1. 26-Locus Y-STR typing in a Bhutanese population sample.
- Author
-
Parkin EJ, Kraayenbrink T, van Driem GL, Tshering Of Gaselô K, de Knijff P, and Jobling MA
- Subjects
- Amelogenin, Bhutan, DNA Fingerprinting, Dental Enamel Proteins genetics, Gene Frequency, Humans, Male, Polymerase Chain Reaction, Chromosomes, Human, Y, Genetics, Population, Tandem Repeat Sequences
- Abstract
26 Y chromosome short tandem repeat (STR) loci were amplified in a sample of 856 unrelated males from Bhutan, using two multiplex polymerase chain reaction (PCR) assays. The first multiplex is the Y-STR 20plex described by Butler et al. [J.M. Butler, R. Schoske, P.M. Vallone, M.C. Kline, A.J. Redd, M.F. Hammer, A novel multiplex for simultaneous amplification of 20 Y chromosome STR markers, Forensic Sci. Int. 129 (2002) 10-24], and the second is a novel (but overlapping) 14plex that targets six additional Y-STRs (DYS425, DYS434, DYS435, DYS436, DYS461, DYS462) and also amplifies the amelogenin locus. The 26-loci give a discriminating power of 0.9957, though even at this resolution one haplotype occurs 24 times. We identify novel alleles at five loci and microvariants at a further three, which were characterised by sequencing. Extended (11-locus) haplotypes for these samples have been submitted to the Y-STR Haplotype Reference Database (YHRD).
- Published
- 2006
- Full Text
- View/download PDF