Search

The mining industry often must mix different kinds of water on the mine site during pre-treatment or post-treatment before the final discharge of the treated water to the environment. Microbubble ozonation has proven to be efficient in the removal of contaminants of concern from mine water, such as metals, metalloids, and nitrogen compounds, which can persist in the environment and entail toxicity issues. This study evaluated the efficiency of ozone microbubbles combined with lime precipitation on contaminant removal and its impact on toxicity for Daphnia magna with five different mine effluent mixes from an active mine site located in Abitibi-Témiscamingue, QC, Canada. For the non-acidic mixes, two scenarios were tested: first, pre-treatment of metals using lime precipitation and a flocculant was conducted prior to ozonation; and second, ozonation was conducted prior to metals post-treatment using the same precipitation and flocculation technique. Results showed that the NH 3 –N removal efficiency ranged from 90% for the lower initial concentrations (1.1 mg/L) to more than 99% for the higher initial concentrations (58.4 mg/L). Moreover, ozonation without metals pre-treatment improved NH 3 –N treatment efficiency in terms of kinetics but entailed abnormal toxicity issues. Results of bioassays conducted on water with metals pre-treatment did not show any toxicity events but showed abnormal toxicity patterns on the mixes treated without metals pre-treatment (diluted effluents were toxic, while undiluted were not). At 50% dilution, the water was toxic, probably due to the potential presence of metal oxide nanoparticles. The confirmation of the source of toxicity requires further investigation. [Display omitted] • Mines often must mix different types of water before the final discharge. • Ozone microbubbles can reduce the concentration of contaminants complex to treat. • Metal pre-treatment is necessary while using ozone microbubble process. • Ozone treatment can produce metal oxide nanoparticles in presence of metals. • Daphnia magna is sensitive to metal oxide nanoparticles at low hardness. [ABSTRACT FROM AUTHOR]

Given the ability of engineered metal nanoparticles to be transformed in natural waters in unpredictable manners, various sampling methods must be developed. Here, we took a novel approach to collection silver nanoparticles (AgNPs) that involved the use of the intact periostracum, the outer proteinaceous organic layer, of freshwater unionid mussels Pyganodon sp. Eight adult mussels were collected in August 2019 from a small boreal lake (L222) at the International Institute for Sustainable Development - Experimental Lakes Area (northwestern Ontario), which had been dosed with 15 kg of poly(vinylpyrrolidone)-coated silver nanoparticles (PVP-AgNPs) in 2014–2015. Additionally, three adult mussels were collected from a control lake (L375). Numerous silica (SiO 2) diatom frustules were adhered to periostracum of all mussels. Intact periostracum promotes the formation of layer composed of diatoms and sand grains. The Ag content in soft tissues and shells of the mussels from L375 was as low as ≤ 0.1 μg/g. In mussels from L222, Ag concentrations in the periostracum of five shells were in detectable amounts (1–4 μg/g); in three shells concentrations were as high as 86, 122, and 494 μg/g. The underlying mineral shell is depleted in Ag (<0.1 μg/g). The Ag content in soft tissue organs (whole body) ranged from 44 to 191 μg/g. AgNPs occur on the surface of both periostracum and diatoms. Single AgNPs (d = 20–60 nm) were partly sulfidized to Ag 2 S. The observed AgNPs often form aggregates with an average and a maximal size of circa 100 nm and 1.5 μm, respectively. Scraping small fragments of intact periostracum of unionid shell is non-lethal to mussels, and is easy to do under field conditions. This simple sampling protocol could be used to detect metal-based nanoparticles (engineered or accidental) with the use of unionid and dreissenid bivalves. [Display omitted] • We studied shells of bivalves collected from a lake dosed with Ag nanoparticles. • Numerous silica diatom frustules were found adhered to shell periostracum. • AgNPs occur on the surface of both shell periostracum and diatoms. • NPs often form aggregates. • Single AgNPs are partly sulfidized to Ag 2 S NPs. [ABSTRACT FROM AUTHOR]

The occurrence of silver (Ag) in urban effluents is partly associated with the increasing use of silver nanoparticles (Ag NPs) as an antiseptic agent in various consumer products. Distinction among Ag forms must be taken into account in the assessment of exposure and toxicological effects to aquatic organisms. Wastewater treatment processes effectively remove Ag particles and colloids (mostly > 95%), but this still leaves notable concentrations (in order of ng/L) escaping to effluent-receiving waters. Total suspended Ag concentrations in various studied effluents ranged from 0.1 to 6 ng/L. The purpose of this study was then to measure and characterize Ag NPs in urban effluents for their concentrations and size distribution using the single particle ICP-MS technique (SP-ICP-MS). Wastewater influents and effluents from various treatment plants-from aerated lagoons to advanced treatment technology-were collected for three sampling days. Our results showed the presence of Ag NP in all samples with concentrations reaching 0.5 ng/L on a mass basis. However, on a particle number basis, Ag NP concentrations (expressed in particle/mL) in the 20-34-nm fraction (up to 3400 particles/mL) were much more abundant (> 700%) than in the > 35-nm larger fraction. The proportion of Ag at the nanoscale (1-100 nm) represents less than 8% of the total suspended Ag for all effluent samples, regardless of their origins. A significant correlation (linear regression: r 2 > 0.7) was observed between Ag NP and total suspended Ag concentrations in investigated effluents. Because Ag nanotoxicity is size dependent, the determination of size distribution and exposure concentration on a particle number basis is urgently needed for risk assessment of this class of nanoparticles., (© 2021. Crown.)

A total of 15 kg of silver nanoparticles (AgNPs) was added continuously over two ice-free field seasons to a boreal lake (i.e., Lake 222) at the IISD Experimental Lakes Area in Canada. We monitored the accumulation of silver (Ag) in the tissues of yellow perch ( Perca flavescens) and northern pike ( Esox lucius) exposed to the AgNPs under environmentally relevant conditions. The greatest accumulation was observed in the liver tissues of pike, and a single pike sampled in the second year of additions had the highest concentration observed in liver of 5.1 micrograms per gram of wet weight. However, the Ag concentrations in gill and muscle tissue of both pike and perch did not exceed 0.35 micrograms per gram of wet weight. Following additions of AgNP, the Ag residues in fish tissues declined, with a half-life of Ag in pike liver of 119 days. Monitoring using passive sampling devices and single-particle inductively coupled plasma mass spectrometry during the AgNP addition phase confirmed that Ag nanoparticles were present in the water column and that estimated mean concentrations of Ag increased over time to a maximum of 11.5 μg/L. These data indicate that both a forage fish and a piscivorous fish accumulated Ag in a natural lake ecosystem dosed with AgNPs, leading to Ag concentrations in some tissues of the piscivorous species that were 3 orders of magnitude greater than the concentrations in the water.