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• Change in land use has changed the carbon and nitrogen stocks in the soil. • The permanence time of the cover crops affected the carbon dynamics in the soil. • The stable fraction of organic matter contributed to soil carbon accumulation. Soil organic carbon (SOC) is the main terrestrial carbon reservoir. The spatial distribution of SOC is mainly regulated by environmental factors and anthropogenic activity. Historically, land-use change has been responsible for much of the world's greenhouse gas emissions. However, carbon storage can be improved through agricultural management, either by increasing carbon inputs through higher crop yields or by delaying the release of carbon into the atmosphere. Therefore, it is essential to correctly choose which cover crops to use and how long these crops will remain in the crop rotation system. In order to clarify these issues, the objective of this work was to evaluate the chemical quality and changes in soil carbon stock between no-tillage and soybean/corn crop succession systems and integrated crop-livestock systems. The study was carried out in Querência (MT), Brazil, located in the Cerrado/Amazon ecotone. The integrated systems were studied between 2007 and 2014. These systems followed annual crop rotation and forage rotation, and were laid out as a consortium or were isolated, with the aim of producing grain and meat. In order to determine soil chemical properties, 170 sampling plots were randomized among the treatments. The highest carbon values were observed in the integrated crop-livestock systems (ICL), probably due to higher inputs of organic residues. Crop rotation with plants of different families allowed better land use and soil exploration. However, integrated production systems comprising longer pasture periods, no-tillage, and soybean/corn crop succession decreased soil carbon. When comparing carbon stocks from 2010 to 2014, only integrated production systems ICL2 and ICL3 were able to increase carbon stocks. Our results highlight the importance of length of growing and correct choice of cover crops in maintaining SOC stocks, and thus provide insights to improve SOC stocks in agricultural land in Brazil's main producing region. [ABSTRACT FROM AUTHOR]