1. Molecular and experimental evidence refuse the life cycle of Proctoeces lintoni (Fellodistomidae) in Chile.
- Author
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Oliva ME, Valdivia IM, Cárdenas L, George-Nascimento M, Gonzalez K, Guiñez RE, and Cuello D
- Subjects
- Animals, Chile, DNA, Helminth chemistry, DNA, Helminth genetics, DNA, Ribosomal chemistry, DNA, Ribosomal genetics, DNA, Ribosomal Spacer chemistry, DNA, Ribosomal Spacer genetics, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 18S genetics, Sequence Analysis, DNA, Trematoda classification, Trematoda genetics, Bivalvia parasitology, Life Cycle Stages, Trematoda isolation & purification, Trematoda physiology
- Abstract
The mussel Perumitylus purpuratus has been described as the first intermediate host for the digenea Proctoeces lintoni (Fellodistomidae) in the Chilean coast. The study of more than 3000 specimens of P. purpuratus, taken off northern Chile revealed the absence of sporocysts. Experimental infection of mussels with eggs obtained from the known host for P. lintoni was unsuccessful. We analyze the V4 region of the SSU rRNA of living sporocysts and cercariae obtained from P. purpuratus from central Chile in order to confirm the proposed life cycle for this digenea. Our results demonstrated that sporocysts and cercariae obtained from P. purpuratus do not belong to P. lintoni but to an undescribed digenea from the Chilean coast.
- Published
- 2010
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