1. Saliva and blood interferon gamma levels and IFNG genotypes in acute graft-versus-host disease.
- Author
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Resende, RG, Correia‐Silva, JDF, Silva, TA, Xavier, SG, Bittencourt, H, Gomez, RS, and Abreu, MHNG
- Subjects
STEM cell transplantation ,ACADEMIC medical centers ,CHI-squared test ,ENZYME-linked immunosorbent assay ,FISHER exact test ,GENES ,GENETIC polymorphisms ,GRAFT versus host disease ,INTERFERONS ,LONGITUDINAL method ,SALIVA ,STATISTICS ,U-statistics ,DATA analysis ,DATA analysis software ,DESCRIPTIVE statistics - Abstract
Oral Diseases (2012) 18, 816-822 Objective: Graft-versus-host disease is a major complication after allogenic hematopoietic stem cell transplantation. Interferon gamma is an important pro-inflammatory cytokine involved in this disease. Cytokine gene polymorphisms are associated with functional differences in cytokine expression and can alter the clinical course of graft-versus-host disease. This study aimed to investigate the association between IFN-γ levels in saliva, blood, and IFNG polymorphisms, as well as the occurrence of acute graft-versus-host disease in allogenic HSCT. Subjects and Methods: Fifty-eight consecutive allogenic hematopoietic stem cell transplantation recipients and their donors were prospectively studied. IFN-g levels in saliva and blood were assessed by ELISA. Samples were collected weekly from 7 days before transplantation (day −7) to 100 days after allogenic HSCT (day +100) or until death. Saliva and/or blood samples were obtained from the recipients and donors to determine IFNG gene polymorphisms. Results: Increased saliva and blood IFN-g levels were observed in patients that had developed aGVHD. In the saliva, the peak levels of IFN-g could be found one week before aGVHD diagnosis, while in the blood, peak levels of IFN-g could be only observed upon diagnosis. A significant association could be identified between the recipients' IFNG genotypes and the IFN-g levels in their blood, at +14 days after HSCT. No association could be observed between IFNG gene polymorphisms and the aGVHD. Conclusion: The present study shows that the genetic background of recipients can influence the production of IFN-g. Moreover, as IFN-g levels in the saliva and blood were found to be associated with aGVHD development, this cytokine may be a useful predictor of acute graft-versus-host disease. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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