1. A large genome center's improvements to the Illumina sequencing system.
- Author
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Quail, Michael A., Kozarewa, Iwanka, Smith, Frances, Scally, Aylwyn, Stephens, Philip J., Durbin, Richard, Swerdlow, Harold, and Turner, Daniel J.
- Subjects
NUCLEOTIDE sequence ,GENOMICS ,EQUIPMENT & supplies - Abstract
The Wellcome Trust Sanger Institute is one of the world's largest genome centers, and a substantial amount of our sequencing is performed with 'next-generation' massively parallel sequencing technologies: in June 2008 the quantity of purity-filtered sequence data generated by our Genome Analyzer (Illumina) platforms reached 1 terabase, and our average weekly Illumina production output is currently 64 gigabases. Here we describe a set of improvements we have made to the standard Illumina protocols to make the library preparation more reliable in a high-throughput environment, to reduce bias, tighten insert size distribution and reliably obtain high yields of data. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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