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Background: Brucella canis is the etiological agent of canine brucellosis, a worldwide neglected zoonosis that constitutes one of the major infectious causes of infertility and reproductive failure in dogs. Although genomic information available for this pathogen has increased in recent years, here we report the first genome sequencing of a B. canis strain in Chile, and the differences in virulence genes with other B. canis strains. Results: Genome assembly produced a total length of 3,289,216 bp, N50 of 95,163 and GC% of 57.27, organized in 54 contigs in chromosome I, and 21 contigs in chromosome II. The genome annotation identified a total of 1981 CDS, 3 rRNA and 36 tRNA in chromosome I, and 1113 CDS and 10 tRNA in chromosome II. There is little variation between the different strains and the SCL isolate. Phylogenetic analysis showed that the Chilean SCL strain is closely related to B. canis and B. suis strains. Small differences were found when compared to the Serbian isolate, but all strains shared the same recent common ancestor. Finally, changes in the sequence of some virulence factors showed that the SCL strain is similar to other South American B. canis strains. Conclusions: This work sequenced and characterized the complete genome of B. canis strain SCL, evidencing the complete presence of all the genes of the virB operon, and minor changes in outer membrane proteins and in the urease operon. Our data suggest that B. canis was introduced from North America and then spread throughout the South American continent. [ABSTRACT FROM AUTHOR]

Simple Summary: Wild canids play a crucial role in the environment, being an ecological agent of flora dispersal, top predators, and environmental sentinels for zoonotic emerging pathogens, such as Brucella spp. and pathogenic Leptospira. In Chile, three species of non-domestic native canids are present, and due to the growing concern about their interaction with humans and domestic animals, it is of utmost relevance to determine the role of these animals in the epidemiology of brucellosis and leptospirosis. This study aimed to detect the exposure to B. abortus, B. canis, and pathogenic Leptospira by serologic, bacteriologic, and molecular techniques in native foxes from rehabilitation and exhibition centers in Central Chile. Forty-six blood samples were obtained from culpeo and grey foxes, detecting exposure to B. canis and L. Javanica in 10.9% and 7.7%, respectively. Exposure was not registered by culture and qPCR in any of the sampled animals. Our results show for the first time in Chile exposure to B. canis in native foxes, highlighting the need to establish integrated surveillance programs to better evaluate the role of wild animals in the epidemiology of emerging zoonotic pathogens that may affect One Health. Brucella abortus, B. canis, and pathogenic Leptospira are zoonotic pathogens that infect humans, as well as domestic and wild animals. In wild canids, they may affect their fertility and reproduction, threatening their conservation. Wild canids play a crucial role in the environment as meso- and top-predators and environmental sentinels for zoonotic pathogens. In Chile, three species of wild canids are present, and due to changes in land use and environmental dynamics, it is of utmost relevance to determine the role of these species in the epidemiology of brucellosis and leptospirosis. This study aimed to detect the exposure to B. abortus, B. canis, and pathogenic Leptospira by serologic, bacteriologic, and molecular techniques in native foxes from rehabilitation and exhibition centers in Central Chile. Forty-six blood samples were obtained from Lycalopex culpaeus and L. griseus, detecting 10.9% of seropositivity to B. canis and 7.7% to L. Javanica. No seropositivity was seen for B. abortus. Exposure was not registered by culture and qPCR in any of the sampled animals. Our findings are the first register of exposure to any Brucella species in wild canids in Chile and highlight the need to establish surveillance programs of these emerging pathogens. [ABSTRACT FROM AUTHOR]

Simple Summary: Canine brucellosis caused by Brucella canis is a zoonotic disease that is considered the main infectious cause of infertility and reproductive failure in dogs worldwide, particularly in Latin America. Reports of B. canis infection in people have increased, especially in people who work with dogs and their owners, where the adoption of stray dogs poses a public health risk. Thus, this study determined the prevalence of infection in kennels, household, and stray dogs in the Metropolitan region, Chile, recording the genomic characteristics of the isolated circulating strains. Our results demonstrate that the infection is widespread in the three canine populations analyzed, with no differences in age or sex, and most of the infected animals do not show clinical signs or bacteremia. Furthermore, the high genetic similarity of the isolated strains suggests a common transmission route throughout the country. This study corroborates the need to implement official strategies for the control and prevention of B. canis infection, including sanitary, diagnostic, and educational measures under the One Health vision. Canine brucellosis caused by Brucella canis is a zoonotic disease that causes reproductive alterations in dogs, such as infertility, abortion, and epididymitis. This pathogen is especially prevalent in South America, and due to the lack of official control programs and the growing trend of adopting dogs it constitutes a public health risk that must be addressed. The aim of this study was to determine the prevalence of B. canis infection in kennel, shelter, and household dogs and to characterize the genomic properties of circulating strains, including ure and virB operons and omp25/31 genes. Samples from 771 dogs were obtained, and the infection was detected by blood culture and/or serology in 7.0% of the animals. The complete ure and virB operons and the omp25/31 genes were detected. Interestingly, we found different single-nucleotide polymorphisms (SNPs) in some of the analyzed genes, which could mean a change in the fitness or virulence of these strains. This study provides further evidence about dogs as a source of B. canis strains that can infect people. This also highlights the need to implement official control programs, including the mandatory testing of dogs, especially stray dogs, before adoption. [ABSTRACT FROM AUTHOR]

We investigated exposure to Brucella canis and Leptospira spp. in sera from 56 canids sampled between 2008 and 2012 in Tierra del Fuego, Chile. No seropositives to B. canis were found. We detected antibodies against Leptospira spp. in Fuegian culpeo fox ( Pseudalopex culpaeus lycoides ; 20%), chilla foxes ( Pseudalopex griseus ; 8%), and dogs ( Canis lupus familiaris ; 3%).