25 results on '"Johnson R"'
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2. Detection and Prevalence of Verotoxin-Producing Escherichia coli O157 and Non-O157 Serotypes in a Canadian Watershed.
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Johnson, R. P., Holtslander, B., Mazzocco, A., Roche, S., Thomas, J. L., Pollari, F., and Pintar, K. D. M.
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ESCHERICHIA coli , *SEROTYPES , *WATERSHEDS , *IMMUNOMAGNETIC separation , *POPULATION - Abstract
Verotoxin-producing Escherichia coli (VTEC) strains are the cause of food-borne and waterborne illnesses around the world. Traditionally, surveillance of the human population as well as the environment has focused on the detection of E. coli O157:H7. Recently, increasing recognition of non-O157 VTEC strains as human pathogens and the German O104:H4 food-borne outbreak have illustrated the importance of considering the broader group of VTEC organisms from a public health perspective. This study presents the results of a comparison of three methods for the detection of VTEC in surface water, highlighting the efficacy of a direct VT immunoblotting method without broth enrichment for detection and isolation of O157 and non-O157 VTEC strains. The direct immunoblot method eliminates the need for an enrichment step or the use of immunomagnetic separation. This method was developed after 4 years of detecting low frequencies (1%) of E. coli O157:H7 in surface water in a Canadian watershed, situated within one of the FoodNet Canada integrated surveillance sites. By the direct immunoblot method, VTEC prevalence estimates ranged from 11 to 35% for this watershed, and E. coli O157:H7 prevalence increased to 4% (due to improved method sensitivity). This direct testing method provides an efficient means to enhance our understanding of the prevalence and types of VTEC in the environment. This study employed a rapid evidence assessment (REA) approach to frame the watershed findings with watershed E. coli O157:H7 prevalences reported in the literature since 1990 and the knowledge gap with respect to VTEC detection in surface waters. [ABSTRACT FROM AUTHOR]
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- 2014
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3. Sockeye salmon () return after an absence of nearly 90 years: a case of reversion to anadromy.
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Godbout, L., Wood, C.C., Withler, R.E., Latham, S., Nelson, R.J., Wetzel, L., Barnett-Johnson, R., Grove, M.J., Schmitt, A.K., McKeegan, K.D., and Bradford, Michael
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SOCKEYE salmon ,MITOCHONDRIAL DNA - Abstract
Copyright of Canadian Journal of Fisheries & Aquatic Sciences is the property of Canadian Science Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2011
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4. Airborne observation of aerosol optical depth during ARCTAS: vertical profiles, inter-comparison and fine-mode fraction.
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Shinozuka, Y., Redemann, J., Livingston, J. M., Russell, P. B., Clarke, A. D., Howell, S. G., Freitag, S., O'Neill, N. T., Reid, E. A., Johnson, R., Ramachandran, S., McNaughton, C. S., Kapustin, V. N., Brekhovskikh, V., Holben, B. N., and McArthur, L. J. B.
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AEROSOLS ,SMOKE plumes ,BIOMASS burning ,CARBON-black ,ATTENUATION of light ,MASS spectrometry - Abstract
We describe aerosol optical depth (AOD) measured during the Arctic Research of the Composition of the Troposphere from Aircraft and Satellites (ARCTAS) experiment, focusing on vertical profiles, inter-comparison with correlative observations and fine-mode fraction. Arctic haze observed in <2 km and 2-4 km over Alaska in April 2008 originated mainly from anthropogenic emission and biomass burning, respectively, according to aerosol mass spectrometry and black carbon incandescence measurements. The Angstrom exponent for these air masses is 1.4 ± 0.3 and 1.7 ± 0.1, respectively, when derived at 499 nm from a second-order polynomial fit to the AOD spectra measured with the 14-channel Ames Airborne Tracking Sunphotometer (AATS-14) over 354-2139 nm. We examine 55 vertical profiles selected from all phases of the experiment. For two thirds of them, the AOD spectra are within 3% + 0.02 of the vertical integral of local visible-light scattering and absorption. The horizontal structure of smoke plumes from local biomass burning observed in central Canada in June and July 2008 explains most outliers. The differences in mid-visible Angstrom exponent are <0.10 for 63% of the profiles with 499-nm AOD > 0.1. The retrieved fine-mode fraction of AOD is mostly between 0.7 and 1.0, and its root mean square difference (in both directions) from column-integral submicron fraction (measured with nephelometers, absorption photometers and an impactor) is 0.12. These AOD measurements from the NASA P-3 aircraft, after compensation for below-aircraft light attenuation by vertical extrapolation, mostly fall within ± 0.02 of AERONET ground-based measurements between 340-1640 nm for five overpass events. [ABSTRACT FROM AUTHOR]
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- 2011
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5. Discera quartz-crystal oscillators are ready for prime time.
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Johnson, R. Colin
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INDUSTRIAL productivity , *COMPLEMENTARY metal oxide semiconductors , *CRYSTAL oscillators , *MICROELECTROMECHANICAL systems - Abstract
The article reports on the volume production of complementary metal oxide semiconductors (CMOS) oscillators of Discera Inc. in its Canadian and Australian plant. These CMOS oscillators are quartz-crystal alternatives based on microelectromechanical systems. The company claimed that it has two mainstream customers in the last stages of qualifying its first product, the MOS-1, which has a frequency range form 1 to 125 megahertz.
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- 2007
6. Video tracking tool promises sports on cell phones.
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Johnson, R. Colin
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COMPUTER software , *ICONS (Computer graphics) , *GRAPHICAL user interfaces , *COMPUTER graphics , *STREAMING technology , *XML (Extensible Markup Language) - Abstract
Reports that a group at the University of Calgary has developed automatic recognition and tracking software using icons in Alberta. Video streaming while generating a running commentary in the form of a continuous XML document; Two-way bidirectional communication between the video server running the software and the camera; Server-client architecture.
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- 2004
7. Codec due for photorealistic JPEG2000 spec.
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Johnson, R. Colin
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COMPUTER software - Abstract
Reports on the joint venture between wavelet mathematics experts at the University of British Columbia and Image Power Inc. aimed at developing the JPEG2000 wavelet codec. Key features; Discussions on wavelet compression.
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- 1999
8. Population structure, case clusters, and genetic lesions associated with Canadian Salmonella 4,[5],12:i:- isolates.
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Clark CG, Kearney AK, Tschetter L, Robertson J, Pollari F, Parker S, Arya G, Ziebell K, Johnson R, Nash J, and Nadon C
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- Animals, Birds microbiology, Canada, DNA Transposable Elements, Drug Resistance, Bacterial, Genotype, Humans, Salmonella Infections epidemiology, Salmonella typhimurium isolation & purification, Salmonella typhimurium pathogenicity, Shellfish microbiology, Water Microbiology, Genetic Variation, Genome, Bacterial, Salmonella Infections microbiology, Salmonella typhimurium genetics
- Abstract
Monophasic Salmonella 4,[5]:12:i:- are a major public health problem because they are one of the top five Salmonella serotypes isolated from clinical cases globally and because they can carry resistance to multiple antibiotics. A total of 811 Salmonella 4,[5]:12:i:- and S. Typhimurium whole genome sequences (WGS) were generated. The various genetic lesions causing the Salmonella 4,[5]:12:i:- genotype were identified and assessed with regards to their distribution in the population of 811 Salmonella 4,[5]:12:i:- and S. Typhimurium isolates, their geographical and temporal distribution, and their association with non-human sources. Several clades were identified in the population structure, and the largest two were associated almost exclusively with a short prophage insertion and insertion of a mobile element carrying loci encoding antibiotic and mercury resistance. IS26-mediated deletions and fljB point mutants appeared to spread clonally. 'Inconsistent' Salmonella 4,[5]:12:i:- isolates associated with specific, single amino acid changes in fljA and hin were found in a single clade composed of water, shellfish, and avian isolates. Inclusion of isolates from different case clusters identified previously by PFGE validated some of the clusters and invalidated others. Some wgMLST clusters of clinical isolates composed of very closely related isolates contained an isolate(s) with a different genetic lesion, suggesting continuing mobility of the implicated element responsible. Such cases may need to be left out of epidemiological investigations until sufficient numbers of isolates are included that statistical significance of association with sources is not impaired. Non-human sources were frequently found in or near clinical case clusters. Prospective surveillance and WGS of non-human sources and retrospective analysis by WGS of isolates from existing culture collections provides data critical for epidemiological investigations of food- and waterborne outbreaks., Competing Interests: The authors have declared that no competing interests exist.
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- 2021
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9. Distribution of heavy metal resistance elements in Canadian Salmonella 4,[5],12:i:- populations and association with the monophasic genotypes and phenotype.
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Clark CG, Landgraff C, Robertson J, Pollari F, Parker S, Nadon C, Gannon VPJ, Johnson R, and Nash J
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- Animals, Antigens, Bacterial genetics, Base Sequence, Canada, Genetic Variation, Genome, Bacterial, Genotype, Humans, Interspersed Repetitive Sequences genetics, Phenotype, Phylogeny, Plasmids genetics, Salmonella typhimurium isolation & purification, Swine, Synteny genetics, Metals, Heavy toxicity, Salmonella typhimurium drug effects, Salmonella typhimurium genetics
- Abstract
Salmonella 4,[5],12:i:- are monophasic S. Typhimurium variants incapable of producing the second-phase flagellar antigen. They have emerged since the mid-1990s to become one of the most prevalent Salmonella serotypes causing human disease world-wide. Multiple genetic events associated with different genetic elements can result in the monophasic phenotype. Several jurisdictions have reported the emergence of a Salmonella 4,[5],12:i:- clone with SGI-4 and a genetic element (MREL) encoding a mercury resistance operon and antibiotic resistance loci that disrupts the second phase antigen region near the iroB locus in the Salmonella genome. We have sequenced 810 human and animal Canadian Salmonella 4,[5],12:i:- isolates and determined that isolates with SGI-4 and the mercury resistance element (MREL; also known as RR1&RR2) constitute several global clades containing various proportions of Canadian, US, and European isolates. Detailed analysis of the data provides a clearer picture of how these heavy metal elements interact with bacteria within the Salmonella population to produce the monophasic phenotype. Insertion of the MREL near iroB is associated with several deletions and rearrangements of the adjacent flaAB hin region, which may be useful for defining human case clusters that could represent outbreaks. Plasmids carrying genes encoding silver, copper, mercury, and antimicrobial resistance appear to be derived from IS26 mediated acquisition of these genes from genomes carrying SGI-4 and the MREL. Animal isolates with the mercury and As/Cu/Ag resistance elements are strongly associated with porcine sources in Canada as has been shown previously for other jurisdictions. The data acquired in these investigations, as well as from the extensive literature on the subject, may aid source attribution in outbreaks of the organism and interventions to decrease the prevalence of this clone and reduce its impact on human disease., Competing Interests: The authors have declared that no competing interests exist.
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- 2020
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10. A generalizable energetics-based model of avian migration to facilitate continental-scale waterbird conservation.
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Lonsdorf EV, Thogmartin WE, Jacobi S, Aagaard K, Coppen J, Davis A, Fox T, Heglund P, Johnson R, Jones MT, Kenow K, Lyons JE, Luke K, Still S, and Tavernia B
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- Animals, Canada, Environmental Monitoring, United States, Animal Migration, Anseriformes physiology, Energy Metabolism physiology, Models, Biological
- Abstract
Conserving migratory birds is made especially difficult because of movement among spatially disparate locations across the annual cycle. In light of challenges presented by the scale and ecology of migratory birds, successful conservation requires integrating objectives, management, and monitoring across scales, from local management units to ecoregional and flyway administrative boundaries. We present an integrated approach using a spatially explicit energetic-based mechanistic bird migration model useful to conservation decision-making across disparate scales and locations. This model moves a Mallard-like bird (Anas platyrhynchos), through spring and fall migration as a function of caloric gains and losses across a continental-scale energy landscape. We predicted with this model that fall migration, where birds moved from breeding to wintering habitat, took a mean of 27.5 d of flight with a mean seasonal survivorship of 90.5% (95% Cl = 89.2%, 91.9%), whereas spring migration took a mean of 23.5 d of flight with mean seasonal survivorship of 93.6% (95% CI = 92.5%, 94.7%). Sensitivity analyses suggested that survival during migration was sensitive to flight speed, flight cost, the amount of energy the animal could carry, and the spatial pattern of energy availability, but generally insensitive to total energy availability per se. Nevertheless, continental patterns in the bird-use days occurred principally in relation to wetland cover and agricultural habitat in the fall. Bird-use days were highest in both spring and fall in the Mississippi Alluvial Valley and along the coast and near-shore environments of South Carolina. Spatial sensitivity analyses suggested that locations nearer to migratory endpoints were less important to survivorship; for instance, removing energy from a 1036 km2 stopover site at a time from the Atlantic Flyway suggested coastal areas between New Jersey and North Carolina, including the Chesapeake Bay and the North Carolina piedmont, are essential locations for efficient migration and increasing survivorship during spring migration but not locations in Ontario and Massachusetts. This sort of spatially explicit information may allow decision-makers to prioritize their conservation actions toward locations most influential to migratory success. Thus, this mechanistic model of avian migration provides a decision-analytic medium integrating the potential consequences of local actions to flyway-scale phenomena.
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- 2016
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11. Single Center Outcomes of Status Epilepticus at a Paediatric Intensive Care Unit.
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Shah S, Shah N, Johnson R, West AN, and Prasad N
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- Adolescent, Canada, Child, Child, Preschool, Clinical Audit, Female, Humans, Infant, Male, Emergency Service, Hospital statistics & numerical data, Intensive Care Units, Pediatric statistics & numerical data, Outcome Assessment, Health Care statistics & numerical data, Status Epilepticus therapy, Transportation of Patients statistics & numerical data
- Abstract
Background: Status epilepticus (SE) is a frequent admission diagnosis to paediatric intensive care units (PICUs) and is associated with variable outcomes. We have audited our experience of patients presenting in SE at a Canadian PICU to determine unfavorable outcome variables., Methods: Charts of patients <18 years of age presenting in SE to a tertiary care PICU over a 10-year period were audited. Data were analyzed at three care-points: transport, the emergency department (ED) and the PICU. Patient outcome before PICU discharge was categorized as "favorable" for return to pre-status functioning level or "unfavorable" for new deficit/death. Student's t-test and the Kruskal-Wallis test were used for analysis of normal and skewed continuous variables, respectively, and either Chi-square test or Fisher's exact test for categorical variables., Results: 189 patients (54% males) were identified with a median age of 1.9 years. Idiopathic SE had the highest incidence; infectious/vascular etiologies were associated with more unfavorable outcomes. Progression to refractory SE in the ED had a higher incidence of death (p<0.05). Patients with an unfavorable outcome had a higher incidence of apnea during transport (p=0.01), longer hospital stays (p<0.05), need for therapeutic coma (p=0.01), longer duration of therapeutic coma (p<0.05), need for mechanical ventilation (p<0.05), and recurrent or refractory seizures during inpatient stay (p<0.05). Multivariate analysis of unfavorable outcomes of patients in SE presenting to the PICU included renal failure, cerebral edema, apnea during transport, refractory seizures, and recurrent seizures., Conclusions: Refractory seizures in children presenting with SE are associated with worsened outcomes in the PICU.
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- 2016
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12. Health professionals working with First Nations, Inuit, and Métis consensus guideline.
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Wilson D, de la Ronde S, Brascoupé S, Apale AN, Barney L, Guthrie B, Harrold E, Horn O, Johnson R, Rattray D, Robinson N, Alainga-Kango N, Becker G, Senikas V, Aningmiuq A, Bailey G, Birch D, Cook K, Danforth J, Daoust M, Kitty D, Koebel J, Kornelsen J, Tsatsa Kotwas N, Lawrence A, Mudry A, Senikas V, Turner GT, Van Wagner V, Vides E, Wasekeesikaw FH, and Wolfe S
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- Canada, Female, Humans, Health Personnel, Health Services, Indigenous, Inuit
- Abstract
Objective: Our aim is to provide health care professionals in Canada with the knowledge and tools to provide culturally safe care to First Nations, Inuit, and Métis women and through them, to their families, in order to improve the health of First Nations, Inuit, and Métis., Evidence: Published literature was retrieved through searches of PubMed, CINAHL, Sociological Abstracts, and The Cochrane Library in 2011 using appropriate controlled vocabulary (e.g.,cultural competency, health services, indigenous, transcultural nursing) and key words (e.g., indigenous health services, transcultural health care, cultural safety). Targeted searches on subtopics (e.g., ceremonial rites and sexual coming of age) were also performed. The PubMed search was restricted to the years 2005 and later because of the large number of records retrieved on this topic. Searches were updated on a regular basis and incorporated in the guideline to May 2012. Grey (unpublished) literature was identified through searching the websites of selected related agencies (e.g., Campbell Collaboration, Social Care Online, Institute for Healthcare Improvement)., Values: The quality of evidence in this document was rated using the criteria described in the Report of the Canadian Task force on Preventive Health Care (Table).
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- 2013
13. VIDAS Listeria species Xpress (LSX).
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Johnson R and Mills J
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- Canada, Environmental Microbiology, Listeria classification, Reference Standards, Reproducibility of Results, Sensitivity and Specificity, Stainless Steel, Bacteriological Techniques methods, Bacteriological Techniques standards, Enzyme-Linked Immunosorbent Assay methods, Enzyme-Linked Immunosorbent Assay standards, Listeria isolation & purification
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The AOAC GovVal study compared the VIDAS Listeria species Xpress (LSX) to the Health Products and Food Branch MFHPB-30 reference method for detection of Listeria on stainless steel. The LSX method utilizes a novel and proprietary enrichment media, Listeria Xpress broth, enabling detection of Listeria species in environmental samples with the automated VIDAS in a minimum of 26 h. The LSX method also includes the use of the chromogenic media, chromID Ottaviani Agosti Agar (OAA) and chromID Lmono for confirmation of LSX presumptive results. In previous AOAC validation studies comparing VIDAS LSX to the U.S. Food and Drug Administration's Bacteriological Analytical Manual (FDA-BAM) and the U.S. Department of Agriculture-Food Safety and Inspection Service (USDA-FSIS) reference methods, the LSX method was approved as AOAC Official Method 2010.02 for the detection of Listeria species in dairy products, vegetables, seafood, raw meats and poultry, and processed meats and poultry, and as AOAC Performance Tested Method 100501 in a variety of foods and on environmental surfaces. The GovVal comparative study included 20 replicate test portions each at two contamination levels for stainless steel where fractionally positive results (5-15 positive results/20 replicate portions tested) were obtained by at least one method at one level. Five uncontaminated controls were included. In the stainless steel artificially contaminated surface study, there were 25 confirmed positives by the VIDAS LSX assay and 22 confirmed positives by the standard culture methods. Chi-square analysis indicated no statistical differences between the VIDAS LSX method and the MFHPB-30 standard methods at the 5% level of significance. Confirmation of presumptive LSX results with the chromogenic OAA and Lmono media was shown to be equivalent to the appropriate reference method agars. The data in this study demonstrate that the VIDAS LSX method is an acceptable alternative method to the MFHPB-30 standard culture method for the detection of Listeria species on stainless steel.
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- 2013
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14. VIDAS Listeria monocytogenes II (LMO2).
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Johnson R and Mills J
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- Animals, Canada, Reproducibility of Results, Sensitivity and Specificity, Enzyme-Linked Immunosorbent Assay methods, Enzyme-Linked Immunosorbent Assay standards, Food Microbiology methods, Listeria monocytogenes isolation & purification, Meat microbiology
- Abstract
This AOAC GovVal study compared the VIDAS Listeria monocytogenes II (LMO2) to the Health Products and Food Branch MFHPB-30 reference method for detection of L. monocytogenes in ready-to-eat (RTE) meats. The VIDAS LMO2 test is an automated enzyme-linked fluorescent immunoassay for the detection of L. monocytogenes in foods. The LMO2 test, following the enrichment procedure from the MFLP-33 method, also included use of the chromogenic media, chromID Ottaviani Agosti Agar (OAA) and chromID Lmono for confirmation of LMO2 presumptive results. In previous AOAC validation studies comparing the VIDAS LMO2 method to the U.S. Food and Drug Administration Bacteriological Analytical Manual and U.S. Department of Agriculture-Food Safety and Inspection Service reference methods, LMO2 was approved as AOAC Official Method 2004.02 for the detection of L. monocytogenes in dairy products, vegetables, seafood, raw meats and poultry, and processed meats and poultry. The GovVal comparative study included 20 replicate test portions, each at two contamination levels for each matrix, where fractionally positive results (5-15 positive results/20 replicate portions tested) were obtained by at least one method at one level. Five uncontaminated controls were included. Chi-square analysis of the comparative data in this study indicates no statistical differences between the VIDAS LMO2 and the MFHPB-30 standard methods at the 5% level of significance. Confirmation of presumptive LMO2 results with the chromogenic OAA and Lmono media was shown to be equivalent to the appropriate reference method agars. The data demonstrate that the VIDAS LMO2 method is an acceptable alternative method to the MFHPB-30 standard culture method for the detection of L. monocytogenes in RTE meats, including liver paté, hot dogs, raw fermented sausage, sliced deli turkey, and sliced deli ham.
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- 2013
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15. Employment related productivity loss associated with herpes zoster and postherpetic neuralgia: a 6-month prospective study.
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Drolet M, Levin MJ, Schmader KE, Johnson R, Oxman MN, Patrick D, Fournier SO, Mansi JA, and Brisson M
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- Absenteeism, Aged, Aged, 80 and over, Canada, Female, Humans, Male, Middle Aged, Prospective Studies, Efficiency, Herpes Zoster complications, Neuralgia, Postherpetic complications
- Abstract
We conducted a prospective multi-center study to assess productivity loss associated with herpes zoster (HZ) and postherpetic neuralgia (PHN). From 10/2005 to 07/2006, we recruited immunocompetent subjects aged ≥50 years with HZ within 14 days of rash onset across Canada. Of the 249 patients recruited, 88 were employed. Data on employment status, absences from work, reasons for absence and effectiveness at work were documented at recruitment, 7-14-21-30-60-90-120-150 and 180 days later. The majority (64%) of employed subjects missed work because of HZ and 76% reported decreased effectiveness at work (i.e. presenteeism) because of HZ/PHN. Mean hours of absenteeism and presenteeism per working individual were 27 and 34 h, respectively. Pain severity and duration were associated with greater productivity loss. These results provide new information about the burden of HZ and PHN, which is useful for public health planning and cost-effectiveness analyses of HZ vaccination among individuals of working age., (Copyright © 2012 Elsevier Ltd. All rights reserved.)
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- 2012
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16. Tolerability and antibody response in adolescents and adults revaccinated with tetanus toxoid, reduced diphtheria toxoid, and acellular pertussis vaccine adsorbed (Tdap) 4-5 years after a previous dose.
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Halperin SA, McNeil S, Langley J, Blatter M, Dionne M, Embree J, Johnson R, Latiolais T, Meekison W, Noya F, Senders S, Zickler P, and Johnson DR
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- Adolescent, Adult, Aged, Canada, Diphtheria-Tetanus-acellular Pertussis Vaccines administration & dosage, Erythema chemically induced, Erythema epidemiology, Fatigue chemically induced, Fatigue epidemiology, Female, Fever chemically induced, Fever epidemiology, Headache chemically induced, Headache epidemiology, Humans, Male, Middle Aged, Pain chemically induced, Pain epidemiology, United States, Young Adult, Antibodies, Bacterial blood, Antitoxins blood, Diphtheria-Tetanus-acellular Pertussis Vaccines adverse effects, Diphtheria-Tetanus-acellular Pertussis Vaccines immunology, Immunization, Secondary adverse effects, Immunization, Secondary methods
- Abstract
Background: Although decennial adult boosters of tetanus and diphtheria toxoids are recommended in Canada and the United States, a second dose of pertussis vaccine is not currently recommended for adults., Methods: This open-label, postmarketing, multicenter study evaluated the tolerability and immunogenicity of a second dose of an adult formulation of tetanus, diphtheria, and pertussis vaccine (Tdap) in adolescents and adults 5 years after a first dose., Results: A total of 545 participants from previous Tdap vaccine studies, ranging in age from 15 to 69 years, participated in this study. Of these participants, 94.2% had at least one solicited adverse event after the booster dose such as injection-site erythema (28.6%), swelling (25.6%), or pain (87.6%) or a systemic adverse event such as myalgia (61.0%), headache (53.2%), malaise (38.2%), or fever (6.5%). These adverse events were slightly more frequent than after the initial dose. Postvaccination, 100% of participants had a tetanus antibody level ≥0.10IU/mL and 95% had a diphtheria antibody level ≥0.10IU/mL. For pertussis, 82.1% (pertussis toxoid), 96.7% (filamentous hemagglutinin), 95.6% (pertactin), and 99.8% (fimbriae) had a postvaccination antibody threshold of ≥50EU/mL., Conclusion: A second dose of Tdap vaccine 5 years after the initial dose was well tolerated and immunogenic in adolescents and adults., (Copyright © 2011 Elsevier Ltd. All rights reserved.)
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- 2011
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17. Pan-Arctic TV Series on Inuit wellness: a northern model of communication for social change?
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Johnson R, Morales R, Leavitt D, Carry C, Kinnon D, Rideout D, and Clarida K
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- Alaska, Arctic Regions, Canada, Data Collection, Greenland, Humans, Interviews as Topic, Models, Theoretical, Persuasive Communication, Health Promotion organization & administration, Inuit, Social Change, Television
- Abstract
Objectives: This paper provides highlights of a utilization-focused evaluation of a collaborative Pan-Arctic Inuit Wellness TV Series that was broadcast live in Alaska and Canada in May 2009. This International Polar Year (IPY) communication and outreach project intended to (1) share information on International Polar Year research progress, disseminate findings and explore questions with Inuit in Alaska, Canada and Greenland; (2) provide a forum for Inuit in Alaska, Canada and Greenland to showcase innovative health and wellness projects; (3) ensure Inuit youth and adult engagement throughout; and (4) document and reflect on the overall experience for the purposes of developing and "testing" a participatory communication model., Study Design: Utilization-focused formative evaluation of the project, with a focus on overall objectives, key messages and lessons learned to facilitate program improvement., Methods: Participant observation, surveys, key informant interviews, document review and website tracking., Results: Promising community programs related to 3 themes - men's wellness, maternity care and youth resilience - in diverse circumpolar regions were highlighted, as were current and stillevolving findings from ongoing Arctic research. Multiple media methods were used to effectively deliver and receive key messages determined by both community and academic experts. Local capacity and new regional networks were strengthened. Evidence-based resources for health education and community action were archived in digital formats (websites and DVDs), increasing accessibility to otherwise isolated individuals and remote communities., Conclusions: The Pan-Arctic Inuit Wellness TV Series was an innovative, multi-dimensional communication project that raised both interest and awareness about complex health conditions in the North and stimulated community dialogue and potential for increased collaborative action. Consistent with a communication for social change approach, the project created new networks, increased motivation to act and provided new tools to do so, and increased local community involvement and "voice" in the discussion and dissemination of successful strategies to promote Inuit wellness.
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- 2011
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18. Host range and lytic capability of four bacteriophages against bovine and clinical human isolates of Shiga toxin-producing Escherichia coli O157:H7.
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Niu YD, Johnson RP, Xu Y, McAllister TA, Sharma R, Louie M, and Stanford K
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- Animals, Bacteriological Techniques, Bacteriophage Typing, Bacteriophages genetics, Bacteriophages isolation & purification, Canada, Cattle, Electrophoresis, Gel, Pulsed-Field, Escherichia coli O157 isolation & purification, Genotype, Humans, Phenotype, Bacteriolysis, Bacteriophages growth & development, Escherichia coli O157 genetics, Escherichia coli O157 virology
- Abstract
Aims: To evaluate host range and lytic capability of four bacteriophages (rV5, wV7, wV8 and wV11) against Escherichia coli O157:H7 (STEC O157:H7) from cattle and humans., Methods and Results: Four hundred and twenty-two STEC O157:H7 isolates (297 bovine; 125 human) were obtained in Alberta, Canada. The four phages were serially diluted and incubated for 5 h with overnight cultures of STEC O157:H7 to estimate their multiplicity of infection (MOI). All bovine STEC O157:H7 were subjected to pulsed-field gel electrophoresis (PFGE) and phage typing (PT). Phage wV7 lysed all human and bovine isolates irrespective of PFGE genotype or PT phenotype and exhibited the lowest MOI (0.004-0.006, P < 0.0001) of all phages. Phages rV5 and wV11 exhibited a lower MOI (0.002-0.04, P < 0.0001) than did phage wV8 (25-29) and they had a narrower host range than wV7 or wV8. Phages rV5, wV11 and wV8 lysed 342 (81.0%), 321 (76.1%) and 407 (96.4%), respectively, of the 422 isolates. Susceptibility of bovine STEC O157:H7 to rV5, w11 and wV8 was influenced by PFGE genotype and/or PT phenotype., Conclusions: Phages exhibited activity against the majority of bovine and human STEC O157:H7 isolates. PFGE genotype and/or PT phenotype of the host-target influenced their vulnerability to phage attack. Susceptibility of bovine STEC O157:H7 to phage may also differ among farms. Both lytic capability and host range should be considered in the selection of therapeutic phage for on-farm control of STEC O157:H7., Significance and Impact of the Study: The present work indicates that a four-phage cocktail should be equally effective at mitigating STEC O157:H7 isolates both of bovine and of human origin. Given that some STEC O157:H7 exhibited resistance to some but not all phages, a phage cocktail is the logical approach to efficacious on-farm therapy.
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- 2009
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19. Genotypic characterization and prevalence of virulence factors among Canadian Escherichia coli O157:H7 strains.
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Ziebell K, Steele M, Zhang Y, Benson A, Taboada EN, Laing C, McEwen S, Ciebin B, Johnson R, and Gannon V
- Subjects
- Animals, Canada epidemiology, Cattle, Cattle Diseases epidemiology, Cattle Diseases microbiology, Colicins genetics, Drug Resistance, Multiple, Bacterial genetics, Escherichia coli Infections epidemiology, Escherichia coli Infections microbiology, Escherichia coli Infections veterinary, Escherichia coli O157 genetics, Genes, Bacterial, Genotype, Humans, Phenotype, Plasmids, Polymorphism, Genetic, Shiga Toxin 2 genetics, Virulence Factors genetics, Bacteriophage Typing, Escherichia coli O157 classification, Virulence Factors classification
- Abstract
In this study, the association between genotypic and selected phenotypic characteristics was examined in a collection of Canadian Escherichia coli O157:H7 strains isolated from humans and cattle in the provinces of Alberta, Ontario, Saskatchewan, and Quebec. In a subset of 69 strains selected on the basis of specific phage types (PTs), a strong correlation between the lineage-specific polymorphism assay (LSPA6) genotype and PT was observed with all strains of PTs 4, 14, 21, 31, 33, and 87 belonging to the LSPA6 lineage I (LSPA6-LI) genotype, while those of PTs 23, 45, 67, and 74 belonged to LSPA6 lineage II (LSPA6-LII) genotypes. This correlation was maintained when additional strains of each PT were tested. E. coli O157:H7 strains with the LSPA6-LI genotype were much more common in the collection than were the LSPA6-LII or lineage I/II (LSPA6-LI/II)-related genotypes (82.6, 11.2, and 5.8%, respectively). Of the strains tested, proportionately more LSPA6-LI than LSPA6-LII genotype strains were isolated from humans (52.7% versus 19.7%) than from cattle (47.8% versus 80.2%). In addition, 96.7% of the LSPA6-LII strains carried the stx(2c) variant gene, while only 50.0% of LSPA6-LI/II and 2.7% of LSPA6-LI strains carried this gene. LSPA6-LII strains were also significantly more likely to possess the colicin D gene, cda (50.8% versus 23.2%), and have combined resistance to streptomycin, sulfisoxazole, and tetracycline (72.1% versus 0.9%) than were LSPA6-LI strains. The LSPA6 genotype- and PT-related characteristics identified may be important markers of specific ecotypes of E. coli O157:H7 that have unique epidemiological and virulence characteristics.
- Published
- 2008
- Full Text
- View/download PDF
20. Information about barriers to planned change: a randomized controlled trial involving continuing medical education lectures and commitment to change.
- Author
-
Mazmanian PE, Daffron SR, Johnson RE, Davis DA, and Kantrowitz MP
- Subjects
- Behavior, Canada, Cardiovascular Diseases therapy, Humans, Primary Health Care, Surveys and Questionnaires, United States, Education, Medical, Continuing methods, Practice Patterns, Physicians'
- Abstract
Purpose: To determine whether practicing physicians receiving only clinical information at a traditional continuing medical education (CME) lecture (control group) and physicians receiving clinical information plus information about barriers to behavioral change (study group) would alter their clinical behaviors at the same rate., Method: In a randomized controlled trial, the investigators matched 13 pairs of U.S. and Canadian medical schools, assigning one school from each pair to study or control conditions. Following the commitment-to-change model, the investigators asked the primary care physicians attending control or study lectures on the management of cardiovascular risks whether they intended to make behavioral changes as a result of participating in the lectures and, if so, to indicate the specific changes. Thirty to 45 days later, the investigators surveyed the responding physicians to learn whether they had implemented those changes., Results: Information about barriers to change did not increase the likelihood that physicians in the study group would report successful changes; they were no more likely to change than those in the control group. However, the physicians in both study and control groups were significantly more likely to change (47% vs 7%, p < .001) if they indicated an intent to change immediately following the lecture., Conclusions: Successful change in practice may depend less on clinical and barriers information than on other factors that influence physicians' performances. To further develop the commitment-to-change strategy in measuring the effects of planned change, it is important to isolate and learn the powers of individual components of the strategy as well as their collective influence on physicians' clinical behaviors.
- Published
- 1998
- Full Text
- View/download PDF
21. Follow-up study of verocytotoxigenic Escherichia coli infection in dairy farm families.
- Author
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Rahn K, Renwick SA, Johnson RP, Wilson JB, Clarke RC, Alves D, McEwen SA, Lior H, and Spika J
- Subjects
- Adolescent, Adult, Agricultural Workers' Diseases epidemiology, Animals, Canada epidemiology, Carrier State, Cattle, Child, Dairying, Disease Transmission, Infectious, Escherichia coli genetics, Escherichia coli immunology, Escherichia coli Infections epidemiology, Escherichia coli O157 genetics, Escherichia coli O157 immunology, Feces microbiology, Female, Follow-Up Studies, Genes, Bacterial, Humans, Male, O Antigens immunology, Polymerase Chain Reaction, Rural Population, Agricultural Workers' Diseases microbiology, DNA, Bacterial isolation & purification, Escherichia coli isolation & purification, Escherichia coli Infections transmission, Escherichia coli Infections veterinary, Escherichia coli O157 isolation & purification
- Published
- 1998
- Full Text
- View/download PDF
22. U.S.-Canadian Consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: medical indications.
- Author
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Davis BH, Foucar K, Szczarkowski W, Ball E, Witzig T, Foon KA, Wells D, Kotylo P, Johnson R, Hanson C, and Bessman D
- Subjects
- Canada, Humans, United States, Flow Cytometry standards, Immunophenotyping standards, Leukemia pathology, Lymphoma pathology
- Published
- 1997
23. The Canadian Association of Emergency Physicians (CAEP/ACMU).
- Author
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Johnson RV
- Subjects
- Canada, Education, Medical, Continuing, History, 20th Century, Humans, Periodicals as Topic, Emergency Medicine history, Societies, Medical history
- Abstract
The Canadian Association of Emergency Physicians is a diverse group of 500 practitioners providing emergency care to the ill and injured. The specialty section of the organization represents Royal College certificants and is 120 strong. The Association, with headquarters in Ottawa, has a major interest in the continuing education of its members, policy development and the improvement of emergency health care in the country.
- Published
- 1993
- Full Text
- View/download PDF
24. Liver cirrhosis mortality and per capita alcohol consumption in Canada.
- Author
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Mao Y, Morrison H, Johnson RJ, and Semenciw R
- Subjects
- Adolescent, Adult, Aged, Alcohol Drinking epidemiology, Canada epidemiology, Humans, Middle Aged, Liver Cirrhosis mortality, Liver Cirrhosis, Alcoholic mortality
- Published
- 1992
25. [Perspectives and contributions of the social sciences to medicine in the late 20th century].
- Author
-
Johnson R
- Subjects
- Canada, Education, Medical, Social Sciences education
- Published
- 1972
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