1. Identification of molecular markers associated with alteration of receptor-binding specificity in a novel genotype of highly pathogenic avian influenza A(H5N1) viruses detected in Cambodia in 2013.
- Author
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Rith S, Davis CT, Duong V, Sar B, Horm SV, Chin S, Ly S, Laurent D, Richner B, Oboho I, Jang Y, Davis W, Thor S, Balish A, Iuliano AD, Sorn S, Holl D, Sok T, Seng H, Tarantola A, Tsuyuoka R, Parry A, Chea N, Allal L, Kitsutani P, Warren D, Prouty M, Horwood P, Widdowson MA, Lindstrom S, Villanueva J, Donis R, Cox N, and Buchy P
- Subjects
- Adolescent, Adult, Amino Acid Substitution, Cambodia, Child, Child, Preschool, Cluster Analysis, Female, Genotype, Hemagglutinin Glycoproteins, Influenza Virus genetics, Humans, Infant, Influenza A Virus, H5N1 Subtype isolation & purification, Male, Middle Aged, Molecular Sequence Data, Mutation, Missense, Phylogeny, Sequence Analysis, DNA, Genetic Markers, Influenza A Virus, H5N1 Subtype genetics, Influenza A Virus, H5N1 Subtype physiology, Influenza, Human virology, Virus Attachment
- Abstract
Human infections with influenza A(H5N1) virus in Cambodia increased sharply during 2013. Molecular characterization of viruses detected in clinical specimens from human cases revealed the presence of mutations associated with the alteration of receptor-binding specificity (K189R, Q222L) and respiratory droplet transmission in ferrets (N220K with Q222L). Discovery of quasispecies at position 222 (Q/L), in addition to the absence of the mutations in poultry/environmental samples, suggested that the mutations occurred during human infection and did not transmit further., (Copyright © 2014, American Society for Microbiology. All Rights Reserved.)
- Published
- 2014
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