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The rapid increase in international trade volume has caused frequent fluctuation of the vessels' arrival time in container terminals. In order to solve this problem, this study proposes a methodology for rescheduling berth and quay cranes caused by updated information on the arrival time of vessels. A mixed-integer linear programming model was formulated for the berth allocation and crane assignment problem, and we solved the problem using a rolling-horizon approach. Numerical experiments were conducted under three scenarios with empirical data from a container terminal located in Busan, Korea. The experiments revealed that the proposed model reduces penalty costs and overall delayed departure time compared to the results of the terminal planner. [ABSTRACT FROM AUTHOR]

One of the 100 worst invasive exotic species, Solenopsis invicta (red imported fire ant), has the possibility to induce an allergic reaction that may eventually cause death from its aggressive stinging. In 2017, S. invicta was found at a container yard in Gamman Port, Busan, South Korea for the first time. It may result in an infestation of fire ants in the Korean environment. After this incident, sensitive quarantine procedures are required to detect possible contamination of fire ants in imported containers. However, currently, fire ant identification relies on phenotypic characteristics. This requires highly trained experts for identification and there are not enough to cover all imported containers. Here, we develop a key molecular marker to distinguish S. invicta from others using the whole genome sequence (WGS) of collected S. invicta from Gamman Port and NCBI-deposited WGS data of S.invicta and S. geminata. The consolidated genotypes of Solenopsis genus successfully indicate the distinguishable gene. The gel-based experimental validation confirmed expected classification and the developed cleaved amplified polymorphic sequences (CAPS) marker also gave a consistent result. Using the CAPS marker derived from our consolidated genotypes, the samples collected from containers in several ports can be easily tested by PCR in a few hours. The quick and easy test would increase not only the labor efficiency but also the environmental safety from fire ants. [ABSTRACT FROM AUTHOR]