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In this study, we investigated the physiological and biochemical responses of Ulva species to variation in nutrient availability. Sampling was conducted at two sites on Jeju Island, Korea, namely, Handong, which is close to seven intensive land-based fish farms, and Hado, which has no apparent nearby nutrient sources. We examined the water column nutrient concentrations, nitrate reductase (NR) activity, nitrate uptake efficiency, tissue C, N, and P content, and stable isotope ratios of Ulva species. Water column NH4+, NO3− + NO2−, and PO43− concentrations were significantly higher at Handong than at Hado. NR activity and tissue N content of Ulva species were significantly higher at Handong than at Hado. Notably, nitrate uptake efficiency was inversely proportional to NR activity and tissue N content. The physiological and biochemical responses of Ulva species were closely related to dissolved inorganic nitrogen, which stimulates Ulva species to regulate growth. Additionally, the δ15N values of Ulva tissues at both sites were within the previously reported range for fresh groundwater. Therefore, the main nitrogen source for Ulva growth may be submerged groundwater with high nutrient concentrations. Our results provide invaluable information for estimating dissolved inorganic nitrogen levels in water, which may facilitate development of management policies. [ABSTRACT FROM AUTHOR]