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A comparison study was performed on anionic surfactant and phosphate concentrations between the entrance and the exit of Turkish Straits System (TSS). Surface water samples were collected at fall season in 2016 and the spring season in 2017. According to the values obtained from the samples taken from 6 stations at the entrance and 7 at the exit, anionic surfactant values were high at the exit of the TSS, while the phosphate concentrations were relatively lower. The high phosphate values at the entrance are related to the eutrophic feature of the Black Sea. Also, the location of these stations covers one end of the Canal Istanbul Project, which is planned to be built soon, so the data of this study will constitute an important database., (Copyright © 2020 Elsevier Ltd. All rights reserved.)