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Antimicrobial resistance (AMR) is an increasing and urgent issue for human health worldwide, as it leads to the reduction of available antibiotics to treat bacterial infections, in turn increasing hospital stays and lethality. Therefore, the study and genomic surveillance of bacterial carriers of resistance in and outside of clinical settings is of utter importance. A colony of multidrug resistant (MDR) bacteria identified as Klebsiella spp., by 16S rDNA amplicon sequencing, has been isolated from an urban lake in Brazil, during a drug-degrading bacterial prospection. Genomic analyses revealed the bacteria as Klebsiella pneumoniae species. Furthermore, the in silico Multilocus Sequence Typing (MLST) identified the genome as a new sequence type, ST5236. The search for antimicrobial resistance genes (ARGs) detected the presence of genes against beta-lactams, fosfomycin, acriflavine and efflux pumps, as well as genes for heavy metal resistance. Of particular note, an extended-spectrum beta-lactamase gene (blaCTX-M-15) has been detected in close proximity to siphoviridae genes, while a carbapenemase gene (KPC-2) has been found in an extrachromosomal contig, within a novel non-Tn4401 genetic element (NTEKPC). An extrachromosomal contig found in the V3 isolate is identical to a contig of a K. pneumoniae isolate from a nearby hospital, which indicates a putative gene flow from the hospital network into Paranoá lake. The discovery of a MDR isolate in this lake is worrisome, as the region has recently undergone periods of water scarcity causing the lake, which receives treated wastewater effluent, and is already used for recreational purposes, to be used as an environmental buffer for drinking water reuse. Altogether, our results indicate an underrepresentation of environmental K. pneumoniae among available genomes, which may hamper the understanding of the population dynamics of the species in the environment and its consequences in the spread of ARGs and virulence genes. [ABSTRACT FROM AUTHOR]

Antimicrobial-resistant bacteria (ARB) carrying antimicrobial resistance genes (ARGs) have been increasingly detected in water sources. Pseudomonas sp. are opportunistic pathogens commonly reported in water samples and different antimicrobial resistance mechanisms have been described in Pseudomonas sp., producing multidrug-resistant (MDR) phenotype. Besides, the presence of heavy metal resistance genes (HMRGs) may select ARB, which is worrying. Therefore, this study aimed to characterize the genotypes of Pseudomonas sp. isolated from different water sources. Water samples (i.e., rivers, streams, lakes and sewage treatment plants) were collected from different cities in Brazil. The bacterial identification was performed by sequencing the 16S rDNA and the resistance profile to antimicrobials and heavy metals were determined by minimum inhibitory concentration (MIC). Several ARGs, HMRGs, and plasmids were researched by PCR and the amplicons were sequenced for confirmation. A total of 23 Pseudomonas sp. isolates were obtained and identified as Pseudomonas saponiphila, Pseudomonas hunanensis, Pseudomonas aeruginosa, and Pseudomonas asiatica. These isolates presented high MICs to antimicrobials and heavy metals, being the great majority (n = 21; 91%) classified as MDR. Different clinically important ARGs were detected, such as blaGES, qnrS, qepA, tetB, aac(3′)-IIa, and ant(2″)-Ia. The czcA gene was the only HMRG detected and no plasmids were found. To the best of our knowledge, this is the first report of the world of P. saponiphila carrying ARGs (i.e., blaGES, qnrS, aac(3′)-IIa, tetB) and QepA-producing P. hunanensis and the first time of P. saponiphila, P. asiatica, and P. hunanensis in Brazil. [ABSTRACT FROM AUTHOR]

ABSTRACT Manzolli, R.P.; Portz, L.C.; Bitencourt, V.J.B.; Leal, R.A.; Martins, E.M.; Biancini da Silva, A.; Barboza, E.G.; Caron, F.; Alcantará-Carrió, J., and Sawakuchi, A.O., 2018. Process control in the geneses and evolution of a lagoonbarrier system inside of the Patos lagoon, South of Brazil. In: Shim, J.-S.; Chun, I., and Lim, H.S. (eds.), Proceedings from the International Coastal Symposium (ICS) 2018 (Busan, Republic of Korea). Journal of Coastal Research, Special Issue No. 85, pp. 651–655. Coconut Creek (Florida), ISSN 0749-0208. The origin and geological evolution of a complex of a beach ridgeplain in the Feitoria lagoon-barrier, located on the western margin of the southern cell Patos lagoon, Brazil was influenced by the interactions between the alocyclic (climate change and relative sea level) and autocyclic (sediment supply, waves, longshore drift and storm surges) forcing. The study of this regressive beach ridgeplain included the analysis of orthophotos; topographic detail (PRO-XRS Trimble® – post-processed); and shallow geophysical data with Ground Penetrating Radar (GPR), 150, 200 and 400 MHz antennae, combined with facies analysis and radiocarbon dating (AMS) and Optically Stimulated Luminescence (OSL) from shallow borehole samples. The analysis of orthophotos allowed for the definition of at least nine morphologically distinct series of progradation, marked by truncations of progressive orientation changes. The integration of topographically corrected GPR data, sedimentary records, and geochronological data determined the beginning of the progradation occurred at 7.2 kaBP. At the beginning of progradation, the low tide terrace was at the height of 1.9m (EGM96) Above the Sea Current Level (ASCL). The swash zone was in the 2.5m, and the crest reached 4.3m ASCL. Currently, the low tide terrace quota is −0.4m, the swash zone to 0.3m and the crest reaches 2.1m ASCL. Among other factors, the fall of the lagoon base levels was associated with sea level fall during the Holocene regression. However, crest construction control is dependent on the lagoon base level oscillation, which in turn is controlled by the precipitation regime and storms surges. Moreover, these results suggest that the orientation of the ridges was controlled by changes in the internal lagoon hydrodynamics, due to the progressive narrowing of the lagoon connection with the open ocean. [ABSTRACT FROM AUTHOR]

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Copyright of Ciencia, Cuidado e Saude is the property of Universidade Estadual de Maringa and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Abstract: This study characterized the variability in magnitudes and spectral shapes of the absorption coefficients of phytoplankton, detritus, and colored dissolved organic matter (CDOM) in a dynamic bay (Santos Bay) in southeastern Brazil in response to the contributions of the main estuarine channel and large tide variations, therefore in different time scales. Two strategies were adopted: (1) monthly year-round sampling in the estuarine channel and Santos Bay and (2) sampling in Santos Bay during spring/neap tides and cold/warm months. Chlorophyll-a concentration and CDOM absorption were higher during warm (wet) months, while the relative contribution of organic and inorganic particles was driven by neap/spring tide cycles. Salinity partially accounted for changes in optical variables, especially for CDOM absorption and total suspended matter (TSM) during cold months and neap tides, respectively. The spectral shapes of detritus and CDOM absorption showed relatively little variability for the entire dataset and were not considered feasible for monitoring purposes. The spectral shape of phytoplankton absorption (index of cell size) varied broadly, with no remarkable dependence on the sampling conditions. Comparison of absorption coefficients measured by the Quantitative Filter Technique (QFT) and Transmittance Reflectance (TR) method showed higher phytoplankton coefficients toward longer visible wavelengths (flatter spectra) and shallower slopes of detritus absorption yielded by the TR method. Our results also suggest that measurements at the near red spectral region result from not only scattering signals but also non-algal particle absorption. [Copyright &y& Elsevier]

BRS Savanna, a cross between V. Morales x IF200113, is a spring andan early-maturing six-rowed barley, widely adapted to irrigated areas of the savanna, in Central Brazil. It presents production stability and the industrial quality, grain yield and lodging resistance are high. It is suitable for cultivation in the states of GO, MG and DF [ABSTRACT FROM AUTHOR]

Aim: Clinical strains of Klebsiella quasipneumoniae subsp. similipneumoniae have been reported worldwide. Multidrug-resistant (MDR) hypermucoviscous (hm)/hypervirulent (hv) lineages have become a global problem for public health worldwide. Therefore, this study aimed to characterize by whole-genome sequencing a MDRhm/hv K. quasipneumoniae subsp. similipneumoniae SWT10 strain belonging to the new sequence type ST4417 isolated from a sewage treatment plant. Results: The SWT10 strain was recovered from a sewage treatment plant in Brazil and presented the hm and MDR phenotypes. Resistome analysis showed antimicrobial resistance genes associated with resistance to fluoroquinolones, b-lactams, tetracyclines, trimethoprim, aminoglycosides, sulfonamides, macrolides, and fosfomycin as well as several heavy metal resistance genes. Virulome analysis showed virulence factors related to hv lineages. Multilocus sequence typing analysis revealed the new ST4417, which was grouped in CC1584 by the minimum-spanning tree. Besides, five plasmid incompatibility groups, two prophage-related sequences, and 66 genomic islands were detected. Conclusion: This study reports for the first time the genome sequence of a MDR-hm/hv K. quasipneumoniae subsp. similipneumoniae recovered from the environment, which contributes to a better understanding about these lineages as well as for surveillance studies worldwide. [ABSTRACT FROM AUTHOR]

Whopping cough (or Pertussis) is an acute infectious respiratory disease caused by Bordetella pertussis bacteria. The disease is highly transmissible and can be fatal in children under two years old. Since the introduction of vaccine immunization in 1940, Pertussis incidence decreased worldwide. In Brazil, the immunization was introduced in 1977 using the whole cell (wP) vaccine. Despite the high vaccination coverage, an unexpected increase in the number of observed Pertussis cases was observed in 2012. In this year, 2257 cases were reported exceeding the average incidence rate of <1000 cases per year until 2010. This outbreak reached a peak level in 2014 and ended in 2018 according to the Brazilian National Surveillance System (SINAN). To understand the relationship between the outbreak and the vaccination, bacterial isolates (n = 136) from the Brazilian Midwest region obtained during the outbreak were submitted to genotyping of two vaccine loci: ptx P and fim 3. Most of isolates (102) were obtained from nursing children (29 days to 2 years old). Genotyping of 94 isolates revealed that fim 3–24/ ptx P-3 was the most prevalent genotype (68%) associated with the outbreak peak. Two additional genotypes were also observed: fim 3–1/ ptx P-3 (15%) and fim3 –3/ ptx P-3 (17%). Conversely, the fim 3–1/ ptx P-2 genotype, which is harbored by the strain used in the wP vaccine (Bp137), was not observed. These results showed that B. pertussis circulating strains in the outbreak analyzed were different from the strain used for Pertussis immunization in Brazil. These observations provide insights that could be used to target vaccination programs to prevent future whooping cough outbreaks in Brazil. • Whooping cough is an acute respiratory disease caused by Bordetella pertussis bacteria. • Since 1990, Brazil presents a high vaccine coverage to whooping cough. • A whooping cough outbreak occurred in Brazilian Midwest between 2012 and 2018. • fim 3–24/ ptx P-3 is the most frequent genotype observed in this occasion. • All isolates from this outbreak were from genotypes divergent from vaccinal strain. [ABSTRACT FROM AUTHOR]

Background: Many clinicians and researchers in Brazil consider the Neurological Developmental Exam (NDE), a valid and reliable assessment for Brazilian school-aged children. However, since its inception, several tests have emerged that, according to some researchers, provide more in-depth evaluation of motor ability and go beyond the detection of general motor status (soft neurological signs). Objectives: To highlight the limitations of the NDE as a motor skill assessment for first graders. Methods: Thirty-five children were compared on seven selected items of the NDE, seven of the Bruininks-Oseretsky Test (BOT), and seven of the Visual-Motor Integration test (VMI). Participants received a "pass" or "fail" score for each item, as prescribed by the respective test manual. Results: Chi-square and ANOVA results indicated that the vast majority of children (74%) passed the NDE items, whereas values for the other tests were 29% (BOT) and 20% (VMI). Analysis of specific categories (e.g. visual, fine, and gross motor coordination) revealed a similar outcome. Conclusions: Our data suggest that while the NDE may be a valid and reliable test for the detection of general motor status, its use as a diagnostic/remedial tool for identifying motor ability is questionable. One of our recommendations is the consideration of a revised NDE in light of the current needs of clinicians and researchers. [ABSTRACT FROM AUTHOR]