1. HIV-1/HIV-2 seronegativity in HIV-1 subtype O infected patients.
- Author
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Loussert-Ajaka I, Ly TD, Chaix ML, Ingrand D, Saragosti S, Couroucé AM, Brun-Vézinet F, and Simon F
- Subjects
- Acquired Immunodeficiency Syndrome diagnosis, Adult, Blotting, Western, Cameroon, Enzyme-Linked Immunosorbent Assay, France, Gene Amplification, HIV-1 genetics, HIV-1 immunology, HIV-2 genetics, HIV-2 immunology, Humans, Middle Aged, Polymerase Chain Reaction, Acquired Immunodeficiency Syndrome microbiology, HIV Seronegativity, HIV-1 classification, HIV-2 classification
- Abstract
Nine patients with atypical HIV-1 western blot profiles were diagnosed as having HIV-1 subtype O infection. All the patients were living in France; eight originated from Cameroon and one from France. Lymphocyte DNA amplification by the polymerase chain reaction was only positive when HIV-1 subtype O specific primers were used. Preliminary sequence analysis of amplified products and serological reactivity against a specific subtype O synthetic env peptide confirmed HIV-1 subtype O infection. HIV-1/HIV-2 enzyme-linked immunosorbent assays, especially those based on env peptides or on the sandwich format, can be negative in HIV-1 subtype O infection.
- Published
- 1994
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