1. Phylogenetic Tree Analysis of First Psychrobacter Sp. Strain From Blood of Iraqi Patient; A Case Report.
- Author
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Jassim, Nuha S., Ameer Alash, Sameer Abdul, and Ahmed, Najwa Shihab
- Subjects
GRAM-negative bacteria ,GRAM'S stain ,BLOODSTAINS ,OLDER women ,PRESSURE ulcers - Abstract
Psychrobacter spp. are a Gram negative bacteria, aerobic, non-motile, small, with coccobacilli shape. Originally isolated from seaweed samples and marine environments. Recently considered as rare opportunistic human pathogens. Sixty -five years old women admitted to hospital with diabetic mellitus and stage 4 pressure ulcers (PU) with seizure and mild fever 37.9 °C. A gram staining of blood culture revealed gram negative bacteria have a cocobacilli shape. The VITEK2 system (bioMérieux) misidentify the isolate as Acinetobacter bumannii complex with low discrimination. The submission of the bacterial isolate to the GenBank BLAST search tool revealed that the Iraqi isolate show 100% homology with Psychrobacter sp. From china with accession number ID: MK205167.1, the next matches with Uncultured Psychrobacter sp. (ID: KF859544.1 China) Psychrobacter pulmonis(ID: KU364058.1, India), Psychrobacter pulmonis (ID: MH550129.1, China)with 99% similarity for each one. This Psychrobacter sp. was the first isolate from bacteremia patients in Iraq. The identification based on 16S rRNA gene sequence for precisely identify this bacteria that misidentified by VITEK2 system. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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