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Background: Science teaching and learning, as discursive practices, occur through the utilization of various semiotic modes. Gestures play a pivotal role as an embodied mode that acts in conjunction with speech in oral communication settings, such as classrooms. Previous research on gestures in chemistry and science education has illuminated how interactions involving different types of gestures can enhance learning opportunities for students. However, the investigation of recurrent gestures and their potential function as emblems in classroom discourse remains unexplored. Purpose: The objective of this article is to illustrate how catchments or recurrent gestures were constructed, used, and, to some extent, evolved into emblems within the interaction between an organic chemistry teacher and her students throughout an entire semester. Sample, design and methods: For the duration of an entire semester, we non-intrusively observed and recorded video footage of one class from an introductory organic chemistry course taught by an experienced university teacher. Our analysis to identify recurrent gestures involved a thorough examination of the lesson recordings. In addition, we scrutinized other semiotic modes that coincided with the recurrent gestures and assessed the metafunction of the words spoken during gesturing. Results: During our observation, we noted the occurrence of 23 catchments, with four of them recurring more than 100 times throughout the semester. These four recurrent gestures were closely tied to fundamental concepts considered crucial in the realm of organic chemistry. Remarkably, three out of these four catchments were consistently associated with visual representations. Conclusion: Recurrent gestures serve as a means to embody and impart materiality to exceedingly abstract content. By employing such gestures, the teacher managed to associate unfamiliar and abstract domains with familiar and concrete domains. This process of materialization and embodiment aids in perceiving these concepts as tangible entities that can be manipulated in accordance with their own rules.

This report provides information about a free-of-charge, trilingual (Portuguese, Spanish, and English) game-based application that engages high school and undergraduate students in reviewing the structural theory of organic compounds in a challenging way. In Time Bomb Game, students must disarm a time bomb on their own by correctly answering random questions from a database with 621 questions. Students and professors have tested and evaluated the app, and the results revealed that the game design, content, playability, and usefulness are helpful as a complementary didactic tool to provide students with the required theoretical knowledge.

The aim of this study is to better understand how teachers use various embodied semiotic modes -- speech, gestures, gaze, and proxemics -- when they present scientific content to the entire class. To analyse the multimodal practices of these teachers, we integrated the social semiotic theory of multimodality and the joint action theory in didactics. We use a case study methodology to analyse the video data and to describe the productions of two teachers from selected short episodes. The results show several similar elements of these teachers' practices. In particular, their use of several coordinated modes to make a coherent meaning to complex aspects of scientific knowledge such as three- dimensional representations of chemical structures or the relation between an experiment and its model of light diffraction. Both teachers create signs by taking everyday things from their environment to re-signify them in a scientific way. Simultaneously they maintain contact with their students through their gaze and their position. In this way, they create or strengthen the classroom habits related to the explanation of knowledge.

This study discusses the potential of the Jigsaw cooperative method in the teaching of organic chemistry. Such an approach was used in five classes with Brazilian secondary school students. Results indicate better learning regarding the development of knowledge about the nomenclature, application, and identification of functional groups at the beginning and at the end of cooperative work classes. Therefore, the Jigsaw method is an important didactic strategy to potentialize the learning of chemistry.

Recently there has been a renewed interest in the development and use of pedagogical games, as they provide an interesting approach to the appropriation of knowledge in the context of active learning. However, most didactic games fail to completely implement a cycle of reflection and action, thereby fostering mostly lower-order thinking skills and memorization as opposed to critical thinking and problem solving skills. This limitation can be traced back to the ineffective carrot and stick approach to game design that is widely applied in the context of pedagogical games. In this article, we discuss the development of a pedagogical game in which the game elements were carefully chosen to seamlessly merge with the desired chemistry content and therefore create an engaging and fun learning experience. In particular, in order to allow complex concepts to naturally emerge from a simple set of rules, we followed the so-called European approach to game design. The resulting game, MOL (Mastering the Organic chemistry Laboratory), was tested with 77 students of ages between 19 and 21 enrolled in organic chemistry courses for chemical engineering majors and 26 students of ages between 17 and 18 enrolled in a 12th grade chemistry class. The results of these classroom implementations indicate that MOL clarifies several key concepts regarding organic reactions, particularly kinetic and thermodynamic aspects. Furthermore, they suggest that MOL can be effectively used to demonstrate how the aforementioned concepts interrelate in a real laboratory situation, therefore stimulating a sense of critical thinking.

This report provides information about an interactive computer game named Say My Name that allows high school and undergraduate students to review individually nomenclature of organic compounds in an engaging and fun way by answering up to 600 questions distributed in three difficulty levels. Responses from students and teachers who have played the game have been quite positive. An assessment of students' knowledge gains was also performed; the results reveal that students who used the game as a complementary didactic tool in their studies had higher numbers of correct answers than did students who studied using conventional learning methods. Say My Name can be played in Portuguese, Spanish, and English online (free of charge) via a Web browser.

This experiment encourages students to use deductive reasoning skills to understand the correlation between different techniques used in a chemistry laboratory and to extract and analyze curcuminoids using natural products and processed food from a grocery store. Turmeric pigments were used to teach continuous or discontinuous extraction, vacuum or atmospheric pressure distillation, and thin-layer chromatography (TLC) in an introductory organic chemistry laboratory course in three laboratory sessions of 3-4 h each. Pigment extracts from the rhizome of turmeric, commercial dehydrated vegetable soup, and chicken-flavored instant bouillon (with turmeric as an ingredient) were used. The three techniques were discussed in the first laboratory session before students proposed a methodology to identify pigments in food extracts through a problem-based format. Students were assessed in a final exam where they presented a more detailed discussion of the chemistry laboratory techniques used. These results were compared to exam results from previous years. On the basis of students' and teacher's feedback, the sequence of laboratory experiments was considered interesting, and students were motivated to participate more actively during instruction.

This report provides information about an interactive computer game that allows undergraduate students to review individually stereochemistry topics in an engaging way by responding to 230 novel questions distributed at three difficulty levels. Responses from students and instructors who have played the game have been quite positive. Stereogame is available free of charge in Portuguese and English and can be played online via a Web browser or in printable form (board game, cards, and answer key) that is also available for download.

The substitution of petroleum-based fuels with those from renewable sources has gained momentum worldwide. A UV-vis experiment for the quantitative analysis of biofuels (bioethanol or biodiesel) in (petroleum-based) diesel oil has been developed. Before the experiment, students were given a quiz on biofuels, and then they were asked to suggest a suitable UV-vis experiment for the quantification of biofuels in diesel oil. After discussing the results of the quiz, the experiment was conducted. This included the determination of [lambda][subscript max] of the medium-dependent, that is, solvatochromic, visible absorption band of the probe 2,6-bis[4-("tert"-butyl)phenyl]-4-{2,4,6-tris[4-("tert"-butyl)phenyl]pyridinium-1-yl}phenolate as a function of fuel composition. The students appreciated that the subject was linked to a daily situation and that they were asked to suggest the experiment. This experiment served to introduce the phenomena of solvation and solvatochromism. (Contains 6 figures.)

Keywords: ICS-30 EN ICS-30 939 943 5 09/25/23 20230901 NES 230901 The biennial International Carbohydrate Symposium (ICS) has been organized by the national representatives of the International Carbohydrate Organization (ICO) since 1960, bringing together thousands of worldwide attendees aiming to learn and discuss all aspects related to carbohydrate research and its growing perspectives for future projects. 5: Professor Yasuhiro Kajihara (Osaka University, Japan) receiving the Roy L Whistler International Award in Carbohydrate chemistry from ICO President, Professor Amélia P. Rauter. [Extracted from the article]

It is my sad duty to put together a few words in appreciation of my close friend and esteemed scientific colleague Faruk Nome who died on September 24, 2018. He stands high in the list of the most productive and talented physical organic chemists of the last hundred years. [ABSTRACT FROM AUTHOR]

[Display omitted] • Prescribed fire (PF) is a land management practice. • Knowledge on PF application in Brazilian mountain environments is scarce. • Soil properties and organic matter chemistry were monitored in the short and medium term after PF application. • Organic matter recovered to its initial condition after 240 days since PF. Prescribed fire (PF) is a management practice used ecosystems where the occurrence of wildfires is high due to the accumulation of biomass on the surface. In general, studies dealing with fire-affected soils evidence the degradation of less thermo-labile structures, with the preservation of more thermo-stable phases. As studies in mountain environments in Brazil are scarce and considering that the practice of PF has been on the rise in recent years, the present study aimed to quantifying and characterizing the impact of PF on soil organic matter in the Itatiaia National Park, Rio de Janeiro-Brazil. In a 240 days period, we assessed the impact of PF on soil pH, base cations release, available P, C and N contents and stocks, soil organic matter (SOM) quality. Among other techniques, we applied ATR-FTIR and 13 C NMR spectroscopy. Soil chemical and physical attributes were also quantified. At 10 days, PF application caused the release of labile organic carbon (LOC) forms, inducing an increase in content of humic fractions in the form of C-FA and C-HA. The humification process, nevertheless, occurred at a lower speed than the incorporation of LOC. Humin contents, in fact, did not systematically increase with time. At 10 days following PF, a decrease in carbohydrates and peptides occurred, with greater HA aromaticity. After 240 days since PF, the structural characteristics of HA were restored to levels like the original (pre-PF) condition. At 120 days after PF, the pH value, base saturation, Mg, Na and P increased in soil. However, the amount of C and N accumulated in the soil did not increase with PF application. We observed that up to 120 days of PF, the mineralization process seems to occur in priority over humification. A period longer than 240 days seems anyway needed to restore soil C and N stocks. [ABSTRACT FROM AUTHOR]

Propolis is a complex mixture of resinous and balsamic material collected from the exudates of plants, shoots, and leaves by bees. This study evaluated red propolis extracts obtained by conventional (ethanolic) extraction and ultrasound-assisted extraction of six samples from different regions of northeastern Brazil. The total phenolic compounds and flavonoids, in vitro antioxidant activity, concentration of formononetin and kaempferol and the cytotoxicity against four human tumor cell lines were determined for all twelve obtained extracts. Significant variations in the levels of the investigated compounds were identified in the red propolis extracts, confirming that the chemical composition varied according to the sampling region. The extraction method used also influenced the resulting propolis compounds. The highest concentration of the compounds of interest and the highest in vitro antioxidant activity were exhibited by the extracts obtained from samples from state of Alagoas. Formononetin and kaempferol were identified in all samples. The highest formononetin concentrations were identified in extracts obtained by ultrasound, thus indicating a greater selectivity for the extraction of this compound by this method. Regarding cytotoxic activity, for the HCT-116 line, all of the extracts showed an inhibition of greater than 90%, whereas for the HL-60 and PC3 lines, the minimum identified was 80%. In general, there was no significant difference (p>0.05) in the antiproliferative potential when comparing the extraction methods. The results showed that the composition of Brazilian red propolis varies significantly depending on the geographical origin and that the method used influences the resulting compounds that are present in propolis. However, regardless of the geographical origin and the extraction method used, all the red propolis samples studied presented great biological potential and high antioxidant activity. Furthermore, the ultrasound-assisted method can be efficiently applied to obtain extracts of red propolis more quickly and with high concentration of biomarkers of interest. [ABSTRACT FROM AUTHOR]

Nutrition during pregnancy is one of the key elements to good maternal and child health, as well as to lifetime landmarks. However, many pregnant women go undernourished in less developed settings. The purpose of this study was to estimate the prevalence and factors associated with gestational night blindness (GXN) and maternal anemia in a cross-sectional population-based study in Cruzeiro do Sul, Acre State, Western Brazilian Amazon. All women living in the municipality admitted at the only maternity-hospital in the city to delivery of a singleton infant were eligible to this study (n = 1,525). Recruitment of participants took place between July 2015 to June 2016. GXN was assessed in the postpartum period by WHO standardized interview. Maternal anemia was defined as hemoglobin at delivery < 110.0 g/L. We estimated prevalence rates and adjusted prevalence ratios (aPR), alongside 95% confidence intervals (95% CI), of the factors associated with the outcomes through multiple Poisson regression models with robust variance. Alarming prevalence of GXN (11.5%; 95% CI, 9.97–13.25) and maternal anemia (39.4%; 95% CI, 36.84–41.95) were found. Factors associated with GXN were (aPR; 95% CI): ≥ 5 residents in the household (2.06; 1.24–3.41), smoking during pregnancy (1.78; 1.15–2.78), and attending < 6 antenatal care visits (1.61; 1.08–2.40). Factors associated with maternal anemia were (aPR; 95% CI): maternal age < 19 years (1.18; 1.01–1.38), gestational malaria (1.22; 1.01–1.49), not taking micronutrient supplements during pregnancy (1.27; 1.01–1.62), and attending < 6 antenatal care visits (1.40; 1.15–1.70). High prevalence rates of GXN and maternal anemia in these postpartum women may reflect poor assistance during antenatal care, underlying the importance of rethinking current protocols related to nutrition in pregnancy. [ABSTRACT FROM AUTHOR]

The selection of viral strains with resistance-associated substitutions at hepatitis C virus (HCV) NS5A and NS5B genes is considered one of the limiting factors for achieving sustained virologic response (SVR) to combination of direct-acting antivirals daclatasvir (DCV) and sofosbuvir (SOF). Since 2015, this interferon-free regimen has been available in Brazilian clinical routine for treating mono- and HCV/HIV-coinfected patients chronically infected with genotypes 1 and 3. Our aim was to assess SVR rate for Brazilian patients chronically infected with genotypes 1 and 3 after DCV/SOF therapy and the frequency of baseline RASs in HCV NS5A and NS5B genes. Serum samples were collected from 107 monoinfected patients and 25 HCV/HIV co-infected patients before antiviral therapy with DCV/SOF. Genetic diversity of NS5A and NS5B genes was assessed by direct nucleotide sequencing. Overall, SVR rate was 95.4% (126/132), and treatment failure occurred in five monoinfected and one HCV/HIV co-infected patient. NS5A RASs frequency was higher for HCV/HIV patients (28%) than monoinfected patients (16.8%). No difference was evidenced between mono- and HCV/HIV-coinfected groups (15% vs. 16%) regarding NS5B gene. Genotype (GT) 1b strains had significantly more baseline substitutions in NS5A (31.6%) than GT 1a and 3a. At least one primary NS5A RAS described in literature at loci 28, 30, 31 or 93 was identified in HCV GTs 1 strains for both groups. As for NS5B, RASs at positions 159 and 316 was observed only in GT 1b strains. This study highlighted that SVR rate in clinical routine in Brazil was similar to randomized clinical trials (89–98%). Our research provided genetic data about the circulation of resistant variants in Brazil. Despite its presence, most of identified baseline mutations did not negatively impact treatment outcome. Genetic diversity of circulating strains suggested that most of the Brazilian HCV chronic carriers are susceptible to new therapeutic regimens including recently approved DAAs. [ABSTRACT FROM AUTHOR]

Background: Standard human landing catches (sHLCs) have historically been a key component of Onchocerca volvulus transmission monitoring, but expose health-workers to potentially hazardous vector bites. Novel human-bait-free trapping methods have been developed, but do not always work where they are needed and may not generate O. volvulus surveillance data that is directly comparable with historic data. Methodology: Simuliid sHLCs and mineral-oil protected HLCs (mopHLCs) were performed in a rural village of Amazonas state, Brazil. A four-hour direct comparisons of sHLCs and mopHLCs was carried-out using six vector collectors, each of whom used one leg for a HLC and one for a mopHLC. Two-person collection teams then exclusively performed either mopHLCs or sHLCs for a further set of 12 four-hour collections. Following the completion of all collections, simuliid-bite mark estimates were made from legs used exclusively in sHLCs and legs used exclusively in mopHLCs. Principal findings: All of the 1669 captured simuliids were identified as the O. volvulus vector Simulium oyapockense. Overall, mopHLC simuliids captured per hour (S/H) rates were lower than those obtained with sHLC trapping (15.5 S/H versus 20 S/H). Direct comparisons of simuliid capture rates found that vector-collectors captured simuliids significantly more efficiently (: 20.5 S/H) with mopHLC trapping than with sHLC trapping (: 16.4 S/H): P-value = 0.002. MopHLCs performed in isolation were, however, observed to capture vectors less efficiently (: 13.4 S/H) than sHLCs performed under similar conditions (: 19.98 S/H). All six vector collectors had significantly higher simuliid capture per counted bite mark (SC/CBM) rates using mopHLCs than they were observe to have using sHLCs (: 21 SC/CBM versus : 1 SC/CBM; p-value = 0.03125). Conclusions: Vector collectors captured significantly more simuliids per counted bite mark with mopHLCs than with sHLCs. Further investigations into the utility of mopHLCs for onchocerciasis xenomonitoring and beyond are merited. [ABSTRACT FROM AUTHOR]

Introduction: Studies on metabolomics and CKD have primarily addressed CKD incidence defined as a decline on eGFR or appearance of albuminuria in the general population, with very few evaluating hard outcomes. In the present study, we investigated the association between metabolites and mortality and ESRD in a CKD cohort. Setting and methods: Data on 454 participants of the Progredir Cohort Study, Sao Paulo, Brazil were used. Metabolomics was performed by GC-MS (Agilent MassHunter) and metabolites were identified using Agilent Fiehn GC/MS and NIST libraries. After excluding metabolites present in <50% of participants, 293 metabolites were analyzed. An FDR q value <0.05 criteria was applied in Cox models on the composite outcome (mortality or incident renal replacement therapy) adjusted for batch effect, resulting in 34 metabolites associated with the outcome. Multivariable-adjusted Cox models were then built for the composite outcome, death, and ESRD incident events. Competing risk analysis was also performed for ESRD. Results: Mean age was 68±12y, mean eGFR-CKDEPI was 38.4±14.6 ml/min/1.73m2 and 57% were diabetic. After adjustments (GC-MS batch, sex, age, DM and eGFR), 18 metabolites remained significantly associated with the composite outcome. Nine metabolites were independently associated with death: D-malic acid (HR 1.84, 95%CI 1.32–2.56, p = 0.0003), acetohydroxamic acid (HR 1.90, 95%CI 1.30–2.78, p = 0.0008), butanoic acid (HR 1.59, 95%CI 1.17–2.15, p = 0.003), and docosahexaenoic acid (HR 0.58, 95%CI 0.39–0.88, p = 0.009), among the top associations. Lactose (SHR 1.49, 95%CI 1.04–2.12, p = 0.03), 2-O-glycerol-α-D-galactopyranoside (SHR 1.76, 95%CI 1.06–2.92, p = 0.03), and tyrosine (SHR 0.52, 95%CI 0.31–0.88, p = 0.02) were associated to ESRD risk, while D-threitol, mannitol and myo-inositol presented strong borderline associations. Conclusion: Our results identify specific metabolites related to hard outcomes in a CKD population. These findings point to the need of further exploration of these metabolites as biomarkers in CKD and the understanding of the underlying biological mechanisms related to the observed associations. [ABSTRACT FROM AUTHOR]

Background: Anemia is recognized as a major public health problem in childhood, especially in children under 24 months of age. Despite improvements in public health strategies to prevent and control anemia in Brazilian young children in the last decade, few studies have assessed the predictors for this condition in primary health care. Thus, this study aimed to assess the associated factors of anemia in young children who visited primary public health care facilities in Brazil. Methods: A cross-sectional study was conducted with 520 children aged 11 to 15 months who visited the primary health care in four Brazilian cities. Anemia was defined as hemoglobin concentration < 110 g/L in venous blood samples. Multilevel Poisson regression models were used to describe the associations between anemia and independent variables. Results: The frequency of anemia was 23.1%. A higher frequency was observed in children who live with more than one other child younger than 5 years in the house (Prevalence Ratio [PR] 1.47; 95% Confidence Interval [CI] 1.01–2.14), who started to receive fruits and vegetables after 8 months of age (PR 1.92; 95% CI 1.19–3.10), who were stunted (PR 2.44; 95% CI 1.32–4.50), who were hospitalized at least once in their life (PR 1.55; 95% CI 1.03–2.33) and who were in the lower tertile of serum folate concentration (PR 2.24; 95% CI 1.30–3.85). Conclusions: Inadequate complementary feeding practices and morbidity were the main predictors for anemia in early childhood in this population. Improvements in current strategies to promote healthy complementary feeding along with better control of morbidities are recommended to reduce anemia in Brazilian young children. [ABSTRACT FROM AUTHOR]

Propolis is a complex bioactive mixture produced by bees, known to have different biological activities, especially in countries where there is a rich biodiversity of plant species. The objective of this study was to determine the chemical composition and evaluate the antioxidant and cytotoxic properties of Brazilian propolis from the species Plebeia droryana and Apis mellifera found in Mato Grosso do Sul, Brazil. In the ethanolic extracts of P. droryana propolis (ExEP-P) and A. mellifera (ExEP-A) acids, phenolic compounds, terpenes and tocopherol were identified as major compounds. Both extracts presented antioxidant activity against the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical, the maximum activities being 500 μg/mL (ExEP-P) and 300 μg/mL (ExEP-A). However, only ExEP-A was able to inhibit lipid peroxidation induced by the oxidizing agent 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH), which inhibited oxidative hemolysis and reduced the levels of malondialdehyde (MDA) in human erythrocytes for 4 h of incubation. The extracts also reduced the cell viability of the K562 erythroleukemia tumour line, with a predominance of necrotic death. Thus, it is concluded that the propolis produced by P. droryana and A. mellifera contain important compounds capable of minimizing the action of oxidizing substances in the organism and reducing the viability of erythroleukemia cells. [ABSTRACT FROM AUTHOR]

The mosquito Aedes aegypti is the primary vector of different arboviruses and represents a major public health problem. Several Brazilian populations of Ae. aegypti have developed resistance to temephos, the most used organophosphate larvicide. New tools which are less harmful to the environment and safer for humans are becoming increasingly important to control this insect vector. Spinosad, an aerobic fermentation product of a soil actinobacteria, has a favorable environmental profile. It presents selective insecticide properties, a mechanism of action that differs from those of many synthetic chemical insecticides. The toxicity of spinosad and temephos to Aedes aegypti populations from Brazil, which were previously exposed to temephos, were investigated in this study. Larval susceptibility (LC50) to temephos varied from 3μg/L for Rockefeller up to 260 μg/L for Santana do Ipanema field derived population. Larval susceptibility (LC50) to spinosad varied from 23μg/L for Rockefeller up to 93μg/L for Marilia field derived population. In addition, a semi-field trial was performed to evaluate spinosad (NatularTM DT) initial efficacy and persistence toward four field-derived lineages and the Rockefeller lineage, used as an internal control. Spinosad was tested at 0.5mg active ingredient/L in 200L capacity water tanks. Mortality was recorded each 24 hours after exposition and tanks were further recolonized once per week with mortality being recorded daily for eight weeks. Spinosad provided a level equal or superior to 80% mortality during a seven to eight week evaluation period. The assessed populations did not present cross-resistance between spinosad and temephos in laboratory conditions. It demonstrates that spinosad may be a promising larvicide for the control of Ae. aegypti, especially for populations in which resistance to temephos has been detected. [ABSTRACT FROM AUTHOR]

Candida albicans caused 44% of the overall candidemia episodes from 2006 to 2010 in our university tertiary care hospital. As different antifungal agents are used in therapy and also immunocompromised patients receive fluconazole prophylaxis in our institution, this study aimed to perform an antifungal susceptibility surveillance with the C.albicans bloodstream isolates and to characterize the fluconazole resistance in 2 non-blood C.albicans isolates by sequencing ERG11 gene. The study included 147 C. albicans bloodstream samples and 2 fluconazole resistant isolates: one from oral cavity (LIF 12560 fluconazole MIC: 8μg/mL) and one from esophageal cavity (LIF-E10 fluconazole MIC: 64μg/mL) of two different patients previously treated with oral fluconazole. The in vitro antifungal susceptibility to amphotericin B (AMB), 5-flucytosine (5FC), fluconazole (FLC), itraconazole (ITC), voriconazole (VRC), caspofungin (CASP) was performed by broth microdilution methodology recommended by the Clinical and Laboratory Standards Institute documents (M27-A3 and M27-S4, CLSI). All blood isolates were classified as susceptible according to CLSI guidelines for all evaluated antifungal agents (MIC range: 0,125–1.00 μg/mL for AMB, ≤0.125–1.00 μg/mL for 5FC, ≤0.125–0.5 μg/mL for FLC, ≤0.015–0.125 μg/mL for ITC, ≤0.015–0.06 μg/mL for VRC and ≤0.015–0.125 μg/mL for CASP). In this study, we also amplified and sequenced the ERG11 gene of LIF 12560 and LIF-E10 C.albicans isolates. Six mutations encoding distinct amino acid substitutions were found (E116D, T128K, E266D, A298V, G448V and G464S) and these mutations were previously described as associated with fluconazole resistance. Despite the large consumption of antifungals in our institution, resistant blood isolates were not found over the trial period. Further studies should be conducted, but it may be that the very prolonged direct contact with the oral antifungal agent administered to the patient from which was isolated LIF E-10, may have contributed to the development of resistance. [ABSTRACT FROM AUTHOR]

Background: Multiple micronutrients in powder (MNP) are recommended by WHO to prevent anemia in young children. However, evidences for its effectiveness in different populations and improvements in other outcomes (e.g. linear growth and vitamin A deficiency) are scarce. Methods: A multicentre pragmatic controlled trial was carried out in primary health centres. At study baseline, a control group (CG) of children aged 10- to 14 months (n = 521) was recruited in the routine healthcare for assessing anemia, anthropometric and micronutrient status. At the same time, an intervention group (IG) of infants aged 6- to 8 months (n = 462) was recruited to receive MNP daily in complementary feeding over a period of 60 days. Both study groups were compared when the IG infants reached the age of the CG children at enrolment. Results: In CG, the prevalence of anemia [hemoglobin (Hb) < 110 g/L], iron deficiency (ID, plasma ferritin < 12 μg/L or TfR > 8.3 mg/L), and vitamin A deficiency (VAD, serum retinol < 0.70μmol/L) were 23.1%, 37.4%, and 17.4%, respectively. Four to six months after enrolment, when the IG participants had the same age of the controls at the time of testing, the prevalence of anemia, ID and VAD in IG were 14.3%, 30.1% and 7.9%, respectively. Adjusting for city, health centre, maternal education, and age, IG children had a lower likelihood of anemia and VAD [Prevalence Ratio (95% CI) = 0.63 (0.45, 0.88) and 0.45 (0.29, 0.69), respectively] when compared with CG children. The adjusted mean distributions of Hb and length-for-age Z-scores improved by 2 SE in the IG compared to CG children. Conclusions: MNP effectively reduced anemia and improved growth and micronutrient status among young Brazilian children. Trial Registration: Registro Brasileiro de Ensaios Clinicos [ABSTRACT FROM AUTHOR]

The present study aimed to evaluate the effect of seasonal and diurnal events on the chemical profile of the essential oil obtained from the leaves of Copaifera langsdorffii Desf. This study was performed in a Brazilian savanna named Cerrado. We identified the best harvesting period for obtaining the highest amount of compounds used for commercial and industrial purposes. The chemical profile of the essential oils was evaluated by GC-FID and GC-MS, and the results were assessed through multivariate analyses. The data showed that the time of day and seasonal variations affect the quality of the essential oil obtained. Leaves harvested at the end of the day (5:00 pm) in the dry season resulted in richer essential oils with higher amounts of non-oxygenated sesquiterpenes. To the best of our knowledge, environmental conditions induce metabolic responses in the leaves of C. langsdorffii, which changes the patterns of sesquiterpene production. Therefore, these factors need to be considered to obtain better concentrations of bioactive compounds for pharmacological studies. [ABSTRACT FROM AUTHOR]

From the very beginning of civilization, humans have used chemicals from Nature - most of them comparatively small organic molecules now designated as secondary metabolites - for a variety of purposes such as pigments and dyes, arrow and fish poisons and olfactory stimulants. It is no wonder that many eminent chemists were attracted to these materials to elucidate the underlying chemistry. Willstatter, Baeyer, Richard Kuhn, Karrer, Robinson and others made pioneering studies and isolated several new compounds which were included in the curriculum of earlier days. Another pioneer, A G Perkin, second son of Sir W H Perkin, was, according to his elder brother, W H Perkin, Jr, a dabhand with natural dyes.He andAE Everest wrote a book The Natural Organic Colouring Matters, first published in 1918, which was like a Bible to natural product chemists of yesteryears. (You can now read it on line!). As organic chemistry progressed, making leaps and bounds, the newer exciting discoveries gradually pushed the study of several of these compounds out of the curriculum. While one can understand this trend, one also feels sad that students these days are not aware of several interesting facets of natural products chemistry which link organic chemistry with folklore, traditional practices by diverse native communities, indigenous systems of medicine and current ideas of chemical structure and reactivities. It is not possible to fill in these gaps in textbooks which are designed to cater to the needs of students preparing for various university examinations, but articles such as those contemplated here can provide students with knowledge that can be stimulating, interesting as well as enjoyable! We begin the series with 'some exotic red pigments of plant origin'. Several red coloured dyes and pigments of vegetable origin have been known to mankind ever since the dawn of civilization. Their varied uses have been mentioned in ancient literature, including the Ramayana, folklores, travelogues and accounts of several explorers. Their chemistry is equally fascinating and has attracted the attention of a number of eminent organic chemists. In this article, we examine a few of these pigments isolated from plant materials found in different parts of the world. They include the pigments of the red sandalwood, the colourants of safflower, the exotic cosmetic chica red, brazilin from the Brazil wood (and the related hematoxylin), the compounds from the Dragon's blood, the sesquiterpene quinones of the Miro wood and rottlerin, the main red pigment of Kamala dye. [ABSTRACT FROM AUTHOR]