1. Isolation of verotoxin-producing Escherichia coli O-rough:K1:H7 from two patients with traveler's diarrhea.
- Author
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Vila J, Vargas M, Ruiz J, Gallardo F, Jimenez de Anta MT, and Gascón J
- Subjects
- Adult, Anti-Bacterial Agents pharmacology, Bacteriological Techniques, Central America epidemiology, DNA, Bacterial analysis, Diarrhea epidemiology, Electrophoresis, Gel, Pulsed-Field, Escherichia coli drug effects, Escherichia coli Infections epidemiology, Feces microbiology, Female, Genome, Bacterial, Humans, India epidemiology, Male, Microbial Sensitivity Tests, O Antigens immunology, O Antigens metabolism, Polymerase Chain Reaction methods, Polymorphism, Restriction Fragment Length, Shiga Toxin 1, Travel, Bacterial Toxins metabolism, Diarrhea microbiology, Escherichia coli isolation & purification, Escherichia coli metabolism, Escherichia coli Infections diagnosis, Escherichia coli Infections microbiology
- Abstract
Two Escherichia coli O-rough:K1:H7 strains producing verotoxin 1 that were isolated from stool samples of two travelers with diarrhea who consulted our clinic after trips to the Indian Subcontinent and Central America were characterized. Both strains were sorbitol negative, the same phenotype presented by E. coli O157:H7, but in contrast they were beta-glucuronidase positive. Low-frequency restriction analysis of chromosomal DNA and pulsed-field gel electrophoresis and repetitive extragenic palindrome-PCR showed that both strains were epidemiologically related. The illness was self-limited in both cases but involved long-duration, watery diarrhea (10 to 50 days) accompanied by abdominal cramps and flatulence. This serotype should be taken into account as a possible cause of traveler's diarrhea.
- Published
- 1997
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