1. Evaluation of serum bactericidal antibody assays for Haemophilus influenzae serotype a.
- Author
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Rouphael NG, Satola S, Farley MM, Rudolph K, Schmidt DS, Gomez-de-León P, Robbins JB, Schneerson R, Carlone GM, and Romero-Steiner S
- Subjects
- Adult, Canada, Colony Count, Microbial, Fluorometry methods, Humans, Microbial Viability, Middle Aged, Oxazines metabolism, Reproducibility of Results, Sensitivity and Specificity, Xanthenes metabolism, Bacteriological Techniques methods, Haemophilus Infections immunology, Haemophilus influenzae immunology, Serum Bactericidal Antibody Assay methods
- Abstract
Haemophilus influenzae type a (Hia) is an important pathogen for some American Indian, Alaskan native, and Northern Canada aboriginal populations. Assays to measure serum bactericidal activity (SBA) to Hia have not been developed or validated. Here, we describe two methods for the measurement of SBA: SBA with a viability endpoint (CFU counts) and SBA with a fluorometric endpoint using alamarBlue as the metabolic indicator. Both SBA assays measure Hia-specific functional antibody and correlate with anti-Hia IgG enzyme-linked immunosorbent assay (ELISA) concentration of naturally acquired antibodies.
- Published
- 2011
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