, few studies have reported such information for children or the general population in the cities or provinces of China.4,5 Therefore, there remains an unmet need to acquire more reliable data concerning the manifestations of AR in China and the characteristics of Chinese AR patients. Consequently, AR patients with a lower education level were more likely to have ever or be currently receiving allergen immunotherapy than were patients with a higher educational degree ( I X i SP 2 sp = 14.30, I P i < .01, Figure S5). A multinomial logistic regression analysis (Table 1) showed that PM, IMS, and PMS AR patients were less likely to suffer from coronary heart disease than were IM patients. Finally, when compared with IM patients, the IMS patients more often had a family history of allergy (OR: 1.35, 95% CI: 1.06-1.71), and PMS patients were less likely to be nonsmokers and more likely to have a drug allergy (OR: 1.19, 95% CI: 1.03-1.38; OR: 1.39, 95% CI: 1.03-1.88, respectively). [Extracted from the article]

Introduction: Patients who come to the emergency department because of acute psychiatric symptoms are often not admitted to the correct department timely. The purpose of this study is to identify the clinical characteristics of patients with acute psychiatric symptoms in order to achieve early and correct triage in the emergency room. Methodology: We conducted a cross-analysis of inpatients who first came to the emergency department with acute psychiatric symptoms and then admitted to the department of neurology or psychiatry between years 2012 and 2018. Among them, 70 patients were rediagnosed and retransferred, with 38 patients to the department of neurology and 32 patients to the department of psychiatry. The clinical characteristics, laboratory examination, and Neuropsychiatric Inventory (NPI) were analyzed. Results: Patients who were rediagnosed with neurological diseases were more prone to have somatic symptoms (headache, dizziness) (P < 0.05). Because of the atypical early clinical manifestations in the emergency room, fever and positive neurological signs do not necessarily represent the diagnosis of neurological diseases. On the other hand, the absence of these manifestations does not guarantee the diagnosis of psychiatric illness. According to NPI, abnormal behaviors, changes in appetite, and sleep disturbances were more obvious in patients with neurological diseases (P < 0.05), whereas patients with psychiatric disorders often showed prominent irritability (P < 0.05). Conclusions: Acute psychiatric symptoms are usually complex and diverse. The triage and diagnosisshould be based on multiple factors. After triage, clinical symptoms should be dynamically observed. [ABSTRACT FROM AUTHOR]

High workloads and heavy academic pressure can have significant implications for the risk for depression and poor quality of life (QoL). This study aimed to investigate QoL and depressive symptoms in medical students undergoing postgraduate neurology specialty training in China. The survey covered demographic characteristics, the 8-itemMedical Outcomes Study Short-Formquestionnaire (SF-8), and the 2-itemPrimary Care Evaluation of Mental Disorders depression screening tool. Logistic regression analysis was used to investigate the determinants of QoL and depressive symptoms. Participants were 1,814 postgraduates from 249 hospitals in 27 Chinese provinces. The mean SF-8 physical and mental component summary scores were 78.17 (standard deviation [SD] 15.20) and 68.33 (SD 17.15), respectively. One-third of respondents had depressive symptoms, and those without depressive symptoms had significantly higher QoL scores. The multivariate regression analysis showed that factors independently associated with depressive symptoms were being in the second year of study, a lower household income, and less sleep time. Although QoL among our sample of Chinese medical students undergoing postgraduate neurology specialty training was favorable relative to other comparable populations, one-third of respondents had depressive symptoms. Accurate measures should be taken to change this situation. [ABSTRACT FROM AUTHOR]

Non‐syndromic oculocutaneous albinism (nsOCA) is a group of genetically heterogeneous autosomal recessive disorders with complete lack or decrease pigmentation in skin, hair, and eyes. TYR, OCA2, TYRP1, SLC45A2, SLC24A5, and LRMDA were reported to cause OCA1‐4 and OCA6‐7, respectively. By sequencing all the known nsOCA genes in 114 unrelated Chinese nsOCA patients combined with In silico analyses, splicing assay, and classification of variants according to the standards and guidelines of American College of Medical Genetics and Genomics, we detected seventy‐one different OCA‐causing variants separately in TYR, OCA2, SLC45A2, and SLC24A5, including thirty‐one novel variants (13 in TYR, 11 in OCA2, and 7 in SLC45A2). This study shows that OCA1 is the most common (75/114) and OCA2 ranks the second most common (16/114) in Chinese. 99 patients of our cohort were caused by variants of all the known nsOCA genes. Cutaneous phenotypes of OCA1, OCA2, and OCA4 patients were shown in this study. The second OCA6 case in China was identified here. These data expand the spectrum of OCA variants as well phenotype and facilitate clinical implement of Chinese OCA patients. [ABSTRACT FROM AUTHOR]

The purpose of this study was to investigate the effect of a self-monitoring intervention on the independent completion of dishwashing for three boys with autism (ages 6, 7, and 8) in China. The self-monitoring intervention included visual task analysis, in vivo modeling, self-recording, video self-feedback, and reinforcement. A multiple probe across subjects design was used. Prior to the study, the children had limited or no dishwashing skills, nor did they receive any training on self-monitoring. All three children acquired dishwashing and performed the task independently without supervision one week after the intervention. Their parents were very satisfied with this intervention. [ABSTRACT FROM AUTHOR]

In view of the seriously-polluted water and broken eco-waterscapes in the Qiusuo Brook on campus of China Three Gorges University, this paper proposed the ecological restoration concept and mode of campus river according to field investigation and monitoring results, i.e. "reduce exogenous pollution--restore sediment microorganism--build intensified plant filter strip--build river water ecosystem and optimize landscapes--maintain ecological river course". In addition to introducing the design concept and principle of this mode, this paper specified the concrete projects such as sewage interception and water supplement, restoration of sediment microoiganism, restoration of water plants, ecological restoration of embankment etc., and also gave planar layouts and sketches of some projects, in order to provide references for the researches and practices of campus river ecological restoration in China Three Goiges University and other similar projects. [ABSTRACT FROM AUTHOR]

Pikas ( Ochotona curzoniae ) are common on the Qinghai-Tibetan Plateau (QTP). However, little is known about the effect of their burrowing activity on carbon emissions from alpine grassland ecosystems. In this study, we investigated soil organic carbon (SOC), total nitrogen (TN) density and ecosystem CO 2 flux from the original vegetation and pika piles of three typical alpine grasslands in two climate regions. SOC loss induced by pika activity varied among grassland types. Alpine meadow with high SOC was susceptible to pika activity. Ecosystem CO 2 flux from pika piles with open holes was significantly higher than that from the original vegetation ( P < 0.05, n = 4). When holes near pika piles were blocked, ecosystem CO 2 flux from pika piles was not significantly different from that of the original vegetation ( P > 0.05, n = 4). Our results suggested that CO 2 dissipates into pika tunnels and is easily emitted into the atmosphere through pika holes. Due to the large amount of pika holes in alpine grasslands, their significant effect on ecosystem CO 2 flux should not be ignored in future studies. [ABSTRACT FROM AUTHOR]

The article presents a study which describes desorption behavior and anisotropy of coalbed gas in high rank coal reservoir in the Zhengzhuang Block of the Southern Qinshui Basin, China. Primary measurements and methods used to investigate the 93 core from the 13 wells were offered. Results indicate the factors influencing the adsorption time including gas content, critical desorption pressure, and total densities and determine a strong anisotropy within coal reservoir.

Different geographical plant populations within a single species can exhibit variation, in the production of secondary metabolites. Genetic and environmental variations both contribute to differences between populations; however, the relative importance of these factors is unclear. Here, the extent of variation in the production of four flavonoid glycosides (epimedin A, B, C and icariin) were investigated in eleven wild populations of Epimedium sagittatum used in traditional Chinese medicine. Secondary metabolite profiles were classified into five chemotypes. A common garden experiment indicated this chemotype variation has a significant genetic basis. Extensive genetic variation among intraspecific populations was shown using a retrotransposon-based molecular marker system. These results will assist in development of strategies for conservation, utilization and domestication of E. sagittatum . [ABSTRACT FROM AUTHOR]

Background: Several epidemiological surveys of allergic rhinitis (AR) have been conducted in China. However, the clinical features of AR are still not clear enough. The aim of the current study was to perform a multicenter investigation to evaluate the clinical features of AR in China. Methods: A multicenter investigation was performed in 13 allergy centers in central China. A disease-related questionnaire was completed by each patient themselves or with guardian assistance after the diagnosis of AR. The clinical features of AR and allergen profile were analyzed. Results: Eleven thousand four patients who were diagnosed with AR were recruited in this study. The percentages of classification of AR according to the Allergic Rhinitis and Its Impact on Asthma guidelines were 9.7% intermittent mild (IM), 3.1% persistent mild (PM), 33.9% intermittent moderate-severe (IMS), and 53.3% persistent moderate-severe (PMS). There were 61.6 and 42.2% AR patients who had concomitant ocular or lower respiratory symptoms in clinic. The occurrence of ocular and lower respiratory symptoms was found to be gradually increased from IM, PM, and IMS to PMS. Cold air and temperature change were the two most common factors triggering the nasal symptoms. Dermatophagoides pteronyssinus and Dermatophagoides farinae were the most important allergens of central China. Conclusions: This study has contributed to a better understanding of clinical features of AR in China. [ABSTRACT FROM AUTHOR]

Three strains of H6N6 subtype avian influenza virus (AIV) were isolated from live-poultry markets of central China during 2009-2010. A phylogenetic analysis showed that these isolates originated from gene reassortment among different virus lineages of the H6 subtype. In an experimental infection of animals, the selected isolate was non-pathogenic for chickens and low-pathogenic for mice. The wild-type isolate was capable of replication in mouse lung without prior adaptation, and the virulence to mice increased rapidly during adaption in mouse lung. The genomes of viruses of passage 0 (P0), P4, and P8 were sequenced and compared, and virulence-related amino acid substitutions were found in multiple sites during mouse lung passage. [ABSTRACT FROM AUTHOR]

As a newly emerged development zone model featuring cross-region cooperation and collaborative construction, Joint Development Zone (hereafter referred to as JDZ) is an important spatial platform for promoting industrial transfer, regional cooperation, and regional economic integration. Constructing JDZs through cross-regional cooperation may, to a certain extent, ease the pressures associated with factor-tense and industry transformation in the development of China's developing zones. This would help the regional industry and allow regional cooperation to shift to regional integration. The JDZ in the Yangtze River Delta region has outperformed the other JDZs in China. As of 2011, over 40 JDZs have been built in Jiangsu and Zhejiang province, including China-Singapore Suzhou Industrial Park (SIP), Suzhou-Suqian Industrial Park, etc. Over 20 JDZs have been built in areas adjacent to Shanghai, including the Caohejing Hi-Tech Development Zone Haining Partition. Meanwhile, Anhui has set up the Yangtze Delta JDZ in the Wan River Industrial Transfer Demonstration Zone. JDZ is playing an increasingly important role in industry transfer and regional integration. The home side administration Authority of JDZ coalizes cross-regionally with the gust administrative or market players that are advantage-complementary through joint planning, construction, management and tax distribution in certain region in the local side. The home side shall provide institution innovation revenue to the guest side, management or enterprises, to encourage oriented industry transfer and help agglomeration, thus to enhance the development of both side and win-win result. It has three main effects, demonstration effect of industry transfer and upgrading, revenue returning effect and industry update and platform effect of regional cooperation and coordinated development. The key to success of JDZ are the options and innovations of the management model. The management model can be extracted according to its functional features of planning management and development and forms a three level pyramid. This pyramid is composed of a local administration coordination body for both sides, an administration body for the JDZ itself and the group of investors to the JDZs. As has been shown, the status and function in the specific management model of the three levels determines the difference of the functional direction of the management as a whole and has a strong impact on the goal, location, function distribution and interest distribution of the management model. Accordingly, the management model can be also defined as developing-oriented and opening-oriented, with each having their own advantages and disadvantages. As the management model itself is still in the process of improving and innovating, putting forward a balanced management model emphasizing both developing and third-party participation in opening features is highly anticipated. [ABSTRACT FROM AUTHOR]

Background and Aims Wild soybean (Glycine soja), a native species of East Asia, is the closest wild relative of the cultivated soybean (G. max) and supplies valuable genetic resources for cultivar breeding. Analyses of the genetic variation and population structure of wild soybean are fundamental for effective conservation studies and utilization of this valuable genetic resource. Methods In this study, 40 wild soybean populations from China were genotyped with 20 microsatellites to investigate the natural population structure and genetic diversity. These results were integrated with previous microsatellite analyses for 231 representative individuals from East Asia to investigate the genetic relationships of wild soybeans from China. Key Results Analysis of molecular variance (AMOVA) revealed that 43·92 % of the molecular variance occurred within populations, although relatively low genetic diversity was detected for natural wild soybean populations. Most of the populations exhibited significant effects of a genetic bottleneck. Principal co-ordinate analysis, construction of a Neighbor–Joining tree and Bayesian clustering indicated two main genotypic clusters of wild soybean from China. The wild soybean populations, which are distributed in north-east and south China, separated by the Huang-Huai Valley, displayed similar genotypes, whereas those populations from the Huang-Huai Valley were different. Conclusions The previously unknown population structure of the natural populations of wild soybean distributed throughout China was determined. Two evolutionarily significant units were defined and further analysed by combining genetic diversity and structure analyses from Chinese populations with representative samples from Eastern Asia. The study suggests that during the glacial period there may have been an expansion route between south-east and north-east China, via the temperate forests in the East China Sea Land Bridge, which resulted in similar genotypes of wild soybean populations from these regions. Genetic diversity and bottleneck analysis supports that both extensive collection of germplasm resources and habitat management strategies should be undertaken for effective conservation studies of these important wild soybean resources. [ABSTRACT FROM AUTHOR]

Abstract: H5N1 highly pathogenic avian influenza (HPAI) viruses have seriously affected the Asian poultry industry since their recurrence in 2003. While surveillance in southern China has revealed that H5N1 viruses underwent extensive genetic reassortment to generate many different viral genotype viruses, little is known concerning the genotypes of H5N1 virus that circulated in central China in recent years. In this study, 16 H5N1 influenza viruses were isolated from the poultry market in central China during late 2006 and early 2007, and the genotypes and pathogenicity of the viruses were identified and characterized. All eight segments of each virus were sequenced, and phylogenetic analysis showed that the two surface glycoprotein genes, hemagglutinin (HA) and neuraminidase (NA), of all the viruses were closely related to the H5N1 viruses isolated in poultry in southern China since 2006. Phylogenetic analysis of the internal protein genes indicated that four viral genotypes circulated in poultry markets in central China. The virulence of 7 of the 16 isolates was tested in chickens and mice. The results showed that the 7 isolates were highly pathogenic for SPF chickens, and had a varied virulence in mice. Our results indicate that the H5N1 viruses circulated in central China have diversified characteristics of genotype and virulence. [Copyright &y& Elsevier]

Abstract: In this study, we identified a sex-specific DNA marker using randomly amplified polymorphic DNA (RAPD) fingerprinting in Yellow River carps (Cyprinus carpio from the Yellow River). Two hundred and twenty random primers were used in pooled DNA samples and individual DNA samples from male and female fish for RAPD fingerprinting. When using the primer S2107, a novel sex-specific PCR product was identified in all male individuals. DNA sequencing revealed that this 909 bp long DNA fragment has a low similarity to a repetitive sequence in zebrafish Danio rerio. In order to confirm the amplification results, two primers were designed within the male-specific sequence in order to amplify the sex-specific fragment from genomic DNA of male and female carps for sexing by PCR. The results indicated bands specific for males but not females. Respective results were obtained in dot blot and Southern blot hybridization experiments when using this sex-specific fragment as the probe. The sex-specific pattern was observed in 30 individuals from three separate common carp stocks, suggesting that the sequence is conserved in common carp species. However, no hybridization signals were found in grass carps Ctenopharyngodon idella, which are related to common carps. We submit to use this sex-specific fragment as a marker to rapidly and accurately identify the gender of Yellow River C. carpio. Furthermore, the sex-specific chromosome region may be characterized and used to study mechanisms of chromosome evolution in this fish species. [Copyright &y& Elsevier]

Simple Summary: The fungus gnat (Bradysia odoriphaga Yang and Zhang) is a major pest of chive (Allium tuberosum Rottl. ex Spreng) that can cause more than 50% yield losses during chive production in China. The neonicotinoid, neuroactive insecticide clothianidin has been widely used for chive gnat control; however, following intensive use of this compound, its effects on chive gnat have been markedly reduced, possibly due to the development of insecticide resistance. Hexaflumuron is an insect growth regulator which disrupts chitin synthesis during molting by inhibiting the incorporation of N-acetyl glucosamine monomers into the integument chitin of insects. The present study shows that co-drenching of clothianidin and hexaflumuron enhanced chive absorption of clothianidin, resulting in significant improvement in control of fungus gnat. Additionally, the terminal residues of clothianidin in chive were lower than the maximum residue limit in chive set by the Codex Alimentarius Commission, hence, the chive could be safe for consumption. The fungus gnat is a major pest of chive in China. Its control has been relied heavily on the application of clothianidin. Due to the intensive application, its control efficacy become reduced. The present study was intended to evaluate co-drenching of clothianidin with hexaflumuron on absorption and dissipation of clothianidin in chive plants and soils and determine the effect of such application on control efficacies. Chive production fields in Guangdong and Hubei Provinces were drenched with clothianidin alone and a mixture of clothianidin and hexaflumuron at low application rates. Concentrations of clothianidin in chive plants and soils were analyzed by HPLC. Results showed that co-application had higher control efficacies against the fungus gnat than clothianidin alone. The co-application enhanced clothianidin absorption and dissipation and extended the half-lives of clothianidin in chive. It was likely that hexaflumuron protected chive roots from larva damage, and healthy roots absorbed more clothianidin, resulting in the extension of the half-lives. Additionally, the terminal residues of clothianidin in chive after 14 days of application were lower than the maximum residue limit in chive set by the Codex Alimentarius Commission. This study for the first time documented that co-application of clothianidin and hexaflumuron improved chive plants in absorption and dissipation of clothianidin and enhanced fungus gnat control efficacies. [ABSTRACT FROM AUTHOR]

The source region of the Yellow River (SRYR) is an important water conservation and animal husbandry resource in China. It is of great significance to understand the patch pattern and ecological risk of alpine grassland in the SRYR for ecological environment management. This study first used 12 unmanned aerial vehicle (UAV) aerial images and eight moderate resolution imaging spectroradiometer (MODIS) vegetation index product MOD13Q1 images from July to August in 2019 to extract alpine grassland patch patterns in the SRYR, then constructed an ecological risk model based on the landscape vulnerability index and landscape disturbance index, and finally combined spatial self-reliance correlation and semi-variance analysis methods to explore the spatial distribution of ecological risks. The results showed that the patch fragmentation degree (Pi), area weighted shape index (AWMSI), and separation degree (Si) of the four grassland types in the SRYR are ordered as follows: alpine steppe > degraded meadow > alpine meadow > swamp meadow. Moreover, the greater the fractional vegetation cover (FVC), the greater the landscape dominance index (DOi), and the better the ecosystem stability. The spatial difference of ecological risk in the SRYR shows a situation of low risk in the east (ERImin = 1.5355) and high risk in the west (ERImax = 70.6429). High FVC was found in low and mild low risk areas where the vegetation types are mainly swamp meadow and shrub, while low FVC was found in high and mild high-risk areas where the vegetation types are mainly alpine steppe and degraded meadow. The spatial distribution of ecological risk of the SRYR has obvious positive spatial correlation (Moran's I = 0.863), the spatial aggregation distribution is distinct, and the local space has significant high-high aggregation and low–low aggregation phenomena. The results of this study reveal that patch characteristics have good indicative significance for alpine grassland ecological protection and should be considered in future studies. In addition, the ecological risk in the SRYR is relatively high, especially in the western region, which should be taken seriously in future ecological management and governance. [ABSTRACT FROM AUTHOR]

For the estimation of weak echo with low signal-to-noise ratio (SNR), a multilag estimator is developed, which has better performance than the conventional method. The performance of the multilag estimator is examined by theoretical analysis, simulated radar data and some specific observed data collected by a C-band polarimetric radar in previous research. In this paper, the multilag estimator is implemented and verified for Nanjing University C-band polarimetric Doppler weather radar (NJU-CPOL) during the Observation, Prediction and Analysis of Severe Convection of China (OPACC) field campaign in 2014. The implementation results are also compared with theoretical analysis, including the estimation of signal power, spectrum width, differential reflectivity, and copolar correlation coefficient. The results show that the improvement of the multilag estimator is little for signal power and differential reflectivity, but significant for spectrum width and copolar correlation coefficient when spectrum width is less than 2 ms−1, which implies a large correlation time scale. However, there are obvious biases from the multilag estimator in the regions with large spectrum width. Based on the performance analysis, a hybrid method is thus introduced and examined through NJU-CPOL observations. All lags including lag 0 of autocorrelation function (ACF) are used for moment estimation in this algorithm according to the maximum usable lag number. A case study shows that this hybrid method can improve moment estimation compared to both conventional estimator and multilag estimator, especially for weak weather echoes. The improvement will be significant if SNR decreases or the biases of noise power in the conventional estimator increase. In addition, this hybrid method is easy to implement on both operational and non-operational radars. It is also expected that the proposed hybrid method will have a better performance if applied to S-band polarimetric radars which have twice the maximum useable lags in the same conditions with C-band radars. [ABSTRACT FROM AUTHOR]

Avian orthoavulavirus 13 (AOAV-13), also named avian paramyxovirus 13 (APMV-13), has been found sporadically in wild birds around the world ever since the discovery of AOAV-13 (AOAV-13/wild goose/Shimane/67/2000) in a wild goose from Japan in 2000. However, there are no reports of AOAV-13 in China. In the present study, a novel AOAV-13 virus (AOAV-13/wild goose/China/Hubei/V93-1/2015), isolated from a wild migratory waterfowl in a wetland of Hubei province of China, during active surveillance from 2013 to 2018, was biologically and genetically characterized. Phylogenetic analyses demonstrated a very close genetic relationship among all AOAV-13 strains, as revealed by very few genetic variations. Moreover, pathogenicity tests indicated that the V93-1 strain is a low virulent virus for chickens. However, the genome of the V93-1 virus was found to be 16,158 nucleotides (nt) in length, which is 12 nt or 162 nt longer than the other AOAV-13 strains that have been reported to date. The length difference of 12 nt in strain V93-1 is due to the existence of three repeats of the conserved sequence, "AAAAAT", in the 5′-end trailer of the genome. Moreover, the HN gene of the V93-1 virus is 2070 nt in size, encoding 610 aa, which is the same size as the AOAV-13 strain from Japan, whereas that of two strains from Ukraine and Kazakhstan are 2080 nt in length, encoding 579 aa. We describe a novel AOAV-13 in migratory waterfowl in China, which suggests that diversified trailer region sequences and HN gene lengths exist within serotype AOAV-13, and highlight the need for its constant surveillance in poultry from live animal markets, and especially migratory birds. [ABSTRACT FROM AUTHOR]

Plums (Prunus spp.) are important deciduous fruit crops in the world. China is a major producer of P. salicina Lindl., but the genetic relationship of Chinese plums in key production regions remain unclear. In this study, 14 University of British Columbia (UBC) inter simple sequence repeats (ISSR) primers were used to analyze 33 plum varieties cultivated in Fujian Province to determine their genetic diversity and population structure. A total of 146 bands were generated, of which 130 were polymorphic. Mean percentage of polymorphic bands was 89.04%, Shannon's information index value was 0.38, and the Nei's genetic index value was 0.24. Using unrooted trees (Neighbor-Joining method), 33 varieties were classified into four groups. Split graph separated them into two major groups, each with two subgroups. The two phylogenetic trees indicate that environmental or natural selection pressure is an important factor influencing their genetic relationship. Analysis of population structure revealed that they have frequent genetic exchanges among closed subpopulations; thus, genetic variation mainly occurs within the population. Additionally, based on the phylogenetic analysis and unique morphological characteristics of fruits, we propose that the Chinese landrace Nai could contribute significantly to development of the famous variety Wickson. [ABSTRACT FROM AUTHOR]

RESEARCH HIGHLIGHTSThe Avulavirus within the family Paramyxoviridae includes at least 22 different species, and is known to cause different types of infections and even be fatal in multiple avian species. There is limited knowledge of the genetic and biological information of Avulavirus species 2 to 22 in domestic and wild birds and the disease significance of these viruses in birds is not fully determined, although as many as 10 new distinct species have been identified from wild birds and domestic poultry around the world in the last decade. This study aimed to use PCR, virus isolation, and sequencing to genetically and biologically characterize Avian Orthoavulavirus 16 (AOAV-16) in wild birds and domestic poultry collected from different locations in China between 2014 and 2022. Of five isolated AOAV-16 strains (Y1 to Y5), only the Y4 strain had a haemagglutination (HA)-negative result. All of these isolates were low virulent viruses for chickens, except Y3 which was detected simultaneously with avian influenza virus (AIV) of H9N2 subtype. Furthermore, at least four different types of intergenic sequences (IGS) between the HN and L genes junction, and recombination events, as well as interspecific transmission by wild migratory birds, existed within the species AOAV-16. These findings and results of other reported AOAV-16 strains recommend strict control measures to limit contact between wild migratory birds and domestic poultry and imply potential threats to commercial poultry and even public health challenges worldwide.First confirmation of AOAV-16 in domestic and wild birds in China.AOAV-16 are low virulent viruses for chickens.Co-circulation/co-infection of AOAV-16 and H9N2 subtype AIV enhanced pathogenicity.Different intergenic sequences and recombination events exist within AOAV-16.First confirmation of AOAV-16 in domestic and wild birds in China.AOAV-16 are low virulent viruses for chickens.Co-circulation/co-infection of AOAV-16 and H9N2 subtype AIV enhanced pathogenicity.Different intergenic sequences and recombination events exist within AOAV-16. [ABSTRACT FROM AUTHOR]

Background: The clinical profile of cluster headache may differ among different regions of the world, warranting interest in the data obtained from the initial Chinese Cluster Headache Register Individual Study (CHRIS) for better understanding. Methods: We conducted a multicenter, prospective, longitudinal cohort study on cluster headache across all 31 provinces of China, aiming to gather clinical characteristics, treatment approaches, imaging, electrophysiological and biological samples. Results: In total 816 patients were enrolled with a male-to-female ratio of 4.33:1. The mean age at consultation was 34.98 ± 9.91 years, and 24.89 ± 9.77 years at onset. Only 2.33% were diagnosed with chronic cluster headache, and 6.99% had a family history of the condition. The most common bout was one to two times per year (45.96%), lasting two weeks to one month (44.00%), and occurring frequently in spring (76.23%) and winter (73.04%). Of these, 68.50% experienced one to two attacks per day, with the majority lasting one to two hours (45.59%). The most common time for attacks was between 9 am and 12 pm (75.86%), followed by 1 am and 3 am (43.48%). Lacrimation (78.80%) was the most predominant autonomic symptom reported. Furthermore, 39.22% of patients experienced a delay of 10 years or more in receiving a correct diagnosis. Only 35.67% and 24.26% of patients received common acute and preventive treatments, respectively. Conclusion: Due to differences in ethnicity, genetics and lifestyle conditions, CHRIS has provided valuable baseline data from China. By establishing a dynamic cohort with comprehensive multidimensional data, it aims to advance the management system for cluster headache in China. [ABSTRACT FROM AUTHOR]

Approximately 100 migratory birds, including whooper swans and pochards, were found dead in the Sanmenxia Reservoir Area of China during January 2015. The causative agent behind this outbreak was identified as H5N1 highly pathogenic avian influenza virus (HPAIV). Genetic and phylogenetic analyses revealed that this Sanmenxia H5N1 virus was a novel reassortant, possessing a Clade 2.3.2.1c HA gene and a H9N2-derived PB2 gene. Sanmenxia Clade 2.3.2.1c-like H5N1 viruses possess the closest genetic identity to A/Alberta/01/2014 (H5N1), which recently caused a fatal respiratory infection in Canada with signs of meningoencephalitis, a highly unusual symptom with influenza infections in humans. Furthermore, this virus was shown to be highly pathogenic to both birds and mammals, and demonstrate tropism for the nervous system. Due to the geographical location of Sanmenxia, these novel H5N1 viruses also have the potential to be imported to other regions through the migration of wild birds, similar to the H5N1 outbreak amongst migratory birds in Qinghai Lake during 2005. Therefore, further investigation and monitoring is required to prevent this novel reassortant virus from becoming a new threat to public health. [ABSTRACT FROM AUTHOR]

[Display omitted] Migratory birds are considered natural reservoirs of avian influenza A viruses (AIVs). To further our viral ecology knowledge and understand the subsequent risk posed by wild birds, we conducted a 4-year surveillance study of AIVs in the bird wintering wetlands of the Yangtze River, China. We collected over 8000 samples and isolated 122 AIV strains. Analyses were then carried out with 108 novel sequenced genomes and data were deposited in GISAID and other public databases. The results showed that the Yangtze River wintering wetlands functioned as a mixing ground, where various subtypes of AIVs were detected harboring a high diversity of nucleotide sequences; moreover, a portion of AIV gene segments were persistent inter-seasonally. Phylogenetic incongruence presented complex reassortment events and distinct patterns among various subtypes. In addition, we observed that viral gene segments in wintering wetlands were closely related to known North American isolates, indicating that intercontinental gene flow occurred. Notably, highly pathogenic H5 and low pathogenic H9 viruses, which usually circulate in poultry, were found to have crossed the poultry/wild bird interface, with the viruses introduced to wintering birds. Overall, this study represented the largest AIV surveillance effort of wild birds within the Yangtze River wintering wetlands. Surveillance data highlighted the important role of wintering wild birds in the ecology of AIVs and may enable future early warnings of novel AIV emergence. [ABSTRACT FROM AUTHOR]

Field studies were conducted at Kunming (China) to determine the sensitivity of 23 wild sugarcane (Saccharum spontaneum L.) clones to enhanced ultraviolet-B radiation (UV-B, 280 to 315 nm). The clones sourced from different sites (altitude 0 to1860 m and latitude 18 to 37°N) were evaluated in the field during 2003 and 2004, both with and without supplemental UV-B radiation of 5.0 kJ m-2. Significant effects of UV-B radiation on plant height, leaf area index, number of tillers, and shoot biomass were observed, besides their significant inter-correlations. Based on the response index, it was deduced that intraspecific sensitivities of wild sugarcane clones to UV-B radiation are profound. Six most sensitive wild sugarcane clones had negative response index lower than -165 in 2003 and -110 in 2004, and six other most tolerant clones had positive response index higher than 150 in 2003 and 110 in 2004. Clones originating from regions with high ambient UV-B were not necessarily more tolerant to enhanced UV-B levels, implying the need to introduce tolerant strains from elsewhere to such locations. UV-B tolerant wild sugarcane clones identified and evaluated in this study might as well be useful as donors in sugarcane breeding programme. [ABSTRACT FROM AUTHOR]

Subtype H7 avian influenza viruses have been found to be associated with human infection and represent a risk for global public health. In 2013, the emergence of H7N9 virus in human beings and persistent human infection in China raised the most serious pandemic threat. Here we identified a human monoclonal antibody, P52E03, targeting the hemagglutinin (HA) of subtype H7 influenza viruses (H7 antigen), from a convalescent patient infected with H7N9 in 2017. P52E03 showed in vitro hemagglutination inhibiting (HI) and neutralizing activity against subtype H7 viruses belonging to both North American and Eurasian lineages. Moreover, it could prophylactically protect mice against weight loss and death caused by challenge with lethal H7N9 viruses in vivo and, therefore, is a candidate for development of antiviral agent against H7N9 infection. By generating escape mutant variants, we found that a single G151E substitution in the viral H7 antigenic site A could abort the neutralizing activity. Computational structural prediction of the P52E03/H7 complex revealed that residues including G151 in and around the conserved antigenic site A region are important for antigen recognition by the H7 cross-reactive antibody. Finally, we found that the P52E03 germline precursor (gHgL) antibody recognizes HA with measurable affinity, suggesting that its epitope is vulnerable to the human immune system and might elicit neutralizing antibodies (nAbs) in vivo after vaccination. • An H7-specific antibody P52E03 was generated from a natural H7N9 human infection. • P52E03 cross-reacted with divergent H7 viruses in Hemagglutination inhibition （HI） and microneutralization (MN) assays. • The epitope of P52E03 with a new key residue (G151) is conserved on H7 HA protein and vulnerable to the human immune system. • P52E03 confers protection against lethal H7N9 virus challenge in prophylactic and therapeutic settings in the mouse model. [ABSTRACT FROM AUTHOR]

The discovery of alphacoronaviruses and betacoronaviruses in plateau pikas ( Ochotona curzoniae ) expanded the host range of mammalian coronavirus (CoV) to a new order - Lagomorpha. However, the diversity and evolutionary relationships of CoVs in these plateau-region-specific animal population remains uncertain. We conducted a five-year longitudinal surveillance of CoVs harboured by pikas around Qinghai Lake, China. CoVs were identified in 33 of 236 plateau pikas and 2 of 6 Gansu pikas ( Ochotona cansus ), with a total positivity rate of 14.5%, and exhibiting a wide spatiotemporal distribution across seven sampling sites and six time points. Through meta-transcriptomic sequencing and RT-PCR, we recovered 16 near-complete viral genome sequences. Phylogenetic analyses classified the viruses as variants of either pika alphacoronaviruses or betacoronaviruses endemic to plateau pikas from the Qinghai-Tibet Plateau region. Of particular note, the pika-associated betacoronaviruses may represent a novel subgenus within the genus Betacoronavirus . Tissue tropism, evaluated using quantitative real-time PCR, revealed the presence of CoV in the rectal and/or lung tissues, with the highest viral loads at 10 3.55 or 10 2.80 RNA copies/μL. Surface plasmon resonance (SPR) assays indicated that the newly identified betacoronavirus did not bind to human or pika Angiotensin-converting enzyme 2 (ACE2) or Dipeptidyl peptidase 4 (DPP4). The findings highlight the ongoing circulation and broadening host spectrum of CoVs among pikas, emphasizing the necessity for further investigation to evaluate their potential public health risks.

In July 2022, an outbreak of highly pathogenic avian influenza A(H5N1) virus clade 2.3.4.4b occurred among migratory birds at Qinghai Lake in China. The virus circulated in June, and reassortants emerged after its introduction into the area. Surveillance in 2023 showed that the virus did not establish a stable presence in wild waterfowl.

Non-steroidal anti-inflammatory drugs-exacerbated respiratory disease （N-ERD） is a chronic respiratory disease characterized by eosinophilic inflammation, featuring chronic rhinosinusitis （CRS）, asthma, and intolerance to cyclooxygenase 1 （COX-1） inhibitors. The use of these medications can lead to an acute worsening of rhinitis and asthma symptoms. This condition has not yet received sufficient attention in China, with a high rate of misdiagnosis and a lack of related research. The Chinese Rhinology Research Group convened a group of leading young experts in otolaryngology from across the country, based on the latest domestic and international evidence-based medical practices to formulate this consensus.The consensus covers the epidemiology, pathogenesis, clinical manifestations, diagnostic methods, and treatment strategies for N-ERD, including pharmacotherapy, surgery, biologic treatments, and desensitization therapy. The goal is to improve recognition of N-ERD, reduce misdiagnosis, and enhance treatment outcomes., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Editorial Department of Journal of Clinical Otorhinolaryngology Head and Neck Surgery.)

Background: Acute respiratory infections in children are a global public health challenge. Owing to the coronavirus disease (COVID-19) pandemic, non-pharmaceutical interventions, including patient isolation, social distancing, hand washing, and mask wearing, have been widely implemented, impacting the transmission of common respiratory viruses. The aim of this study was to clarify the epidemiological features of respiratory viruses in children less than 14 years of age in Wuhan before and after COVID-19., Methods: Respiratory specimens were collected from patients aged < 14 years at two hospitals in Wuhan, China, from January 2018 to December 2021. Seven respiratory viruses were identified using an immunofluorescence assay. Pathogen profiles and seasonality were analysed., Results: The number of visits and virus detection rate decreased dramatically after February 2020. The respiratory virus detection rate peaked in January and December and decreased dramatically in February and August. The detection rate was lower in 2021 than in 2018 and 2019. Respiratory syncytial virus (RSV) was identified as the leading pathogen in children aged < 1 year and 1-4 years before and after the COVID-19 pandemic. In children aged 5-14 years, influenza virus was detected at the highest rate before, and RSV after, the COVID-19 pandemic. RSV was the most common virus in coinfections., Conclusions: This study revealed the epidemiological patterns of common respiratory viruses from 2018 to 2021. The spectrum of pathogens involved in paediatric respiratory infections had partly changed. Non-pharmaceutical interventions resulted in fewer opportunities for the spread of common viruses but also in an "immunity debt" that could have negative consequences when the pandemic is under control in Wuhan., (© 2023. The Author(s).)

Two novel reassortant highly pathogenic avian influenza viruses (H5N1) clade 2.3.4.4b.2 were identified in dead migratory birds in China in November 2021. The viruses probably evolved among wild birds through different flyways connecting Europe and Asia. Their low antigenic reaction to vaccine antiserum indicates high risks to poultry and to public health.

The source region of the Yellow River (SRYR) is an important water conservation and farming area in China. Under the dual influence of the natural environment and external pressure, ecological patches in the region are becoming increasingly fragmented, and landscape connectivity is continuously declining, which directly affect the landscape patch pattern and SRYR sustainable development. In the SRYR, morphological spatial pattern analysis (MSPA) and landscape index methods were used to extract ecologically important sources. Based on the minimum cumulative resistance model (MCR), Linkage Mapper was used to generate a potential corridor, and then potential stepped stone patches were identified and extracted by the gravity model and betweenness centrality to build an optimal SRYR ecological network. The distribution of patches in the core area of the SRYR was fragmented, accounting for 80.53% of the total grassland area. The 10 ecological sources based on the landscape connectivity index and 15 important corridors identified based on the MCR model were mainly distributed in the central and eastern regions of the SRYR. Through betweenness centrality, 10 stepped stone patches were added, and 45 planned ecological corridors were obtained to optimize the SRYR ecological network and enhance east and west connectivity. Our research results can provide an important reference for the protection of the SRYR ecosystem, and have important guiding significance and practical value for ecological network construction in ecologically fragmented areas.

The land use and ecological risk patterns in Guilin, which is the only innovation demonstration zone under the National Sustainable Development Agenda in China with a focus on the sustainable use of natural resources, have changed significantly as a result of the combined impact of climate change and human activities, thus presenting challenges to the sustainable development of the local area. This research employs an ecological risk assessment model and spatial analysis techniques in order to analyze the spatial correlation between land use and ecological risk, and to evaluate the spatial and temporal evolution characteristics of ecological risk at the overall and county scales in Guilin. The results reveal the following: (1) A total of 1848.6 km 2 land types in Guilin have changed from 2000 to 2020, and construction land has gradually expanded from the central urban area to the suburbs with increasing internal stability each year. (2) The ecological risk level in Guilin showed a decreasing trend at the city scale, but some regions still showed an increasing trend at the county distribution scale. (3) The ecological risk value in Guilin has significant spatial correlation, and the spatial distribution showed a clustering effect, which was consistent with the spatial distribution of ecological risk class areas. The research results can provide a reference for ecological risk control and sustainable development of landscape resource cities.