1. Comparison of different IRT-PAP protocols to screen newborns for cystic fibrosis in three central European populations.
- Author
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Sommerburg, Olaf, Krulisova, Veronika, Hammermann, Jutta, Lindner, Martin, Stahl, Mirjam, Muckenthaler, Martina, Kohlmueller, Dirk, Happich, Margit, Kulozik, Andreas E., Votava, Felix, Balascakova, Miroslava, Skalicka, Veronika, Stopsack, Marina, Gahr, Manfred, Macek, Milan, Mall, Marcus A., and Hoffmann, Georg F.
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CYSTIC fibrosis , *NEONATAL diseases , *PANCREATITIS , *SENSITIVITY analysis - Abstract
Abstract: Background: In recent years different IRT/PAP protocols have been evaluated, but the individual performance remains unclear. To optimize the IRT/PAP strategy we compared protocols from three regional CF newborn screening centers (Heidelberg, Dresden, and Prague). Methods: We evaluated the effect of elevating the IRT-cut-off from 50 to 65μg/l (~97.5th to ~99.0th percentile), the need of a failsafe protocol (FS, IRT≥99.9th percentile) and the relative performance using either two IRT-dependent PAP-cut-offs or one PAP-cut-off. Findings: Elevation of the IRT cut-off to 65μg/l (~99.0th percentile) increased the PPV significantly (Dresden: 0.065 vs. 0.080, p<0.0001, Prague: 0.052 vs. 0.074, p<0.0001) without reducing sensitivity. All three IRT/PAP protocols showed a trend towards a higher sensitivity with FS than without and when using one PAP-cut-off instead of two IRT-dependent PAP-cut-offs. Conclusions: For best performance we suggest an IRT/PAP protocol with an IRT-cut-off close to the 99.0th percentile, FS, and a single PAP-cut-off. [Copyright &y& Elsevier]
- Published
- 2014
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