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Cratons play an important role in the evolution of Earth and usually exhibit extremely thick and strong lithosphere roots and low surface heat flow. Most cratons remain in a stable state throughout their long evolutionary process. However, the stability of a few cratons was lost, the thickness of the lithosphere was greatly reduced, and surface heat flow greatly increased. Significant thinning of the lithosphere is one of the most obvious features of craton destruction. However, even within the same area, the lithospheric thickness values estimated from different methods can vary a lot. The thickness disparities between the thermal and seismic lithosphere remain unclear. On the basis of the latest global heat flow data and crustal stratification information, we calculated the thickness of the thermal lithosphere of cratons globally and conducted a detailed comparison to that of the seismic lithosphere. We found that 1) the thickness of the thermal lithosphere of most cratons is more than 150 km, while that of the Bundelkhand, Singhbhum, and upper Yangtze craton ranges from approximately 120–150 km. The thickness of the eastern North China craton, lower Yangtze craton and Wyoming craton ranges from only approximately 80–100 km. 2) For most cratons, the thickness of the seismic lithosphere is tens of kilometers (30–141 km) larger than that of the thermal lithosphere, except for the Wyoming craton, eastern North China craton, upper Yangtze craton, and Dahawar craton. 3) The part between the bottom boundaries of the seismic and thermal lithosphere can be considered as the rheological boundary layer (RBL), which is related to the craton stability. The thicker the RBL is, the higher the craton stability. 4) Due to plate subduction, the water content in the upper mantle is high, the viscosity is low, and the thickness of the RBL of cratons is small. • The thermal lithospheric thickness of most cratons exceeds 150 km. • The seismic lithosphere is thicker than thermal lithosphere for stable cratons. • Different thickness of rheological boundary layer accounts for the disparities. • Destroyed cratons usually exhibit thin rheological boundary layer. • A thick rheological boundary layer exists in stable cratons. [ABSTRACT FROM AUTHOR]