8 results on '"Carps microbiology"'
Search Results
2. Paracoccus luteus sp. nov., isolated from the intestine of grass carp.
- Author
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Ming H, Cheng LJ, Ding CL, Niu MM, Zhao ZL, Ji WL, Zhang LY, Zhang YM, Meng XL, and Nie GX
- Subjects
- Animals, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial genetics, Fatty Acids chemistry, Nucleic Acid Hybridization, Paracoccus isolation & purification, Phospholipids chemistry, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Carps microbiology, Intestines microbiology, Paracoccus classification, Phylogeny
- Abstract
A novel Gram-negative bacterium, designated CFH 10530
T , was isolated from the intestine of grass carp. The sample was collected from the aquaculture training base at the College of Fisheries, Henan Normal University, Xinxiang, PR China. Cells of strain CFH 10530T were coccoid, ovoid or short-rod-shaped, aerobic, non-spore-forming and non-motile. 16S rRNA gene sequence analysis demonstrated that strain CFH 10530T was closely related to Paracoccus endophyticus SYSUP0003T (97.7 % sequence similarity), Paracoccus halophilus HN-182T (96.5 %) and Paracoccus panacisoli DCY94T (96.1 %). The strain grew optimally at 25-28 °C, at pH 7.0 and with 0-2 % (w/v) NaCl. Cells were positive for catalase and oxidase, nitrate was reduced and H2 S was not produced. The isoprenoid quinone was Q-10. Major cellular fatty acids were summed feature 8, C18 : 0 and C18 : 0 3-OH. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, one unidentified aminolipid and five unidentified polar lipids. The genome size was 3 331 229 bp with a G+C content of 69.6 mol%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH 10530T and the other species of the genus Paracoccus were found to be below the recommended levels for species delineation (ANIm <85, ANIb <80 and dDDH <24 %). Based on its physiological properties, chemotaxonomic characteristics and low ANI and dDDH results, strain CFH 10530T is considered to represent a novel species for which the name Paracoccus luteus sp. nov., is proposed. The type strain is CFH 10530T (=KCTC 62919T =CGMCC 1.16597T ).- Published
- 2020
- Full Text
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3. A metabolomics and proteomics study of the Lactobacillus plantarum in the grass carp fermentation.
- Author
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Ming T, Han J, Li Y, Lu C, Qiu D, Li Y, Zhou J, and Su X
- Subjects
- Animals, China, Fermentation, Fish Products microbiology, Glycolysis, Lactobacillus plantarum genetics, Lactobacillus plantarum isolation & purification, Metabolomics, Proteomics, Carps microbiology, Fermented Foods microbiology, Lactobacillus plantarum metabolism
- Abstract
Background: Lactobacillus plantarum, a versatile lactic acid-fermenting bacterium, isolated from the traditional pickles in Ningbo of China, was chosen for grass carp fermentation, which could also improve the flavor of grass carp. We here explored the central metabolic pathways of L. plantarum by using metabolomic approach, and further proved the potential for metabolomics combined with proteomics approaches for the basic research on the changes of metabolites and the corresponding fermentation mechanism of L. plantarum fermentation., Results: This study provides a cellular material footprinting of more than 77 metabolites and 27 proteins in L. plantarum during the grass carp fermentation. Compared to control group, cells displayed higher levels of proteins associated with glycolysis and nucleotide synthesis, whereas increased levels of serine, ornithine, aspartic acid, 2-piperidinecarboxylic acid, and fumarate, along with decreased levels of alanine, glycine, threonine, tryptophan, and lysine., Conclusions: Our results may provide a deeper understanding of L. plantarum fermentation mechanism based on metabolomics and proteomic analysis and facilitate future investigations into the characterization of L. plantarum during the grass carp fermentation.
- Published
- 2018
- Full Text
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4. Effects of dietary microencapsulated sodium butyrate on growth, intestinal mucosal morphology, immune response and adhesive bacteria in juvenile common carp (Cyprinus carpio) pre-fed with or without oxidised oil.
- Author
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Liu W, Yang Y, Zhang J, Gatlin DM, Ringø E, and Zhou Z
- Subjects
- Animals, Antioxidants chemistry, Aquaculture, Butyric Acid chemistry, Carps immunology, Carps metabolism, Carps microbiology, China, Cytokines genetics, Cytokines metabolism, Fish Proteins genetics, Fish Proteins metabolism, Gene Expression Regulation, Developmental, Gram-Negative Bacteria growth & development, Gram-Negative Bacteria immunology, Gram-Negative Bacteria isolation & purification, Gram-Positive Bacteria growth & development, Gram-Positive Bacteria immunology, Gram-Positive Bacteria isolation & purification, HSP70 Heat-Shock Proteins genetics, HSP70 Heat-Shock Proteins metabolism, Intestinal Mucosa immunology, Intestinal Mucosa microbiology, Intestinal Mucosa ultrastructure, Intestines growth & development, Intestines immunology, Intestines microbiology, Intestines ultrastructure, Microvilli immunology, Microvilli microbiology, Microvilli ultrastructure, Oxidation-Reduction, Oxidative Stress, Solubility, Soybean Oil adverse effects, Soybean Oil antagonists & inhibitors, Soybean Oil chemistry, Antioxidants metabolism, Bacterial Adhesion, Butyric Acid metabolism, Carps growth & development, Diet veterinary, Immunity, Mucosal, Intestinal Mucosa growth & development
- Abstract
The aim of the present study was to investigate the effects of different dietary sustained-release microencapsulated sodium butyrate (MSB) products (0 (non-supplement), 1·5 and 3·0 h) for a control or oxidised soyabean oil (SBO) diet on fish production, intestinal mucosal condition, immunity and intestinal bacteria in juvenile common carp (Cyprinus carpio). Dietary MSB increased weight gain and reduced the feed conversion ratio within the control and oxidised SBO groups. Gut mucosa was damaged in the oxidised SBO group fed without MSB, in contrast to a normal appearance found in fish fed the MSB1·5 and MSB3·0 diets in the oxidised SBO group. Microvillus density increased in fish fed the MSB1·5 and MSB3·0 diets in the oxidised SBO group (P< 0·001); however, microvillus density was affected by the different pre-fed diets in the midgut (P< 0·001) and by the different sustained-release times of MSB in the distal gut (DG) (P= 0·003). The interaction between the pre-fed diets and the sustained-release times of dietary MSB was significant for the relative gene expression levels of gut heat shock protein-70 (HSP70), pro-inflammatory cytokines (IL-1β and TNF-α) and anti-inflammatory cytokines (transforming growth factor-β) within each gut segment, except for HSP70 in the DG and IL-1β in the foregut. Modulation of adherent bacterial communities within each gut segment investigated was not obvious when the common carp were fed the diets with MSB, as similarity coefficients of >0·79 were observed. These results indicated that MSB can be used as a dietary supplement to repair or prevent intestinal damage in carp fed oxidised SBO.
- Published
- 2014
- Full Text
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5. Proteomic analysis of hepatic tissue of Cyprinus carpio L. exposed to cyanobacterial blooms in Lake Taihu, China.
- Author
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Jiang J, Wang X, Shan Z, Yang L, Zhou J, and Bu Y
- Subjects
- Aldehyde Dehydrogenase metabolism, Animals, Bacterial Toxins toxicity, Carps metabolism, China, Cyanobacteria Toxins, Electron Spin Resonance Spectroscopy, Electrophoresis, Gel, Two-Dimensional, Fishes, Geography, Glucose metabolism, Hydrogen-Ion Concentration, Lakes, Liver microbiology, Marine Toxins toxicity, Mass Spectrometry, Microcystins toxicity, Phosphoprotein Phosphatases metabolism, Reactive Oxygen Species metabolism, Temperature, Water Microbiology, Carps microbiology, Cyanobacteria, Eutrophication, Liver metabolism, Proteome metabolism
- Abstract
With the rapid development of industry and agriculture and associated pollution, the cyanobacterial blooms in Lake Taihu have become a major threat to aquatic wildlife and human health. In this study, the ecotoxicological effects of cyanobacterial blooms on cage-cultured carp (Cyprinus carpio L.) in Meiliang Bay of Lake Taihu were investigated. Microcystins (MCs), major cyanobacterial toxins, have been detected in carp cultured at different experimental sites of Meiliang Bay. We observed that the accumulation of MCs in carp was closely associated with several environmental factors, including temperature, pH value, and density of cyanobacterial blooms. The proteomic profile of carp liver exposed to cyanobacterial blooms was analyzed using two-dimensional difference in-gel electrophoresis (2D-DIGE) and mass spectrometry. The toxic effects of cyanobacterial blooms on carp liver were similar to changes caused by MCs. MCs were transported into liver cells and induced the excessive production of reactive oxygen species (ROS). MCs and ROS inhibited protein phosphatase and aldehyde dehydrogenase (ALDH), directly or indirectly resulting in oxidative stress and disruption of the cytoskeleton. These effects further interfered with metabolic pathways in the liver through the regulation of series of related proteins. The results of this study indicated that cyanobacterial blooms pose a major threat to aquatic wildlife in Meiliang Bay in Lake Taihu. These results provided evidence of the molecular mechanisms underlying liver damage in carp exposed to cyanobacterial blooms.
- Published
- 2014
- Full Text
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6. Nephrotoxic effects from chronic toxic cyanobacterial blooms in fishes with different trophic levels in a large Chinese lake.
- Author
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Qiu T, Xie P, Li L, Guo L, Zhang D, and Zhou Q
- Subjects
- Animals, Biomarkers metabolism, Carps microbiology, Catalase metabolism, China, Cyanobacteria growth & development, Environmental Monitoring methods, Fish Diseases metabolism, Fish Diseases pathology, Fish Proteins metabolism, Fishes metabolism, Glutathione metabolism, Glutathione Peroxidase metabolism, Glutathione Transferase metabolism, Goldfish microbiology, Humans, Kidney metabolism, Kidney ultrastructure, Kidney Diseases metabolism, Kidney Diseases microbiology, Kidney Diseases pathology, Liver Failure microbiology, Liver Failure veterinary, Multivariate Analysis, Risk Assessment, Superoxide Dismutase metabolism, Time Factors, Cyanobacteria pathogenicity, Fish Diseases microbiology, Fishes microbiology, Fresh Water microbiology, Harmful Algal Bloom, Kidney microbiology, Kidney Diseases veterinary, Water Microbiology
- Abstract
Physiological and biochemical responses in kidneys of fishes with different trophic levels to toxic cyanobacterial blooms were studied. We sampled four fishes: the phytoplanktivorous Hypophthalmichthys molitrix and Aristichthys nobilis, the omnivorous Carassius auratus, and the carnivorous Culter ilishaeformis. Alterations of the antioxidant (GSH) and the major antioxidant enzymes (CAT, SOD, GPx, GST) were monitored monthly. Catalase and glutathione S-transferase were significantly higher during blooms than before and after blooms. All fishes showed ultrastructural alterations during blooms, which mainly are inosculation of foot processes in epithelial cell of glomeruli and mitochondria swelling in the proximal tubules. The results suggested that kidney impairment from chronic exposure of toxic cyanobacterial blooms might be the first step, and then followed by hepatic failure. Compared with livers in terms of physiological status, the weaker antioxidant ability of kidney made it more susceptible to chronic MCs exposure, besides its effective accumulation of MC metabolites., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2012
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7. Immunomodulatory effects of secondary metabolites from thermophilic Anoxybacillus kamchatkensis XA-1 on carp, Cyprinus carpio.
- Author
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Wang GX, Wang Y, Wu ZF, Jiang HF, Dong RQ, Li FY, and Liu XL
- Subjects
- Aeromonas hydrophila immunology, Animals, Anoxybacillus genetics, China, Dipeptides analysis, Immunomodulation drug effects, Interleukin-1beta metabolism, Magnetic Resonance Spectroscopy, Mass Spectrometry, Muramidase blood, Nitric Oxide Synthase Type II metabolism, Phagocytosis, RNA, Ribosomal, 16S genetics, Spectrophotometry, Infrared, Superoxide Dismutase blood, Survival Analysis, Anoxybacillus chemistry, Carps immunology, Carps microbiology, Dipeptides immunology, Dipeptides pharmacology, Immunomodulation immunology
- Abstract
A bacterial strain with putative immunomodulatory properties was isolated from Xi'an hot springs in China. Comparison of 16S rRNA gene revealed a 97% similarity between the tested strain (designated XA-1) and Anoxybacillus kamchatkensis. Two compounds isolated from the secondary metabolites of XA-1 were identified by spectral data (infrared, nuclear magnetic resonance and mass spectrometry) as: (1) cyclo (Gly-L-Pro) and (2) cyclo (L-Ala-4-hydroxyl-L-Pro). Two cyclic dipeptides showed stimulatory properties towards a range of parameters when a dose of 20mg kg(-1) body weight was intraperitoneally injected in naive common carp, Cyprinus carpio. Innate immune parameters (serum SOD, lysozyme and bactericidal activity, and phagocytic activity by peripheral blood leucocytes) along with the expression of two immune-related genes (IL-1β and iNOS) in blood were examined after 7, 14, 21, and 28 days of injection. In the absence of infection, immunomodulators should ideally not affect normal physiology and immunity of the host; possible negative outcomes of activated immune responses in the naive state are discussed. Protection by two bacterial dipeptides was assessed in an intraperitoneal injection challenge trial with live Aeromonas hydrophila. Both compounds reduced mortality, with the highest survival rate observed in the group that received compound 2 (80%) followed by the group that received compound 1 (65%) while control group scored the worse (15%). Elucidation of the involved protective mechanisms in carp requires future studies., (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Published
- 2011
- Full Text
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8. Clostridium perfringens toxin types from freshwater fishes in one water reservoir of Shandong Province of China, determined by PCR.
- Author
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Cai Y, Gao J, Wang X, Chai T, Zhang X, Duan H, Jiang S, Zucker BA, and Schlenker G
- Subjects
- Animals, Carps microbiology, Catfishes microbiology, China epidemiology, Clostridium Infections epidemiology, Clostridium Infections microbiology, Disease Reservoirs veterinary, Fish Diseases epidemiology, Food Contamination prevention & control, Fresh Water microbiology, Polymerase Chain Reaction methods, Species Specificity, Water Microbiology, Bacterial Toxins isolation & purification, Clostridium Infections veterinary, Clostridium perfringens isolation & purification, Fish Diseases microbiology, Polymerase Chain Reaction veterinary
- Abstract
Four hundred and twenty intestinal content samples (not including intestinal tissues) of freshwater fishes (60 silver carps, 100 carps, 100 crucian carps, 60 catfishes and 100 zaieuws) caught from one water reservoir were examined bacteriologically for the occurrence of C. perfringens. Isolates were examined by polymerase chain reaction (PCR) for genes encoding the four lethal toxins (alpha, beta, epsilon and iota) for classification into toxin types and for genes encoding enterotoxin and the novel beta2 toxin for further subclassification. C. perfringens could be isolated in 75 intestinal contents samples (17.9%) from freshwater fish including: 13 silver carps, 2 carps, 12 crucian carps, 40 zaieuws, and 8 catfishes. In 75 isolates, 58 strains (77.3%) were C. perfringens toxin type C (alpha and beta toxin positive), 13 strains (17.3%) were toxin type A (alpha toxin positive) and 4 strains (5.3%) were toxin type B (alpha, beta and epsilon toxin positive). In addition, the gene encoding for beta2 toxin was found in 47 strains (62.7%) of all the isolates, seven from type A, two from type B, and 38 from type C. The gene encoding for enterotoxin was not found in any isolate. These amplified toxin gene fragment were cloned and sequenced and compared with reference strains, the identity varied from 98.15% to 99.29%. This is the first report of C. perfringens alpha, beta, epsilon, beta2 toxins in freshwater fish and of beta, epsilon toxins in fish in general, and is the first discovery that the beta2 toxin could be detected in strains of type B. The origin of this bacterium and its importance to human food poisoning in freshwater fish is discussed.
- Published
- 2008
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