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• Gene expression in response to Cd: The study analyzed the expression levels of several genes (RAMP6, HIPP26, HIPP27, HMA2, and MAPK2) in response to CdCl2 exposure. The results showed an overall increase in gene expression with higher Cd concentrations, except for HMA2 at 1000 µM CdCl2. • MAPK2 expression variability: The expression of MAPK2 varied among kale varieties, with an overall decrease in Kale 23. This may be due to mutations in the MAPK2 primers and potential cell death in higher CdCl2 concentrations. • Role of RAMP6: RAMP6 was found to have a proportional increase in expression with increasing CdCl2 concentrations, suggesting its role in Cd metabolism in B. Oleracea. • HIPP26 and HIPP27 Potential Roles: While studies specific to B. Oleracea are lacking, findings from A. thaliana suggest that HIPP26 and HIPP27 may be involved in Cd influx in B. Oleracea, with HIPP27 possibly playing a role in Cd detoxification. • Kale varietal resistance: Domestic B. Oleracea kale varieties exhibited greater resistance to Cd stress compared to hybrid varieties, highlighting the potential for phytoremediation. Brassica oleracea var. acephala , commonly referred to as kale, is a well-documented plant species, a food crop but well recognized for its capacity to endure and manage the accumulation of heavy metals. In this research, the phytoremediation potential of kale was evaluated based on cadmium intake, utilizing three distinct kale varieties originating from Bosnia and Herzegovina. All kales were grown in controlled conditions, with different concentrations of cadmium (Cd), a known strong pollutant found in small concentrations in soil under normal environmental conditions. After the root length analysis and cadmium atomic spectrometry, we utilized quantitative PCR (qPCR) and cycle threshold (Ct) values to calculate the expression levels of five genes associated with Cd heavy metal response: Mitogen-activated protein kinase 2 (MAPK 2), Farnesylated protein 26 and 27 (HIPP 26 , HIPP 27), Natural resistance-associated macrophage protein 6 (RAMP 6), and Heavy metal accumulator 2 (HMA 2). The atomic reader's analysis of rising cadmium concentrations revealed a proportional decline in the length of kale roots. The gene expression levels corresponded to cadmium stress differently among varieties, but mostly showing notable up-regulations under Cd stress, indicating the strong Cd presence within the plant. This study demonstrated differences in gene expression behavior among three B. oleracea varieties from Bosnia and Herzegovina, indicating and filtering the Cd-resistant kale, and kale varieties suitable for phytoremediation. For the first time, such a study was conducted on kale varieties from Bosnia and Herzegovina, analyzing the impact of cadmium on the growth and resilience of these species. [ABSTRACT FROM AUTHOR]